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| Registros recuperados: 104 | |
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| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行. |
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Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice. |
| Ano: 1996 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254 |
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| Kato, Kentaro; Sugi, Tatsuki; Takemae, Hitoshi; Takano, Ryo; Gong, Haiyan; Ishiwa, Akiko; Horimoto, Taisuke; Akashi, Hiroomi. |
| Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the... |
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Palavras-chave: Calcium calmodulin-dependent protein kinase homolog; GAP45; Phosphorylation; T. gondii CaMK-related kinase; Toxoplasma gondii. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4447 |
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| Ibrahim, H. M.; Nishikawa, Y. |
| The current study investigated the impact of the CCR5, TLR2 and TLR11 on production of nitric oxide (NO), IL-6 and IL-12 and growth of T. gondii, high virulent RH strain and avirulent PLK strain. All examined knockout macrophages infected with PLK strain produced significant lower levels of IL-12. On the other hand, TLR2 and TLR11 macrophages infected with RH strain produced significantly reduced levels of IL-12 as compared to wild-type macrophages. TLR2 and TLR11 macrophages infected with PLK strain significantly inhibited the production of IL-6 when compared to wild-type macrophages. Interestingly, significant reduction in the IL-6 and NO production was observed in TLR2 macrophages infected with PLK strain as compared to wild-type macrophages or to the... |
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Palavras-chave: Toxoplasma gondii; CCR5; TLR2; TLR11. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4495 |
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| Kamyingkird, K.; Goo, Y.K.; Cao, S.; Adjou Moumouni, P.K.; Aboge, G.O.; Yamagishi, J.; Terkawi, M.A.; Masatani, T.; Yu, L.; Nishikawa, N.; Xuan, X.. |
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Palavras-chave: Aspartic protease; Toxoplasma gondii. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4093 |
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| Salman, Doaa; Okuda, Liria Hiromi; Ueno, Akio; Dautu, George; Zhang, Feifei; Igarashi, Makoto. |
| Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA).... |
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Palavras-chave: T. gondii oocyst wall protein; Toxoplasma gondii. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4541 |
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| Kato, Kentaro; Murata, Yuho; Horiuchi, Noriyuki; Inomata, Atsuko; Terkawi, Mohamad Alaa; Ishiwa, Akiko; Ogawa, Yohsuke; Fukumoto, Shinya; Matsuhisa, Fumikazu; Koyama, Kenji. |
| Background: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. Methods: Fourteen-day-old male pigs (n = 10) were infected with T.... |
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Palavras-chave: Dextran sulfate; Intermediate host; Pig; Public health; Toxoplasma gondii. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4446 |
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| Mahmoud, Motamed Elsayed; Fereig, Ragab; Nishikawa, Yoshifumi. |
| Toxoplasma gondii is a pathogen relevant to psychiatric disorders. We recently showed that reactivation of chronic T. gondii infection induced depression-like behaviors in mice. Furthermore, it has been hypothesized that depression-like behaviors are mediated via a host defense mechanism against invading pathogens; proximate mechanisms of this behavioral hypothesis remain unclear. In the present study, we investigate the contribution of indoleamine 2,3-dioxygenase (IDO), inflammation, and interferon gamma (IFN-gamma) to anhedonic and despair-related behaviors in T. gondii-infected mice by using sucrose preference and forced-swim tests, respectively. First, we confirmed that BALB/c mice exhibited both sickness and depression-like behaviors during acute... |
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Palavras-chave: Toxoplasma gondii; 1-methyl tryptophan; Minocycline; Interferon gamma; Sickness behavior; Anhedonic behavior; Despair-like behavior. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4507 |
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| Dautu, George; Munyaka, Biscah; Carmen, Gabriella; Zhang, Guohong; Omata, Yoshitaka; Xuan, Xuenan; Igarashi, Makoto. |
| A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific... |
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Palavras-chave: Toxoplasma gondii; DNA; Deoxyribonucleic acid; DNA immunization; MIC2; Microneme protein-2; M2AP; MIC2 associated protein; AMA1; Apical membrane antigen-1; BAG1; Bradyzoite antigen-1; IgG; Immunoglobulin G. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1031 |
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| Fereig, Ragab M.; Nishikawa, Yoshifumi. |
| Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the... |
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Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569 |
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| Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇. |
| Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p < 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2... |
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Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236 |
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| AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男. |
| Toxoplasma gondii is an important zoonotic parasite. The diagnosis of infection in animals is an important tool to prevent human infection. In the present study, Toxoplasma gondii was diagnosed by using the modified agglutination test (MAT) in ducks from Behera Governorate, Egypt. The prevalence of Toxoplasma gondii was 13.9%. The prevalence of Toxoplasma gondii was the highest in the Native breed (17.65%) and in 6-8-months age group (19.4%). End-point titer of 1:25, 1:50, and 1:100 were recorded in 61.9%, 19.05%, and 19.05% of the positive samples, respectively. Duck meat is one source of the Toxoplasma gondii infection for human in Egypt. |
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Palavras-chave: Toxoplasma gondii; Modified agglutination test (MAT); Ducks; Egypt. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3128 |
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| Fereig, Ragab M.; Mahmoud, Hassan Y.A.H.; Mohamed, Samy G.A.; AbouLaila, Mahmoud Rezk; Abdel-Wahab, Azza; Osman, Salama Ahmed; Zidan, Sherif Abdallah; El-Khodary, Sabry A.; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. |
| Toxoplasmosis is a cosmopolitan protozoan disease that has been recorded in a wide range of vertebrate hosts, including humans. In response to the paucity of available data, this study was undertaken to comprehensively establish the seroprevalence of Toxoplasma gondii among various farm animals in different localities of Egypt. The latex agglutination test and TgGRA7-based enzyme-linked immunosorbent assay were used to screen the investigated animals for anti-T. gondii IgG antibodies. When only samples with simultaneously positive results for both the latex agglutination test and the TgGRA7-based ELISA were considered positive, 174 (26.7%) of 652 serum samples from different animals were seropositive. The prevalence of antibodies according to species was:... |
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Palavras-chave: Egypt; Seroprevalence; Toxoplasma gondii; Toxoplasmosis. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4545 |
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| Registros recuperados: 104 | |
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