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Acute inflammatory response in Nile tilapia fed probiotic Lactobacillus plantarum in the diet - doi: 10.4025/actascibiolsci.v33i3.8011 Biological Sciences
Dotta, Geovana; Laboratório AQUOS-Sanidade de Organismos Aquáticos, Depto. de Aqüicultura, CCA, UFSC - Rod. Admar Gozaga 1346 - 88040-900 - Florianópolis, SC - Brasil; Mouriño, José Luis Pedreira; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Jatobá, Adolfo; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Burgos Morán, Ricardo Ernesto; Universidad Politécnica de Catalunya, Campus del Baix Llobregat, Av. del Canal Olímpic, s/n - 08860 - Castelldefels, Catalunya - España; Pilati, Celso; UFSC; Martins, Maurício Laterça; UFSC.
The present study evaluated the acute inflammatory response induced by carrageenin (500 µg) injected in the swim bladder of Nile tilapia, after fed or not probiotic supplemented diet. Fifty four fish were distributed in six treatments and three replicates: Group A: Fish fed unsupplemented diet: 0.5 mL saline-injected fish; fish injected with 500 µg carrageenin diluted in 0.5 mL saline; Non-injected. Group B: Fish fed probiotic supplemented diet: saline-injected fish; carrageenin-injected fish; Non-injected. Fifteen days after feeding the fish were injected with carrageenin or saline. After six hours, inflammatory exudate was collected, as well as the blood for hematocrit, red blood cell (RBC) and white blood cell (WBC) counts,...
Tipo: Composição bioquímica Palavras-chave: 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8011
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Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi Arq. Bras. Med. Vet. Zootec.
Vieira,F.N.; Buglione,C.C.; Mouriño,J.P.L.; Jatobá,A.; Martins,M.L.; Schleder,D.D.; Andreatta,E.R.; Barraco,M.A.; Vinatea,L.A..
The effect of a Lactobacillus plantarum-supplemented diet on shrimp growth, digestive tract bacterial microbiota, survival, and some hemato-immunological parameters after an experimental challenge with Vibrio harveyi was studied. No difference (P&gt;0.05) was observed in shrimp survival (80±6%) and final weight (6.63±0.56g) after 60 days feeding trial. Total bacteria count and Vibrio spp. count in the digestive tract were not diferent (P&gt;0.05) until day 40, but they were lower (P<0.05) in the probiotic-supplemented group on day 60. Total lactic bacteria in the shrimp digestive tract was higher after day 20 in the probiotic-supplemented group. Ten hours after V. harveyi challenge, survival of the probiotic-supplemented group (65.7±2.9%) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Lactobacillus plantarum; Intestinal microbiota; Immunostimulation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300019
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Cultivation of juvenile fat snook (Centropomus parallelus Poey, 1860) fed probiotic in laboratory conditions BABT
Barbosa,Moysés Cavichioli; Jatobá,Adolfo; Vieira,Felipe do Nascimento; Silva,Bruno Corrêa; Mourino,José Luiz Pedreira; Andreatta,Edemar Roberto; Seiffert,Walter Quadros; Cerqueira,Vinícius Ronzani.
The objective of this study was to investigate the growth of juvenile fat snook (Centropomus parallelus) in laboratory conditions when fed a diet supplemented with the probiotic Lactobacillus plantarum. Changes in the intestinal flora, haematological parameters and growth performance were assessed using 180 fishes (54.2 ± 13.4 g each). The fishes were subjected to two treatments divided into six cages: 1) feed supplemented with probiotic, and 2) feed without probiotic (control). The temperature, dissolved oxygen and salinity were maintained at 25 ± 1 ° C, 4.0 mg.L-1 and 33 ‰, respectively. After 10 weeks of culture, the fishes fed probiotic had reduced viable culturable heterotrophic bacteria and Vibrio spp. and increased lactic acid bacteria in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Hematology; Bacterial microbiota; Body index.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400020
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Produção de Lactobacillus plantarum em melaço de cana-de-açúcar BABT
Feltrin,Valdemar P.; Sant'Anna,Ernani S.; Porto,Anna C. S.; Torres,Regina C. O..
Comparative studies of Lactobacillus plantarum were carried out in MRS broth (control) and in 3% (w/v) sugar cane blackstrap molasses enriched as follows: 0.5% yeast extract, 0.5% ammonium citrate and 0.5% sodium acetate (Medium 1) and 0.5% yeast extract, 0.5% ammonium citrate, 0.5% sodium acetate, 0.5% ammonium phosphate, 0.2% ammonium citrate, 0.1% Tween 80 and 0.005% manganese sulphate (Medium 2). The experiment, was carried out at a 5 L fermentor, with 3.5 L as working volume. At the end of the fermentation period MRS presented the highest viable cells production (28.68 log CFU/mL) while in Medium 2 it was 25.80 and in Medium 1 19.36 log CFU/mL. Biomass productivity was 0.0865 g.L-1.h for MRS (control), 0.0768 and 0.0507 g.L-1.h for Medium 2 and Medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Fermentation; Molasses.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100015
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Combining a probiotic with organic salts presents synergistic in vitro inhibition against aquaculture bacterial pathogens BABT
Bolivar,Norha Constanza; Legarda,Esmeralda Chamorro; Seiffert,Walter Quadros; Andreatta,Edemar Roberto; Vieira,Felipe do Nascimento.
ABSTRACT This study aimed to evaluate 1) the in vitro effect of organic salts on the growth of the probiotic Lactobacillus plantarum and then 2) the combined use of a probiotic with organic salts on the in vitro inhibition of V. alginolyticus, A. hydrophila, E. coli, P. aeruginosa, and S. agalactiae. In vitro tests were performed with eight different organic salts, including butyrate, propionate, succinate, citrate, formate, fumarate, glutamate, and acetate, at two pH values (6.2 and 7.1) to determine their effect on the growth kinetics of L. plantarum. In addition, each organic salt was tested alone and in combination with L. plantarum to evaluate the inhibitory effect against the pathogenic bacteria noted above in either condition. Sodium citrate and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Sodium butyrate; Sodium propionate; Aquaculture pathogenic bacteria.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100415
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Molecular characterization of lactobacilli isolated from fermented idli batter BJM
Agaliya,Perumal Jayaprabha; Jeevaratnam,Kadirvelu.
Lactic acid bacteria are non pathogenic organism widely distributed in nature typically involved in a large number of spontaneous food fermentation. The purpose of this study was to characterize the bacteriocinogenic lactobacilli from fermented idli batter which can find application in biopreservation and biomedicine. Eight most promising lactobacilli were chosen from twenty two isolates based on their spectrum of activity against other lactic acid bacteria and pathogens. The eight lactobacilli were characterized based on the various classical phenotypic tests, physiological tests and biochemical tests including various carbohydrate utilization profiles. All isolates were homo fermentative, catalase, and gelatin negative. Molecular characterization was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Idli batter; Bacteriocin; Lactobacillus plantarum subsp. argentoratensis; Lactobacillus plantarum; Lactobacillus pentosus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400025
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Screening and characterization of lactic acid bacterial strains that produce fermented milk and reduce cholesterol levels BJM
Guan,Xuefang; Xu,Qingxian; Zheng,Yi; Qian,Lei; Lin,Bin.
ABSTRACT Objective To screen for and characterize lactic acid bacteria strains with the ability to produce fermented milk and reduce cholesterol levels. Methods The strains were isolated from traditional fermented milk in China. In vitro and in vivo evaluation of cholesterol-reduction were used to identify and verify strains of interest. Characteristics were analyzed using spectrophotometry and plate counting assays. Results The isolate HLX37 consistently produced fermented milk with strong cholesterol-reducing properties was identified as Lactobacillus plantarum (accession number: KR105940) and was thus selected for further study. The cholesterol reduction by strain HLX37 was 45.84%. The isolates were acid-tolerant at pH 2.5 and bile-tolerant at 0.5%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented milk; Lactobacillus plantarum; Cholesterol; Rat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400730
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Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31 BJM
Todorov,Svetoslav D.; Vaz-Velho,Manuela; Gibbs,Paul.
Two methods of purification of the plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31 are used in this study - the method of ammonium sulfate precipitation, Sep-pack C18 cartridge and reverse-phase HPLC chromatography on C18 Nucleosil column, and the method of direct purification by cation exchange SP Sepharose Fast Flow column Amersham (Pharmacia Biotech). The purity of the products from the two experimental protocols are examined for their molecular weight, aminoacid composition and sequence. Comparison of results show that the plantaricins purified with the two methods are identical. Both methods may be used to purify plantaricin ST31. Comparison of the yield in the purification protocols is 0.8% in the HPLC experimental protocol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Bacteriocin; Plantaricin; HPLC; Cation-exchange chromatography (IEX).
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100026
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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats BJM
Han,Xufeng; Wang,Lei; Li,Wei; Li,Bibo; Yang,Yuxin; Yan,Hailong; Qu,Lei; Chen,Yulin.
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green fluorescent protein; Gastro-intestinal tract; Lactobacillus plantarum; Goat.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849
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Survival of Listeria monocytogenes in low acid italian sausage produced under brazilian conditions BJM
Degenhardt,Roberto; Sant'Anna,Ernani S.
Dry sausages have been considered ready-to-eat products with low risk of causing listeriosis due to the hurdles created during the manufacturing process such as low pH and a w, high salt concentration and presence of lactic acid bacteria (LAB). However, several studies have detected survival of Listeria monocytogenes in these products and also shown that process parameters, LAB and L. monocytogenes strains directly influence the results. In this work, survival of the pathogen in sausages prepared with three different formulations (one standard formulation, one formulation added of Lactobacillus plantarum and one added of 2% sodium lactate), using the manufacturing process usually employed in Brazil, was evaluated. Naturally contaminated sausages presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Lactobacillus plantarum; Fermented dry sausage; Survival; Brazilian salami.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200024
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Bacteriocins from Lactobacillus plantarum production, genetic organization and mode of action: produção, organização genética e modo de ação BJM
Todorov,Svetoslav D..
Bacteriocins are biologically active proteins or protein complexes that display a bactericidal mode of action towards usually closely related species. Numerous strains of bacteriocin producing Lactobacillus plantarum have been isolated in the last two decades from different ecological niches including meat, fish, fruits, vegetables, and milk and cereal products. Several of these plantaricins have been characterized and the aminoacid sequence determined. Different aspects of the mode of action, fermentation optimization and genetic organization of the bacteriocin operon have been studied. However, numerous of bacteriocins produced by different Lactobacillus plantarum strains have not been fully characterized. In this article, a brief overview of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Plantaricin; Mode of action; Genetic organization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200001
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Silagens de milho inoculadas microbiologicamente em diferentes estádios de maturidade: perdas fermentativas, composição bromatológica e digestibilidade in vitro Ciência Rural
Rabelo,Carlos Henrique Silveira; Rezende,Adauton Vilela de; Rabelo,Flávio Henrique Silveira; Nogueira,Denismar Alves; Senedese,Simone Silvia; Vieira,Paulo de Figueiredo; Bernardes,Célio Luiz; Carvalho,Arthur.
Objetivou-se com este estudo avaliar as perdas fermentativas, composição bromatológica e digestibilidade in vitro da matéria orgânica de silagens de milho produzidas em diferentes estádios de maturidade, inoculadas microbiologicamente. Aplicaram-se dois inoculantes comerciais nas silagens produzidas nos estádios SLL, 1/3 LL, 1/2 LL, 2/3 LL e CN, permanecendo ainda um tratamento sem inoculação (silagem controle), configurando um esquema fatorial 3x5. A inoculação com BAL resultou em menores perdas fermentativas (P=0,0348), ao passo que silagens produzidas com plantas mais secas também apresentam menores perdas de MS (P<0,01). A inoculação das silagens resultou em maiores concentrações de PB nas silagens produzidas nos estádios SLL, 2/3 LL e CN...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus plantarum; Pediococcus spp.; Linha de leite.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000200028
Registros recuperados: 32
Primeira ... 12 ... Última
 

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