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Palavra-chave Pseudomonas aeruginosa (66) Biofilm (7) Cystic fibrosis (7) Antimicrobial resistance (4) Acinetobacter baumannii (3) Antibiotic resistance (3) Metallo-beta-lactamase (3) Risk factor (3) Staphylococcus aureus (3) Acinetobacter spp (2) Acquired immunodeficiency syndrome (2) Biofilms (2) Biosurfactant (2) Disease outbreaks (2) Genotyping (2) Imipenem (2) Metallo-β-lactamases (2) Molecular epidemiology (2) Multiresistance (2) Resistance (2) Mais... Tipo do documento Info:eu-repo/semantics/article (59) Info:eu-repo/semantics/other (3) Journal article (2) Text (2) Ano 2020 (1) 2019 (1) 2018 (1) 2017 (3) 2016 (1) 2015 (4) 2014 (8) 2013 (2) 2012 (4) 2011 (9) 2010 (6) 2009 (4) 2008 (6) 2007 (3) 2006 (2) 2005 (2) 2004 (3) 2003 (2) 2002 (2) 2001 (1) Mais... País Brazil (58) Argentina (2) France (2) Chile (1) Colombia (1) Cuba (1) Mexico (1) Mais... Idioma Inglês (59) Espanhol (4) Português (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 66 Primeira ... 1 2 3 4 ... Última Prevalence of carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii in high complexity hospital BJID Baumgart,Ana Milda Karsten; Molinari,Marcelo André; Silveira,Alessandro Conrado de Oliveira. Pseudomonas aeruginosa and Acinetobacter baumannii are Gram-negative bacilli that in the last decades have become prevalent agents of hospital infection due to high antimicrobial resistance developed by these microorganisms. The present study is a retrospective analysis of all positive cultures for these microorganisms in the period of January 2004 to December 2008. Resistance levels of A. baumannii and P. aeruginosa to carbapenems was high and showed a trend to increase during the period of study. In recent years the increasing incidence and resistance levels of A. baumannii and P. aeruginosa to the antimicrobials used for their treatment in the hospital setting underscores the relevance of infections caused by these bacteria. The selective pressure... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Pseudomonas aeruginosa; Drug resistance; Carbapenems; Hospital infection control program. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500002 Factors associated with epidemic multiresistant Pseudomonas aeruginosa infections in a hospital with AIDS-predominant admissions BJID Gomes,Marisa Zenaide Ribeiro; Oliveira,Raquel Vasconcellos C. de; Machado,Carolina Romero; Conceição,Magda de Souza da; Souza,Cristina Vieira de; Lourenço,Maria Cristina da Silva; Asensi,Marise Dutra. INTRODUCTION: Infections caused by multiresistant Pseudomonas aeruginosa (MR-PA) have been associated with persistent infections and high mortality in acquired immunodeficiency syndrome (AIDS) patients. Therefore, understanding the predisposing factors for infection/colonization by this agent is critical for controlling outbreaks caused by MR-PA in settings with AIDS patients. OBJECTIVEAND METHODS: To analyze the presence of factors associated with the acquisition of an epidemic MR-PA strain in a hospital with AIDS-predominant admission. A case-control study was carried out in which cases and controls were gathered from a prospective cohort of all hospitalized patients in an infectious disease hospital during a five-year study period. RESULTS: Multivariate... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Risk factors; Disease outbreaks; Molecular epidemiology; Acquired immunodeficiency syndrome. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300001 Prevalence and antimicrobial susceptibility of respiratory pathogens in patients with cystic fibrosis BJID Paixão,Vilma Almeida; Barros,Tânia Fraga; Mota,Clélia Maria C; Moreira,Tamy Fagundes; Santana,Maria Angélica; Reis,Joice Neves. Respiratory infection is very common in patients suffering from cystic fibrosis (CF). However, the antimicrobial resistance rate of isolates from CF patients is not often documented. In this study, 279 respiratory specimens of 146 patients were prospectively collected from July to December 2006. Microbiological cultures and antimicrobial susceptibility tests of the most frequently isolated bacteria were performed. Sputum and oropharyngeal swabs were processed for culture. During the study period, 50% of the patients harbored Staphylococcus aureus, 35% Pseudomonas aeruginosa, 4.7% Haemophilus influenzae. Methicillin resistant S. aureus (MRSA) were detected in 8 (6%) patients; ESBL and MBL-producing P. aeruginosa were not identified in these patients. The... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cystic fibrosis; Antimicrobial resistance; Pseudomonas aeruginosa; Staphylococcus aureus. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400016 An outbreak of conjunctivitis caused by multiresistant Pseudomonas aeruginosa in a Brazilian Newborn Intensive Care Unit BJID Brito,D. V. D.; Oliveira,E. J.; Matos,C.; Abdallah,V. O.S.; Gontijo Filho,P.P.. We report an outbreak of conjunctivitis due to Pseudomonas aeruginosa involving seven infants admitted in the Neonatal Intensive Care Unit (NICU) of the Uberlândial Federal University Hospital between March and September 2001. Three infants developed systemic complications (01 sepsis and 02 pneumonias). Ten isolates were obtained from conjunctival cultures and all were resistant to ceftazidime and aminoglycosides. Fast identification of the organism and treatment with imipenem were important in containing the outbreak of P. aeruginosa. Tipo: Info:eu-repo/semantics/other Palavras-chave: Pseudomonas aeruginosa; Nosocomial infection; Neonatal unit. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000400001 Risk factors for pan-resistant Pseudomonas aeruginosa bacteremia and the adequacy of antibiotic therapy BJID Tuon,Felipe F.; Gortz,Lucas W.; Rocha,Jaime L.. INTRODUCTION: The aim of this study was to determine risk factors for acquiring carbapenemresistant Pseudomonas aeruginosa bacteremia (CR-PA) and factors associated with in-hospital mortality. METHODS: Seventy-seven cases of bacteremia caused by P. aeruginosa were evaluated in a hospital with high incidence of CR-PA. Clinical and laboratorial factors, and previous use of antibiotics were also evaluated. In one analysis, CR-PA and carbapenem-susceptible P. aeruginosa (CS-PA) bacteremia were compared. A second analysis compared patients who died with survivors. RESULTS: Among 77 P. aeruginosa bacteremia, 29 were caused by CR-PA. Admission to the intensive care unit, higher number of total leukocytes, and previous use of carbapenem were statistically... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Imipenem; Meropenem; Antibiotic resistance; Risk factor. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000400007 Outbreaks, persistence, and high mortality rates of multiresistant Pseudomonas aeruginosa infections in a hospital with AIDS-predominant admissions BJID Gomes,Marisa Zenaide Ribeiro; Machado,Carolina Romero; Conceição,Magda de Souza da; Ortega,Jois Alves; Neves,Sonia Maria Ferraz M; Lourenço,Maria Cristina da Silva; Asensi,Marise Dutra. INTRODUCTION: Authors have reported increased incidence of multiresistant Pseudomonas aeruginosa (MR-PA) infections worldwide over the last decade. Researchers have proposed multifaceted approaches to control MR-PA infections, but none have been reported in the acquired immunodeficiency syndrome (AIDS) setting. OBJECTIVE AND METHODS: Herein we report the impact of a multifaceted intervention for controlling MR-PA over five years in a hospital with AIDS-predominant admissions and describe the clinical characteristics of MR-PA infection in our patient population. The clinical outcomes of infected patients and molecular characteristics of the isolated strains were used as tools for controlling MR-PA infection rates. RESULTS: Significant temporary decrease of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Disease outbreaks; Infection control; Molecular epidemiology; Acquired immunodeficiency syndrome. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400003 Pseudomonas aeruginosa infections: factors relating to mortality with emphasis on resistance pattern and antimicrobial treatment BJID Pinheiro,Millena R.S.; Lacerda,Heloisa R.; Melo,Renata G.L.; Maciel,Mª Amélia. A retrospective case-control study was conducted to investigate the risk factors for death among intensive care unit patients with Pseudomonas aeruginosa infection. Out of 131 patients investigated, 67 (51.1%) died within 30 days of being diagnosed with this infection. The mean duration of hospital stay before this diagnosis was 28.5 ± 26.5 days. No association was found between bacterial resistance and death in this study (multiresistant p= 0.26; panresistant p= 0.42), but the adequacy of the initial treatment was inversely proportional to the degree of resistance. There was a tendency towards greater mortality among patients who received combination therapy (empirical p= 0.09; definitive p= 0.08), despite the greater frequency of appropriate treatment in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Risk factors; Case-control study; Pseudomonas aeruginosa; Death. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600013 Identifying and controlling a multiresistant pseudomonas aeruginosa outbreak in a latin-american cancer centre and its associated risk factors BJID Cortes,Jorge Alberto; Cuervo,Sonia Isabel; Urdaneta,Ana María; Potdevin,Guillermo; Arroyo,Patricia; Bermúdez,Diana; Correa,Adriana; Villegas,María Virginia. Pseudomonas aeruginosa is an important and frightening microorganism for patients suffering from cancer. Multiresistant P. aeruginosa (MRPA) may appear as a consequence of exposure to multiple antibiotics or from a breakdown in infection control practices. This article reports an MRPA outbreak in a cancer treatment centre and the consequent case control study. Mechanical ventilation was identified as being the main risk factor for developing MRPA colonisation or infection; molecular analysis confirmed the outbreak. A multifaceted strategy was adopted, involving reinforcing hand-washing practices, contact isolation, antibiotic restriction and suction devices for mechanically-ventilated patients. MRPA was controlled and the outbreak ended. Such strategy may... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Epidemiology; Multiple drug resistance; Bacteria; Risk factor. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200005 Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem BJID Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis. Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Antibiotic resistance; Imipenem. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001 Carbapenem-resistant Pseudomonas aeruginosa outbreak in an intensive care unit of a teaching hospital BJID Gales,Ana C.; Torres,Patrícia L.; Vilarinho,Dominique S. O.; Melo,Renata S.; Silva,Claudinéia F. L.; Cereda,Rosângela F.. The genetic similarity of carbapenem-resistant Pseudomonas aeruginosa strains isolated in the Hospital Universitário São Francisco, Bragança Paulista, São Paulo, Brasil, was evaluated by pulsed field gel electrophoresis (PFGE). A unique clone was detected among 5 of 7 isolates, suggesting that cross-contamination might have played a role in the spread of carbapenem-resistant P. aeruginosa strains. Interestingly, a similar PFGE pattern was encountered in a P. aeruginosa strain isolated from Hospital São Paulo that was used as a PFGE control. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; PFGE; Carbapenem. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400001 Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil BJID Stehling,Eliana Guedes; Leite,Domingos S; Silveira,Wanderley D. The present study had as objective to evaluate the genotypic diversity and biological characteristics, such as hemolysin, protease, elastase of 56 clinical strains of Pseudomonas aeruginosa isolated from 13 cystic fibrosis (CF) patients attending at the School Hospital of Campinas State University (UNICAMP), Brazil. Genotypic diversity has been determined by Ribotyping (RT) and the pattern of the enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) of each strain. The production of elastase was significantly different only among mucoid and nonmucoid isolates. Joint results obtained by (RT) and ERIC-PCR methods were able to discriminate all strains isolated from both the same and different patients. Additionally, we observed four strain clusters... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cystic fibrosis; Pseudomonas aeruginosa; Clonal analysis; Biological characteristics. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500007 Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections BJID Stehling,Eliana Guedes; Silveira,Wanderley Dias da; Leite,Domingos da Silva. A total of 120 strains of Pseudomonas aeruginosa, isolated from cystic fibrosis (CF) patients (n = 80) and from patients having extra-pulmonary infections (n = 40) were studied regarding the presence of some virulence factors (hemolysin, gelatinase and elastase production) and presence of the algD and algU genes as detected by polymerase chain reaction-PCR. There was not a significant difference for the production of gelatinase and hemolysin between non-mucoid strains from CF patients and other isolates from extra-pulmonary infections and mucoid strains. The production of elastase was found to be significant among these strains. The algD gene was detected by PCR in all studied strains but the algU gene was detected only in 25% of the mucoid strains.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biological characterization; Pathogenesis; Cystic fibrosis; Extra-pulmonary infections. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100018 Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa BJID Perez,Leandro Reus Rodrigues; Barth,Afonso Luís. Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of these isolates for eight antimicrobial agents. The ability for biofilm production when TSB medium was used was higher than when used CF sputum media (p = 0.0198). However, P. aeruginosa isolates from CF have demonstrated similar performance for biofilm production, independently of the medium used. Besides, among the biofilm-producing isolates, those recovered from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Pseudomonas aeruginosa; Cystic fibrosis; Antimicrobial resistance. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400001 Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de. ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS). Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129 Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection BJMBR Zanetti,C.C.S.; Mingrone,R.C.C.; Kisielius,J.J.; Ueda-Ito,M.; Pignatari,A.C.C.. Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteriophages; Pseudomonas aeruginosa. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800689 Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype BJMBR Freitas,A.L.; Barth,A.L.. Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the "gold standard" for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Typing; Genotyping; Phenotypic markers. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000100011 Nutritional requirement among Pseudomonas aeruginosa isolates recovered from respiratory clinical specimens at a tertiary hospital from South of Brazil BJM Perez,Leandro Reus Rodrigues; Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luís. We screened 349 isolates of P. aeruginosa from cystic fibrosis (CF+) and non-cystic fibrosis (CF-) patients for the auxotrophy. Fourteen (4.0%) were auxotrophic and among them only one was recovered from CF-patient showing that this characteristic is strongly associated with cystic fibrosis. In total, a requirement for 5 different compounds (or combination) was verified and, of these, methionine was the most common single amino acid required. Only one auxotrophic isolate was no able to produce biofilm in vitro. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Auxotrophy; Cystic fibrosis; Biofilm. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300026 Nosocomial outbreaks due to Pseudomonas aeruginosa and Acinetobacter baumannii in a Neonatal Intensive Care Unit (NICU) of the Uberlândia Federal University Hospital BJM Brito,Denise Von Dolinger de; Oliveira,Elias Jose; Darini,Ana Lúcia da Costa; Abdallah,Vânia Olivetti Sttephen; Gontijo-Filho,Paulo Pinto. The study documents the occurrence of two subsequent outbreaks in the NICU of HC-UFU, caused by epidemic strains of multiresistant Pseudomonas aeruginosa and Acinetobacter baumannii, that occurred between March/01 and September and between October/01 and March/02, respectively. The P. aeruginosa outbreak included seven neonates with conjunctivitis and three with bacteremia. A case-control study was conducted for the A. baumannii outbreak, with 11 and 22 neonates, respectively. The isolates of A. baumannii were resistant to gentamacin and ciprofloxacin. P. aeruginosa isolates were resistant to ampicillin/sulbactam gentamicin and ciprofloxacin. The hands of healthcare workers and environmental cultures were negative. The outbreak of P. aeruginosa resulted in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Outbreak; Neonate; Acinetobacter baumannii; Pseudomonas aeruginosa. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500009 Carbapenem-resistant Pseudomonas aeruginosa: association with virulence genes and biofilm formation BJM Gonçalves,Iara Rossi; Dantas,Raquel Cristina Cavalcanti; Ferreira,Melina Lorraine; Batistão,Deivid William da Fonseca; Gontijo-Filho,Paulo Pinto; Ribas,Rosineide Marques. Abstract Pseudomonas aeruginosa is an opportunistic pathogen that causes frequently nosocomial infections, currently becoming more difficult to treat due to the various resistance mechanisms and different virulence factors. The purpose of this study was to determine the risk factors independently associated with the development of bacteremia by carbapenem-resistant P. aeruginosa, the frequency of virulence genes in metallo-β-lactamases producers and to evaluate their ability to produce biofilm. We conducted a case–control study in the Uberlândia Federal University – Hospital Clinic, Brazil. Polymerase Chain Reaction was performed for metallo-β-lactamases and virulence genes. Adhesion and biofilm assays were done by quantitative tests. Among the 157 strains... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Resistance; Metallo-β-lactamases; Virulence; Biofilm. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200211 Effects of growth temperature on polystyrene adhesion of Pseudomonas aeruginosa ATCC 27853 BJM Cappello,Simone; Guglielmino,Salvatore P.P.. The importance of avoiding bacterial adhesion to plastic medical devices is recognized. To better understand the interaction between bacteria and surface, we studied the influence of different growth temperatures (15, 30 and 47ºC) on the polystyrene adhesion capacity of Pseudomonas aeruginosa ATCC 27853. Tipo: Info:eu-repo/semantics/other Palavras-chave: Pseudomonas aeruginosa; Temperature; Hydrophobicity; Adhesion. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300001 Registros recuperados: 66 Primeira ... 1 2 3 4 ... Última

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