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Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney ArchiMer
Takai, Ken; Miyazaki, Masayuki; Hirayama, Hisako; Nakagawa, Satoshi; Querellou, Joel; Godfroy, Anne.
P>Two novel, thermophilic piezophiles, capable of chemolithoautotrophic growth, are successfully cultivated and isolated from a black smoker chimney at the TAG field (Mid Atlantic Ridge: MAR) by using a piezophilic cultivation technique. Both strains (strains 106 and 108) represent dominant cultivated populations of the microbial communities in the chimney surface habitat. Strain 106 represents typically thin, long spiral cells under the piezophilic growth condition but short bent cells under the non-piezophilic condition. It is a strictly chemolithoautotrophic gammaproteobacterium using reduced sulfur compounds as the electron donors, and nitrate and O-2 as the electron acceptors. Based on the 16S rRNA gene sequence, strain 106 would represent a novel...
Tipo: Text Palavras-chave: Sp. nov.; Ecological signifiance; Hydrostatic pressure; Subsurface biosphere; Base composition; High temperature; Membrane lipids; Gen. nov.; Microorganisms; Diversity.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6652.pdf
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Geoglobus acetivorans sp nov., an iron(III)-reducing archaeon from a deep-sea hydrothermal vent ArchiMer
Slobodkina, G. B.; Kolganova, T. V.; Querellou, Joel; Bonch-osmolovskaya, E. A.; Slobodkin, A. I..
A hyperthermophilic, anaerobic, dissimilatory Fe(III)-reducing, facultatively chemolithoautotrophic archaeon (strain SBH6(T)) was isolated from a hydrothermal sample collected from the deepest of the known World Ocean hydrothermal fields, Ashadze field (12 degrees 58' 21 '' N 44 degrees 51' 47 '' W) on the Mid-Atlantic Ridge, at a depth of 4100 m. The strain was enriched using acetate as the electron donor and Fe(III) oxide as the electron acceptor. Cells of strain SBH6(T) were irregular cocci, 0.3-0.5 mu m in diameter. The temperature range for growth was 50-85 degrees C, with an optimum at 81 degrees C. The pH range for growth was 5.0-7.5, with an optimum at pH 6.8. Growth of SBH6(T) was observed at NaCl concentrations ranging from 1 to 6 % (w/v) with an...
Tipo: Text Palavras-chave: Dissimilatory reduction; Microorganisms; Gradient.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6977.pdf
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Evolution de la communaute bacterienne heterotrophe de l'eau de mer lors d'une experience d'aquaculture de crevettes peneides en systemes clos ArchiMer
Sohier, L; Bianchi, M.
The quantitative and qualitative evolution of the heterotrophic bacterial community of sea water was studied throughout an experimental rearing of Penaeus japonicus in closed systems. Cluster analysis was realised on 417 strains coming from water sampled at different moments of the experiment. This collection includes 40 strains coming from a mussel used for diet and 40 strains coming from the digestive tract of one prawn after six months rearing. Quantitative and qualitative studies have both shown a similar evolution of heterotrophic microflora whatever the initial animal load was. The dendrogram obtained led to a description of characteristics of the various populations constituting the heterotrophic bacterial community of the water environment...
Tipo: Text Palavras-chave: Penaeus japonicus; Malacostraca; Bacteria; Recirculating systems; Numerical taxonomy; Temporal variations; Microorganisms; Shrimp culture; Heterotrophic organisms.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-972.pdf
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Etude des liens trophiques au sein d'une communaute bacterienne selectionnee a partir d'un sediment marin sur le cyclohexene-4 dicarboximide-1,2 (THPI) ArchiMer
Terrom, G; Gil, G; Lepetit, J.
A bacterial community has been selected from a marine sediment on 4-cyclohexene-1,2 dicarboximide or THPI (fungicide). This community consists of nine strains. The "principal" strain, Photobacterium mandapamensis , degrades THPI by different metabolic pathways many of which require co-oxidative sequences. The other eight "auxiliary" strains occur at different states along the trophic chain formed by the "principal" strain, so increasing velocity and intensity of the THPI biodegradation.
Tipo: Text Palavras-chave: Bacteria; Marine pollution; Microorganisms; Metabolism; Fungicides; Biodegradation; Sediment pollution; Trophic relationships.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1021.pdf
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Fecal contamination in coastal areas: An engineering approach ArchiMer
Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf
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Nutrition experimentale: Problemes methodologiques lies a l'utilisation des microorganismes comme source trophique ArchiMer
Guidi, L.
The present study deals with technical problems encountered under lab conditions in studies of trophic relations between deposit-feeding invertebrates and microorganisms. Using 2 pure cultures of Pseudomonas and Navicula , it was shown that these microorganisms will attach to artificial (glass beads) and natural (sand grains) substrates. Best microbial attachment was observed on sand (up to 4.8 x 10 super(8) cells.g super(-1) dry wt.). The radioactive labelling of the microorganisms depends not only on the form under which the isotope ( super(14)C) is supplied to the cultures, but also on the time and the duration of the labelling. 70% of the isotope was incorporated in a stable manner by the diatoms. The bacteria incorporated, at most, 34% of the...
Tipo: Text Palavras-chave: Navicula; Pseudomonas; Bacillariophyceae; Algae; Bacteria; Biological attachment; Radioactive tracers; Methodology; Microorganisms; Trophic relationships; Microbiological culture; Zoobenthos; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-987.pdf
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Ecophysiological studies on luminous bacteria associated with marine gastropods. ArchiMer
Ramesh, A; Venugopalan, V.
Information available on the association of luminous bacteria with marine invertebrates is limited. Hence qualitative and quantitative investigations were undertaken with a view to understand the distribution of luminous microflora in relation to the environmental factors and also in the gut of two marine gastropods, Aplysia benedicti Eliot and Bullia tranquebarica (Roding) from Porto Novo (11 degree 29'N, 79 degree 46'E) waters of the East coast of India. The gut of the gastropods harboured maximum number of luminous bacteria when compared to the water and sediment. Qualitative analysis revealed the presence of two luminous bacterial components, viz. Vibrio harveyi and V. fishceri. V. fischeri was not found in association with A. benedicti . The isolates...
Tipo: Text Palavras-chave: Gastropoda; Vibrio fischeri; Vibrio harveyi; Bioluminescence; Bacteria; Physicochemical properties; Environmental factors; Microorganisms; Luminous organisms; Interspecific relationships; Ecophysiology.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-993.pdf
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Interactions of deep-sea vent invertebrates with their environment: The case of Rimicaris exoculata ArchiMer
Schmidt, Caroline; Le Bris, Nadine; Gaill, F.
The vent shrimp Rimicaris exoculata thrives around many hydrothermal vent sites along the Mid-Atlantic Ridge (MAR), where it aggregates into dense swarms. In contrast to hydrothermal vent fields at the East Pacific Rise (EPR), where the biomass is dominated by tubeworms, clams, and mussels, this shrimp is one of the major animal species at MAR vents. These animals are found in the dynamic mixing interface between cold oxygenated seawater and hot, reduced hydrothermal vent fluid. The adaptation of this shrimp to the hostile deep-sea hydrothermal environment and its survival strategy has been investigated since their discovery at the TAG site in the late 1980s. Rimicaris exoculata is now known to colonize black smoker complexes along the MAR in the...
Tipo: Text Palavras-chave: Extreme environments; Microorganisms; Chemosynthesis; Biomineralization; Symbiosis; Deep sea; Hydrothermal vents; Mid Atlantic Ridge; Shrimp.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3925.pdf
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Etude des effets des hautes pressions hydrostatiques sur Pyrococcus yayanosii, un piézophile extrême par une approche multi -”omics” ArchiMer
Michoud, Grégoire.
Since the discovery of hydrothermal vents in 1977, a small number of studies have allowed the isolation and characterization of microorganisms that can withstand high pressures and temperatures. Among these, Pyrococcus yayanosii, a hyperthermophilic archaea of the order Thermococcales, came from the Ashadze site (Mid-Atlantic Ridge) at 4100 m depth. This species represents the first organism both hyperthermophilic and strict piezophilic described to date. It cannot divide at pressures below 20 MPa and its optimal growth pressure is 52 MPa. In order to study the mechanisms used by this species to grow under high pressures, transcriptomics (DNA chips) and proteomics (LC-MS/MS) experiments have been carried out at different pressures, notably sub- and...
Tipo: Text Palavras-chave: Micro-organismes; Pression; Piézophile extrême; Hyperthermophile; Microorganisms; Hyperthermophilic.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00609/72138/70890.pdf
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Chitin/toxicant interactions and microbial degradation within estuarine ecosystems. ArchiMer
Portier, R; Meyers, S.
The environmental effect and fate of several agricultural and industrial source toxicants were evaluated in controlled laboratory microcosm systems in the presence of the aminopolysaccharide polymer, chitin. Toxicants examined included methyl parathion, phenol, pentachlorophenol, 2,4 dichlorophenol, 1,2 dichloroethane and Kepone. Microcosm operating parameters, including pH/Eh, salinity, temperature, flow rate and sediment/water interface conditions, were based on comparable in situ measurements. Fate analysis predictions were based on mineralization of the parent toxicant compound as measured by chromatographic analysis or super(14)CO sub(2) expiration. Chitin, amended to continuous flow microcosms, promoted either cometabolic or cooxidative...
Tipo: Text Palavras-chave: Controlled conditions; Chemical pollutants; Industrial wastes; Pesticides; Metabolism; Food webs; Biodegradation; Ecosystems; Microorganisms; Environmental effects; Brackishwater pollution; Estuaries; Toxicants; Chitin.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1011.pdf
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Role of bacteria in the gastral cavity of Anthozoa. ArchiMer
Herndl, G; Velimirov, B.
Bacterial density of the coelenteric fluid (CF) of some anthozoan species was determined in situ and under laboratory conditions. In all species examined bacterial density of the CF was higher in the gastral cavity than in ambient ranging from 10 super(5) up to 5 x 10 super(6) cells. ml super(-1). Incubation experiments with the CF indicate that a bacterial population within the coelenteron is efficiently controlled by the CF, favoring bacterial growth if bacterial densities are low in the CF and showing clearance activity at high bacterial densities. Feeding of pre-starved anthozoans caused a rapid response of coelenteric rod-shaped bacteria. Evidence is presented that coelenteric bacteria are digested periodically although they contribute 1% to the...
Tipo: Text Palavras-chave: Anthozoa; Coelomic fluids; Bacteria; Microorganisms; Interspecific relationships; Metabolism; Nutrition.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-989.pdf
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Etude ecologique des vibrions du bassin d'Arcachon ArchiMer
Marchand, Michel.
Water and sediment samples monthly collected from Arcachon Bay (Gironde, France) were examined during three years for the presence of marine vibrios. V. cholerae serotype 01 was never detected. On the other hand, the Eyre River and mouth contain non-agglutinating V. cholerae (127 strains) principally in water. The low temperatures slow their development down without stopping it completely. V. parahaemolyticus (257 strains) present in the same areas is particularly isolated from water when the aquatic medium temperature rises to 15 degree C. V. alginolyticus (638 strains) settles the whole Bay, except the river, in any season. The low temperatures promote growth of V. metschnikovii (203 strains) very tolerant towards salinity. V. anguillarum (46 strains)...
Tipo: Text Palavras-chave: Vibrio metschnikovii; Vibrio anguillarum; Vibrio alginolyticus; Vibrio cholerae; Vibrio; Marine environment; Microorganisms; Sediments; Salinity effects; Temperature effects; Ecology; Water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-998.pdf
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Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system. ArchiMer
Velimirov, B; Herndl, G; Kavka, G.
The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...
Tipo: Text Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf
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Polychaete tube walls as zonated microhabitats for marine bacteria. ArchiMer
Reichardt, W.
The predominantly anoxic, sulfide-rich sandy sediment of a shallow lagoon at Kiel Fjord was densely inhabited by the polychaete worm Nereis diversicolor (280 cm super(3) m super(-2) of biomass volume). Burrow walls which contrasted by their brown coloration of at least 1.5 mm thickness with the bulk of the reduced black sediment, made up for 6% of the total volume of the 10 cm cores investigated. Only the uppermost mm of the internal surface layer contained detectable O sub(2). As a result of the build up of internal redox gradients, CO sub(2) dark fixation was activated in the burrow walls (by a factor of approximately equals 2). Assays of ribulose-biphosphate carboxylase activities and viable counts of potentially chemoautotrophic bacteria in different...
Tipo: Text Palavras-chave: Nereis diversicolor; Polychaeta; Bacteria; Microorganisms; Carbon cycle; Tube dwellers; Carbon fixation; Burrows; Bioturbation; Sediments.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-990.pdf
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Sequential resuspension of biofilm components (viruses, prokaryotes and protists) as measured by erodimetry experiments in the Brouage mudflat (French Atlantic coast) ArchiMer
Dupuy, Christine; Mallet, Clarisse; Guizien, Katell; Montanie, Helene; Breret, Martine; Mornet, Francoise; Fontaine, Camille; Nerot, Caroline; Orvain, Francis.
Resuspension thresholds in terms of friction velocity were experimentally quantified for the prokaryotes, protists and for the first time, viruses of intertidal mudflat biofilms. Differences in resuspension thresholds could be related to the type, behaviour and size of microorganisms and their association with particles. Free microorganisms (viruses, bacteria and some nanoflagellates) were resuspended by weak flow at friction velocities lower than 2 cm s- 1. Chlorophyll a, some nanoflagellates and attached bacteria were resuspended together with the bed’s muddy sediment, which required friction velocities larger than 3 cm s- 1. Diatoms smaller than 60 μm were resuspended at velocities between 3 and 5 cm s- 1, while those larger than 60 μm were resuspended...
Tipo: Text Palavras-chave: Viruses; Microorganisms; Resuspension; Benthic-pelagic coupling; Spatial distribution; Mudflat.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00168/27922/26196.pdf
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Biodegradation du petrole en mer. Influence de l'apport d'azote et de phosphore sous forme minerale ArchiMer
Lepetit, J; Guiot, J; Martin, Y; Tagger, S.
To exploit the presence in the sea of microorganisms capable of degrading petroleum products a mineral fertilizer, containing immediatly assimilable nitrogen and phosphorus compounds, was used to increase the microbial population and therefore, the degradation potential of treated polluted waters. The fertilizer supply caused an increase in heterotrophic and hydrocarbon-splitting bacteria and an increase in the phytoplanktonic biomass. The latter is characterized by an increase in a few selected species; it constitutes an important supply of organic substances, and it is responsible for increasing the dissolved oxygen which is essential for petroleum oxidation. Supplying the fertilizer results in an increase in the treated water degradation potential and a...
Tipo: Text Palavras-chave: Bacteria; Microorganisms; Heterotrophic organisms; Phosphorus compounds; Nitrogen compounds; Fertilizers; Hydrocarbons; Biodegradation; Sea water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1020.pdf
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Microbiological analysis of water used in hydrotherapy J. Venom. Anim. Toxins incl. Trop. Dis.
Perestrelo,M. F.; Norberg,A. N.; Guerra-Sanches,F.; Torres,A. C.; Pile,E..
Water used in hydrotherapy units of Nova Iguaçu and Nilópolis, Rio de Janeiro State, Brazil, was microbiologically analyzed. Thirty samples (5ml each) were weekly collected from September 2001 to June 2002 before the beginning and after the end of activities in the units. For analysis, routine techniques were used, which showed the presence of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp, Candida spp, Penicillium spp, Epidermophyton spp, Aspergillus fumigatus, Aspergillus niger, Aspergillus spp, Cephalosporium spp, Cladosporium spp, Trichophyton rubrum, and Trichophyton spp. Results indicated a need for improving hygienic conditions, suggesting that water might be a contamination source in the evaluated units.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrotherapy; Water; Microorganisms; Bacteria; Fungi.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300006
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Estudios de enterobacterias en las inmediaciones de Punta Fort William, Isla Greenwich OceanDocs
Helguero, C.; Valencia T., M..
Se presenta un estudio microbiológico de las aguas de Ensenada Guayaquil y Bahía Chile, con el propósito de determinar presencia de enterobacterias en las mismas; se detectó la existencia de tres grupos: Escherichiae, Yersinieae y Klebsielleae. Se estima que eventualmente dicha presencia es ocasionada por la presencia de la actividad humana en el área y por el aporte de los excrementos de las aves transportadas en aguas provenientes de deshielos en el verano.
Tipo: Journal Contribution Palavras-chave: Microbiological analysis; Microorganisms; Polar waters; Microbiological analysis; Microorganisms; Http://aims.fao.org/aos/agrovoc/c_25302; Http://aims.fao.org/aos/agrovoc/c_4807.
Ano: 1993 URL: http://hdl.handle.net/1834/3808
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Macrofauna bentónica en la zona intermareal y submareal en Caleta Aeolian, Isla Baltra (Galápagos, Ecuador) OceanDocs
Villamar, F.; Cruz, M..
Esta investigación tiene como propósito identificar y dar a conocer las especies de marco invertebrados bentónicos más comunes del área de la Caleta de Aeolian, Isla Baltra del Archipiélago de Galápagos, y conocer la actividad biológica de estos organismos marinos en la zona intermareal y submareal con la finalidad de determinar la composición, distribución y abundancia de cada uno de ellos y sus asociaciones existentes en la zona estudiada, como un marco referencial futuro que nos permita conocer los cambios para evaluar el impacto ambiental que puedan tener los hábitat de estos invertebrados marinos.
Tipo: Journal Contribution Palavras-chave: Microorganisms; Abundance; Marine organisms; Community composition; Distribution; Marine invertebrates; Benthos; Intertidal environment; Distribution; Benthos; Intertidal environment; Microorganisms; Http://aims.fao.org/aos/agrovoc/c_35773; Http://aims.fao.org/aos/agrovoc/c_878; Http://aims.fao.org/aos/agrovoc/c_3923; Http://aims.fao.org/aos/agrovoc/c_4807.
Ano: 2007 URL: http://hdl.handle.net/1834/2405
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Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements OceanDocs
This manual describes the protocol approved by the international Scientific Steering Committee for the Joint Global Ocean Flux Study (JGOFS) for the most of the 20 JGOFS Core Measurements. However, the methods for the analysis of various parameters of the seawater CO2 system are described in a separate handbook. In order to have a complete set of the JGOFS measurements protocols, you should request a copy of the "Handbook of Methods for the Analysis of the Various Parameters of the Carbon Dioxide System in Seawater"version 2, A.G. Dickson and C. Goyet, eds. ORNL/CDIAC-74.
Tipo: Book Palavras-chave: Oceanography; Ocean circulation; Oceanographic stations; Oceanographic data; Oceanographic surveys; Samples; Sampling; Data processing; Data; Data collections; Quality control; Salinity measurement; Oxygen; Inorganic carbon; Nitrites; Nitrates; Phosphorus compounds; Phosphorus cycle; Silicate minerals; Marine organisms; Microorganisms; Bacteriology; Bacteria; Zooplankton; Sampling; Nitrites; Nitrates; Zooplankton; Data processing; Data; Oxygen; Microorganisms; Bacteriology; Bacteria; Http://aims.fao.org/aos/agrovoc/c_6774; Http://aims.fao.org/aos/agrovoc/c_5190; Http://aims.fao.org/aos/agrovoc/c_5187; Http://aims.fao.org/aos/agrovoc/c_15490; Http://aims.fao.org/aos/agrovoc/c_10289; Http://aims.fao.org/aos/agrovoc/c_49816; Http://aims.fao.org/aos/agrovoc/c_5477; Http://aims.fao.org/aos/agrovoc/c_4807; Http://aims.fao.org/aos/agrovoc/c_768; Http://aims.fao.org/aos/agrovoc/c_765.
Ano: 1994 URL: http://hdl.handle.net/1834/2795
Registros recuperados: 91
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