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Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm ArchiMer
Rivera-ingraham, Georgina A.; Nommick, Aude; Blondeau-bidet, Eva; Ladurner, Peter; Lignot, Jehan-herve.
In the context of global change, there is an urgent need for researchers in conservation physiology to understand the physiological mechanisms leading to the acquisition of stress acclimation phenotypes. Intertidal organisms continuously cope with drastic changes in their environmental conditions, making them outstanding models for the study of physiological acclimation. As the implementation of such processes usually comes at a high bioenergetic cost, a mitochondrial/oxidative stress approach emerges as the most relevant approach when seeking to analyze whole-animal responses. Here we use the intertidal flatworm Macrostomum lignano to analyze the bioenergetics of salinity acclimation and its consequences in terms of reactive oxygen/nitrogen species...
Tipo: Text Palavras-chave: Energetic balance; Flatworms; Live-imaging; Mitochondrial membrane potential; Osmoregulation; RNS; ROS.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00625/73743/74411.pdf
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Digestive tract ontogeny of Dicentrarchus labrax: Implication in osmoregulation ArchiMer
Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R.
The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf
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Acclimation of juvenile Mugil liza Valenciennes, 1836 (Mugiliformes: Mugilidae) to different environmental salinities Neotropical Ichthyology
Lisboa,Viviana; Barcarolli,Indianara F.; Sampaio,Luís A.; Bianchini,Adalto.
Survival and physiological parameters associated with metabolism and osmoregulation were evaluated in juveniles of the Lebranche mullet Mugil liza acclimated to different water salinities (5, 10, 20, 30, and 40‰) for 15 days. Room temperature (25ºC) and photoperiod (12L:12D) were fixed. Fish were fed twice-a-day with commercial diet (28% crude protein) until satiation. After acclimation, whole body oxygen consumption was measured and fish were euthanized and sampled for blood, gills, and liver. Whole body oxygen consumption and plasma osmolality did not change in the range of salinities tested. The isosmotic point was estimated as 412.7 mOsmol kg-1 (13.5‰). Gill Na+,K+-ATPase activity tended to be lower at 20 and 30‰, while liver glycogen content was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Glycogen; Na+; K+-ATPase; Osmoregulation; Oxygen consumption.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300591
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Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction Neotropical Ichthyology
Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A..
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009
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Physiological effects of gasoline on the freshwater fish Prochilodus lineatus(Characiformes: Prochilodontidae) Neotropical Ichthyology
Simonato,Juliana D.; Fernandes,Marisa N.; Martinez,Cláudia B. R..
The purpose of this work was to evaluate the effects of the water-soluble fraction of gasoline (WSFG) on the Neotropical freshwater fish Prochilodus lineatus. The WSFG was prepared by mixing gasoline in water (1:4) and animals were exposed for 6, 24 and 96h to 5% diluted WSFG or only to water. After exposure, blood was collected from the caudal vein and the gills were removed. The following parameters were analyzed: hematological (hemoglobin, hematocrit, number of red blood cells), osmo-ionic (plasma Na+, Cl- and K+ and plasma osmolarity), metabolic (total plasma proteins and glucose), endocrine (cortisol), density and distribution of chloride cells [CC] in the gills (immunohistochemistry), and branchial Na+/K+-ATPase (NKA) activity. Hemolysis was found to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chloride cells; Hematology; Hydrocarbons; Na+K+-ATPase; Osmoregulation; Stress response.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300683
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Plasma ion levels of freshwater and marine/estuarine teleosts from Southern Brazil Neotropical Ichthyology
Becker,Alexssandro Geferson; Gonçalves,Jamile Fabbrin; Toledo,Jaderson dos Anjos; Burns,Marcelo D. M.; Garcia,Luciano de Oliveira; Vieira,João Paes; Baldisserotto,Bernardo.
The purpose of this study was to investigate Na+, Cl-, K+, Ca2+, and Mg2+ levels in the plasma of freshwater and marine/estuarine teleosts collected at different salinities (0 to 34) from the estuarine and freshwater portions of the São Gonçalo channel in Southern Brazil. Any relationship between plasma ion levels and salinity and the capacity of ionic regulation of teleosts found at three or more different salinities (Genidens barbus and Micropogonias furnieri) was also investigated. Results showed no relationship between plasma ion levels and salinity when considering all species together, but the two species collected from three or more different salinities showed a significant positive relationship between plasma ion levels and salinity, indicating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ariidae; Estuaries; Ionoregulation; Osmoregulation; Salinity; Sciaenidae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000400021
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Ion fluxes in silver catfish (Rhamdia quelen) juveniles exposed to different dissolved oxygen levels Neotropical Ichthyology
Rosso,Felipe Link de; Bolner,Keidi C. S.; Baldisserotto,Bernardo.
Low dissolved oxygen levels in the water (hypoxia) can be provoked by oxygen consumption by fish and other organisms, organic matter decomposition, phytoplankton blooms, and temperature increase. The objective of the present study was to investigate Na+, Cl-, K+, and ammonia fluxes in silver catfish (Rhamdia quelen) exposed to different dissolved oxygen levels. Juveniles (9 ± 1g) maintained at 6.0 mg.L-1 dissolved oxygen were transferred to four 40 L aquaria with different dissolved oxygen levels (in mg.L-1): 6.0, 4.5, 3.5, and 2.5. In another series of experiments, juveniles were acclimated at 6.0 or 2.5 mg.L-1 dissolved oxygen levels, and then placed in two 40 L aquaria with 6.0 mg.L-1 dissolved oxygen. For both series of experiments, 1, 24, 48 or 120 h...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxygen saturation; Osmoregulation; Jundiá.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000400007
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The effects of low salinity on lipid composition in the gills of grey mullet "Liza ramada" OceanDocs
Rabeh, I.; Telahigue, K.; Chetoui, I.; Masmoudi, W.; El Cafsi, M..
Les alevins de Liza ramada adaptés à l’eau de mer (EM, 35ppt) ont subi au laboratoire un stress halin par transfert direct de l’eau de mer à l’eau douce (ED, 0,5 ppt) pendant 24 heures. Les aspects hématologiques ainsi que la composition lipidique du tissu branchial des alevins ont été suivis. Le transfert à la basse salinité s’est accompagné d’une diminution significative de l’osmolalité du plasma, du taux d’hématocrite et du nombre des globules rouges. Le suivi de l’évolution des lipides des branchies des alevins en fonction de la salinité du milieu, montre une augmentation des acides gras monoinsaturés, particulièrement l’acide oleique C18 :1(n-9) à la basse salinité. Une réduction des pourcentages des acides gras polyinsaturés de la famille...
Tipo: Journal Contribution Palavras-chave: Osmoregulation; Mullet fisheries; Osmotic adaptations.
Ano: 2010 URL: http://hdl.handle.net/1834/4701
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Physiological responses of two ecologically important Kenyan mangrove crabs exposed to altered salinity regimes OceanDocs
De Wachter, B.; Gillikin, D.P.; Tack, J..
The potential long-term effects of altered salinity regimes on the bioenergetics of two ecologically important Kenyan mangrove crabs, Neosarmatium meinerti de Man, 1887 and Neosarmatium smithi H. Milne Edwards, 1853 were investigated in light of recent findings suggesting that groundwater redirection may alter salinity regimes in Kenyan mangroves. Although changes in groundwater may cause only small increases in salinities, these changes would be chronic and may impact crab populations already living above their optimal salinity. To assess potential impacts, fundamental physiological processes and hemolymph components were measured on animals acclimated to 16‰, 32‰, 48‰ and 65‰ for 4 weeks in a field laboratory. For comparative purposes, crabs were also...
Tipo: Journal Contribution Palavras-chave: Ecophysiology; Salinity Tolerance; Energy Budget; Osmoregulation; Sesarmidae; Crab fisheries.
Ano: 2004 URL: http://hdl.handle.net/1834/693
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Salt concentrations in culture media for the development of Dipteryx alata in vitro PAB
Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de.
Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige & Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295
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An economic evaluation of the osmoregulation gene technology to the Australian wheat industry AgEcon
Farquharson, Robert J.; Morgan, J.M.; Brennan, John P..
Episodes of rainfall irregularity and soil moisture deficit have focused attention on the widespread limitation of water supply on winter cereal crop production in Australia. This has motivated a number of efforts at breeding for improved drought tolerance. A recent example involves a cellular adaptation which mitigates water loss through solute accumulation (osmoregulation or osmotic adjustment). An assessment of the performance of osmoregulation yield response in the presence of climate change found that wheat cultivars with this gene are unlikely to be adversely affected by hotter and drier conditions across the wheat belt. The results of an economic evaluation of potential future innovations (wheat cultivars) from the osmoregulation gene technology are...
Tipo: Conference Paper or Presentation Palavras-chave: Economic evaluation; Wheat breeding; Osmoregulation; Crop Production/Industries; Research Methods/ Statistical Methods.
Ano: 2004 URL: http://purl.umn.edu/58446
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Growth and production components of West Indian cherry cultivated with saline waters and potassium fertilization AGRIAMBI
Lima,Geovani S. de; Pinheiro,Francisco W. A.; Dias,Adaan S.; Gheyi,Hans R.; Soares,Lauriane A. dos A.; Silva,Saulo S. da.
ABSTRACT Due to water limitations in terms of both quantity and quality in the semi-arid region of northeastern Brazil, the use of waters with high concentrations of salts become necessary in irrigated agriculture. Thus, this study aimed to evaluate the growth and production of grafted West Indian cherry under saline water irrigation and potassium fertilization. The experiment was carried out in drainage lysimeters under protected environment conditions, using a Regolithic Neosol with sandy loam texture. Treatments resulted from the combination of two levels of electrical conductivity - ECw (0.8 and 3.8 dS m-1) of irrigation water and four doses of potassium (50, 75, 100 and 125% of the recommendation of Musser), arranged in a randomized block design, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malpighia emarginata; Salt stress; Osmoregulation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662019000400250
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Proline and trehalose in maize seeds germinating under low osmotic potentials AGRIAMBI
Queiroz,Rafaela J. B.; Cazetta,Jairo O..
ABSTRACT Although it is relatively well known that adult plants tend to accumulate proline and trehalose in their tissues as a physiological mechanism in response to drought, there is scarce information about the development of this physiological response in seeds. Thus, the objective of this research was to verify if maize seeds are able to develop mechanism of osmoprotection, when are germinating under low osmotic potential, and the possibility to use the levels of trehalose and proline in a defined seed part, aiming to differentiate genotypes regarding drought tolerance. The experiment was performed as a factorial arrangement of 2 x 5 (2 hybrids x 5 osmotic potential) within a completely randomized design, with four replicates. It was found that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Germination; Water restriction; Osmoregulation; Compatible solutes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000100022
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Oxygen consumption remains stable while ammonia excretion is reduced upon short time exposure to high salinity in Macrobrachium acanthurus (Caridae: Palaemonidae), a recent freshwater colonizer Rev. Bras. Zool.
Freire,Carolina A.; Rios,Leonardo de P.; Giareta,Eloísa P.; Castellano,Giovanna C..
ABSTRACT Palaemonid shrimps occur in the tropical and temperate regions of South America and the Indo-Pacific, in brackish/freshwater habitats, and marine coastal areas. They form a clade that recently (i.e., ~30 mya) invaded freshwater, and one included genus, Macrobrachium Bate, 1868, is especially successful in limnic habitats. Adult Macrobrachium acanthurus (Wiegmann, 1836) dwell in coastal freshwaters, have diadromous habit, and need brackish water to develop. Thus, they are widely recognized as euryhaline. Here we test how this species responds to a short-term exposure to increased salinity. We hypothesized that abrupt exposure to high salinity would result in reduced gill ventilation/perfusion and decreased oxygen consumption. Shrimps were subjected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonia; Lactate; Osmoregulation; Palaemonidae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100323
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Water economy of three Cinclodes (Furnariidae) species inhabiting marine and freshwater ecosystems RChHN
SABAT,PABLO; NESPOLO,ROBERTO F; BOZINOVIC,FRANCISCO.
Birds living in desert environments have been the preferred models for the study of physiological adaptations to water scarcity. Passerine birds living in marine coastal habitats face similar problems, yet physiological adaptations to water conservation in such species have been poorly documented. We measured total evaporative water loss (TEWL) and rates of oxygen consumption (VO2) in three species of passerine birds dwelling in marine and fresh water habitats. Mass specific total evaporative water loss was significantly lower in the marine species, Cinclodes nigrofumosus, than in species inhabiting areas near freshwater sources. We found a positive relationship between TEWL and VO2. The ratio of TEWL to VO2 (relative evaporative water loss, RTEWL) showed...
Tipo: Journal article Palavras-chave: Evaporative water loss; Cinclodes; Osmoregulation; Passerines; Salt.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2004000200001
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Terrestrial birds living on marine environments: does dietary composition of Cinclodes nigrofumosus (Passeriformes: Furnariidae) predict their osmotic load? RChHN
SABAT,PABLO; FARIÑA,JOSÉ M.; SOTO-GAMBOA,MAURICIO.
Feeding on saline marine foods may be especially challenging for passerine birds that lack functional salt glands and have a limited ability to concentrate urine. To reduce the salt load imposed by consumption of marine food these birds may select food with low salt contents and/or increase their intake of freshwater. The genus Cinclodes is particular among passerines because it includes species that inhabit both inland and maritime shores. We analyzed the diet of Cinclodes nigrofumosus and explored the possible relationships between dietary composition and salt load at mesic and arid coastal sites of Chile. From a biogeographical perspective, we hypothesized that freshwater availability is critical to the relationship between diet composition and osmotic...
Tipo: Journal article Palavras-chave: Cinclodes; Dietary habits; Osmoregulation; Osmotic load.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2003000200017
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Osmoregulatory responses to dietary protein and water intake in the granivorous Zonotrichia capensis (Passerine, Emberizidae) RChHN
ALDEA,PATRICIA; SABAT,PABLO.
We studied the osmoregulatory responses of the granivore Zonotrichia capensis, acclimated to high- and low-protein diets as well as two levels of water intake. We tested whether Z. capensis has the ability to modify the proportion of nitrogenous waste in the excreta when protein intake and water intake varies. After 21 days of acclimation, plasma osmolality was not significantly affected by dietary treatment; however, excreta osmolality was higher in the high-protein group compared to the low-protein group. Nitrogenous wastes in Z. capensis are excreted mainly in the form of uric acid. The proportion of kidney devoted to medullary tissue was 40 % higher in dehydrated birds than in hydrated birds. Excreta osmolality was higher in dehydrated birds, and in...
Tipo: Journal article Palavras-chave: Granivory; Kidney; Nitrogenous waste; Osmoregulation; Passerines.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000400006
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Seasonal changes in the use of marine food resources by Cinclodes nigrofumosus (furnariidae, aves): carbon isotopes and osmoregulatory physiology RChHN
SABAT,PABLO; MARTÍNEZ DEL RÍO,CARLOS.
Passerines lack functional salt glands and to a large extent avoid feeding on marine invertebrates. An exception is Cinclodes nigrofumosus. Previous studies reported that the contents of its gastrointestinal tract had a lower osmolality than seawater suggesting that birds were supplementing their marine invertebrate diet with terrestrial invertebrates and fresh water at certain periods of the year. We report seasonal changes in the diet of C. nigrofumosus at two contrasting coastal localities in Central Chile and document the potential correlation between weather and feeding habits with osmoregulatory features in this species. We expected that C. nigrofumosus would increase its use of osmotically challenging marine dietary sources when fresh water is...
Tipo: Journal article Palavras-chave: Cinclodes; Isotopes; Renal function; Osmoregulation; Seasonal changes.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2005000200009
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Evaporative water loss and dehydration during the night in hummingbirds RChHN
HARTMAN BAKKEN,BRADLEY; SABAT,PABLO.
Nectar-feeding birds oscillate between avoiding overhydration when they are feeding and preventing dehydration during fasts. Here, we examined how resting rates of total evaporative water loss (TEWL) and metabolic water production (MWP) influence water balance in the green-backed firecrown (Sephanoides sephanoides), a Chilean hummingbird. We hypothesized that a circadian rhythm in TEWL would assuage the dehydration risk that hummingbirds face during the night. However, we did not find support for this idea. In resting hummingbirds, rates of TEWL during the day (54 ±6 µLh-1,n = 8) and night (65 ± 12 h-1, n = 5) were similar. Rates of MWP were also similar between the day (22 ±3 µL h-1, n = 8) and night (23 ± 2 µL h-1, n = 5). MWP rates were significantly...
Tipo: Journal article Palavras-chave: Circadian rhythm; Evaporative water loss; Hummingbird; Osmoregulation; Water balance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000300001
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Accumulation of organic and inorganic solutes in NaCl-stressed sorghum seedlings from aged and primed seeds Scientia Agricola
Oliveira,Alexandre Bosco de; Alencar,Nara Lídia Mendes; Prisco,José Tarquinio; Gomes-Filho,Enéas.
Although it has been known that the seed's physiological potential affects its response to osmoconditioning and abiotic stresses, researches involving seed aging and priming associated to abiotic stresses are scarce. The aim of this work was to evaluate the role of seed priming on salt tolerance in sorghum seedlings from seeds with two vigor levels (aged or non-aged) and to verify the organic and inorganic solute contributions as osmoregulators in NaCl-stressed seedlings from aged and primed seeds. The combinations of two seed vigor levels (aged or not), two seed types (primed or not) and two salinity levels (exposed to NaCl at 100 mM or not) were evaluated. In low physiological quality seeds (aged seeds), priming provided an attenuation of salinity's...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorghum bicolor; Accelerated aging; Priming; Salinity; Osmoregulation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600004
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