SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Ma,Zhong-Rui; Shi,Li-Jun; Deng,Ming-Hong.

Insomnia is highly prevalent in children and adolescents. However, the efficacy of cognitive behavioral therapy for insomnia (CBT-i) in children and adolescents remains controversial. Therefore, this systematic review and meta-analysis aimed to assess the efficacy of CBT-i in children and adolescents. We conducted a search of PubMed, EMBASE, the Cochrane Central Register of Controlled Trials, CINAHL, and PsycINFO to select primary studies evaluating CBT-i in children and adolescents that were primarily diagnosed through standardized diagnostic criteria. The primary outcomes of the meta-analysis included sleep onset latency (SOL), wake after sleep onset (WASO), total sleep time (TST), and sleep efficiency (SE%). Six randomized controlled trials and four...

Conditioned medium from activated spleen cells supports the survival of rat retinal cells in vitro

Palavras-chave: Insomnia; Cognitive behavioral therapy; CBT-i; Child; Adolescent; Meta-analysis.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600616

Sholl-Franco,A.; Araujo,E.G..

Cytokines are a heterogeneous group of molecules that have been associated with several functions in the nervous system, such as survival and differentiation of neuronal and glial cells. In the present study, we demonstrated that conditioned medium from spleen cells activated with concanavalin A increased neuritogenesis and survival of retinal cells, as measured by biochemical and morphological criteria. Our data showed that conditioned medium induced a five-fold increase in the amount of protein after 120 h in vitro. This effect was not inhibited by the blockade of voltage-dependent L-type calcium channels with 5.0 µM nifedipine. However, the use of an intracellular calcium chelator (15.0 µM BAPTA-AM) inhibited this effect. Our results support the idea...

Natural preference of zebrafish (Danio rerio) for a dark environment

Palavras-chave: Cytokines; Growth factor; Neuroimmunology; Retina; Development.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100006

Serra,E.L.; Medalha,C.C.; Mattioli,R..

The zebrafish (Danio rerio) has been used as a model in neuroscience but knowledge about its behavior is limited. The aim of this study was to determine the preference of this fish species for a dark or light environment. Initially we used a place preference test and in a second experiment we applied an exit latency test. A two-chamber aquarium was used for the preference test. The aquarium consisted of a black chamber and a white chamber. In the first experiment the animal was placed in the aquarium and the time spent in the two compartments was recorded for 10 min. More time was spent in the black compartment (Wilcoxon matched-pairs signed-rank test, T = 7, N1 = N2 = 18, P = 0.0001). In the second experiment the animal was placed in the black or white...

Palavras-chave: Zebrafish Danio rerio behavior preference dark; Light.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200016

Correction

Molluscicidal activity of Punica granatum bark and Canna indica root

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700014

Tripathi,S.M.; Singh,D.K..

The molluscicidal activity of Punica granatum Linn. (Punicaceae) and Canna indica Linn. (Cannaceae) against the snail Lymnaea acuminata was studied. The molluscicidal activity of P. granatum bark and C. indica root was found to be both time and dose dependent. The toxicity of P. granatum bark was more pronounced than that of C. indica. The 24 h LC50 of the column-purified root of C. indica was 6.54 mg/l whereas that of the column-purified bark of P. granatum was 4.39 mg/l. The ethanol extract of P. granatum (24 h LC50: 22.42 mg/l) was more effective than the ethanol extract of C. indica (24 h LC50: 55.65 mg/l) in killing the test animals. P. granatum and C. indica may be used as potent molluscicides since the concentrations used to kill the snails were not...

Acute effect of passive cycle-ergometry and functional electrical stimulation on nitrosative stress and inflammatory cytokines in mechanically ventilated critically ill patients: a randomized controlled trial

Palavras-chave: Punica granatum; Canna indica; Plant molluscicide; Lymnaea acuminata.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100014

França,E.E.T.; Gomes,J.P.V.; De Lira,J.M.B.; Amaral,T.C.N.; Vilaça,A.F.; Paiva Júnior,M.D.S.; Elihimas Júnior,U.F.; Correia Júnior,M.A.V.; Forgiarini Júnior,L.A.; Costa,M.J.C.; Andrade,M.A.; Ribeiro,L.C.; De Castro,C.M.M.B..

Early mobilization is beneficial for critically ill patients because it reduces muscle weakness acquired in intensive care units. The objective of this study was to assess the effect of functional electrical stimulation (FES) and passive cycle ergometry (PCE) on the nitrous stress and inflammatory cytometry in critically ill patients. This was a controlled, randomized, open clinical trial carried out in a 16-bed intensive care unit. The patients were randomized into four groups: Control group (n=10), did not undergo any therapeutic intervention during the study; PCE group (n=9), lower-limb PCE for 30 cycles/min for 20 min; FES group (n=9), electrical stimulation of quadriceps muscle for 20 min; and FES with PCE group (n=7), patients underwent PCE and FES,...

Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence

Palavras-chave: Cytokines; Nitrosative stress; Intensive care unit; Physiotherapy.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400605

Kettlun,A.M.; Espinosa,V.; Zanocco,A.; Valenzuela,M.A..

Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the different kinetic behavior of the Pimpernel (ATPase/ADPase = 10) and Desirée (ATPase/ADPase = 1) isoenzymes, the nucleotide-binding site of these apyrases was explored using the intrinsic fluorescence of tryptophan. The intrinsic fluorescence of the two apyrases was slightly different. The maximum emission wavelengths of the Desirée and Pimpernel enzymes were 336 and 340 nm, respectively, suggesting small...

EEG spike source localization before and after surgery for temporal lobe epilepsy: a BOLD EEG-fMRI and independent component analysis study

Palavras-chave: Potato apyrase; ATP-diphosphohydrolase; Intrinsic fluorescence; Quenching; Desirée and Pimpernel isoapyrases.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700001

Sercheli,M.S.; Bilevicius,E.; Alessio,A.; Ozelo,H.; Pereira,F.R.S.; Rondina,J.M.; Cendes,F.; Covolan,R.J.M..

Simultaneous measurements of EEG-functional magnetic resonance imaging (fMRI) combine the high temporal resolution of EEG with the distinctive spatial resolution of fMRI. The purpose of this EEG-fMRI study was to search for hemodynamic responses (blood oxygen level-dependent - BOLD responses) associated with interictal activity in a case of right mesial temporal lobe epilepsy before and after a successful selective amygdalohippocampectomy. Therefore, the study found the epileptogenic source by this noninvasive imaging technique and compared the results after removing the atrophied hippocampus. Additionally, the present study investigated the effectiveness of two different ways of localizing epileptiform spike sources, i.e., BOLD contrast and independent...

Application of the differential display RT-PCR strategy for the identification of inflammation-related mouse genes

Palavras-chave: Interictal epileptiform spike; Epilepsy surgery; EEG-fMRI; Temporal lobe epilepsy; ICA; Dipole model.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600017

Silva,A.M.; Pires,E.G.; Abrantes,E.F.; Ferreira,L.R.P.; Gazzinelli,R.T.; Reis,L.F.L..

The inflammatory response elicited by various stimuli such as microbial products or cytokines is determined by differences in the pattern of cellular gene expression. We have used the differential display RT-PCR (DDRT-PCR) strategy to identify mRNAs that are differentially expressed in various murine cell types stimulated with pro-inflammatory cytokines, microbial products or anti-inflammatory drugs. Mouse embryonic fibroblasts (MEFs) were treated with IFNs, TNF, or sodium salicylate. Also, peritoneal macrophages from C3H/Hej mice were stimulated with T. cruzi-derived GPI-mucin and/or IFN-g. After DDRT-PCR, various cDNA fragments that were differentially represented on the sequencing gel were recovered, cloned and...

Dialysis, time and death: comparisons of two consecutive decades among patients treated at the same Brazilian dialysis center

Palavras-chave: DDRT-PCR; Gene expression; Inflammation; TNF; Interferon.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000700008

De-Lima,J.J.G.; da-Fonseca,J.A.; Godoy,A.D..

The survival of hemodialysis patients is likely to be influenced not only by well-known risk factors like age and comorbidity, but also by changes in dialysis technology and practices accumulated along time. We compared the survival curves, dialysis routines and some risk factors of two groups of patients admitted to a Brazilian maintenance hemodialysis program during two consecutive decades: March 1977 to December 1986 (group 1, N = 162) and January 1987 to June 1997 (group 2, N = 237). The median treatment time was 22 months (range 1-198). Survival curves were constructed using the Kaplan-Meier method and compared using the log-rank method. The Cox proportional hazard regression model was used to investigate the more important variables associated with...

Effect of frequency of static stretching on flexibility, hamstring tightness and electromyographic activity

Palavras-chave: Chronic renal failure; Hemodialysis; Kaplan-Meier survival curves; Risk factors.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300007

Marques,A.P.; Vasconcelos,A.A.P.; Cabral,C.M.N.; Sacco,I.C.N..

We compared the effect of the number of weekly repetitions of a static stretching program on the flexibility, hamstring tightness and electromyographic activity of the hamstring and of the triceps surae muscles. Thirty-one healthy subjects with hamstring tightness, defined as the inability to perform total knee extension, and shortened triceps surae, defined by a tibiotarsal angle wider than 90° during trunk flexion, were divided into three groups: G1 performed the stretching exercises once a week; G2, three times a week, and G3, five times a week. The parameters were determined before and after the stretching program. Flexibility improved in all groups after intervention, from 7.65 ± 10.38 to 3.67 ± 12.08 in G1, from 10.73 ± 12.07 to 0.77 ± 10.45 in G2,...

Influence of renal denervation on blood pressure, sodium and water excretion in acute total obstructive apnea in rats

Palavras-chave: Electromyography; Flexibility; Muscle stretching exercises; Range of motion.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000012

Franquini,J.V.M.; Medeiros,A.R.S.; Andrade,T.U.; Araújo,M.T.M.; Moysés,M.R.; Abreu,G.R.; Vasquez,E.C.; Bissoli,N.S..

Obstructive apnea (OA) can exert significant effects on renal sympathetic nerve activity (RSNA) and hemodynamic parameters. The present study focuses on the modulatory actions of RSNA on OA-induced sodium and water retention. The experiments were performed in renal-denervated rats (D; N = 9), which were compared to sham (S; N = 9) rats. Mean arterial pressure (MAP) and heart rate (HR) were assessed via an intrafemoral catheter. A catheter was inserted into the bladder for urinary measurements. OA episodes were induced via occlusion of the catheter inserted into the trachea. After an equilibration period, OA was induced for 20 s every 2 min and the changes in urine, MAP, HR and RSNA were recorded. Renal denervation did not alter resting MAP (S: 113 ± 4 vs...

Single nucleotide polymorphisms predisposing to asthma in children of Mauritian Indian and Chinese Han ethnicity

Palavras-chave: Obstructive apnea; Renal nerve; Natriuresis; Diuresis.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200010

Ramphul,K.; Lv,J.; Hua,L.; Liu,Q.H.; Fang,D.Z.; Ji,R.X.; Bao,Y.X..

Our objective was to investigate the distributions of six single nucleotide polymorphisms (SNPs) MS4A2 E237G, MS4A2 C-109T, ADRB2 R16G, IL4RA I75V,IL4 C-590T, and IL13 C1923T in Mauritian Indian and Chinese Han children with asthma. This case-control association study enrolled 382 unrelated Mauritian Indian children, 193 with asthma and 189 healthy controls, and 384 unrelated Chinese Han children, 192 with asthma and 192 healthy controls. The SNP loci were genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism for the Chinese Han samples and TaqMan real-time quantitative PCR for the Mauritian Indian samples. In the Mauritian Indian children, there was a significant difference in the distribution of IL13 C1923T between the...

Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans

Palavras-chave: Asthma; Single nucleotide polymorphisms; Mauritian Indian; Chinese Han.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500394

Cavalheiro,R.A.; Fortes,F.; Borecký,J.; Faustinoni,V.C.; Schreiber,A.Z..

The respiration, membrane potential (Dy), and oxidative phosphorylation of mitochondria in situ were determined in spheroplasts obtained from Candida albicans control strain ATCC 90028 by lyticase treatment. Mitochondria in situ were able to phosphorylate externally added ADP (200 µM) in the presence of 0.05% BSA. Mitochondria in situ generated and sustained stable mitochondrial Dy respiring on 5 mM NAD-linked substrates, 5 mM succinate, or 100 µM N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride plus 1 mM ascorbate. Rotenone (4 µM) inhibited respiration by 30% and 2 µM antimycin A or myxothiazole and 1 mM cyanide inhibited it by 85%. Cyanide-insensitive respiration was...

JAK/STAT-deficient cell lines

Palavras-chave: Candida albicans spheroplasts; Mitochondria; Respiratory chain; Mitochondrial membrane potential; Uncoupling protein.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000003

Bonjardim,C.A..

Mutant cell lines B3 and B10, which are unresponsive to both interferon (IFN)-alpha and IFN-gamma, and line B9, which does not respond to IFN-gamma stimulation, are described. The mutants were submitted to fluorescence-activated cell sorting (FACS) from a cellular pool, which was obtained from the parental cell line 2C4 after several rounds of mutagenesis. The unresponsiveness to IFN stimulation was observed both in terms of expression of cell surface markers (CD2, class I and II HLAs) and mRNA expression of IFN-stimulated genes (2'-5' oligoadenylate synthetase (OAS), 9-27, and guanylate binding protein (GBP)). Genetic crossing of B3, B9 and B10 with U3 (STAT1-), gamma2a (JAK2-) and U4 (JAK1-) mutants, respectively, did not restore IFN responsiveness to...

Chronic aerobic exercise associated to low-dose L-NAME improves contractility without changing calcium handling in rat cardiomyocytes

Palavras-chave: Interferons; JAK/STAT; Molecular genetics; FACS analysis; RNase protection; Transcriptional activation.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100004

Luchi,T.C.; Coelho,P.M.; Cordeiro,J.P.; Assis,A.L.E.M.; Nogueira,B.V.; Marques,V.B.; dos Santos,L.; Lima-Leopoldo,A.P.; Lunz,W.; Leopoldo,A.S..

Nitric oxide (NO) inhibition by high-dose NG-nitro-L-arginine methyl ester (L-NAME) is associated with several detrimental effects on the cardiovascular system. However, low-dose L-NAME increases NO synthesis, which in turn induces physiological cardiovascular benefits, probably by activating a protective negative feedback mechanism. Aerobic exercise, likewise, improves several cardiovascular functions in healthy hearts, but its effects are not known when chronically associated with low-dose L-NAME. Thus, we tested whether the association between low-dose L-NAME administration and chronic aerobic exercise promotes beneficial effects to the cardiovascular system, evaluating the cardiac remodeling process. Male Wistar rats were randomly assigned to control...

Inflammatory response of the spinal cord to multiple episodes of blood-brain barrier disruption and toxic demyelination in Wistar rats

Palavras-chave: Nitric oxide; Aerobic exercise; Cardiac remodeling; Cardiomyocyte; Calcium.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300611

Fernandes,C.G.; Graça,D.L.; Pereira,L.A.V.D..

Multiple episodes of blood-brain barrier disruption were induced by sequential intraspinal injections of ethidium bromide. In addition to the barrier disruption, there was toxic demyelination and exposure of myelin components to the immune system. Twenty-seven 3-month-old Wistar rats received 2, 3 or 4 injections of 1 µl of either 0.1% ethidium bromide in normal saline (19 rats) or 0.9% saline (8 rats) at different levels of the spinal cord. The time intervals between the injections ranged from 28 to 42 days. Ten days after the last injection, all rats were perfused with 2.5% glutaraldehyde. The spinal sections were evaluated macroscopically and by light and transmission electron microscopy. All the lesions demonstrated a mononuclear phagocytic infiltrate...

Can the fractal dimension be applied for the early diagnosis of non-proliferative diabetic retinopathy?

Palavras-chave: Lymphocytes; Multiple sclerosis; Central nervous system; Blood-brain barrier; Ethidium bromide.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700008

Kunicki,A.C.B.; Oliveira,A.J.; Mendonça,M.B.M.; Barbosa,C.T.F.; Nogueira,R.A..

The fractal dimension has been employed as a useful parameter in the diagnosis of retinal disease. Avakian et al. (Curr Eye Res 2002; 24: 274-280), comparing the vascular pattern of normal patients with mild to moderate non-proliferative diabetic retinopathy (NPDR), found a significant difference between them only in the macular region. This significant difference in the box-counting fractal dimension of the macular region between normal and mild NPDR patients has been proposed as a method of precocious diagnosis of NPDR. The aim of the present study was to determine if fractal dimensions can really be used as a parameter for the early diagnosis of NPDR. Box-counting and information fractal dimensions were used to parameterize the vascular pattern of the...

Specific insulin and proinsulin secretion in glucokinase-deficient individuals

Palavras-chave: Box-counting; Fractal dimension; Information fractal dimension; Retina; Vascularization; Non-proliferative diabetic retinopathy.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000009

Pardini,V.C.; Velho,G.; Reis,R.; Purisch,S.; Blanché,H.; Vieira,J.G.H.; Moisés,R.C.S..

Glucokinase (GCK) is an enzyme that regulates insulin secretion, keeping glucose levels within a narrow range. Mutations in the glucokinase gene cause a rare form of diabetes called maturity-onset diabetes of the young (MODY). An early onset (less than 25 years), autosomal dominant inheritance and low insulin secretion stimulated by glucose characterize MODY patients. Specific insulin and proinsulin were measured in serum by immunofluorimetric assays (IFMA) during a 75-g oral glucose tolerance test (OGTT). Two kindreds (SA and LZ) were studied and compared to non-diabetic unrelated individuals (control group 1) matched for age and body mass index (BMI). In one kindred, some of these subjects were also obese (BMI >26 kg/m2), and other family members...

Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock

Palavras-chave: Proinsulin; Insulin; MODY; Glucokinase; Obesity.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400008

Camarini,R.; Benedito,M.A.C..

An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem...