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| Registros recuperados: 58 | |
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| Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C.. |
| Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also... |
| Tipo: Text |
Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf |
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| Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique. |
| Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V.... |
| Tipo: Text |
Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf |
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| De Decker, Sophie; Saulnier, Denis. |
| The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered... |
| Tipo: Text |
Palavras-chave: Oyster-Vibrio interactions; Pathogenesis; Immune response; Non-invasive experimental challenge; Real-time PCR. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00025/13604/10681.pdf |
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| Demirbilek,SK; Ardicli,Ö; Carli,KT. |
| ABSTRACT This study aimed to compare method-based and newly developed sample-based methods for Mycoplasma gallisepticum (MG) detection in different samples of breeder flocks suffering from respiratory disease problems by using culture, real-time PCR (rPCR) and ELISA from chicks and embryonated eggs. Overall, 450 samples of 19-day-old chicken embryo’s trachea, 450 samples of 8-day-old chicken tracheal swabs and 900 blood samples of 20-, 27-, 34-, 40- and 46-week-old breeder chickens from 5 flocks were sampled for 26 weeks, and were all tested for MG by culture, MG-rPCR and MG-ELISA. Culturing assays and rPCR were applied to 450 mixture samples from 19-day-old chicken embryo’s trachea and 450 tracheal swab samples (each pooled into groups of 3) from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture; Egg yolk; Mycoplasma gallisepticum; Real-time PCR; Tracheal swab. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200320 |
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| Huo,Sheng-dong; Zhang,Tao-jie; Abudureyimu,Ayimuguli; Liu,Jun-lin; Zhang,Guo-hua; Ma,Zhong-ren. |
| ABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bos grunniens; Gonadotropin-releasing hormone receptor; Immunofluorescence; Real-time PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401 |
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| Huo,Shengdong; Yuan,Qijian; Zhang,Taojie; Liu,Junlin; Abudureyimu,Ayimuguli; Yang,Jutian. |
| ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunofluorescence; Real-time PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
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| Villar-Luna,Edgar; Rojas-Martínez,Reyna I.; Reyes-Trejo,Benito; Rocha-Sosa,Mario; Zavaleta-Mejía,Emma. |
| Plants of chilli Capsicum annuum L. CM334 show a high level of resistance to Phytophthora capsici, but are susceptible to Nacobbus aberrans; such resistance has been associated with capsidiol accumulation, a sesquiterpene phytoalexin. In the present study the expression of hydroxymethylglutaryl-CoA reductase 2 gene (HMG2, associated with sesquiterpene phytoalexins biosynthesis) in CM334 chilli roots inoculated with N. aberrans and in combination with P. capsici, was determined by qRT-PCR. The levels of HMG2 transcripts were significantly reduced (p≤0.05) from -1.57 to -1.28-fold in the interaction CM334/N. aberrans; whereas in roots inoculated only with the oomycete, the accumulation of transcripts occurred earlier and at higher levels (1.52 to 3.54-fold).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum annuum; Capsidiol; Real-time PCR; Root-knot nematodes. |
| Ano: 2015 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000100005 |
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| Wa Lee,Young H; Am Na,Hee S; Jeong,So Y Eon; Jeong,Sung H Ee; Park,Hae R Youn; Chung,Jin. |
| MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA microarray was carried out and the expression of miRNAs was confirmed by real-time PCR. According to miRNA microarray analyses, six miRNA genes, let-7a,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microarray; Real-time PCR; Gingiva. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200002 |
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| Carnielli-Queiroz,Lorena; Fernandes,Patricia Machado Bueno; Fernandes,Antônio Alberto Ribeiro; Ventura,José Aires. |
| Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity test was conducted with slips of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnostic; Diseases; Quarantine; Real-time PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100219 |
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| Hosseinzadeh,Ali; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Seifi,Morteza; Gavgani,Reyhaneh Ravanbakhsh; Nadergoli,Omid Kheyri; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim. |
| Many cancer researchers use gene expression analysis for differentiation between tumor and normal cells for diagnostic, prognostic and therapeutic purposes. Most of studies compare either tumor cell lines by normal cell lines or tumor tissue of affected individuals by normal healthy control tissue. But expression of each special gene is unique in different individuals and also in different tissue of same individual. For this reason, here we compare the gene expression levels of SMAD7 and KLF10 in tumor cells and its adjacent normal tissue of breast cancer patients and compared them. For this purpose, a total of 40 tumor and matched tumor-free margin samples were obtained during surgery. The SMAD7 and KLF10 mRNA expression levels in tumor and marginal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: KLF10; Personalized gene Expression; Real-time PCR; SMAD7; TGFβ. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100317 |
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| Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate. |
| ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454 |
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| Registros recuperados: 58 | |
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