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| Registros recuperados: 54 | |
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| Sinha,Rupesh Kumar; Krishnan,Kottekkatu Padinchati; Hatha,Ammanamveetil Abdulla Mohamed; Rahiman,Mujeeb; Thresyamma,Divya David; Kerkar,Savita. |
| Abstract The diversity and abundance of retrievable pelagic heterotrophic bacteria in Kongsfjorden, an Arctic fjord, was studied during the summer of 2011 (June, August, and September). Retrievable bacterial load ranged from 103 to 107 CFU L−1 in June, while it was 104-106 CFU L−1 in August and September. Based on 16S rRNA gene sequence similarities, a higher number of phylotypes was observed during August (22 phylotypes) compared to that during June (6 phylotypes) and September (12 phylotypes). The groups were classified into four phyla: Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Bacteroidetes was represented only by a single member Leewenhoekiella aequorea during the three months and was dominant (40%) in June. However, this dominance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kongsfjorden; Retrievable heterotrophic bacteria; 16S rRNA; Arctic fjord. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100051 |
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| Pillai,Aneesh Balakrishna; Kumar,Arjun Jaya; Thulasi,Kavitha; Kumarapillai,Harikrishnan. |
| Abstract This study was focused on the polyhydroxybutyrate (PHB) accumulation property of Bacillus aryabhattai isolated from environment. Twenty-four polyhydroxyalkanoate (PHA) producers were screened out from sixty-two environmental bacterial isolates based on Sudan Black B colony staining. Based on their PHA accumulation property, six promising isolates were further screened out. The most productive isolate PHB10 was identified as B. aryabhattai PHB10. The polymer production maxima were 3.264 g/L, 2.181 g/L, 1.47 g/L, 1.742 g/L and 1.786 g/L in glucose, fructose, maltose, starch and glycerol respectively. The bacterial culture reached its stationary and declining phases at 18 h and 21 h respectively and indicated growth-associated PHB production. Nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus aryabhattai PHB10; Polyhydroxybutyrate; Domestic sewerage; Polymer characterization; 16S rRNA. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300451 |
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| De La Torre-Ruiz,Neyser; Ruiz-Valdiviezo,Víctor Manuel; Rincón-Molina,Clara Ivette; Rodríguez-Mendiola,Martha; Arias-Castro,Carlos; Gutiérrez-Miceli,Federico Antonio; Palomeque-Dominguez,Héctor; Rincón-Rosales,Reiner. |
| ABSTRACT The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agave; Inoculation; Bacteria; Inulin; 16S rRNA. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300587 |
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| Ramya,Shanivarsanthe Leelesh; Venkatesan,Thiruvengadam; Srinivasa Murthy,Kottilingam; Jalali,Sushil Kumar; Verghese,Abraham. |
| Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plutella xylostella; Bacteria; 16S rRNA; Phylogeny; Carboxylesterase; Indoxacarb. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200327 |
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| Huang,Haibin; Unver,Ahmet; Perez,Miriam J.; Orellana,Nelson G.; Rikihisa,Yasuko. |
| Blood specimens from clinically normal military dogs and their trainers, in Lara, Venezuela were screened for Anaplasma platys, A. phagocytophilum, or Ehrlichia ewingii using 16S rRNA PCR tests. Sixteen percent (7/43) of dog specimens were positive by A. platys PCR test followed by sequencing of the PCR products, and all human blood specimens [25] were negative. All specimens from these dogs and humans were PCR negative for E. ewingii or A. phagocytophilum. Twelve Rhipicephalus sanguineus ticks removed from these dogs were negative for A. platys by reverse transcription PCR test. Almost the entire 16S rRNA gene (1,364 bp) and groESL operon (1,646 bp) sequences of A. platys isolated from a dog were determined, revealing that both sequences were closely... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 16S rRNA; GroESL operon; Anaplasma platys; Dogs; Venezuela. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300002 |
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| Dharne,M.S.; Gupta,A.K.; Rangrez,A.Y.; Ghate,H.V.; Patole,M.S.; Shouche,Y.S.. |
| Flesh flies (Diptera: Sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. Antimicrobial studies of Myroides spp. are restricted to nosocomial strains. A Gram-negative bacterium, Myroides sp., was isolated from the gut of adult flesh flies (Sarcophaga sp.) and submitted to evaluation of nutritional parameters using Biolog GN, 16S rRNA gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of metallo β-lactamase genes (TUS/MUS). The antagonistic effects were tested on Gram-negative and Gram-positive bacteria isolated from human clinical specimens, environmental samples and insect mid gut. Bacterial species included were Aeromonas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flesh fly; Myroides sp.; 16S rRNA; Antibacterial activity; Metallo beta-lactamase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200035 |
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| Rodrigues,Dalila Ribeiro; Silva,Aleksandro Ferreira da; Cavalcanti,Maria Idaline Pessoa; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Ribeiro,Paula Rose de Almeida; Ferreira Neto,Reginaldo Alves; Freitas,Ana Dolores Santiago de; Fernandes-Júnior,Paulo Ivan. |
| Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological nitrogen fixation; "Mulungu"; Diversity; Inoculant; 16S rRNA. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300503 |
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| AlMogbel,Mohammed Suliman. |
| Abstract The aim of this study was to identify different Clostridium spp. isolated from currency notes from the Ha’il region of Saudi Arabia in September 2014 using MALDI–TOF-MS. Clostridium spp. were identified by Bruker MALDI–TOF-MS and compared with VITEK 2. The confirmation of the presence of different Clostridium spp. was performed by determining the sequence of the 16S ribosomal RNA gene. In this study, 144 Clostridium spp. were isolated. Among these specimens, MALDI–TOF-MS could identify 88.8% (128/144) of the isolates to the species level and 92.3% (133/144) to the genus level, whereas, VITEK 2 identified 77.7% of the (112/144) isolates. The correct identification of the 144 isolates was performed by sequence analysis of the 500 bp 16S rRNA gene.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium species; MALDI–TOF-MS; VITEK 2; 16S rRNA. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200410 |
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| El-Naggar,Noura El-Ahmady; Abdelwahed,Nayera A.M.; Saber,Wesam I.A.; Mohamed,Asem A.. |
| The use of low cost agro-industrial residues for the production of industrial enzymes is one of the ways to reduce significantly production costs. Cellulase producing actinomycetes were isolated from soil and decayed agricultural wastes. Among them, a potential culture, strain NEAE-J, was selected and identified on the basis of morphological, cultural, physiological and chemotaxonomic properties, together with 16S rDNA sequence. It is proposed that strain NEAE-J should be included in the species Streptomyces albogriseolus as a representative of a novel sub-species, Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J and sequencing product was deposited in the GenBank database under accession number JN229412. This organism was tested for its... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agro-industrial residues; Endoglucanase production; Streptomyces albogriseolus; 16S rRNA; Scanning electron microscope. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200050 |
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| Registros recuperados: 54 | |
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