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| Registros recuperados: 43 | |
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| Fagnani,Rafael; Schuck,Josiane; Botaro,Bruno Garcia; Santos,Fernando Cesar dos. |
| Abstract: The objective of this work was to evaluate the effect of the extended cold storage of raw milk on the kinetics of fermentation on yogurt production, as well as on the product’s microbiological and physicochemical properties during shelf life. Three treatments were evaluated: yogurts made with raw milk stored for 4, 72, and 168 hours. Kinetics of fermentation was assessed through the lactic acid production rate, growth rate of Streptococcus thermophilus and Lactobacillus bulgaricus, and time to reach pH 5.0 and 4.5. The physicochemical and microbiological characteristics of raw milk and yogurts were also analyzed during cold storage at 4°C. The microbial quality of raw milk was affected by the storage time of 168 hours, with a decreasing tendency... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus bulgaricus; Streptococcus thermophilus; Fermentation; Kinetics; Psychrotrophs.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000200104 |
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| Lalitendu Das; North Carolina State University Biological and Agricultural Engineering; Praveen Kolar; North Carolina State University; Jason Osborne; North Carolina State University Department of Statistics; John Classen; North Carolina State University Biological and Agricultural Engineering. |
| Swine farming emit several odorous volatile organic compounds, one of which is p-cresol. Considering the layout of a swine barn, adsorption is one of the most suitable technologies for mitigating organic pollutants. In this study, commercial granular activated carbon (GAC) was tested as an adsorbent for removing p-cresol from aqueous solution. The objectives were to: 1) determine the combined effect of temperature, pH, and adsorbent dose on adsorption, (2) investigate the effect of volatile fatty acids and aldehydes on adsorption of p-cresol, (3) determine adsorption kinetics and isotherms, (4) study the effect of solvent on adsorption, (5) propose a possible mechanism of adsorption, and (6) discuss practical implications and design calculations for... |
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Palavras-chave: Agricultural Engineering Adsorption; P-cresol; Activated carbon; Kinetics; Isotherms; RSM. |
| Ano: 2012 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2171 |
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| Cavalcante,D.R.; Perin,F.B.; Benedetti,E.. |
| Dry matter (DM) ruminal degradability of corn (Zea mays L.), millet (Pennisetum glaucum (L. R. Br.) and sorghum (Sorghum bicolor L. Moench.) was evaluated using in situ technique with samples in green chopped and ensiled forms. Two crossbred fistulated (live weight of 480kg) dry cows participated. Samples of six grams in each forage were incubated in the rumen for 6, 24 and 96 hours. We estimated time zero (t0) washing the bags in water and it was used to calculate solubility. The experimental design followed a randomized block design with a split plot. We compared the average of DM through the SNK test at 5% probability. The results of disappearance (%) of dry matter forages of green chopped and ensiled forms in 0, 6, 24 and 96 hours were respectively:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromatology plant; Corn; Kinetics; Millet; Sorghum. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100023 |
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| Adona,P.R.; Leal,C.L.V.. |
| The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011 |
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| Paca,Jan; Kosteckova,Alena; Pacova,Leona; Prell,Ales; Halecky,Martin; Paca Jr.,Jan; Stiborova,Marie; Kozliak,Evguenii; Soccol,Carlos Ricardo. |
| Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor, with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Respirometry; Kinetics; Phenol; Nutrition stress. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600030 |
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| Borderes,Josiane; Costa,Alessandra; Guedes,Alessandro; Tavares,Lorena Benathar Ballod. |
| The aim of this work was to study the production of compounds with antioxidant activity by Pycnoporus sanguineus when cultivated in submerged fermentation using a potato dextrose broth plus peptone medium. The study evaluated the biomass production, glucose consumption, variation of medium pH and antioxidant activity. The antioxidant potential was tested through the DPPH method and β-carotene / linoleic acid system with extracts obtained from the mycelium at different times of cultivation (5, 10, 15, 20, 25 and 30 days). Maximum kinetic values of specific growth rate (0.289 day-1), biomass productivity (0.698 g.L-1.day-1) and the yield of glucose conversion into biomass (26.24 g.g-1) were obtained during the exponential growth phase. The highest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycetes; Submerged culture; Kinetics; Antioxidant compounds. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600012 |
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| Granato,Daniel; Castro,Inar Alves de; Piekarski,Flávia Vilas Boas Wiecheteck; Benincá,Cristina; Masson,Maria Lucia. |
| This work was aimed at studying the influence of PFJ and/or sodium disulphite on Yacon pulp colour, and evaluating the sensory attributes of Yacon non-sugar pastes. A colour kinetic evaluation of browning in 11 treatments containing Yacon tubers, yellow passion fruit juice (PFJ) and/or sodium disulphite, over the course of 240 minutes was studied to inactivate the PPOs activity. The evaluation of the colour alterations of Yacon tuber by the addition of variable amounts of PFJ and sodium disulphite enabled identification of efficient treatments for the inhibition of browning: T7 (300 g kg -1 PFJ), T8 (400 g kg -1 PFJ), and T10 (150 g kg -1 PFJ + 0.25 g kg -1 sodium disulphite). These treatments presented high colour intensity (C*) and an insignificant (P... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Smallanthus sonchifolius; Diabetes; Passion fruit; Sensory analysis; Kinetics; Polyphenol oxidases. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100020 |
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| Neves,Valdir Augusto. |
| Soluble, ionically bound peroxidase (POD) and polyphenoloxidase (PPO) were extracted from the pulp of peach fruit during ripening at 20°C. Ionically bound form was purified 6.1-fold by DEAE-cellulose and Sephadex G-100 chromatography. The purified enzyme showed only one peak of activity on Sephadex G-100 and PAGE revealed that the enzyme was purified by the procedures adopted. The purified enzyme showed a molecular weight of 29000 Da, maximum activity at pH 5.0 and at 40ºC. The calculated apparent activation energy (Ea) for the reaction was10.04 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 75ºC with a fast inactivation at 75ºC. Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peach peroxidases; Ripening; Purification; Kinetics; Heat stability. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100002 |
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| Dikshit,Rashmi; Tallapragada,Padmavathi. |
| The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whereas the optimum temperature was 60°C. Enzyme kinetics studies with different concentration of pNPG showed the calculated value of Km approximately 0.89 mM with the non-linear regression and 0.98 mM with the linear regression techniques. The enzyme was predominantly inhibited by KCl (69.8%) and moderately inhibited by CaCl2(14.8%). Studies on the sensitivity for glucose showed that after 100 mM... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zymography; Agro-waste; Kinetics; Monascus sanguineus; PNPG. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200185 |
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| Lugon,J.R.; Pereira,G.R.M.; Strogoff-de-Matos,J.P.; Peixoto,A.J.. |
| Details about the acid-base changes in hemodialysis are scarce in the literature but are potentially relevant to adequate management of patients. We addressed the acid-base kinetics during hemodialysis and throughout the interdialytic period in a cross-sectional study of adults undergoing conventional hemodialysis. Samples for blood gas analysis were obtained from the arterial limb of the arteriovenous fistula before the first session of the week (HD1), immediately at the end of HD1, and on sequential collections at 15, 30, 45, 60, and 120 min post-HD1. Additional blood samples were collected after ∼20 h following the end of the first dialysis and immediately prior to the initiation of the second dialysis of the week. Thirty adult patients were analyzed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemodialysis; Renal replacement therapy; Kinetics; Adequacy of dialysis; Acid-base. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100611 |
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| Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O.. |
| Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602 |
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| Mukhtar,Hamid; Ikram-ul-Haq,. |
| We describe the simultaneous production of Bacillus subtilis based proteases and alpha amylase using a computer controlled laboratory scale 7.5 L batch bioreactor. The present strain is the first to be reported that concomitantly produces these two industrially important enzymes. The growth and sporulation of Bacillus subtilis was monitored and maximum production of alkaline protease and alpha amylase was found to coincide with maximum sporulation. Two types of proteases were detected in the fermentation broth; a neutral and an alkaline protease most active in a pH range of 7.0-8.0 and 8.0-10, respectively. Maximum production of proteases was observed at an incubation temperature of 37ºC while that of alpha amylase was observed at 40ºC. The optimum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Simultaneous; Sporulation; Kinetics; Fermentation; Agitation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300033 |
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| Registros recuperados: 43 | |
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