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C-Banding patterns and meiotic behavior in Hypsiboas pulchellus and H. Cordobae (anura, hylidae) JBAG
Baraquet,M; Salas,N.E; Martino,A.L.
In this work, we observed and evaluated C-banding patterns and meiotic behaviour of Hypsiboas pulchellus and H. cordobae. Twenty metaphasic cells per individual were analyzed, which were obtained from intestinal and testis cells using conventional and C-banding techniques. The two species presented 2n= 24 chromosomes. Pairs 1 and 8 to 12 were metacentric whereas pairs 2 to 7 were submetacentric. The centromeric region of all chromosomes was positively C-banded but the C-banding pattern varied between species. At meiosis, cells with 12 bivalents were observed. Bivalents usually presented two terminal chiasmata, although the largest pair presented only one terminal chiasma. The cytogenetic data reconfirms the diploid somatic chromosome number of H....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotypes; C-banding; Meiosis; Hypsiboas pulchellus; Hypsiboas cordobae.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000100005
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Variaciones meióticas y evolución cromosómica en insectos y arácnidos con cromosomas holocinéticos JBAG
Mola,L. M; Rebagliati,P. J; Rodríguez Gil,S. G; Adilardi,R. S.
Los estudios citogenéticos en grupos modelo de insectos (Odonata y Heteroptera) y arácnidos (Dysderoidea y Buthidae) con cromosomas holocinéticos nos han permitido determinar diferencias en el comportamiento meiótico de autosomas y cromosomas sexuales (meiosis pre- o post-reduccional, quiasmática o aquiasmática, actividad telocinética u holocinética) y en la frecuencia y distribución de las mutaciones cromosómicas (fusiones, fragmentaciones y translocaciones en homocigosis o heterocigosis) en la evolución del cariotipo.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cromosomas holocinéticos; Meiosis; Evolución cromosómica; Odonata; Heteroptera; Dysderoidea; Buthidae.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000100012
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Pollen viability and meiotic irregularities in a collection of Stevia rebaudiana (Bertoni) Bertoni cultivated in Tucumán, Argentina JBAG
Budeguer,C.J; Camadro,E.L; Erazzú,L.E.
Stevia rebaudiana (Bertoni) Bertoni (2n=2x=22) (Asteraceae family) is a species of economic value due to the presence of steviol glycosides in leaves -mainly stevioside and rebaudioside A- which are non-caloric sugars. In 2013, a collection of plants (genotypes) from four contrasting environments was established in Tucuman, Argentina, for evaluation under local conditions and, eventually, breeding purposes. As a first step, pollen viability and meiosis was studied in a sample of 56 plants. The percentage of pollen viability varied from medium (69.4%) to high (99.6%) in 52 of them, but from low (36.5%) to relatively low (51.5%) in the remaining four. The latter four plants also presented pollen grains of heterogeneous size, which were classified as n...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stevia; Pollen viability; Meiosis; Sporads.
Ano: 2018 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000200005
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Use of 2n pollen in generating interspecific derivatives of groundnut Open Agri
Mallikarjuna, N..
Palavras-chave: Genomes; Polyploidy; Nucleus; Hybrids; Chromosome number; Chromosomes; Mosses; Meiosis; Cytogenetics; DNA.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3176
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Abnormal meiotic behavior in three species of Crotalaria PAB
Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma.
The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012
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Estudio de los cromosomas mitóticos y meióticos del sotol (Dasylirion cedrosanum Trel.) Phyton
Hernández-Quintero,JD; Reyes-Valdés,MH; Mendoza-Rodríguez,DV; Gómez-Martínez,M; Rodríguez-Herrera,R.
El género Dasylirion es un grupo de plantas típicas del Desierto Chihuahuense, perennes, de un comportamiento sexual dioico y denominadas comúnmente sotoles. Es un género poco estudiado desde el punto de vista biológico y se desconocen las bases de su dinámica reproductiva. En este trabajo se estudió el número cromosómico y el comportamiento meiótico de Dasylirion cedrosanum en el municipio de Saltillo, Coahuila, una localidad del noreste de México. Para la preparación de cromosomas mitóticos se utilizó una técnica basada en un tratamiento enzimático con pectoliasa y celulasa, así como tinción con acetocarmín. Para el estudio meiótico se recolectaron botones florales masculinos, se fijaron y se tiñeron con el mismo colorante. Como resultado, se reportan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dasylirion cedrosanum; Dioecia; Cromosomas; Mitosis; Meiosis.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000100015
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New contributions to the study of Corixoidea: cytogenetic characterization of three species of Sigara from Argentina and the plausible mechanisms of karyotype evolution within Nepomorpha Rev. Soc. Entomol. Argent.
Bressa,María José; Papeschi,Alba Graciela.
Cytogenetic studies in Heteroptera contribute to the analysis of evolutionary trends within the group. Heteroptera are characterized by the possession of holokinetic chromosomes, different sex chromosome mechanisms and a pair of m chromosomes in some species. In the present work, the male karyotype and meiosis in Sigara denseconscripta (Breddin), S. chrostowskii Jaczewski, and S. rubyae (Hungerford) are described. The three species share a diploid chromosome number of 2n= 24 with a pair of m chromosomes and an XY/XX sex chromosome system. With this study the chromosome number of 30 species of Corixoidea are known and the modal karyotype is 2n= 20+2m+XY in males. The available cytogenetic information in Heteroptera led us to suggest that the presence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Heteroptera; Holokinetic chromosomes; Meiosis; M chromosomes.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802007000200010
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Influence of high temperature on the reproductive biology of dry edible bean (Phaseolus vulgaris L.) Scientia Agricola
Silva,Daiana Alves da; Pinto-Maglio,Cecília Alzira Ferreira; Oliveira,Érica Cristina de; Reis,Raquel Luiza de Moura dos; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando.
ABSTRACT: The aim of this study was to investigate the effect of heat stress on 12 bean genotypes through the analysis of their reproductive biology in terms of flowering, pollen viability, meiotic behavior, and production. Plants were grown in a climate chamber at 25-20 °C (day and night) and at a high temperature treatment 37-26 °C (day and night) from the vegetative (V4) development stage to physiological maturity. The experimental design was 2 × 12 factorial arrangement with six replications and the factors consisted of heat treatments and genotypes. In three replications, the number of newly opened flowers was checked daily. At physiological maturity, the following traits were evaluated: percentage of pod set, number of pods, number of viable seeds,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Common bean; Heat stress; Pollen viability; Meiosis; Seed yield.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000301100
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Interspecific hybrids between Paspalum plicatulum and P. oteroi: a key tool for forage breeding Scientia Agricola
Novo,Patricia Elda; Valls,José Francisco Montenegro; Galdeano,Florencia; Honfi,Ana Isabel; Espinoza,Francisco; Quarin,Camilo Luis.
ABSTRACT Grama-tio-pedro (Paspalum oteroi Swallen) is a rare stoloniferous grass of the Plicatula group of Paspalum, well adapted to continuous grazing in areas subject to seasonal flooding in the Pantanal region, in central western Brazil. The species is a facultative apomictic (asexual reproduction by seed) tetraploid, sporadically cultivated on Pantanal farms, propagated either by cuttings or seed. Due to its potential for extensive cultivation and forage quality, Grama-tio-pedro appears as a candidate for genetic improvement within the Plicatula group through plant breeding. We used a colchicine-induced sexual autotetraploid genotype of P. plicatulum Michx. to obtain interspecific hybrids using the apomictic species, P. oteroi, as pollen donor. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA content; Apomixis; Fertility; Meiosis; Reproductive mode.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000400356
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Meiotic behavior of interspecific hybrids between artificially tetraploidized sexual Brachiaria ruziziensis and tetraploid apomictic B. brizantha (Poaceae) Scientia Agricola
Felismino,Mariana Ferrari; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
The meiotic behavior of four interspecific promising hybrids was evaluated by conventional cytological methods. The female genitors were two artificially tetraploidized sexual accessions of B. ruziziensis (R41 and R44, 2n = 4χ = 36), which were crossed to an agronomically superior natural tetraploid apomictic genotype of B. brizantha (B140 - BRA003395). Three of them (HBGC313, HBGC 315, and HBGC324) were sexual and one (HBGC325) apomictic. Analyses of some cells in diakinesis revealed multivalent chromosome configurations, suggesting that genetic recombination and introgression of some genes could be present. The four hybrids had different types of meiotic abnormalities at various frequencies. Abnormalities related to irregular chromosome segregation due...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria; Brazil; Cytogenetics; Forage grasses; Meiosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000200010
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Microsporogenesis in Brachiaria bovonei (Chiov.) Robyns and B. subulifolia (Mez) Clayton (Poaceae) Scientia Agricola
Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
Some African species of Brachiaria have been introduced into the Americas and became the most important forage for pastures in the tropics. New cultivars can be obtained either from direct selections from the natural existing variability in the germplasm collections or from interspecific hybridizations. Polyploidy is predominant in the genus Brachiaria and correlated with apomixis which complicates hybridization. The objective of cytological studies underway on the Brachiaria germplasm collection at Embrapa Beef Cattle is to determine the chromosome number and meiotic behavior of accessions. For the breeding of Brachiaria species, compatible sexual and apomictic accessions need to be identified. Microsporogenesis was evaluated in two accessions of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria; Meiosis; Chromosome number; Polyploidy; Breeding program.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500015
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Comportamiento meiótico de diferentes especies de lulo, Solanum sp Acta Agron. (Palmira)
Pareja Ordóñez,Nancy Maricela; Santacruz Ordóñez,Nazira Katherine; Ordóñez Jurado,Héctor Ramiro; Lagos Burbano,Tulio César.
Se realizó un análisis del comportamiento meiótico de las especies de lulo S. hirtum, S. quitoense y S. sessiliflorum, siguiendo la metodología convencional para los estudios de microsporogénesis. Se tomaron botones florales en diferentes estados de desarrollo, fijándolos por 24 horas en una solución de tres partes de etanol por una parte de ácido acético, saturada con trazas de cristales de cloruro férrico. Para la preparación de las placas se siguió la técnica de aplastamiento, se liberaron las células madres del grano de polen y finalmente se hicieron las observaciones bajo microscopía de luz. El análisis mostró que la meiosis se presenta en longitudes de antera que van desde los 2,79 mm hasta los 4,45 mm. La normalidad meiótica fue del 100%, tanto para...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meiosis; Variabilidad; Microsporogénesis; Índice meiótico; Normalidad meiótica.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122010000400002
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LA COMPETENCIA DEL OVOCITO: QUÉ, CÓMO Y CUÁNDO Acta biol.Colomb.
TARAZONA¹,ARIEL; LÓPEZ,ANGELA; OLIVERA-ANGEL,MARTHA.
La producción de embriones in vitro ha sido una herramienta útil para el desarrollo de técnicas como la clonación y la transgénesis, que han revolucionado los campos de la ciencia y la tecnología. La técnica básica para la maduración, fertilización y cultivo in vitro de los embriones produce, desde sus inicios, bajas tasas de desarrollo hasta el estadío de blastocisto con índices reducidos de viabilidad de los mismos. Los avances en el conocimiento de la dinámica celular durante estos procesos condujo a la hipótesis, de que la maduración, entendida como el reinicio del ciclo meiótico, la reorganización del ooplasma y la regulación de la expresión génica, juegan un papel fundamental para sustentar el desarrollo temprano de los embriones, esta capacidad de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Complejo folicular; Maduración; Meiosis; Ooplasma.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300001
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Meiotic behavior in Panicum maximum Jacq. (Poaceae: Panicoideae: Paniceae): hybrids and their genitors - doi: 10.4025/actasciagron.v32i3.6461 Agronomy
Pessim, Cleide; UEM; Pagliarini, Maria Suely; UEM; Jank, Liana; Embrapa; Kaneshima, Alice Maria de Souza; Universidade Estadual de Maringá; Bonato, Andréa Beatriz Mendes; Universidade Estadual de Maringá.
Panicum maximum Jacq. is one of the most cultivated grasses in the world. The intraspecific hybridization breeding program underway at Embrapa Beef Cattle aims at combining several agronomic characteristics in a single plant with high seed production. Four intraspecific hybrids originated from the cross between sexual tetraploid female plants with apomictic tetraploid male plants, including the genitors, were cytologically evaluated in the meiotic process throughout conventional methodology. Hybrids and genitors showed high meiotic stability. The percentage of abnormal cells among them ranged from 6.7 to 14.2%. Abnormalities recorded were irregular chromosome segregation, chromosome stickiness, and absence of the first cytokinesis, but the latter did not...
Palavras-chave: 5.01.03.05-9 breeding program; Meiosis; Intraspecific hybrids; Panicum maximum 5.01.03.05-9 breeding program; Meiosis; Intraspecific hybrids; Panicum maximum.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6461
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Comportamento mitótico e meiótico de cromossomos holocêntricos &gama; irradiados de Rhynchospora pubera (Cyperaceae) Biological Sciences
Vanzela, André Luís Laforga; UEL; Colaço, Waldeciro; UFPE.
Para avaliar a ocorrência de cinetócoros difusos em Rhynchospora (Cyperaceae), cromossomos de R. pubera foram fragmentados ou fundidos por irradiação &gama;. Este material foi investigado nas gerações celulares subseqüentes, em ambas, mitose e meiose. Alterações no número e no tamanho dos cromossomos foram detectadas, mas o comportamento dos cromossomos irradiados foi normal e nenhum micronúcleo foi observado. Todos os dados apontam para a presença de cinetócoros difusos em R. pubera.
Palavras-chave: 2.00.00.00-6 Ciências Biológicas Cyperaceae; Holocentric chromosomes; Meiosis; Mitosis; Rhynchospora 2.00.00.00-6 Ciências Biológicas.
Ano: 2002 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2364
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Botanical aspects of Heteropterys umbellata (Malpighiaceae): a cytological and palynological approach Anais da ABC (AABC)
Sousa,Saulo M.; Reis,Aryane C.; Gomes,Shaiany S.L.; Bernardo,Karina B.; Salimena,Fátima R.G.; Viccini,Lyderson F..
The genus Heteropterys is one of the major genera in Malpighiaceae. However, few cytological and palynological studies were reported. The present work described for the first time the chromosome number, heterochromatin pattern, meiotic behavior, pollen viability and palynological aspects of Heteropterys ubellata, a very spread species. One large Brazilian population was evaluated using conventional techniques for meiotic studies and acetolyse to access the pollen morphology. The species showed 2n = 20 chromosomes, normal meiotic development and viable pollens. Great blocks of heterochromatin were observed around the centromers. DAPI staining was positive for centroeric heterochroatin, hile CMA3 ark as observed just at terinal regions of one pair of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; Heterochromatin pattern; Heteropterys umbellata; Malpighiaceae; Meiosis; Pollen viability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400009
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes Arq. Bras. Med. Vet. Zootec.
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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Synaptonemal complexes and XY behavior in two species of argentinian armadillos: Chaetophractus villosus and Dasypus hybridus (Xenarthra, Dasypodidae) Biocell
Sciurano,R. B.; Merani,M.S.; Bustos,Jimena; Solari,A.J..
Spermatocytes from the two armadillo species, C. villosus and D. hybridus were studied in microspreads for synaptonemal complexes (SCs) and in thin sections for electron microscopy (EM). The complete SC karyotype generally agrees with previous reports on mitotic chromosomes, except for the sex chromosomes. The X chromosome is submetacentric in both species and the Y is the shortest one in C. villosus and the second shortest in D. hybridus, and an extremely acrocentric one. A SC is formed along the total length of the Y chromosome, and this SC persists along all the pachytene substages. A single ecombination nodule (RN) is located in the region of the SC nearest to the attachment to the nuclear envelope. The lateral element (LE) of the X axis in the SC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xenarthra; XY chromosomes; Meiosis; Synaptonemal complexes.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100009
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Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho &amp; Hiern) Stapf (Gramineae) Biocell
Sayuri Utsunomiya,Karina; Suely Paglirini,Maria; Borges do Valle,Cacilda.
The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho &amp; Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x = 36). Chromosomes paired preferentially as bivalents, but quadrivalents were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria nigropedata; Chromosome number; Meiosis; Polyploidy; Apomixis; Grasses.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300007
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A male-sterile mutation in soybean (Glycine max) affecting chromosome arrangement in metaphase plate and cytokinesis Biocell
Pires Bione,Nilton Cesar; Suely Pagliarini,Maria; Alves de Almeida,Leones.
A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa - National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis after telophase II. Instead of the typical tetrads of microspores, four nucleate coenocytic microspores were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Meiosis; Metaphase plate; Cytokinesis; Male sterility.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200004
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