SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Reynders, M.; Goetghebeur, P..

Pycreus sect. Tuberculati was created by Chermezon to contain a single species with derived nutlets: P. divulsus, a Malagasy endemic. Kükenthal transferred this species to his new section Muricati. However, a detailed study of the nutlet epidermis shows P. divulsus is not closely related to the other species Kükenthal placed in the latter section. In addition P. divulsus subsp. africanus is upgraded to the species level based on its larger, smooth nutlets and African continental range.

Floral development in three species of Impatiens (Balsaminaceae)

Palavras-chave: Africa; Cyperaceae; Pycreus sect. Tuberculati; Pycreus africanus; Pycreus divulsus; Scanning electron microscopy; Taxonomy.

Ano: 2010

URL: http://www.repository.naturalis.nl/record/525530

Caris, P.L.; Geuten, K.P.; Janssens, S.B.; Smets, E..

The floral morphological and developmental patterns in three species of Impatiens (Balsaminaceae), namely I. columbaria, I. hawkeri, and I. niamniamensis, were studied to contribute to a better understanding of floral evolution in the genus. Strangely enough, the highly diverse floral morphology and ontogeny of this horticulturally important genus have never been studied thoroughly (e.g., using scanning electron microscopic techniques). We discuss the position and the developmental sequence of the different perianth members. We hypothesized that in the course of evolution, the anterolateral sepals become reduced and that a morphocline can be recognized going from species with five sepals, over species with rudimentary sepals that fuse postgenitally with...

Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization

Palavras-chave: Balsaminaceae; Ericales; Floral development; Floral evolution; Floral morphology; Impatiens; Scanning electron microscopy; 42.40.

Ano: 2006

URL: http://www.repository.naturalis.nl/record/422332

Hubka, V.; Barrs, V.; Dudová, Z.; Sklenář, F.; Kubátová, A.; Matsuzawa, T.; Yaguchi, T.; Horie, Y.; Nováková, A.; Frisvad, J.C.; Talbot, J.J.; Kolařík, M..

Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC). Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in the AVSC are unresolved since most species have uniform morphology and produce interspecific hybrids in vitro. Clinical and environmental strains from six continents (n = 110) were characterized by DNA sequencing of four to six loci. Biological compatibilities were tested within and between major phylogenetic clades, and ascospore morphology was characterised. Species delimitation methods based on the multispecies...

Floral Ontogeny in Scirpus, Eriophorum and Dulichium (Cyperaceae), with Special Reference to the Perianth

Palavras-chave: Aspergillus felis; Aspergillus fumigatus; Invasive aspergillosis; Mating-type genes; Multispecies coalescence model; Neosartorya udagawae; Scanning electron microscopy; Soil fungi.

Ano: 2018

URL: http://www.repository.naturalis.nl/record/652411

Vrijdaghs, A.; Caris, P.; Goetghebeur, P.; Smets, E..

Background and Aims Based on molecular phylogenetic analysis, it has been suggested recently that the Cyperaceae comprises only two subfamilies: the Mapanioideae and the Cyperoideae. In most flowers of the Cyperoideae, the whorl of inner stamens is reduced, resulting in tetracyclic flowers. In the more primitive (scirpoid) genera within the Cyperoideae, the perianth consists of two polysymmetric whorls, whereas the perianth parts in the more derived genera have been subject to modifications and/or reduction. Comparative studies of the many silky hairs of Eriophorum and of the eight bristles of Dulichium have given rise to much discussion about their homology. Methods The spikelet and floral ontogeny in freshly collected inflorescences was investigated...

Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.)

Palavras-chave: Dulichium arundinaceum (L.) Britton; Eriophorum latifolium Hoppe; Floral ontogeny; Perianth; Scirpoid flower; Scirpus sylvaticus L; Scanning electron microscopy.

Ano: 2005

URL: http://www.repository.naturalis.nl/record/407290

Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E..

The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...

Floral Ontogeny in Ficinia and Isolepis (Cyperaceae), with Focus on the Nature and Origin of the Gynophore

Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.

Ano: 2005

URL: http://www.repository.naturalis.nl/record/407307

Vrijdaghs, H.; Goetghebeur, P.; Muasya, A.M.; Caris, P.; Smets, E..

! Background and Aims The generic delimitations of Ficinia and Isolepis, sister genera in the Cypereae, are blurred. Typical Ficinia flowers have a lobed gynophore, which envelops the base of the nutlet, whereas in Isolepis the character is considered to be absent. Some former species of Isolepis, lacking the gynophore, were recently included in Ficinia. The floral ontogeny of representative taxa in Ficinia and Isolepis were investigated with the aim of evaluating the origin and nature of the gynophore in the Cypereae. ! Methods The spikelet and floral ontogeny in inflorescences collected in the field was investigated using scanning electron microscopy (SEM) and light microscopy (LM). ! Key Results SEM images of Isolepis setacea and I. antarctica, Ficinia...

Synaphridae of Madagascar (Araneae: Araneoidea): A New Family Record for the Afrotropical Region

Palavras-chave: Ficinia; Floral ontogeny; Gynophore; Isolepis; Scanning electron microscopy.

Ano: 2005

URL: http://www.repository.naturalis.nl/record/407286

Miller, J.A..

The spider family Synaphridae is reported for the first time from Madagascar. Three new species are described. Africepheia gen. nov. is established for A. madagascariensis sp. nov.; two species of Synaphris Simon, 1894 are described: S. schlingeri sp. nov. and S. toliara sp. nov. Diagnostic characteristics for the family and putative synapomorphies are discussed, elucidated by scanning electron microscope images representing both Malagasy genera. This research highlights the connection between large scale collecting efforts in biodiversity hotspots like Madagascar and advances in systematics.

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Palavras-chave: Taxonomy; Synapomorphy; Scanning electron microscopy; Africepheia; Cepheia; Synaphris; 42.74.

Ano: 2007

URL: http://www.repository.naturalis.nl/record/306631

Neotropical Entomology

Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A..

The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...

Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion

Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009

PAB

Costa,Maria Angélica Pereira de Carvalho; Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli.

The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field.

Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid

Palavras-chave: Stoma; Somatic hybridization; Scanning electron microscopy; Polyploidy.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000300015

Rev. Bras. Ciênc. Avic.

Fernandes,BCS; Martins,MRFB; Mendes,AA; Milbradt,EL; Sanfelice,C; Martins,BB; Aguiar,EF; Bresne,C.

This study aimed at evaluating the intestinal integrity, using light and scanning electron microscopy (SEM), and the performance of broiler chickens fed additives alternative to antimicrobials. A total of 1080 male chicks were distributed according to a completely randomized experimental design, with six treatments with six replicates of 30 birds each. The following treatments were evaluated: basal diet (control), basal diet supplemented with an antimicrobial, basal diet supplemented with a probiotic, basal diet supplemented with a prebiotic, basal diet with a symbiotic, and basal diet supplemented with organic acids. Weight gain, feed intake, feed conversion ratio and livability were recorded when broiler chickens were 10, 21, 35, and 42 days old. On day...

Chemical-Mineralogical Characterization of Magnetic Materials from Magnetic Soils of the Southern Espinhaço Mountain Chain and of the Upper Jequitinhonha Valley, State of Minas Gerais, Brazil

Palavras-chave: Scanning electron microscopy; Morphometrics; Intestinal mucosa; Intestinal wall.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400012

Rev. Bras. Ciênc. Solo

Silva,Alexandre Christófaro; Souza,Solange de; Fabris,José Domingos; Barbosa,Maurício Soares; Barral,Uidemar Morais; Costa,Roberto Vial.

ABSTRACT In the Southern Espinhaço Mountain Chain and in the Upper Jequitinhonha Valley, magnetic soils, in different pedogenetic stages, are found to be forming over intrusions of basic lithology. The essential chemical and mineralogical properties of samples from magnetic soil profiles from those two physiographic environments in the state of Minas Gerais, Brazil, are reported. Three of the pedons (Rhodic Kandiustox – RKox, Rhodic Haplustox – RHox, and Typic Argiustoll - TAoll) were identified as being indeed developed over basic rocks; the fourth pedon (Typic Haplustox - THox) is currently forming on an acidic rock. Particle size and routine chemical analyses were performed on samples from all horizons of the four selected soil profiles. For a deeper...

Ultrastructure of the antennal sensilla of Alabama argillacea (Hübner, 1823) (Lepidoptera: Erebidae)

Palavras-chave: Iron oxides; X ray diffractometry; Mössbauer spectroscopy; Scanning electron microscopy; Energy dispersive spectroscopy.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100305

Rev. Bras. entomol.

Santos,Andrezo Adenilton; Wanderley-Teixeira,Valéria; Cruz,Glaucilane dos Santos; Navarro,Daniela Maria do Amaral Ferraz; Alves,Luiz Carlos; Brayner,Fábio André; Teixeira,Álvaro Aguiar Coelho.

Abstract Insects have several types of sensilla, the characterization of which has been fundamental to understanding the mechanisms of sensory perception in different species. This study aimed to describe the ultrastructure of the sensilla present on the antennae of Alabama argillacea (Hübner, 1823) (Lepidoptera: Erebidae), an important pest of cotton (Gossypium hirsutum L.) crops, as well as their possible variation between sexes. To do this, the antennae of males and females of A. argillacea were analyzed using scanning electron microscopy. Sensilla morphometry was assessed using photomicrographs, from which the lengths and basal and apical diameters of sensilla were measured using the ImageJ program. Seven types of sensilla were identified on the...

Survey of the leg exocrine glands in termites(Isoptera)

Palavras-chave: Antenna; Cotton leafworm; Morphometry; Scanning electron microscopy.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200141

Rev. Bras. entomol.

Soares,Helena Xavier; Costa-Leonardo,Ana Maria.

A survey of the leg exocrine glands in the termite workers of 16 species of the families Kalotermitidae and Termitidae was carried out through scanning electron microscope. Glandular openings were not found in the legs of Anoplotermes sp., Ruptitermes sp. (Apicotermitinae, Termitidae) or Glyptotermes planus (Kalotermitidae), but they are present, spread over the ventral surface of the first, second and third tarsomeres of other Termitidae such as Armitermes euamignathus, Cornitermes cumulans, Nasutitermes coxipoensis, Rhynchotermes nasutissimus, Syntermes nanus, Embiratermes festivellus (Nasutitermitinae), Amitermes beaumonti, Hoplotermes amplus, Microcerotermes sp., Neocapritermes opacus, Orthognathotermes sp., Spinitermes brevicornutus and Termes sp....

Morphology, distribution and abundance of antennal sensilla of the oyster mushroom fly, Coboldia fuscipes (Meigen) (Diptera: Scatopsidae)

Palavras-chave: Exocrine gland; Leg; Morphology; Scanning electron microscopy; Termite.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262002000100001

Rev. Bras. entomol.

Zhang,Zhilin; Li,Xinlian; Chen,Lizhen; Wang,Lihua; Lei,Chaoliang.

ABSTRACT We investigated the distribution, morphology and abundance of antennae sensilla of Coboldia fuscipes (Meigen) using scanning electron microscopy. Antennae of C. fuscipes consisted of scape, pedicel, and flagellum with eight flagellomeres. Antennal scape and pedicel had only one type of sensillum, i.e., sensilla chaetica. Significant differences were found between the number and distribution of these sensilla. Four types of morphologically distinct sensilla on the flagellum were identified, including sensilla chaetica, sensilla trichoidea, sensilla coeloconica, and sensilla basiconica (three subtypes). Significant differences were found in the abundance and distribution of sensilla among the antennal flagella and diverse flagellomeres in both...

Use of scanning electron microscopy for the evaluation of intestinal epithelium integrity

Palavras-chave: Antennae; Flagellum; Scanning electron microscopy.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000100008

R. Bras. Zootec.

Gomide Junior,Marcio Heber; Sterzo,Elton Vinícius; Macari,Marcos; Boleli,Isabel Cristina.

This work reports the use of scanning electron microscopy to evaluate intestinal epithelium loss in broilers. Intestinal mucosa of male and female chicks submitted to prolonged period post-hatching of water and feed deprivation was evaluated. Two segments of the duodenum, jejunum and ileum were taken per bird, and routinely processed by scanning electron microscopy. Six distinct degrees of epithelium loss were determined: degree 0, normal villi without apparent extrusion; degree 1, villi presenting small areas of epithelium loss; degree 2, villi without epithelium at the apex; degree 3, villi without epithelium at the apical region; degree 4, villi without epithelium in the upper half; degree 5, lack of epithelium in the whole villus; degree 6, loss of...

Origin of microaggregates in soils with ferralic horizons

Palavras-chave: Broilers; Fasting; Intestinal epithelium loss; Scanning electron microscopy.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600017

Cooper,Miguel; Vidal-Torrado,Pablo; Chaplot,Vincent.

Microaggregates that characterize ferralic soils have been hypothesized to have physical, geochemical and/or biological origins. Despite of many studies, the hierarchy between these processes that form microaggregates has seldom been reported. The objective of this work was to study the genesis of microaggregates in a sequence of Ferralic Nitisols developed on Quaternary red clayey sediments and diabase in Piracicaba (SP), Brazil. This issue was tackled by combining optical microscopy, image analysis, scanning electron microscopy and elemental iron quantifications by X-ray fluorescence. Micromorphological investigations showed three different types of microaggregates: (i) oval microaggregates with well sorted quartz grains in their interior; (ii) oval...

External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis

Palavras-chave: Nitisol; Soil genesis; Micromorphology; Scanning electron microscopy.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000300009

Moino Jr.,Alcides; Alves,Sérgio Batista; Lopes,Rogério Biaggioni; Neves,Pedro Manuel Oliveira Janeiro; Pereira,Roberto Manoel; Vieira,Solange Aparecida.

The subterranean termite Heterotermes tenuis is one of the main pests of sugarcane and eucalyptus in Brazil, and the use of entomopathogenic fungi, alone or associated to chemicals, is an efficient and environmentally favorable method for its control. Studies related to the fungal development on these insects are important due to the effect of insect behavior on entomopathogens. The objective of this work was to describe the external development of Beauveria bassiana and Metarhizium anisopliae on H. tenuis using Scanning Electron Microscopy (SEM), determining the duration of the different phases of fungal infection. Two fixation techniques for preparing SEM samples were also evaluated. Worker specimens of H. tenuis were inoculated with a 1 x 10(9) conidia...

Organogenesis from internodal segments of yellow passion fruit

Palavras-chave: Insecta; Isoptera; Microbial control; Scanning electron microscopy.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200010

Biasi,Luiz Antonio; Falco,Maria Cristina; Rodriguez,Adriana P. M.; Mendes,Beatriz M. Januzzi.

Organogenesis induction from internodal segments of yellow passionfruit (Passiflora edulis f. flavicarpa) was obtained in a medium supplemented with 1.0-4.0 mg L-1 benzylaminopurine (BAP). Callus proliferation and adventitious shoot development were analyzed by light and scanning electron microscopy. Callus proliferation and adventitious shoots were observed simultaneously and continuously, with asynchronous development of shoots in the explants. Proliferation of callus was observed in the explant cut ends in all treatments, including the control. The results show that concentrations of 1.0 or 2.0 mg L-1 BAP gave better rate of organogenesis. Higher concentrations were efficient for the induction of adventitious buds and, were, however, detrimental for...

Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion

Palavras-chave: Passiflora edulis f. flavicarpa; Adventitious shoots; Light microscopy; Scanning electron microscopy; Benzylaminopurine.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400010

Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig.

Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered....

Notes on the last instar larva and pupa of Hemiargus hanno (Stoll) (Lepidoptera, Lycaenidae, Polyommatinae)

Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011

Zoologia

Duarte,Marcelo; Almeida,Gisele Luziane de; Casagrande,Mirna Martins; Mielke,Olaf H.H..

Larvae of Hemiargus hanno (Stoll, 1790) (Lepidoptera, Lycaenidae, Polyommatinae) are for the first time reported to be attended under field conditions by ants of the formicine genus Brachymyrmex Mayr, 1868 (Myrmelachistini). Structural features of the last instar larva and pupa of H. hanno are described and illustrated with the aid of scanning electron microscopy (SEM).