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| Registros recuperados: 53 | |
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| Peteira-Delgado,B; Hidalgo-Díaz,L. |
| The mode of action of Pochonia as a biological control agent is the penetration by means of the hyphae into the target nematode eggs. The egg shell of nematodes has an outer vitelline membrane composed mainly by proteins. Thus, hyphal penetration is the result of a physic pressure and the specific hydrolytic activity of some enzymes such as proteases and chitinases. The most important protease studied in Pochonia chlamydosporia var. chlamydosporia is VCP1. The aim of this work was to detect and characterize the protease VCP1 in a strain of P. chlamydosporia var. catenulata, a potential biological control agent for root-knot nematodes. The specific activity of this protease was tested on different culture media supplemented with protein inductors using... |
| Tipo: Journal article |
Palavras-chave: Nematode parasites; RFLP; Enzymes; Protease. |
| Ano: 2009 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522009000300006 |
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| Souza,Rocheli de; Schons,Jurema; Brammer,Sandra P.; Prestes,Ariano M.; Scheeren,Pedro L.; Nicolini-Teixeira,Fernanda; Cecchetti,Dileta; Lanzarini,Ariane C.. |
| Com o objetivo de conhecer as alterações metabólicas promovidas pelo Soil-borne wheat mosaic virus (SBWMV), um dos vírus economicamente mais importantes da cultura do trigo (Triticum aestivum), foram analisados os níveis de proteínas solúveis e determinadas as atividades da peroxidase e da protease em quatro cultivares (BRS Guabiju, BRS 194, BRS 179, BR 23) e uma linhagem (PF 980524) de trigo com diferentes níveis de resistência ao vírus. Os dados obtidos foram submetidos à análise de variância, comparando-se as médias, pelo Teste de Duncan a 5%. Os níveis de proteínas solúveis foram mais elevados nas plantas sem sintomas, enquanto que as atividades da peroxidase e da protease foram maiores em plantas com sintoma de mosaico do que em plantas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteínas solúveis; Peroxidase; Protease; Furovirus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400010 |
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| Tremacoldi,Célia R.; Pascholati,Sérgio F.. |
| As raízes de eucalipto (Eucalyptus urophylla) podem estar associadas a fungos como Pisolithus tinctorius, formando uma simbiose conhecida como ectomicorriza, mas também podem estar colonizadas por fungos patogênicos, como Rhizoctonia solani, agente causal do tombamento de plantas em viveiros. O objetivo deste trabalho foi verificar a presença de atividade inibitória de tripsina, uma serino-protease, em raízes de E. urophylla e a atividade de tripsina em filtrados desses fungos. Alíquotas de extrato protéico bruto de raízes de E. urophylla e frações protéicas parcialmente purificadas por cromatografia de exclusão molecular, do tipo Sephacryl S-100-HR, foram testadas para atividade inibitória de tripsina. Proteínas do extrato ou das frações, quando incubadas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inibidor de protease; Protease; Ectomicorriza; Rhizoctonia solani. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200003 |
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| Neves,Kilma Cristiane Silva; Porto,Ana Lúcia Figueiredo; Teixeira,Maria Francisca Simas. |
| O objetivo deste trabalho foi identificar 50 leveduras isoladas a partir de diferentes substratos da Região Amazônica e selecionar uma espécie de maior atividade proteolítica. Entre as 26 espécies identificadas predominaram Candida aquatica (12%) e Candida tropicalis (10%). A fermentação submersa foi realizada em Extrato de Malte suplementado com gelatina 1%, Candida intermedia foi a que expressou maior atividade proteolítica, halo = 25 mm e 273 U/mL, crescimento máximo a 30 ºC, viabilidade celular 6,2 x 10(6) UFC, em 48 horas, não demonstrou características de patogenicidade e a melhor densidade do inóculo foi 3%, utilizando-se culturas com 24 horas de crescimento em ágar Malte. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leveduras; Região Amazônica; Protease. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672006000300002 |
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| Rodarte, Mirian Pereira; Ufla; Dias, Disney Ribeiro; UNILAVRAS - Centro Universitário de Lavras; Vilela, Danielle Marques; Ufla; Schwan, Rosane Freitas; Ufla. |
| One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea Arabica) were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positive isolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH values (5.0, 7.0 and 9.0) at 30 oC. Bacillus megaterium, B. subtilis, Enterobacter agglomerans, Kurthia sp, Pseudomonas paucimobilis and Tatumella ptyseos demonstrated the highest proteolytic activities at pH 9.0. One yeast isolate, Citeromyces matritensis, had a proteolytic activity of 2.40 U at pH 5.0. Aspergillus dimorphicus, A. ochraceus, Fusarium moniliforme, F.... |
| Tipo: Pesquisa de campo; Laboratório |
Palavras-chave: 5.01.02.04-4 microbial enzyme; Protease; Coffee fermentation Microbiologia Agrícola microbial enzyme; Protease; Coffee fermentation. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6734 |
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| Santos,André L.S.; Branquinha,Marta H.; D'Avila-Levy,Claudia M.. |
| Plant and insect trypanosomatids constitute the "lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of Leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosomatidae; Lower trypanosomatids; Gp63; Invertebrate host; Adhesion; Nutrition; Protease. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006 |
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| Santos,Anderson F.; Pires,Fabiano; Jesus,Hugo E.; Santos,André L.S.; Peixoto,Raquel; Rosado,Alexandre S.; D'Avila-Levy,Claudia M.; Branquinha,Marta H.. |
| Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algoriphagus antarcticus; Antarctica; Sporosarcina aquimarina; Protease. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109 |
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| Matias,C.F.Q.; Rocha,J.S.R.; Pompeu,M.A.; Baião,R.C.; Baião,N.C.; Lara,L.J.C.; Clímaco,W.L.S.; Pereira,L.F.P.; Caldas,E.O.; Teixeira,M.P.F.; Cardeal,P.C.. |
| Avaliaram-se os efeitos da protease sobre o coeficiente de metabolizabilidade dos nutrientes em dietas contendo farinha de penas (2,0% na fase inicial e 3,0% na fase de crescimento) para frangos de corte machos, Cobb(r), de um a 32 dias de idade. Foram alojadas 336 aves em gaiolas metálicas equipadas com bandeja para coleta total de excretas. Foi utilizado delineamento inteiramente ao acaso, em arranjo fatorial 2x2 (com e sem adição da enzima protease 0,05% e duas valorizações da matriz nutricional da enzima), sendo sete repetições por tratamento, de 12 aves cada. Observou-se, na fase inicial, maior CMPB (P≤0,05) para as aves que, independentemente da adição de protease, consumiram ração com valorização da matriz nutricional da enzima. Não houve efeito dos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coeficiente de metabolizabilidade; Enzimas exógenas; Frango de corte; Protease. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200492 |
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| Moura,F.A.S.; Dourado,L.R.B.; Farias,L.A.; Lopes,J.B.; Lima,S.B.P.; Fernandes,M.L.. |
| RESUMO Objetivou-se avaliar o efeito de complexos enzimáticos sobre a energia metabolizável e o coeficiente de digestibilidade de nutrientes do milheto para frangos de corte. Quinhentos e setenta e seis frangos machos foram distribuídos em 36 gaiolas, com três tratamentos: T1 - composição de milheto sem complexo enzimático; T2 - composição de milheto com complexo enzimático (CES) e T3 - composição de milheto com complexo enzimático (CEV). Os tratamentos foram definidos com base em seis dietas (três dietas referências e três dietas testes). As dietas testes foram obtidas pela substituição de 40% da dieta referência por milheto inteiro, e a adição de enzimas consistiu de dois complexos enzimáticos: CES, constituído pelas enzimas fitase, protease, xilanase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alimento alternativo; Dietas; Enzimas; Protease; Xilanase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300990 |
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| Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de. |
| The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003 |
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| Varella,Rafael Brandão; Ferreira,Selma Baía; Castro,Márcia Braga de; Tavares,Marisa Dias; Zalis,Mariano Gustavo. |
| We investigated the occurrence of HIV-1 antiretroviral resistance in individuals failing to respond to highly active antiretroviral therapy (HAART) attended by RENAGENO from 2001-2004. One hundred and seventeen patients were selected for this study; their plasma viral RNA was extracted and the PR and RT genes sequenced to examine subtype, genetic polymorphisms and mutations associated with resistance to antiretroviral drugs. HIV-1 sequence analysis showed that 86/100 (86%) were infected with subtype B, 7/100 (7%) with subtype F and 7/100 (7%) with RT/PR hybrid forms (2 D/B, 2 F/B, 2 B/F and 1 D/F). In 14 (12%) of the samples, the subtype was not determined. The prevalence of resistance mutations was high (93.1%), mainly in the RT gene. The most prevalent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; HAART; Reverse transcriptase; Protease; Resistance. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500007 |
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| Liaw,L.; Crawford,H.C.. |
| Cell interactions with extracellular matrices are important to pathological changes that occur during cell transformation and tumorigenesis. Several extracellular matrix proteins including fibronectin, thrombospondin-1, laminin, SPARC, and osteopontin have been suggested to modulate tumor phenotype by affecting cell migration, survival, or angiogenesis. Likewise, proteases including the matrix metalloproteinases (MMPs) are understood to not only facilitate migration of cells by degradation of matrices, but also to affect tumor formation and growth. We have recently demonstrated an in vivo role for the RGD-containing protein, osteopontin, during tumor progression, and found evidence for distinct functions in the host versus the tumor cells. Because of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Matrix metalloproteinase; Osteopontin; Carcinoma; Protease. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700002 |
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| Orlandelli,Ravely Casarotti; Almeida,Tiago Tognolli de; Alberto,Raiani Nascimento; Polonio,Julio Cesar; Azevedo,João Lúcio; Pamphile,João Alencar. |
| Endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. Microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. In the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and Alternaria alternata, Colletotrichum sp., Phyllosticta citricarpa and Moniliophthora perniciosa. We also examined the proteolytic activities of endophytes grown in liquid medium and conducted cup plate assays. The results showed that certain strains in the assemblage of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antagonism; Competitive interaction; Dual culture; Cup plate; Protease. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200359 |
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| Silva,Lidiane Roberta Cruz da; Souza,Odacy Camilo de; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Coelho,Rosalie Reed Rodrigues; Souza-Motta,Cristina Maria. |
| Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shrimp; Fungi; Water; Protease; Aflatoxin B. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100007 |
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| Registros recuperados: 53 | |
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