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| Registros recuperados: 25 | |
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| MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
| Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
| Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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| Pérez,Rodrigo; Navarro,Florentino; Sánchez,María Ángeles; Ortíz,Jesús María; Morales,Remedios. |
| The rapid identification of the hybrids between sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) is not easy. In order to resolve this problem, 18 Spanish sweet, sour and duke cherry cultivars were surveyed and characterized using 43 agromorphological descriptors evaluated in flowers, leaves, dormant 1-yr-old shoots, fruits, and trees during 2005 and 2006. Based on quantitative parameters, ANOVA and stepwise discriminant analysis (SDA) were carried out. For qualitative descriptors, statistical comparisons were done by means of the chi-square (χ2) test. As result of the study, two quantitative (titratable acidity and number of lenticels) and six qualitative descriptors (shape of the central and lateral lobes in the internal bracts... |
| Tipo: Journal article |
Palavras-chave: Rosaceae; Characterization; Quantitative parameters; Qualitative parameters; SDA analysis. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000100004 |
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| Reis,Michele Valquíria dos; Rouhana,Laura Vaughn; Paiva,Patrícia Duarte de Oliveira; Silva,Diogo Pedrosa Correia da; Paiva,Renato; Korban,Schuyler. |
| ABSTRACT Rugosa rugosa has high tolerance to various stresses; however, the molecular mechanisms of this behavior under adverse conditions are unclear. The objective of this study is to investigate expression patterns of stress-related genes in response to salinity stress. Changes in transcript levels of R. rugose, grown under different salt stress conditions (0, 25, 50, and 100 mM NaCl) over a long exposure period (30 days), have been investigated. In addition, the effects of salt shock stress on seedlings exposed to a high level (200 mM) of NaCl for a relatively short duration (3 h) have also been investigated. Expression levels of selected differentially expressed genes have been determined using relative reverse transcription polymerase chain reaction... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rosaceae; Salinity; Salt tolerance; Ion homeostasis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100241 |
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| Registros recuperados: 25 | |
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