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Registros recuperados: 39
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Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy Naturalis
Carlucci, A.; Raimondo, M.L.; Santos, J.; Phillips, A.J.L..
Plectosphaerella cucumerina, most frequently encountered in its Plectosporium state, is well known as a pathogen of several plant species causing fruit, root and collar rot, and collapse. It is considered to pose a serious threat to melon (Cucumis melo) production in Italy. In the present study, an intensive sampling of diseased cucurbits as well as tomato and bell pepper was done and the fungal pathogens present on them were isolated. Phylogenetic relationships of the isolates were determined through a study of ribosomal RNA gene sequences (ITS cluster and D1/D2 domain of the 28S rRNA gene). Combining morphological, culture and molecular data, six species were distinguished. One of these (Pa. cucumerina) is already known. Four new species are described as...
Tipo: Article / Letter to the editor Palavras-chave: D1/D2; ITS; LSU; Phylogeny; Plectosporium; RDNA; Systematics; Taxonomy.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/531914
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The phylogenetic position of Artematopodidae (Coleoptera: Elateroidea), with description of the first two Eurypogon species from China Naturalis
Kundrata, R.; Bocakova, M.; Bocak, L..
The Artematopodidae is a species-poor beetle family with contentious relationships to byrrhoid and elateroid families. Recent molecular phylogenetic analyses brought ambiguous results based on a single sequenced species. We investigated the taxonomic placement of Artematopodidae within Elateriformia using ribosomal (18S, 28S) and mitochondrial (rrnL, cox1) molecular markers and three artematopodid species. Our analyses placed Artematopodidae close to Omethidae+Telegeusidae in a basal position of broadly defined Elateroidea. Additionally, we described the first artematopodid species from China – Eurypogon jaechi sp. nov. and E. heishuiensis sp. nov. These species are reported from mountains of Yunnan and can be easily distinguished from their Palaearctic...
Tipo: Article / Letter to the editor Palavras-chave: MtDNA; RDNA; Diversity; Elateriformia; New species; Palaearctic Region; Phylogeny; Taxonomy; 42.75; 42.64.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/462591
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A new species of bird’s nest fungi: characterisation of Cyathus subglobisporus sp. nov. based on morphological and molecular data Naturalis
Zhao, R.-L.; Desjardin, D.E.; Soytong, K.; Hyde, K.D..
Recent collections of bird’s nest fungi (i.e. Crucibulum, Cyathus, Mycocalia, Nidula, and Nidularia species) in northern Thailand resulted in the discovery of a new species of Cyathus, herein described as C. subglobisporus. This species is distinct by a combination of ivory-coloured fruiting bodies covered with shaggy hairs, plications on the inner surface of the peridium and subglobose basidiospores. Phylogenetic analyses based on ITS and LSU ribosomal DNA sequences using neighbour-joining, maximum likelihood and weighted maximum parsimony support Cyathus subglobisporus as a distinct species and sister to a clade containing C. annulatus, C. renweii and C. stercoreus in the Striatum group.
Tipo: Article / Letter to the editor Palavras-chave: Bird’s nest fungi; Gasteromycetes; New species; Phylogeny; RDNA.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/532073
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Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home Naturalis
Benzoni, F.; Arrigoni, R.; Stefani, F.; Reijnen, B.T.; Montano, S.; Hoeksema, B..
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...
Tipo: Article / Letter to the editor Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA; 42.72.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/421558
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Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae) Naturalis
Arrigoni, R.; Richards, Z.T.; Chen, C.A.; Baird, A.H.; Benzoni, F..
Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...
Tipo: Article / Letter to the editor Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision; 42.72.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/499033
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Phylogenetic and morphological assessment of two new species of Amniculicola and their allies (Pleosporales) Naturalis
Zhang, Y.; Fournier, J.; Crous, P.W.; Pointing, S.B.; Hyde, K.D..
Two new species of Amniculicola, A. immersa sp. nov. and A. parva sp. nov. from submerged wood in a freshwater environment in Denmark and France are respectively described and illustrated. In addition, partial 28S rDNA sequence data is analysed to investigate their phylogenetic relationships with other pleosporalean taxa. All presently known Amniculicola species, A. immersa, A. lignicola and A. parva, form a robust clade together with the anamorphic species Anguillospora longissima, Spirosphaera cupreorufescens and Repetophragma ontariense. These six species, which are all from freshwater and mostly from Europe, constitute a well-supported group containing Pleospora rubicunda and Massariosphaeria typhicola. This putative monophyletic assemblage may...
Tipo: Article / Letter to the editor Palavras-chave: Anamorphs; Freshwater fungi; Phylogeny; Pseudoparaphyses; RDNA.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/531919
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Molecular Tool for Identification of Closely Related Species of Trichogramma (Hymenoptera: Trichogrammatidae): T. rojasi Nagaraja & Nagarkatti and T. lasallei Pinto Neotropical Entomology
CIOCIOLA JR.,AMÉRICO I.; QUERINO,RANYSE B.; ZUCCHI,ROBERTO A.; STOUTHAMER,RICHARD.
The sequence of the ITS2 (internally transcribed spacer 2) region of the rDNA was used to identify two closely related Trichogramma species: T. rojasi and T. lasallei, the second species recently reported in Brazil. The DNA of the samples was extracted using Chelex 100. The PCR product was linked to a pGEM-T® vector and both were then placed into Petri dishes to grow white colonies, which demonstrate the correct ligation between the vector and the DNA. From these colonies, 10µl were purified and sequenced using a special kit, and the sequences aligned using the ESEE 3.0 software. Specific primers were efficient to amplify the ITS2 rDNA region of the Brazilian Trichogramma. The sequence of each species differed in length and in nucleotide position, showing...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Insecta; ITS2 region; Biological control; RDNA; Parasitoids.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000400010
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Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae) Neotropical Ichthyology
Marques,Debora Karla; Venere,Paulo Cesar; Galetti Junior,Pedro Manoel.
The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pirarucu; Karyotype; RDNA; Heterochromatin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200007
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Cytogenetic analysis of Astyanax laticeps (Cope, 1894) (Ostariophysi: Characidae) from the laguna dos Patos system Neotropical Ichthyology
Rosa,Renata da; Rubert,Marceléia; Malabarba,Luiz R.; Martins-Santos,Isabel Cristina; Giuliano-Caetano,Lucia.
The genus Astyanax is prominent among Characiformes, due to the large number of species found and its wide geographic distribution. In this work, Astyanax laticeps specimens from the laguna dos Patos system were cytogenetically analyzed. A diploid number of 2n = 50 chromosomes distributed into 6m+16sm+16st+12a (FN = 88) was found, without differences between males and females. A few small heterochromatin blocks were observed, besides three more conspicuous C-bands, corresponding to NORs, as confirmed by silver nitrate and CMA3 staining, FISH, and DAPI negative staining. These regions were located in a medium-sized subtelocentric and in a large subtelocentric chromosomal pair, probably because of a deletion of this region in one homologous chromosome, or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax scabripinnis species complex; RDNA; Homologous transposition.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400007
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Karyotype analysis of three species of Corydoras (Siluriformes: Callichthyidae) from southern Brazil: rearranged karyotypes and cytotaxonomy Neotropical Ichthyology
Barbosa,Patrícia; Pucci,Marcela B.; Nogaroto,Viviane; Almeida,Mara C.; Artoni,Roberto F.; Vicari,Marcelo R..
ABSTRACT The genus Corydoras comprises a diversity of species with different diploid numbers. We compared cytogenetic data among Corydoras species from different rivers of the Ponta Grossa Arch region in southern Brazil. Corydoras ehrhardti and C. aff. paleatus have a similar karyotype formula and the same diploid number (2n = 44). Corydoras lacrimostigmata has a higher diploid number, with 2n = 58 chromosomes. Fluorescence in situ hybridization using 5S and 18S ribosomal DNA probes suggests that these ribosomal DNA sequences are involved in chromosomal rearrangements in these Corydoras species. 5S rDNA is a chromosomal marker that is considered to be unique to the species analyzed in this study. Signals of interstitial telomeric sites are seen in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytosystematics; Heterokaryotypes; Karyotype description; RDNA; Vicariance.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100222
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Cytogenetic analysis of Baryancistrus xanthellus (Siluriformes: Loricariidae: Ancistrini), an ornamental fish endemic to the Xingu River, Brazil Neotropical Ichthyology
Medeiros,Larissa A.; Ginani,Eduardo G.; Sousa,Leandro M.; Py-Daniel,Lúcia H. Rapp; Feldberg,Eliana.
ABSTRACT Baryancistrus xanthellus is a species from the Ancistrini tribe known commonly as "amarelinho " or "golden nugget pleco". It is one of the most popular and valued ornamental fishes due to its color pattern. Also, it is an endemic species from the Xingu River occurring from Volta Grande do Xingu, region where the Belo Monte Hydropower Dam is being built, to São Félix do Xingu. The current study aimed to cytogenetically characterize B. xanthellus . Results point to the maintenance of 2n=52, which is considered the most common condition for the tribe, and a single nucleolus organizer region (NOR). Mapping of the 18S rDNA confirmed the NOR sites, and the 5S rDNA was mapped in the interstitial position of a single chromosome pair. The 18S and 5S rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FISH; Habitat endangered; RDNA; Volta Grande do Xingu.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200212
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Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) Neotropical Ichthyology
Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto.
Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential R. Bras. Zootec.
Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303
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Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub Scientia Agricola
Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro.
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; RDNA; SSR; Infection courts.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200
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Two new species of the industrially relevant genus Absidia (Mucorales) from soil of the Brazilian Atlantic Forest Acta Botanica
Cordeiro,Thalline Rafhaella Leite; Nguyen,Thuong Thuong Thi; Lima,Diogo Xavier; Silva,Suzana Brito Gomes da; Lima,Catarina Ferreira de; Leitão,Joana D’Arc Alves; Gurgel,Luciana Melo Sartori; Lee,Hyang Burm; Santiago,André Luiz Cabral Monteiro de Azevedo.
ABSTRACT During a survey of Mucorales in soil from an upland forest area in Pernambuco, Brazil, two specimens were isolated and characterized based on their morphological, physiological, and molecular data (ITS and LSU rDNA). Phylogenetic analyses of the isolates revealed that the strains URM 8209 and URM 8210 are closely related to species of Absidia. URM 8209 forms conical, subglobose, and strawberry-shaped columellae and the sporangiospores are cylindrical and ellipsoid. URM 8210 produces hemispheric, subglobose, and strawberry-shaped columellae and the sporangiospores are globose, subglobose, ellipsoid, and short cylindrical. Based on evidence obtained through analysis of datasets (LSU and ITS rDNA regions), A. saloaensis sp. nov. (URM 8209) and A....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cunninghamellaceae; Mucoromyceta; RDNA; Soil; Taxonomy.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000300549
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Karyotype characterization and evolution of chromosome number in Cactaceae with special emphasis on subfamily Cactoideae Acta Botanica
Castro,Juliana P.; Moraes,Ana Paula; Chase,Mark W.; Santos,Angeline M. S.; Batista,Fabiane R. C.; Felix,Leonardo P..
ABSTRACT Cactaceae species are karyotypically well-known with x = 11 and chromosome number variation due mainly to polyploidization. However, both assumptions are based on descriptive observations without taking an evolutionary framework of Cactaceae into account. Aiming to confirm these hypotheses in an evolutionary context, we obtained chromosome numbers for 20 species of Cactoideae, performed an extensive review of chromosome number for the family, and analyzed these data using a phylogenetic approach. The karyotypes presented here were characterized by CMA/DAPI banding, and for six species 5S and 45S rDNA sites were located. Our data, along with a survey of the literature, reinforce the long-standing hypothesis of a x = 11 as the base chromosome number...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Base chromosome number; CMA/DAPI; Dysploidy; Heterochromatin; Polyploidy; RDNA.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100135
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Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii - DOI: 10.4025/actascibiolsci.v25i2.2037 Biological Sciences
Gasques, Luciano Seraphim; Universidade Paranaense; Fernandez, Maria Aparecida; UEM; Correa, Luiz Carlos; UEM.
A definição e a caracterização de regiões de origens de replicação nos eucariotos superiores são ainda controversas. A iniciação da replicação é sítio-específica em alguns sistemas e, em outros, parece estar contida em regiões extensas. Regiões rDNA são modelos atrativos para o estudo de origens de replicação pela sua organização in tandem, reduzindo a área de estudo para o espaço restrito que codifica uma unidade de transcrição. Neste trabalho nós isolamos e caracterizamos parcialmente um clone que contém uma sequência ribossomal do fungo aquático Blastocladiella emersonii, Be97M20. Southern blots mostraram diversos sítios para enzimas de restrição Eco RI, HindIII e SalI. Northern blot de RNA total hibridado contra uma sonda feita com Be97M20 confirmou a...
Palavras-chave: 2.01.00.00-0 Biologia Geral Blastocladiella emersonii; RDNA; Genes ribossomais 2.01.00.00-0 Biologia Geral.
Ano: 2003 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2037
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Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio Biol. Res.
VERA,MARÍA INÉS; MOLINA,ALFREDO; PINTO,RODRIGO; REYES,MAURICIO; ÁLVAREZ,MARCO; KRAUSKOPF,ERWIN; QUEZADA,CLAUDIA; TORRES,JORGE; KRAUSKOPF,MANUEL.
The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression...
Tipo: Journal article Palavras-chave: Ribosomal gene; RRNA; RDNA; Fish; Carp.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014
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Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences BABT
Lopez,Ana Maria Queijeiro; Lucas,John Alexander.
Thirty six isolates of fungi obtained from anthracnose lesions of cashew and associated host plants in Brazil, were compared by their cultural, morphological and partial sequences of the 28S ribosomal DNA characters. They showed a high degree of cultural variability. The average mycelial growth rate on all tested media ranged from 10.2-13.3 mm/day between the isolates. Most of them produced perithecia (sterile and fertile) and some produced setae (sterile and fertile). All the isolates produced acervuli with predominantly cylindrical conidia (12.4-17.7 µmX 4.8-6.0 µm in width) with round ends, which became septate on germination, and produced unlobed or slightlylobed appressoria. Comparison of the D2 domain of the large subunit (LSU) rDNA sequences with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracnose; Inflorescence blight; Colletotrichum; RDNA; Cashew; Anacardium occidentale.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400001
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