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Registros recuperados: 25
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Effects of nickel fertilization on soybean growth in tropical soils Bragantia
Levy,Camila de Carvalho Braga; Mellis,Estêvão Vicari; Murrer,Marina Krey; Inglés,Carlos Repiso; Daynes,Cathal N.; Cavalli,Edilson; Chiba,Marcio Koiti.
ABSTRACT Although nickel (Ni) is recognized as plant nutrient since the turn of the 21st century, uncertainty remains about its optimum application rates and forms. This paper focuses on Ni application in soils, relating to its effect as a plant micronutrient. Specifically, the effect of Ni on the activity of urease in soybean plants is examined. The effects of applying four Ni rates in two different soil types were tested. A full factorial 2 × 4 blocked design experiment was established under controlled conditions. Ni rates (0, 0.25, 0.5 and1.0 mg of Ni·kg–1 of soil) were applied in two soils with contrasting clay contents. The addition of Ni increased the urease activity in soybean plants but was affected by soil textural differences. The highest urease...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Clay; Sand; Oxisol; Glycine max.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000300432
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Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk BABT
Kaur,Hardeep; Kumar,Sachin; Verma,Neelam.
The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 -...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Urease; Hydrosol- gel immobilization; Lead; Milk; Masking agent.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613
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Characteristics of Immobilized Urease on Grafted Alginate Bead Systems BABT
Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H..
This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Immobilization; Kinetic; Reusability; Storage stability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
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Ureases display biological effects independent of enzymatic activity: Is there a connection to diseases caused by urease-producing bacteria? BJMBR
Olivera-Severo,D.; Wassermann,G.E.; Carlini,C.R..
Ureases are enzymes from plants, fungi and bacteria that catalyze the hydrolysis of urea to form ammonia and carbon dioxide. While fungal and plant ureases are homo-oligomers of 90-kDa subunits, bacterial ureases are multimers of two or three subunit complexes. We showed that some isoforms of jack bean urease, canatoxin and the classical urease, bind to glycoconjugates and induce platelet aggregation. Canatoxin also promotes release of histamine from mast cells, insulin from pancreatic cells and neurotransmitters from brain synaptosomes. In vivo it induces rat paw edema and neutrophil chemotaxis. These effects are independent of ureolytic activity and require activation of eicosanoid metabolism and calcium channels. Helicobacter pylori, a Gram-negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Canatoxin; Helicobacter pylori; Inflammation; Neutrophils; Eicosanoids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700002
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Biomineralization processes of calcite induced by bacteria isolated from marine sediments BJM
Wei,Shiping; Cui,Hongpeng; Jiang,Zhenglong; Liu,Hao; He,Hao; Fang,Nianqiao.
Biomineralization is a known natural phenomenon associated with a wide range of bacterial species. Bacterial-induced calcium carbonate precipitation by marine isolates was investigated in this study. Three genera of ureolytic bacteria, Sporosarcina sp., Bacillus sp. and Brevundimonas sp. were observed to precipitate calcium carbonate minerals. Of these species, Sporosarcina sp. dominated the cultured isolates. B. lentus CP28 generated higher urease activity and facilitated more efficient precipitation of calcium carbonate at 3.24 ± 0.25 × 10−4 mg/cell. X-ray diffraction indicated that the dominant calcium carbonate phase was calcite. Scanning electron microscopy showed that morphologies of the minerals were dominated by cubic, rhombic and polygonal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium carbonate precipitation; Calcite; Marine bacteria; Urease.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200455
Registros recuperados: 25
Primeira ... 12 ... Última
 

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