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| Registros recuperados: 33 | |
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| Costa,Fábio L. S.; Lima,Maria E. De; Pimenta,Adriano C.; Figueiredo,Suely G.; Kalapothakis,Evanguedes; Salas,Carlos E.. |
| Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDNAs; EST; Glands; Lectin; Scorpionfish; Toxins. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400871 |
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| Weisman, R.; Baden, D.G.; Blythe, D.G.; Fleming, L.E.; Bean, J.A.. |
| In addition to increased seafood consumption and tourism, recent studies link global climate change with an apparent increasing incidence of the Marine Seafood Toxin diseases. However, the epidemiology of the human diseases caused by the harmful marine phytoplankton is still in its infancy. In general, the epidemiology of these diseases has consisted of case reports of acute illness, sometimes as epidemic outbreaks, associated with the ingestion of suspicious seafood. Furthermore, even these outbreaks are highly under-reported, especially in poorer countries and in traditionally non-endemic areas. True incidence data are not available due to the lack of disease and exposure biomarkers in humans, as well as the global lack of routine exposure and disease... |
| Tipo: Report |
Palavras-chave: Toxins; Diseases. |
| Ano: 1998 |
URL: http://hdl.handle.net/1834/758 |
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| Pitcher, G.C.; Roesler, C.S.; Etheridge, S.M.. |
| Abalone were grown: on diets of 1) artificial feed, 2) kelp, their in situ food source, which contained saxitoxin (STX) derivatives, and 3) in filtered seawater without a food source, to investigate the depuration and transformation of toxins under feeding and starving conditions. The abalone were toxic at the start of each treatment (~160 µg STX eq 100 g-1 tissue). They depurated at a rate of 6.27 µg STX eq 100 g-1 tissue d-1 over the 2 week incubation period when fed artificial feed; however, no depuration was observed in starved or kelp-fed animals. Toxin transformations occurred in abalone for each treatment. Toxin derivatives decreased in organisms fed artificial feed, and no B1 was detected during this treatment. Toxin composition was not... |
| Tipo: Working Paper |
Palavras-chave: Toxins. |
| Ano: 2003 |
URL: http://hdl.handle.net/1834/376 |
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| Taffarel,Loreno Egidio; Mesquita,Eduardo Eustáquio; Castagnara,Deise Dalazen; Costa,Patrícia Barcellos; Neres,Marcela Abbado; Horn,Marcelina Bottoni; Oliveira,Paulo Sérgio Rabello de; Meinerz,Cristiane Claudia. |
| The objective of this study was to estimate the dehydration curve and occurrence of fungi and mycotoxins in Tifton 85 hay. The experimental design was randomized blocks in split plots with four replications and five levels of nitrogen (0, 25, 50, 75 and 100 kg ha-1) in the form of urea, two regrowth ages (28 and 35 days) and drying hay in the sun and in shed. After harvesting, the grass was sampled four times (0, 8, 23 and 32 hours) to dry in the sun and twelve times (0, 3, 18, 27, 42, 51, 66, 75, 90, 99, 114 and 123 hours) for drying in shed, turned over daily. The fungi were identified after seeding and growth in three steps of haymaking (cutting, baling and after 30 days of storage). To verify the presence of mycotoxins, 20 samples, composed of hay... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contaminants; Dry matter; Forage conservation; Microorganisms; Toxins. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000600003 |
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| Onofre,Sideney Becker; Riveros Gonzalez,Raul; Messias,Cláudio Luiz; Azevedo,João Lúcio; Barros,Neiva Monteiro de. |
| The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticarsia gemmatalis; Nomuraea rileyi; Integrated control; Secondary metabolite; Toxins. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004 |
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| Vasconcelos,V.M.. |
| Toxic cyanobacteria are common in Portuguese freshwaters and the most common toxins are microcystins. The occurrence of microcystin-LR (MCYST-LR) has been reported since 1990 and a significant number of water reservoirs that are used for drinking water attain high levels of this toxin. Aquatic animals that live in eutrophic freshwater ecosystems may be killed by microcystins but in many cases the toxicity is sublethal and so the animals can survive long enough to accumulate the toxins and transfer them along the food chain. Among these, edible mollusks, fish and crayfish are especially important because they are harvested and sold for human consumption. Mussels that live in estuarine waters and rivers where toxic blooms occur may accumulate toxins without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyanobacteria; Toxins; Risk; Human health; Microcystins; Accumulation. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300001 |
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| The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown... |
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Palavras-chave: Liposomes; Neutralizing antibodies; Tityus serrulatus; Immunization; Toxins. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010 |
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| Stojanovic,Predrag; Kocic,Branislava; Stojanovic,Miodrag; Miljkovic-Selimovic,Biljana; Tasic,Suzana; Miladinovic-Tasic,Natasa; Babic,Tatjana. |
| The aim of this study was to fortify the clinical importance and representation of toxigenic and non-toxigenic Clostridium difficile isolated from stool samples of hospitalized patients. This survey included 80 hospitalized patients with diarrhea and positive findings of Clostridium difficile in stool samples, and 100 hospitalized patients with formed stool as a control group. Bacteriological examination of a stool samples was conducted using standard microbiological methods. Stool sample were inoculated directly on nutrient media for bacterial cultivation (blood agar using 5% sheep blood, Endo agar, selective Salmonella Shigella agar, Selenite-F broth, CIN agar and Skirrow's medium), and to selective cycloserine-cefoxitin-fructose agar (CCFA) (Biomedics,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium difficile; Diarrhea; Toxins. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100023 |
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| Vidotto,Marilda C.; Lima,Natália C.S. de; Fritzen,Juliana T.T.; Freitas,Júlio C. de; Venâncio,Emerson J.; Ono,Mario A.. |
| Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine Escherichia coli; Virulence genes; Postweaning diarrhea; Toxins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100035 |
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| Ferreira,Thais Sebastiana Porfida; Moreno,Andrea Micke; Almeida,Renata Rodrigues de; Gomes,Cleise Ribeiro; Gobbi,Debora Dirani Sena de; Filsner,Pedro Henrique Nogueira de Lima; Moreno,Marina. |
| Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Swine; Slaughterhouse; PFGE; Carcass; Toxins. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020 |
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| Registros recuperados: 33 | |
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