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Effect of Flutamide in Mouse Spermatogenesis and on the Function of Seminal Vesicle and Prostate International Journal of Morphology
Bustos-Obregón,Eduardo; Esponda,Pedro; Sarabia,Luis.
The spermatogenesis is regulated by the hypotalamus-hypophysis gonadal axis, and the androgens play a fundamental role in their last stages. The administration of the antiandrogen flutamide interferes with the function of androgen dependent organs (Prostate (P) and Seminal Vesicle (VS)). In this study flutamide (10mg/Kg b.w.) was injected to 10 mice and vehicle to the controls (n=6). The mice were sacrificed at 24 (n=5) and 72 (n=5) hours. In testicular sections the tubular diameter (TD) and height of seminiferous epithelium (EH) were measured. P and VS were macerated, and concentrations of fructose (VS) and zinc (P) were determined. No significant difference exists in the DT among the groups. However, the 72 hrs group showed EH higher than the control (p...
Tipo: Journal article Palavras-chave: Flutamide; Mouse; Spermatogenesis; Seminal vesicles; Prostate.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300008
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Ultrastructural Aspects of Spermatogenesis in Phalloceros caudimaculatus (Teleostei, Poeciliidae) International Journal of Morphology
Vicentini,Carlos Alberto; Muñoz,Mario Esteban; Vicentini,Irene Bastos Franceschini.
The aim of the present study was to analyze the ultrastructural aspects of spermatogenesis in Phalloceros caudimaculatus, during cell proliferation. The parenchyma is organized morphologically as lobular restricted spermatogonial testis. Spermiogenesis in this species is characterized by four morphological stages of development (spermatids S1 through S4). The mature spermatids and spermatozoa heads are situated at the periphery of the cyst surrounded by the cytoplasm of the Sertoli cells. The germ cell's ultrastructure and spermatogenesis in P. caudimaculatus are very similar to that of other poeciliids.
Tipo: Journal article Palavras-chave: Spermatogenesis; Ultrastructure; Poeciliidae; Phalloceros caudimaculatus.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300047
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Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice International Journal of Morphology
Patil,Sharanabasappa A; Patil,Saraswati B.
An attempt to find out the male contraceptive molecule of plant origin, the extracts of seeds of Momordica charantia were tested in male mice. Petroleum ether, chloroform and ethanolic extracts of Momordica charantia were administered at the dose level of 25mg/100gm body weight to the albino mice for 48 days intraperitoneally. All the extracts showed antispermatogenic effect as the number of spermatogonia, spermatocytes, spermatids and spermatozoa were decreased. The increase in the weight of epididymis, prostate gland, seminal vesicle and vas deferens indicates clearly the androgenic property of these extracts. After subjecting to preliminary phytochemical screening ethanol extract showed positive tests for alkaloids, flavonoids, glycosides, phenols,...
Tipo: Journal article Palavras-chave: Momordica charantia Linn; Toxicological; Androgenic; Testis; Spermatogenesis.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400024
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Effect of Prolonged Oral Administration of Aqueous Ficus sycomorus Stem-bark Extract on Testicular Size of Growing Albino Rat International Journal of Morphology
Igbokwe,N. A; Igbokwe,I. O; Sandabe,U. K.
Decoction of Ficus sycomorus stem bark is used to treat infertility involving low sperm counts, but the reproductive risk associated with its use is uncertain. The aqueous stem-bark extract was orally administered at 200-600 mg/kg for 30 days to post-pubertal growing albino rats. Within the period, the live body weights, scrotal diameters and testicular size parameters were estimated. The mean live body weight, scrotal diameter of the control and treated rats significantly (p<0.05) increased as the animals grew, but the treatment did not significantly (p>0.05) affect the parameters. There were no significant (p>0.05) variations in mean testicular size parameters in treated and control rats, and no dose-dependent nor time-dependent effects...
Tipo: Journal article Palavras-chave: Ficus sycomorus extract; Testicular size; Testicular histopathology; Spermatogenesis; Male reproductive risk.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400052
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Angiotensin-converting enzyme inhibitors of Bothrops jararaca snake venom affect the structure of mice seminiferous epithelium J. Venom. Anim. Toxins incl. Trop. Dis.
Alberto-Silva,Carlos; Gilio,Joyce M; Portaro,Fernanda C. V.; Querobino,Samyr M.; Camargo,Antonio C. M..
Background Considering the similarity between the testis-specific isoform of angiotensin-converting enzyme and the C-terminal catalytic domain of somatic ACE as well as the structural and functional variability of its natural inhibitors, known as bradykinin-potentiating peptides (BPPs), the effects of different synthetic peptides, BPP-10c (<ENWPHQIPP), BPP-11e (<EARPPHPPIPP), BPP-AP (<EARPPHPPIPPAP) and captopril were evaluated in the seminiferous epithelium of male mice.Methods The adult animals received either one of the synthetic peptides or captopril (120 nmol/dose per testis) via injection into the testicular parenchyma. After seven days, the mice were sacrificed, and the testes were collected for histopathological evaluation.Results BPP-10c...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B. jararaca; Bradykinin-potentiating peptides; Angiotensin-converting enzyme; Seminiferous epithelium; Spermatogenesis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100338
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Reproductive system and spermatozoa ultrastructure support the phylogenetic proximity of Megadasys and Crasiella (Gastrotricha, Macrodasyida) Naturalis
Guidi, L.; Todaro, M.A.; Ferraguti, M.; Balsamo, M..
The reproductive system and the spermatozoon of Megadasys sterreri from Lanzarote (Canary Islands, Spain) were studied at structural and ultrastructural levels. The species is a simultaneous hermaphrodite with cross-fertilization and shows paired gonads, the male anterior and the female posterior, and both gametes mature in a caudo-cephalic direction. Sperm ducts converge on the midline and open into a ventral common pore. Two sexual accessory organs are present in the caudal trunk. A pipe-like frontal organ lies between the ovaries and the caudal organ, and is composed of a long, thin region connected to a large zone containing mature and degenerating spermatozoa. The cigar-like caudal organ is elongate, bulky and is made of an anterior glandulo-muscular...
Tipo: Article / Letter to the editor Palavras-chave: Cephalodasyidae; Phylogeny; Planodasyidae; Reproductive traits; Spermatogenesis; 42.79; 42.62.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/471589
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Gametogenesis and reproductive cycle of Melanorivulusaff. punctatus (Boulenger, 1895) (Cyprinodontiformes, Rivulidae) in Chapada dos Guimarães, Mato Grosso, Brazil Neotropical Ichthyology
Cassel,Mônica; Mehanna,Mahmoud; Mateus,Lúcia; Ferreira,Adelina.
The comprehension of the reproductive cycle allows to understand which are the morphological changes that develop in the gonad during this interval. Thus, many studies have been undertaken in order to describe and classify the stages of gonadal development and reproductive status of Neotropical fishes. For this purpose, specimens of Melanorivulusaff. punctatus were collected in a permanent dam in Chapada dos Guimarães, Mato Grosso, Brazil. The gonads were prepared for analysis by light microscopy. The oogenesis and spermatogenesis have been described, characterizing the stages of gonadal development, together with assessments of the gonadosomatic ratio, germ cell count and verification of variation of mature oocytes in females. Throughout the year the male...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gonadossomatic ratio; Histology; Oogenesis; Spermatogenesis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100179
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Reproductive cycle of the Neotropical cichlid yellow peacock bass Cichla kelberi: A novel pattern of testicular development Neotropical Ichthyology
Siqueira-Silva,Diógenes Henrique de; Vicentini,Carlos Alberto; Ninhaus-Silveira,Alexandre; Veríssimo-Silveira,Rosicleire.
The present study describes the testicular maturation phases (associating the germ cells development and the morphological changes suffered by the germinal epithelium along the whole year), and the testicular morphology in the yellow peacock bass Cichla kelberi, relating it to other species. For this purpose, 78 specimens were studied according conventional techniques of light microscope. The testes in C. kelberi were classified as unrestricted spermatogonial lobular, an apomorphic characteristic in the recent groups of Teleost. Furthermore, were defined five testicular maturation phases: Preparatory phase; Early Germinal Epithelium Development; Mid Germinal Epithelium Development; Late Germinal Epithelium Development and; Regression. Similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Germinal epithelium; Gonadal maturation; Reproductive phases; Spermatogenesis; Testes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300587
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Annual male reproductive activity and stages of the seminiferous epithelium cycle of the large fruit-eating Artibeus lituratus (Chiroptera: Phyllostomidae) Rev. Bras. Zool.
Notini,Alice A.; Farias,Talita O.; Talamoni,Sônia A.; Godinho,Hugo P..
The large fruit-eating phyllostomid bat, Artibeus lituratus (Olfers, 1818), forearm 69-75 mm, body mass 66-82 g, has a diversified geographic distribution in the Neotropical region. Therefore it is subjected to different climatic conditions that affect its reproduction, leading to different reproductive strategies such as continuous reproduction, seasonal monoestry or seasonal bimodal polyestry. In this study we used morphometric and histological methods to analyze the annual reproductive activity of A. lituratus males in a population living in the Atlantic Forest, Southeastern Brazil. Testis mass, epididymis mass, gonadosomatic index, seminiferous tubule diameter, and Leydig cell nucleus diameter showed no significant differences (p > 0.05) in the two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Neotropical bat; Seminiferous epithelium; Spermatogenesis; Reproduction.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000300195
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Reproductive apparatus and gametogenesis of Lophiosilurus alexandri Steindachner (Pisces, Teleostei, Siluriformes) Zoologia
Barros,Marcelo D. M.; Guimarães-Cruz,Rodrigo J.; Veloso-Júnior,Vanderlei C.; Santos,José E. dos.
The gonad morphology and gametogenesis of Lophiosilurus alexandri Steindachner, 1877 from Santo Antônio river (Minas Gerais, Brazil) were studied through anatomical and histological techniques. Examination of the testis revealed that they were paired, fringed organs with two distinct regions: cranial spermatogenic and caudal spermatogenic and secretory. The cranial region represents ca. 60% of the mature/maturing testis, containing 41 to 73 fringes, while the caudal portion represents ca. 40% and contains 44 to 68 fringes. At the cranial portion the length of the fringes was 3.70 ± 1.70 mm and in the caudal portion was 0.20 ± 0.10 mm. The spermatogenesis was cystic, synchronic, and occurred throughout the whole extension of the seminiferous tubules. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gonads; Morphology; Oogenesis; Pseudopimelodidae; Spermatogenesis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752007000100028
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Puberty in male collared peccary (Pecari tajacu) determined by quantitative analysis of spermatogenic cells Acta Amazonica
Guimarães,Diva Anelie; Cardoso,Deise de Lima; Ferreira,Maria Auxiliadora Pantoja; Albuquerque,Natália Inagaki de.
Biological studies are necessary for the management of wildlife in captivity, and knowledge of reproduction is one of the important features for increasing production. The objective of the research was to determine the age at which male collared peccaries reach puberty. Testicular samples of 15 animals, aged 7 to 16 months, distributed into five groups (G1, G2, G3, G4 and G5) were used. The testes showed considerably increased weight, length and width (p < 0.05) from G1 to G3, whereas, from this group onward, the development of this organ was slower. There was positive correlation (p < 0.001) between the following testicular parameters: weight and length (r = 0.97), weight and width (r = 0.88), length and width (r = 0.92). Regarding the diameter of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reproduction; Testicles; Spermatogenesis; Tayassuidae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672013000100012
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Opioid system manipulation during testicular development: results on sperm production and sertoli cells population - doi: 10.4025/actascibiolsci.v33i2.5940 Biological Sciences
Cajú, Fernanda Mafra; Universidade Federal Rural de Pernambuco; Queiroz, Gian Carlo D'Angelo; Universidade Federal Rural de Pernambuco; Torres, Sandra Maria; Universidade Federal Rural de Pernambuco; Tenório, Bruno Mendes; Universidade Federal Rural de Pernambuco; Silva Júnior, Valdemiro Amaro; Universidade Federal Rural de Pernambuco.
The Sertoli cell has fundamental importance to the development and maintenance of spermatogenesis, as well as it has a directly proportional numerical relationship to sperm production. The proliferative period of this cell in rats occurs between 13 days pre-natal and 21 days pos-natal, when is established the final population in adult animals. The Leydig cell can modulate the Sertoli cell proliferation during fetal and neonatal period through &beta;-endorphin. The manipulation of opioidergic system can promote changes in parameters related to development of nervous, endocrine and reproductive systems. By the way, the main purpose of this present work was to compare the effects of the blockade of opioid receptor blocking in Sertoli cells using...
Tipo: Estudo histomorfológico Palavras-chave: 2.06.00.00-3; 2.06.03.00-2; 2.07.02.00-0 Naltrexone; Testis development; Spermatogenesis; Wistar rats Morfologia; Histologia; Fisiologia de Órgão e Sistemas Naltrexone; Testis development; Spermatogenesis; Wistar rats.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5940
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Experimental exposure to 3-monochloropropane-1,2-diol from the pre-puberty causes damage in sperm production and motility in adulthood Biological Sciences
Vieira, Kátia Cristina Melo Tavares; Favareto, Ana Paula Alves.
3-Monochloropropane-1,2-diol (3-MCPD) is a food contaminant that can be formed during the thermic processing of various foodstuffs. Studies of reproductive toxicology of 3-MCPD are mainly concentrated in the evaluation of possible insults caused by exposure of adult animals. However, the prepuberty might be a period of different susceptibility to chemicals. The aim of this study was to evaluate the effects on reproductive endpoints of the 3-MCPD-exposure prepubertal male rats. Wistar male rats were assigned to 4 groups: control and exposed to 2.5; 5 or 10 mg kg-1 day-1 of 3-MCPD for 30 days by gavage. Testis and epididymis were used for sperm counts and histology analysis. Sertoli cell number and dynamic of the spermatogenesis were evaluated. Sperm were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.06.00.00-3 monochloropropanol; Puberty; Rats; Spermatogenesis; Reproduction. Morfologia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31323
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Effect of pinealectomy, adrenalectomy, pinealectomy plus adrenalectomy upon the quantification of spermatogenic cells of adult rats Arq. Bras. Med. Vet. Zootec.
Castro,A.C.S.; Queiroz,G.F.; Nogueira,J.C.; Coimbra,C.C.; Reis,A.M.; Marubayashi,U.; Yamasaki,K..
The objectives of this study were to evaluate the effects of pinealectomy, adrenalectomy and pinealectomy-adrenalectomy upon the quantification of spermatogenic cells of rats. As such, 32 adult Wistar rats with a mean body weight of 331.7± 15.5g were assigned into one of the following treatments: (a) a sham-operated control group, consisting of nine animals; (b) ten pinealectomized animals; (c) seven adrenalectomized animals and (d) six pinealectomized plus adrenalectomized animals. No significant differences were observed between groups for the following parameters: body, testes, prostate and seminal vesicle weights, seminiferous tubular diameter, number of cells per seminiferous tubular cross sections (primary spermatocytes at pachytene, round...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Pinealectomy; Adrenalectomy; Rat.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300008
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Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere Biocell
Ferreira,Adelina; Dolder,Heidi.
Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the pro-acrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ultrastructure; Sperm; Spermatogenesis; Lizard; Tropiduridae.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300006
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Seasonal testicular changes in Dendropsophus minutus Peters, 1872 (Anura, Hylidae) Biocell
Ferreira,Adelina; Mehanna,Mahmoud.
The reproductive cycle in anurans may be either continuous or discontinuous. These differences may be connected to seasonal climate changes and/or to anthropic activity. Forty adult male individuals of the Dendropsophus minutus species were collected during one year, in the municipality of Chapada dos Guimarães (Mato Grosso, Brazil). The testicles were studied under light and transmission electron microscopy. No variations were observed when the diameter of the seminiferous tubules and the thickness of the interstitial tissue were studied. However, changes in spermatogenesis were conspicuous and indicated that the reproductive cycle of D. minutus in Chapada dos Guimarães is discontinuous and seems related to variations in air temperature and rainfall.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Reproduction; Anura; Ultrastructure; Chapada dos Guimarães.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200002
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Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil Biocell
Ferreira,Adelina; Mehanna,Mahmoud; Prado,Cynthia P. A..
The spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Male; Amphibia; Climatic influences.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200004
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Morphological study of the spermatogenesis in the teleost Piaractus mesopotamicus Biocell
da Cruz-Landim,Carminda; Camargo Abdalla,Fábio; da Cruz-Höfling,Maria Alice.
The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type in fishes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Spermatogenesis; Spermatogonia; Spermiogenesis; Teleostei.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300003
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An integrative view to structure, function, ontogeny and phylogenetical significance of the male genital system in Pomacea canaliculata (Caenogastropoda, Ampullariidae) Biocell
Gamarra-Luques,C.; Winik,B.C.; Vega,I.A.; Albrecht,E.A.; Catalan,N.M.; Castro-Vazquez,A..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Steroids; Peptides; Imposex; Xenobiotics.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200012
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Testosterone production and spermatogenic damage induced by organophosphorate pesticides Biocell
Contreras,H.R.; Paredes,V.; Urquieta,B.; Del Valle,L.; Bustos-Obregón,E..
ParathionÒ is an organophosphorate pesticide amply used in agriculture. Many alterations induced by organophosphorate pesticides have been described, such as: cytogenetic alterations in germinal cells, oligozoospermia and teratozoospermia in the mouse. The effect of ParathionÒ, both pure (PP) and commercial (PC), on mouse interstitial cell testosterone production was evaluated in vivo and in vitro. Male mice were intraperitoneally injected with a single dose of 1/3 LD50 of ParathionÒ, both PP and PC. The animals were sacrificed at 1, 8 and 40 days post injection to evaluate the impact of disrupting testosterone production on spermatogonia, spermatocytes and elongated spermatids. The plasma testosterone was assayed by standard radioimmunoanalysis. The same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Steroidogenesis; Spermatogenesis; Pesticide; ParathionÒ.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300002
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