|
|
|
| Registros recuperados: 601 | |
|
| |
|
|
| K. Awaneesh Singh; Patrick Celie; G. R. K. Rao; M. V. Jagannatham. |
| Formation and differentiation of salt crystal is a most common as well as challenging problem during protein crystallography. Although there are some methods used for their differentiation i.e. physical manipulation, birefringence, dye absorption and run a gel but all of them are totally depends on own perception and expertise as well as also require a good crystal (final stage crystal). To escape this problem the screening was done with PACT, JCSG and other automated screens as everyone do. But during optimization the additives/salts were ignored at first and optimization screen was made just based on different buffers and precipitant. After selecting suitable buffer, the type and percentage of precipitant was optimized. We got some ugly crystals and with... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Chemistry. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4725/version/1 |
| |
|
|
| I. C. Baianu; Valentin Prisecaru. |
| Amino acid NIR calibrations were developed in our Physical Chemistry of Foods Laboratory of the University of Illinois at Urbana for three selected amino acid groups that include essential amino acids for identified soybean accessions. Conventional “wet chemistry” analytical methods are time-consuming and costly. As a result, soybean breeders and researchers have an imperative need to utilize faster and less expensive methods. NIR Spectroscopy is a rapid and inexpensive method for composition analysis for academia and industry. Recent advancements in instrumentation design, such as the application of the Diode Array (DA) technique and the Fourier Transform (FT) IR and NIR techniques, have significantly improved overall instrument... |
| Tipo: Presentation |
Palavras-chave: Biotechnology; Chemistry; Genetics & Genomics; Plant Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6231/version/1 |
| |
|
| |
|
| |
|
|
| Sierra Rayne; Kaya Forest; Ken J. Friesen. |
| Perfluorinated acids (PFAs) are contaminants detected worldwide in a range of abiotic and biotic environmental matrices. The two major classes of PFAs include the perfluorinated carboxylic acids (PFCAs) and perfluorinated sulfonic acids (PFSAs), both of which are considered persistent and potentially bioaccumulative. Current research and regulatory efforts are focussed on the straight-chain members of each PFA class and homologue group, primarily because these congeners are the major components of technical mixtures and are also available as pure standards. However, the numerous potential branched congeners in each PFA class represent a poorly understood family of environmental contaminants whose environmental and toxicological properties may be more... |
| Tipo: Poster |
Palavras-chave: Chemistry; Earth & Environment. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1956/version/1 |
| |
|
|
| William L. Marshall. |
| The experimental static dielectric constants (_[epsilon]_) of water formulated by Fernández, et al., (1997-International Association for the Properties of Water and Steam, IAPWS), –35 to 600^o^C; 0.1 to 1200 MPa, are discovered to be closely described throughout by a simple equation of density (_[rho]_), presented here, and agreeing with their reported calculated (extrapolated) values to 923^o^C. Eight limiting boundary conditions are observed. Values of _[epsilon]_ follow isothermal straight-line slopes of log (_[epsilon]_ – 1) vs log _[rho]_ down to densities of 0.25 g cm^-3^ water, even in dioxane-water solutions. At lower densities, all slopes curve asymptotically in approaching unity, while becoming unity throughout... |
| Tipo: Manuscript |
Palavras-chave: Chemistry; Earth & Environment. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2472/version/1 |
| |
|
|
| Cristian Arsene; André Henrion; Nina Diekmann; Jenny Manolopoulou; Martin Bidlingmaier. |
| Inter-assay variation of antibody based routine tests is hampering comparability of measurement results for growth hormone (GH) between different laboratories and decision making in clinical practice. Here it is demonstrated, that quantification of GH by isotope dilution mass spectrometry (IDMS) constitutes a way to precise and reliable results which can be referred to in evaluation of performance of commercial test kits. With the IDMS method developed, tryptic cleavage products YSFLQNPQTSLCFSESIPTPSNR (T6) and LEDGSPR (T12) of GH are quantified by LC/MS-MS using the isotopically labeled forms of the peptides as internal standards. The GH cleavage fragments are obtained by whole-serum tryptic proteolysis and then extracted from the resulting mixture by... |
| Tipo: Manuscript |
Palavras-chave: Chemistry. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/4050/version/1 |
| |
|
| |
|
|
| Carsten C. Mahrenholz; Victor Tapia; Rolf Stigler; Rudolf Volkmer. |
| There are several methods commonly used to measure protein-protein interactions and binding affinities. Quite contrary to most of these methods, protein- and peptide arrays on cellulose membranes or glass slides are suitable for high-throughput measurement, as they provide a higher density of probes and a multitude of peptide-protein interactions can be measured in parallel [1]. The most important application of the SPOT synthesis technique is to simultaneously detect a high number of peptides that have a strong binding affinity to defined targets. The validity of the results, however, depends on the ability of the detection system to indicate binding events whilst not interfering with the experiment itself through cross reaction. We tested three common... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Chemistry. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4448/version/1 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Xuri Wu; Nan Liu; Yunmian He; Yijun Chen. |
| Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Microbiology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/1697/version/2 |
| |
|
|
| Sierra Rayne; Kaya Forest. |
| Aroma compounds in the Flavornet database were screened for ionizable functional groups such as carboxylic acids, aliphatic and aromatic amines, phenols, alcohols, and thiols. Of the 738 aroma compounds listed in this database, 101 molecules have ionizable moieties with estimated monomeric aqueous pK~a~ values ranging between 1.75 and 10.97. pH dependent effective air/water partitioning coefficients (K~aw,eff~) and n-octanol/water partitioning coefficients (D~ow~) were estimated for all ionizable aroma compounds over the pH range from 0 to 14. The ionizable aroma compounds display a broad range of K~aw,eff~ (1.8×10^-23^ to 6.1 atm M^-1^) and log D~ow~ (-6.2 to +7.2 units) values. For many aroma compounds, pH dependent ionization will have a... |
| Tipo: Manuscript |
Palavras-chave: Chemistry. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6269/version/1 |
| |
|
|
| Bernd Kastenholz. |
| Currently, no pharmaceuticals for the etiological treatment of degenerative protein-misfolding diseases (e.g., ALS, Alzheimer’s or prion diseases) are commercially available. Therefore, in this technical note theoretical considerations and practical approaches concerning the development of chaperone-based medications from medicinal plants (e.g., Ginkgo biloba) are reviewed and discussed in detail. Phytochaperones and other agents isolated from medicinal plants are proposed to serve as the general basis of drug development in protein-misfolding diseases. |
| Tipo: Manuscript |
Palavras-chave: Chemistry; Pharmacology; Plant Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1431/version/2 |
| |
|
|
| Sierra Rayne; Kaya Forest. |
| Gas phase standard state (298.15 K, 1 atm) geometries of the perchlorinated and perbrominated C~3~ through C~6~ n-alkanes were calculated using the B3LYP, B97D, M062X, and PBE0 density functionals with the 6-311++G(d,p) basis set. For both halogen classes and at all levels of theory, the C~3~ compounds adopt a trans conformation analogous to that of perfluoropropane. Similar to their perfluorinated counterparts, the perchlorinated and perbrominated C~4~ through C~6~ n-alkanes are predicted to have helical geometries. In contrast to perfluoroalkyl chains, the central CCCC dihedral angles of the perchlorinated and perbrominated derivatives are expected to decline with increasing chain length. |
| Tipo: Manuscript |
Palavras-chave: Chemistry. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5157/version/1 |
| |
|
| |
|
|
| Alaaeldin Salih; Erich A. Müller. |
| This work employs molecular modeling to simulate both the equilibrium and kinetic behavior pertaining adsorption/desorption and permeation of carbon dioxide within the enhanced coal bed methane (ECBM) process and the corresponding CO~2~ sequestration processes in deep coal mines. The coal structure is modeled on the basis of a carbon, nanotubes, slit pores and more realistic “virtual” carbons. (from bottom up in the figure) The grand canonical Monte Carlo method is used to simulate the adsorption isotherms and isosteric heats of adsorption of the methane-carbon dioxide-water ternary mixture into the carbon structure, while Molecular Dynamics is applied to the same systems to study the competitive diffusion properties of these fluids... |
| Tipo: Presentation |
Palavras-chave: Chemistry; Earth & Environment. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2625/version/1 |
| |
| Registros recuperados: 601 | |
|
|
|
