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Registros recuperados: 25
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Bovine placentome preservation for light microscopy evaluation Arq. Bras. Med. Vet. Zootec.
Martins,E.; Martins,V.M.V.; Marques Júnior,A.P.; Vasconcelos,A.C.; Chiarini-Garcia,H.; Malard,P.F..
This work evaluates the preservation of bovine placentomes using paraformaldehyde and glutaraldehyde at 4% and solutions of Karnovsky, Bouin and Carnoy for 4, 12 and 24 hours fixation, at room temperature or at 4ºC, before inclusion in plastic resin and paraffin. The best preservation of the specimens was obtained after 12 hours of fixation with those solutions containing aldehydes. With Bouin solution the best time of fixation was 4 hours, while for Carnoy solution the best time of fixation was 24 hours. Inclusion in plastic resin gave better results than in paraffin, and the temperature did not influence the quality of preservation of the specimens of bovine placentomes for evaluation with light microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Placentome; Light microscopy.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000200006
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Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy Biol. Res.
García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A.
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in...
Tipo: Journal article Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006
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Microscopic Studies on Erythrocytes of Channa punctata Exposed to Commercial Grade Lindane BABT
Bhattacharjee,Debasish; Das,Suchismita.
ABSTRACT Light and scanning electron microscopic studies of erythrocytes of Channa punctata exposed to two sublethal doses of lindane over a period of 21 days revealed myriads of anomalies. High frequency of micronucleus appeared progressively in the erythrocyte, ranging from 3.8-7.5%, when monitored weekly for 21 d at 0.25 g/L and 1.0-3.0% at 0.025 g/L lindane. Prominent structural anomalies of erythrocytes included abnormal shapes, vacuolation, membrane invagination and disintegration. At higher dose and at later duration, a unique tendency of abnormal cells to stick together was observed. It could be concluded that commercial formulation of lindane could induce genotoxicity and structural anomalies in the erythrocytes of fish.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RBC; SEM; Light microscopy; Micronucleus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100449
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Radioautographology: the proposal of a new concept BJMBR
Nagata,T..
A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001
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Methods for glomerular quantification in dogs: a comparative study Ciência Rural
Costa,Caio Augusto Leles; Lima,Charles Silva de; Uscategui,Ricardo Andres Ramirez; Silva,Gyl Eanes Barros; Crivellenti,Leandro Zuccolotto.
ABSTRACT: It was evaluated the different methodologies for glomerular enumeration described in the literature in a quantitative, double-blind study on the number of glomeruli present in kidney biopsy specimens obtained using a Tru-cut-type biopsy needle. Eighteen samples were taken (n=18) from various regions of canine kidneys harvested immediately after euthanasia. Sample collection was carried out using Tru-cut-type, semi-automated, 16 gauge needles. Reading and evaluation of the specimens were performed independently by four researchers by means of eye loupe inspection, light microscopy, light microscopy with a reduced condenser aperture, and dissection microscopy. Samples were also subjected to histopathological evaluation for comparison purposes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glomerulopathy; Histopathology; Light microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300600
Registros recuperados: 25
Primeira ... 12 ... Última
 

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