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| Registros recuperados: 67 | |
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| Pommepuy, Monique. |
| Les estuaires, zones de mélange d'eaux douces et d'eaux marines, ont, de tout temps, été l'objet de multiples activités, tant industrielles que maricoles (élevage de coquillages, pêche,... ) L'agglomération autour d'une zone relativement fermée, d'une population humaine et de ses activités, a des conséquences obligatoires sur la faune et sur la flore de l'estuaire et, en particulier, sur les populations bactériennes. En effet, le rejet d'eaux usées, fortement chargées en bactéries contaminantes et en matière organique, dans une zone où le renouvellement des eaux se fait de façon relativement lente, favorise la survie des bactéries étrangères au milieu. De même, les apports en éléments nutritifs modifient la flore marine autochtone. Enfin, la présence de... |
| Tipo: Text |
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| Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/00088/19965/17632.pdf |
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| Coutard, Francois; Pommepuy, Monique; Loaec, Solen; Hervio Heath, Dominique. |
| Aims: This work investigates the maintenance of viability and potential virulence of Vibrio parahaemolyticus in a viable but nonculturable population (VBNC) state by reverse transcription-polymerase chain reaction (RT-PCR). Methods and Results: Housekeeping genes, 16S-23S rDNA and rpoS, as well as virulence genes, tdh1 and tdh2, were selected and detected by PCR in a pathogenic strain of V. parahaemolyticus (Vp4). Their expression was then studied by RT-PCR in V. parahaemolyticus Vp4 cultivated in rich medium at 37degreesC. The 16S-23S rDNA and rpoS, tdh1, tdh2 genes were transcripted at the mid-logarithmic, stationary and late stationary phases, corresponding to various physiological states. The expression of these genes was also studied by RT-PCR in a... |
| Tipo: Text |
Palavras-chave: Vibrio parahaemolyticus; Viable but nonculturable state; Tdh2; Tdh1; RT PCR; RpoS; Environment; 16S 23S rDNA. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1098.pdf |
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| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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| Monfort, Patrick; Hervio Heath, Dominique; Caprais, Marie-paule; Pommepuy, Monique; Annezo, Jean-pierre; Loaec, Solen; Le Mennec, Cecile; Guillerm, Emmanuelle; Boulben, Sylviane; Bilien, Gwenael; Bonsor, Robert; Porter, Jonathan; Pickup, Roger. |
| Le projet européen Cycleau, initié à l'échelle locale sur la problèmatique de l'envasement et de l'ensablement de l'estuaire du Bélon, y a également intégré un volet bactériologique en raison des contraintes fortes que faisaient peser les pics conjoncturels de contamination sur la profession conchylicole (fermetures temporaires, déclassement éventuel) mais plus largement sur l'image du territoire (impact touristique) et en définitive sur son développement durable. Pour satisfaire la restauration de la qualité des eaux estuariennes, l'IFREMER a fait appel à un certain nombre d'outils susceptibles de contribuer à la formulation d'un diagnostic pertinent et ainsi de répondre aux préoccupations des acteurs du littoral. Pour ce faire, nous avons créé un réseau... |
| Tipo: Text |
Palavras-chave: Bassin versant; Contamination fécale; Escherichia coli; Flux bactériens; Bactériophages; SIG; Évaluation. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00200/31077/29490.pdf |
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| Guillois-becel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A. M.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, V.. |
| Following the notification of nine hepatitis A cases clustered in the Cotes d'Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d'Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were... |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6685.pdf |
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| Da Silva, A; Le Saux, Jean-claude; Parnaudeau, Sylvain; Pommepuy, Monique; Elimelech, M; Le Guyader, Soizick. |
| Noroviruses, an important cause of gastroenteritis, are excreted by infected individuals and are therefore present in wastewater. We quantified norovirus genogroup I (GI) and GII in wastewater at different locations in France and evaluated removal by a range of treatment types, including basic (waste stabilization pond), current industry standard (activated sludge), and state-of-the-art (submerged membrane bioreactor) treatments. Noroviruses were quantified using real-time reverse transcription-PCR (rRT-PCR). Mengovirus was used as a virus extraction control, and internal controls were used to verify the level of GI and GII rRT-PCR inhibition. A total of 161 (81 influent and 79 effluent) samples were examined; GI and GII were detected in 43 and 88% of the... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3571.pdf |
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| Pommepuy, Monique; Caprais, Marie-paule; Le Saux, Jean-claude; Le Mennec, Cecile; Parnaudeau, Sylvain; Madec, Yvon; Monier, Martial; Brest, Goulven; Le Guyader, Francoise. |
| Depuration processes try to eliminate microorganisms using seawater to allow living, filter-feeding shellfish to naturally purge themselves from agents they accumulated from the environment. Until now the only parameter to evaluate depuration was the decrease in E. coli counts. Studies have shown that viruses can persist in the environment longer than E. coli and that shellfish meeting the endstandard (< 230 E.colil /100g) have been involved in viral outbreaks. Starting in February 2000, the European Commission has been funding (Fifth Framework Programme), a 3-year project entitled "Virus Safe Seafood". One of the specific objectives of this project is to innovate technology for shellfish depuration. For this purpose a depuration system was designed and... |
| Tipo: Text |
Palavras-chave: Bacteriophage FRNA; Depuration; Shellfish. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-1208.pdf |
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| Guillois-bécel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A.m.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, C.. |
| Following the notification of nine hepatitis A cases partly clustered near an oyster growing area in the Côtes d’Armor district (Brittany, France), epidemiological, environmental and microbiological investigations were performed in order to identify the source and vehicle of contamination and devise control measures. The investigations identified 111 cases, all of whom lived or had stayed in the district. Of these cases, 87% had eaten raw bivalve molluscs and 81% had specifically eaten oysters. The raw molluscs originated from a single shellfish farm located at the north of Paimpol bay. The shellfish were probably contaminated while being stored in submersible tanks or purified in a land-based tank. They were tainted either by sewage overflows or releases... |
| Tipo: Text |
Palavras-chave: Hepatitis A; Outbreak; Oysters; Epidemiological investigations; Traceback investigations. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17750/15272.pdf |
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| Pommepuy, Monique; Cormier, Michel; Brunel, Laurence; Breton, Marguerite. |
| The purpose of this study is to evaluate the behaviour of the bacterial flora of a macrotidal estuary, originating from different sources: the Elorn freshwater river, a sewage treatment plant (STP), and the sediment. Viable counts were done using severa} culture media in order to determine where bacteria originated. Principal component analysis showed that the estuary was subjected to two well-defined conditions. On the one band, during periods of low turbulence (neap tide), resuspension of bottom sediments did not occur. Bacterial flora decreased from the upper reaches of the estuary to its lower part, coming mainly from two sources (river and STP). As a function of salinity variations, bacterial flora showed a progressive increase of the endogeneous... |
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| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00109/22069/19711.pdf |
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| Pommepuy, Monique. |
| Conformément aux termes d'un contrat passé entre le CNEXO et le Service Départemental de l’Équipement, l’Unité Littoral Centre Océanologique de Bretagne a effectué de janvier à septembre 1977, plusieurs campagnes océanographiques en rade de BREST. Les objectifs pouIsuivis etaient 1 - d'estimer la dispersion des eaux fluviales dans la rade pendant des marées de vives-eaux et de mortes-eaux et durant des périodes de crue et d'étiage des rivières 2 - de suivre, sur la tranche d'eau, en plusieurs points de la rade et au cours d'un cycle complet de marée, l'évolution des paramètres suivants: courant, température, salinité, sels nutritifs, matières en suspension; 3 - de tracer les trajectoires probables d'un rejet hypothétique en un point donné et à une heure... |
| Tipo: Text |
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| Ano: 1977 |
URL: http://archimer.ifremer.fr/doc/00055/16578/14080.pdf |
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| Maalouf, Haifa; Pommepuy, Monique; Le Guyader, Soizick. |
| Human fecal wastes contain a large variety of viruses that can enter the environment through discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. To explain bivalve mollusks contamination, several factors including human epidemiology, virus persistence through sewage treatment plant, and shellfish uptake may be suggested. Considering different outbreaks described in the literature, the most common route for transmission is accidental contamination after heavy rainfall, when extra loads cause an overflow, and release of untreated sewage into the... |
| Tipo: Text |
Palavras-chave: Shellfish; Outbreaks; Norovirus; Environmental conditions. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12252/9031.pdf |
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| Registros recuperados: 67 | |
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