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| Registros recuperados: 81 | |
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| Oliveira-Klein,Mariana de; Cardoso-dos-Santos,Augusto César; Tagliani-Ribeiro,Alice; Fagundes,Nelson Rosa; Matte,Ursula; Schuler-Faccini,Lavinia. |
| Abstract Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insulin-like growth factor; Founder effect; Microsatellite; Reproduction; Twinning. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500775 |
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| Barbosa,Anna C.D.R.; Galzerani,Felipe; Corrêa,Thaís C.; Galetti Jr.,Pedro M.; Hatanaka,Terumi. |
| Prochilodus is one of the most important fish resources of South America, in addition to the important role it plays in nutrient cycling of Neotropical rivers. In the present study, we describe the isolation and characterization of nine novel microsatellite loci in Prochilodus argenteus. The number of alleles per polymorphic locus varied from 5 (Par76) to 21 (Par85), revealing a total of 116 alleles. The values of observed and expected heterozygosities ranged from 0.629 (Par69) to 0.926 (Par85 and Par86) and from 0.643 (Par66) to 0.931 (Par80), respectively. Furthermore, the ability of these and other previously described microsatellite markers to amplify orthologous loci was tested in two related species, Prochilodus costatus and Prochilodus lineatus.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cross-species amplification; Enrichment libraries; Microsatellite; Prochilodus. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200032 |
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| Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli. |
| The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010 |
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| Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.. |
| Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014 |
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| Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui. |
| This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013 |
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| Mignotte, Antoine; Garros, Claire; Dellicour, Simon; Jacquot, Maude; Gilbert, Marius; Gardès, Laetitia; Balenghien, Thomas; Duhayon, Maxime; Rakotoarivony, Ignace; De Wavrechin, Maïa; Huber, Karine. |
| Background In the last two decades, recurrent epizootics of bluetongue virus and Schmallenberg virus have been reported in the western Palearctic region. These viruses affect domestic cattle, sheep, goats and wild ruminants and are transmitted by native hematophagous midges of the genus Culicoides (Diptera: Ceratopogonidae). Culicoides dispersal is known to be stratified, i.e. due to a combination of dispersal processes occurring actively at short distances and passively or semi-actively at long distances, allowing individuals to jump hundreds of kilometers. Methods Here, we aim to identify the environmental factors that promote or limit gene flow of Culicoides obsoletus, an abundant and widespread vector species in Europe, using an innovative framework... |
| Tipo: Text |
Palavras-chave: Culicoides obsoletus; Landscape genetics; Microsatellite; Dispersal; Palearctic region. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00678/79022/81428.pdf |
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| Muths, Delphine; Bourjea, Jerome. |
| Epinephelus merra (Serranidae) is the most common and widespread species of this genus, mostly found in reef habitats from South Africa to Central Pacific. Thirteen polymorphic microsatellite loci were isolated and characterized from this species. Polymorphism was studied in two localities from the Comoros archipelago-Moh,li and Moroni. Allelic diversity was 7.5, polymorphism ranging from 3 to 13 alleles. Overall heterozygosity was high with an average observed heterozygosity of 0.587. Three of these loci were characterised by heterozygote deficiencies for both populations. Finally, ten of the 13 developed primers could be used in future population genetics study of E.merra that might be useful in a context of marine biodiversity conservation. |
| Tipo: Text |
Palavras-chave: Microsatellite; Epinephelus merra; Reef fish; Indian ocean. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00044/15529/12957.pdf |
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| Muths, Delphine; Tessier, Emmanuel; Bourjea, Jerome. |
| The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally. Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population-level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the... |
| Tipo: Text |
Palavras-chave: Cytochrome b; Marine connectivity; Microsatellite; Reef fish; West Indian Ocean. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00189/30046/28566.pdf |
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| Hervouet, Veronique. |
| Les marqueurs génétiques très polymorphes sont des outils particulièrement intéressants pour l'analyse de contributions parentales. Dans notre étude, nous avons utilisé un marqueur microsatellite pour analyser les contributions parentales dans deux croisements contrôlés chez l'huître creuse Crassostrea gigas. 5 mâles et 5 femelles, hétérozygotes à ce locus, et portant chacun des allèles différents (soit 20 allèles ségrégés au total) ont été croisés de deux manières différents: soit en mélangeant toutes les gamètes mâles d'une part, toutes les gamètes femelles d'autre part, et en réalisant un croisement entre ces deux mélanges; soit en fécondant séparément chacune des 5 femelles par chacun de 5 mâles, soit 25 croisement séparés. Pour le deuxième type de... |
| Tipo: Text |
Palavras-chave: Contributions parentales; Compétition; Microsatellite; Crassostrea gigas. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00032/14372/11661.pdf |
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| Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre. |
| Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The... |
| Tipo: Text |
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois. |
| Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea... |
| Tipo: Text |
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf |
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| Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie. |
| Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe during the XVIth and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI). This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier... |
| Tipo: Text |
Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf |
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| Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),... |
| Tipo: Text |
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf |
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| Registros recuperados: 81 | |
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