|
|
|
| Registros recuperados: 24 | |
|
|
| Rocha,Antonio José; Maranhão,Paulo Abraão; Silva,Rafaela Oliveira; Pohl,Simone; Fonteles,Cristiane S.R. |
| Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues. Three softwares were utilized to verify which would be the most stable reference genes in qPCR assay: GeNorm, NormFinder and BestKeeper. The most stable reference genes in developing J. curcas seeds suggested by GeNorm were GAPDH, UCP, actin. However, the best combinations of stable genes in each tissue were identified separately under stress conditions: EF1-α, PP2A2 and GAPDH in total stress, however, in SA stress, four genes were required for normalization: PP2A2, EF1-α, GAPDH and PUB. In PEG stress, four genes also were required: PP2A2, EF1-α, GAPDH and PUB, while in NaCl... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; RT-qPCR; Reference genes; Jatropha curcas L. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100404 |
| |
|
| |
|
| |
|
|
| Abreu,José Renato de; Paiva,Luciano Vilela; Rodríguez,Miguel Angel Dita; Silva,Anderson Tadeu; Henriques,Ariadne Ribeiro; Chalfun-Junior,Antonio. |
| Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Differential gene expression; Subtractive hybridization; RT-qPCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000100032 |
| |
| Registros recuperados: 24 | |
|
|
|
