|
|
|
Registros recuperados: 46 | |
|
|
Krapp-Schickel, T.; Koenemann, S.. |
The amphipod family Stenothoidae contains more than 200 species in about 40 genera; these genera are at present often defined not by the presence, but by the absence of synapomorphies, thus defining grades rather than clades. Our phylogenetic analyses yielded 4 groups of stenothoids: a basic proboloidid clade; an advanced and always clearly separated Austral-Antarctic thaumatelsonid clade, with a possibly related Arctic mesometopid clade; and finally a poorly resolved group, the stenothoids sensu stricto, including the large and probably polyphyletic genera Stenothoe and Metopa, each with more than 50 species. It is proposed to study and analyse these groups separately in future, based on better redescriptions of the individual species. Our analyses... |
Tipo: Article / Letter to the editor |
Palavras-chave: Taxonomy; Amphipoda; Stenothoidae; Thaumatelsonidae; Phylogenetic analysis; 42.74. |
Ano: 2006 |
URL: http://www.repository.naturalis.nl/record/214545 |
| |
|
|
Jaklitsch, W.M.; Samuels, G.J.; Ismaiel, A.; Voglmayr, H.. |
Trichoderma viridescens is recognised as a species complex. Multigene analyses based on the translation elongation factor 1-alpha encoding gene (tef1), a part of the rpb2 gene, encoding the second largest RNA polymerase subunit and the larger subunit of ATP citrate lyase (acl1) reveals 13 phylogenetic species with little or no phenotypic differentiation. This is the first use of acl1 in Trichoderma phylogenetics. The typification of T. viridescens s.str. is clarified and Hypocrea viridescens is replaced by the new name T. paraviridescens. Besides these two species, eleven are phylogenetically recognised and T. olivascens, T. viridarium, T. virilente, T. trixiae, T. viridialbum, T. appalachiense, T. neosinense, T. composticola, T. nothescens and T.... |
Tipo: Article / Letter to the editor |
Palavras-chave: Acl1; Hypocrea; Hypocreaceae; Phylogenetic analysis; Rpb2; Systematics; Tef1. |
Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/532266 |
| |
|
| |
|
|
Londoño-Burbano,Alejandro; Reis,Roberto E.. |
ABSTRACT A taxonomic revision and phylogenetic analysis were completed for Dasyloricaria . The genus includes three valid species: D . filamentosa and D . latiura previously included in the genus, and a new species described herein. Dasyloricaria have a restricted trans-Andean distribution, with D . filamentosa occurring at the lower and middle Magdalena, lower Cauca, and Sinu in Colombia, and lago Maracaibo basin in Colombia and Venezuela; D . latiura in the Atrato and the Tuyra basins in Colombia and Panama, respectively; and the new species in the upper and middle Magdalena basin in Colombia. New synonyms for D . filamentosa and D . latiura are proposed, and a lectotype is designated for the latter. Dasyloricaria is herein recognized as monophyletic,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Armored catfishes; Identification key; Loricariinae; Northwestern South America; Phylogenetic analysis. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100217 |
| |
|
| |
|
|
Wang,Y; Zhang,H; Gu,C; Cheng,G; Zhang,W; Mehmood,K; Hu,X. |
ABSTRACT Newcastle disease is a highly contagious disease responsible for major outbreaks and considerable economic losses in the poultry industry in China. There is still little information available regarding gene characterization of the NDV, especially in ducks and pigeons. Therefore, the aim of this study was to investigate NDV isolated from ducks and pigeons in Hubei, China. In this study, three NDVs from ducks and pigeons were isolated between 2013 and 2015.The fusion protein (F) gene of the NDV isolates was sequenced and phylogenetically analyzed. The clinical signs and gross histopathological lesions were examined. Phylogenetic analysis of these strains indicated that all the sequences are classified as genotype II. The isolates shared a 112... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle disease virus; Phylogenetic analysis; Pathology; Ducks; Pigeons.. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100145 |
| |
|
|
Aşkar,S. |
ABSTRACT Chicken infectious anemia (CIA) is an immune-suppressive disease caused by chicken anemia virus (CAV). It is characterized by lymphoid atrophy, aplastic anemia, especially in chicks. In this study, full-length genomic characterization of CAV DNA from the broiler flocks in Turkey and phylogenetic analysis were aimed. In the study, CAV DNA were found positive for 37 (53%) flocks with PCR studies from thymus tissues of each 70 broiler flocks. And 17 purified CAV DNA PCR products from these 37 CAV isolates were full length sequenced with the NGS method (Illumina MiSeq). Also with the phylogenetic analyses, full length PCR products of 17 purified CAV isolates have been determined as 2298bp genome size and 99% similarity with each other. The highest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Chicken Infectious Anemia; Phylogenetic analysis. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300323 |
| |
|
| |
|
|
Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi. |
Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013 |
| |
|
|
Jia,Rui; Nie,Liu-Wang; Wang,Ning; Wang,Jingjing. |
The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DEAD-box; Phylogenetic analysis; RT-PCR. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000200014 |
| |
|
| |
|
| |
|
|
García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A. |
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in... |
Tipo: Journal article |
Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006 |
| |
|
|
Kumar,Pradeep; Kamle,Madhu; Misra,Asok Kumar; O'Donovan,Anthonia; Pagano,Marcela; Modi,Dinesh Raj. |
ABSTRACT Fusarium mangiferae (=F. subglutinans) isolates collect from malformed samples from major mango-growing area of North India. Molecular identification and characterization of eleven most virulent isolates of F. mangiferae, based on pathogenicity tests used for the present study. Species-specific, genus specific ITS-PCR and PCR-RFLP performed for the accurate and easy detection of F. mangiferae. The rDNA-ITS 28S region sequences used for phylogenetic analysis of Fusarium isolates from India and other countries for homology search between them. The phylogenetic tree divided the isolates into three clades (i.e., American, Asian and African) and showed the high level of sequence based similarity (69-99%) among all Fusarium sequences from Asia. Thus,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium mangiferae; Detection; Phylogenetic analysis; Vegetative malformation; Floral malformation.. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100204 |
| |
|
| |
|
|
Zhao,Ting; Ye,Zhixu; Wang,Binlin; Cui,Yuxia; Nie,Yingjie; Yang,Bin; Chen,Kun; Zhang,Hua; Hu,Fangfang; Yu,Fuxun. |
ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human respiratory syncytial virus; Virus isolation; RT-PCR; HVR2; Phylogenetic analysis; Molecular epidemiology. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600427 |
| |
|
|
Nishiya,Anna Shoko; Almeida-Neto,César de; Romano,Camila Malta; Alencar,Cecília Salete; Ferreira,Suzete Cleusa; Di-Lorenzo-Oliveira,Claudia; Levi,José Eduardo; Salles,Nanci Alves; Mendrone-Junior,Alfredo; Sabino,Ester Cerdeira. |
ABSTRACTBACKGROUND: It is recognized that hepatitis C virus subtypes (1a, 1b, 2a, 2b, 2c and 3a) originated in Africa and Asia and spread worldwide exponentially during the Second World War (1940) through the transfusion of contaminated blood products, invasive medical and dental procedures, and intravenous drug use. The entry of hepatitis C virus subtypes into different regions occurred at distinct times, presenting exponential growth rates of larger or smaller spread. Our study estimated the growth and spread of the most prevalent subtypes currently circulating in São Paulo.METHODS:A total of 465 non-structural region 5B sequences of hepatitis C virus covering a 14-year time-span were used to reconstruct the population history and estimate the population... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth rate; HCV; Phylogenetic analysis; Subtypes. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500473 |
| |
|
|
Médici,K.C.; Barry,A.F.; Alfieri,A.F.; Alfieri,A.A.. |
Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Piglets; Diarrhea; Group B rotavirus; NSP2 gene; Phylogenetic analysis. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100003 |
| |
|
|
Cunha,L.K.H.; Kashima,S.; Amarante,M.F.C.; Haddad,R.; Rodrigues,E.S.; Silva,K.L.T.; Lima,T.A.; Castro,D.B.; Brito,F.C.; Almeida,E.G.; Covas,D.T.; Malheiro,A.. |
Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200003 |
| |
|
| |
Registros recuperados: 46 | |
|
|
|