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| Registros recuperados: 63 | |
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| Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle. |
| The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae. |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf |
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| Dribek, Mohamed; Rinnert, Emmanuel; Colas, Florent; Crassous, Marie-pierre; Compere, Chantal. |
| We demonstrate the possible use of organometallic nanoprobe on competitive surface-enhanced Raman scattering immunoassay for policyclic aromatic hydrocarbon detection in sea water. This type of SERS immunoassay is based on to capture Raman reporter-labeled immuno-Au onto the immobilized layer of antigens (benzo[a]pyrene) on the gold substrate after incubation of these nanoprobes with PAHs including in the sample. To realize nanoprobes, first we have prepared gold nanoparticles (GNPs) according to Frens’ method. Then, we have labeled GNPs by the Raman reporter of 5,5’-Dithiobis(succinimidyl-2-nitrobenzoate) (DSNB). Thereafter, Benzo[a]pyrene antibody was attached to the DSNB labeled GNPs. Before use in SERS detection, their activity was controlled by... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19912/17576.pdf |
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| Gas, Fabienne; Baus, Beatrice; Pinto, Laetitia; Compere, Chantal; Tanchou, Valerie; Quemeneur, Eric. |
| Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Current methods for analyzing environmental samples are tedious and time consuming because they require taxonomists and animal experiments. New rapid detection methods, such as immunoassays, are sought for alerting purposes and for the study of algal ecodynamics in their natural environment. Alexandrium minutum, which causes paralytic shellfish poisoning, occurs with increasing frequency along European coasts. We have developed a one step immunochromatographic assay which is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies directed against surface antigens of A. minutum. The primary... |
| Tipo: Text |
Palavras-chave: Antibody; Alexandrium minutum; Immunochromatographic assay (ICA); Rapid detection. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00000/11153/7819.pdf |
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| Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert. |
| In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes. |
| Tipo: Text |
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf |
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| Carteau, David; Vallee-rehel, Karine; Linossier, Isabelle; Quiniou, Francoise; Davy, Romain; Compere, Chantal; Delbury, Maxime; Fay, Fabienne. |
| The development of new antifouling coatings with respect to the marine environment is actually crucial. The aim of the present work is to concept an erodible paint formulated with biodegradable polyester as binders and which combines two modes of prevention: chemical and physical repelling of biofouling. This system is principally dedicated to disturb durable settlement of microfouling. Each component was chosen according to its specific properties: chlorhexidine is a bisdiguanide antiseptic with antibacterial activity, zinc peroxide is an inorganic precursor of high instable entities which react with seawater to create hydrogen peroxide, Tween 85 is a non ionic surfactant disturbing interactions between colonizing organisms and surface. Obtained results... |
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Palavras-chave: Environmental coatings; Antifouling; Active substances; Microfouling. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00168/27923/27273.pdf |
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| Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal. |
| In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf |
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| Compere, Chantal; Bellon Fontaine, M.-n.; Bertrand, P; Costa, D; Marcus, P; Poleunis, C; Pradier, C.m.; Rondot, B; Walls, M.g.. |
| Adhesion of micro-organisms to surfaces in marine environments leads to biofouling. The deleterious effects of biofilm growth in the marine environment are numerous and include : energy losses due to increased fluid frictional resistance or to increased heat transfer resistance, risk of corrosion induced by micro-organisms, loss of optical properties, quality control and safety problems. Antifouling agents are generally used to protect surfaces from being affected by such a biofilm. These agents are toxic and can be persistent, causing harmful environmental and ecological effects. Moreover, the use of biocides and regular cleaning considerably increase the maintenance costs of marine industries. An improved knowledge of the biofilm adhesion mechanisms is... |
| Tipo: Text |
Palavras-chave: Biofilm; Surface characterisation; Carbohydrates; Protein; Seawater; Conditioning layer. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-1701.pdf |
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| Bustabad, E. A.; Garcia, G; Rodriguez-pardo, L.; Farina, J.; Perrot, H.; Gabrielli, C.; Bucur, B.; Lazerges, M.; Rose, D.; Compere, Chantal; Arnau, A.. |
| This work deals with the development of a high sensibility DNA biosensor based on a 50MHz Quartz Crystal Microbalance (QCM) Oscillator Electronic Circuit. The designed QCM oscillator sensor is able to detect the presence of complementary DNAs in a solution that match the sequence on a given strand in function of the changes in the output frequency of the oscillator. The design is adapted so that the Barkhausen condition is satisfied even when the quartz is immerged in liquid media. Also a comparative study of the developed 50MHz biosensor in front of a QCM oscillator with smaller frequency is carried out, with object of checking if the sensibility of the oscillator increases, allowing to detect smaller concentrations of the complementary DNA. |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00021/13198/10574.pdf |
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| Salaun, Stephanie; Rinnert, Emmanuel; Compere, Chantal. |
| Cette étude s’inscrit dans le cadre du projet REI n°2010 34 0010 intitulé « Biofilms et corrosion d’alliages inoxydables en eau de mer », et a pour objectif de caractériser le biofilm responsable de l’activation de la corrosion des alliages inoxydables en eau de mer par une approche pluridisciplinaire, dans le but d’identifier les composantes responsables de ce type de corrosion. Ce biofilm spécifique est ainsi en cours de caractérisation par des méthodes de microscopie Apotome et de spectroscopie Raman. Ce poster a fait l'objet d'une présentation lors de l'Ecole thématique du CNRS : Voyage au centre d'un biofilm : physico-chimie des exopolymères de l'espace inter-cellulaire. Nancy, les 27 et 29 septembre 2011. |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00057/16775/14228.pdf |
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| Peron, Olivier; Rinnert, Emmanuel; Toury, T.; De La Chapelle, M. Lamy; Compere, Chantal. |
| In the investigation of chemical pollutants, such as PAHs (Polycyclic Aromatic Hydrocarbons) at low concentration in aqueous medium, Surface-Enhanced Raman Scattering (SERS) stands for an alternative to the inherent low cross-section of normal Raman scattering. Indeed, SERS is a very sensitive spectroscopic technique due to the excitation of the surface plasmon modes of the nanostructured metallic film. The surface of quartz substrates was coated with a hydrophobic film obtained by silanization and subsequently reacted with polystyrene (PS) beads coated with gold nanoparticles. The hydrophobic surface of the SERS substrates pre-concentrates non-polar molecules such as naphthalene. Under laser excitation, the SERS-active substrates allow the detection and... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00026/13760/10890.pdf |
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| Bellon-fontaine, M.-n.; Bertrand, P; Compere, Chantal; Costa, D; Guezennec, Jean; Marcus, P; Poleunis, C.; Pradier, C.-m.; Rondot, B; Walls, M.g.. |
| Numerous works are reported in literature concerning the biofilm growth on surfaces in seawater but the understanding of the adhesion mechanisms in the first steps of immersion is still subject to investigations. A knowledge of the chemical composition and of the kinetics of conditioning layer formation is a central requirement for a sound scientific understanding of biofilm growth. Due to its complexity and to the interdisciplinary nature of the subject, this research asks for the collaboration between the fields of material science, surface analyses, chemistry, marine microbiology and biochemistry. The first steps of biofilm formation on 316L stainless steels immersed in natural sea water have been studied, using a broad range of surface characterisation... |
| Tipo: Text |
Palavras-chave: Sea water; Substrates; Biofilm. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-1699.pdf |
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| Uher, Emmanuelle; Compere, Chantal; Combe, Matthieu; Mazeas, Florence; Gourlay-france, Catherine. |
| Concerning in situ passive sampler deployment, several technical priorities must be considered. In particular, deployment time must be sufficiently long not only to allow a significant quantity to be accumulated to facilitate analysis but also to ensure that the signal is above the quantification limit and out of the blank influence. Moreover, regarding the diffusive gradient in thin films (DGT) technique, deployment time must also be sufficiently long (at least 5 days) to avoid the interactions of the solutes with the material diffusion layer of the DGT and for the steady state to be reached in the gel. However, biofouling occurs in situ and modifies the surface of the samplers. In this article, we propose a kinetic model which highlights the biofouling... |
| Tipo: Text |
Palavras-chave: DGT; Metals; Biofouling; Seine River; Field deployment; Passive sampler. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00382/49330/49759.pdf |
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| Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal. |
| We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA... |
| Tipo: Text |
Palavras-chave: QCM; DNA; Biosensor. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf |
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| Registros recuperados: 63 | |
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