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Registros recuperados: 22 | |
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Orzil,Lívia de Lima; Preis,Ingred Sales; Almeida,Iassudara Garcia de; Souza,Patrícia Gomes de; Soares Filho,Paulo Martins; Jacinto,Fabrício Barcelos; Fonseca Júnior,Antônio Augusto. |
ABSTRACT: A multiplex PCR technique for detection of Brucella spp. in samples of bacterial suspension was validated as a complementary tool in the diagnosis of the disease. This technique allows the characterization of the agent without performing biochemical tests, which greatly reduces the time for a final diagnosis, and provides more security for the analyst by reducing the time of exposure to microorganisms. The validation was performed in accordance with the Manual of Diagnostic Tests from OIE (2008) and following the requirements present in the ABNT NBR ISO/IEC 17025:2005. The mPCR validated in this study identified the different species of Brucella ( Brucella abortus , B. suis , B. ovis e B. melitensis ) of bacterial suspension obtained from the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Molecular identification; Zoonosis; Brucella; Multiplex PCR. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500847 |
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Oliveira,Camilla Martins de; Souza Junior,Ismail Teodoro; Araújo Filho,Jerônimo Vieira de; Freitas,Marcos Augusto de; Rocha,Mara Rúbia da; Petrofeza,Silvana. |
ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Root-knot nematode; Glycine max; Esterase; Molecular identification. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500151 |
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Registros recuperados: 22 | |
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