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Registros recuperados: 53
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Genomics and X-ray microanalysis indicate that Ca2+ and thiols mediate the aggregation and adhesion of Xylella fastidiosa BJMBR
Leite,B.; Ishida,M.L.; Alves,E.; Carrer,H.; Pascholati,S.F.; Kitajima,E.W..
The availability of the genome sequence of the bacterial plant pathogen Xylella fastidiosa, the causal agent of citrus variegated chlorosis, is accelerating important investigations concerning its pathogenicity. Plant vessel occlusion is critical for symptom development. The objective of the present study was to search for information that would help to explain the adhesion of X. fastidiosa cells to the xylem. Scanning electron microscopy revealed that adhesion may occur without the fastidium gum, an exopolysaccharide produced by X. fastidiosa, and X-ray microanalysis demonstrated the presence of elemental sulfur both in cells grown in vitro and in cells found inside plant vessels, indicating that the sulfur signal is generated by the pathogen surface....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Xylella fastidiosa; Aggregation; Adhesion; Methionine sulfoxide reductase; Biofilm.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600003
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Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation BJMBR
Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V..
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007
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Leukocyte adhesion - a fundamental process in leukocyte physiology BJMBR
Gahmberg,C.G.; Valmu,L.; Tian,L.; Kotovuori,P.; Fagerholm,S.; Kotovuori,A.; Kantor,C.; Hilden,T..
Leukocyte adhesion is of pivotal functional importance. The adhesion involves several different adhesion molecules, the most important of which are the leukocyte ß2-integrins (CD11/CD18), the intercellular adhesion molecules, and the selectins. We and others have extensively studied the specificity and binding sites in the integrins and the intercellular adhesion molecules for their receptors and ligands. The integrins have to become activated to exert their functions but the possible mechanisms of activation remain poorly understood. Importantly, a few novel intercellular adhesion molecules have been recently described, which seem to function only in specific tissues. Furthermore, it is becoming increasingly apparent that changes in integrins and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocyte; Adhesion; Integrin; ICAM; Membrane; Glycoprotein.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500003
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Growth kinetics of Escherichia coli O157:H7 on the epicarp of fresh vegetables and fruits BJM
Gullian-Klanian,Mariel; Sánchez-Solis,Maria José.
ABSTRACT Despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of Escherichia coli O157:H7 on different plant substrates. This study analyzed the selective adhesion and growth of E. coli O157:H7 on chili habanero (Capsicum chinense L.), cucumber (Cucumis sativus), radish (Raphanus sativus), tomato (Lycopersicon esculentum), beet (Beta vulgaris subsp. vulgaris), and onion (Allium cepa L.) under laboratory conditions. The Gompertz parameters were used to determine the growth kinetics. Scanning electron microscopy was used to visualize the adhesion of E. coli O157:H7 on the epicarp of the samples. Predictive models were constructed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: E. coli O157:H7; Adhesion; Predictive growth; Low selectivity; Fruit and vegetables.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100104
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Effects of growth temperature on polystyrene adhesion of Pseudomonas aeruginosa ATCC 27853 BJM
Cappello,Simone; Guglielmino,Salvatore P.P..
The importance of avoiding bacterial adhesion to plastic medical devices is recognized. To better understand the interaction between bacteria and surface, we studied the influence of different growth temperatures (15, 30 and 47ºC) on the polystyrene adhesion capacity of Pseudomonas aeruginosa ATCC 27853.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pseudomonas aeruginosa; Temperature; Hydrophobicity; Adhesion.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300001
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Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia culture and protection against pathogens BJM
Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017
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Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface BJM
Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036
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Adhesion of Salmonella Enteritidis to stainless steel surfaces BJM
Oliveira,Kelly; Oliveira,Tereza; Teixeira,Pilar; Azeredo,Joana; Oliveira,Rosário.
Adhesion of microorganisms to food processing surfaces and the problems it causes are a matter of strong concern to the food industry. Contaminated food processing surfaces may act as potential sources of transmission of pathogens in food industry, catering and in the domestic environments. Several studies have shown that adhesion of bacteria to surfaces partly depends upon the nature of the inert surfaces and partly upon the bacterial surface properties. The aim of this study was to compare the adhesion of four different strains of Salmonella Enteritidis to stainless steel 304 (SS 304). The effect of surface hydrophobicity and surface elemental composition on the adhesion process was also analysed. Hydrophobicity was evaluated through contact angle...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Salmonella Enteritidis; Hydrophobicity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200026
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Curli fimbria: an Escherichia coli adhesin associated with human cystitis BJM
Cordeiro,Melina Aparecida; Werle,Catierine Hirsch; Milanez,Guilherme Paier; Yano,Tomomasa.
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curli fimbria; Adhesion; Escherichia coli; UPEC.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200414
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Assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of Bacillus cereus from a dairy plant BJM
Bernardes,Patrícia Campos; Andrade,Nélio José de; Ferreira,Sukarno Olavo; Sá,João Paulo Natalino de; Araújo,Emiliane Andrade; Delatorre,Deyse Maria Zanom; Luiz,Lívia Maria Pinheiro.
The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically favorable (ΔGadhesion > 0) between the stainless steel and the strain of B. cereus studied. Thus, the interaction between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrophobicity; Roughness; Bacillus cereus; Dairy plant; Adhesion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400017
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Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene BJM
Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022
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Adhesion and invasion of Clostridium perfringens type A into epithelial cells BJM
Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J..
ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764
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Hyaluronic acid and synovial fluid in preventing adhesion formation after tenorrhaphy: An in vivo study on rabbit Achilles tendon Ciência Rural
Kurt,Başak; Ozaydin,Isa; Sozmen,Mahmut; Bilge,Ali; Cihan,Mete; Hamamci,Emre; Aydin,Ugur.
ABSTRACT: Peritendinous adhesions are a significant problem in tendon surgery. One of the most preferred methods for preventing adhesion formation is anti-adhesive applications. This study aimed to investigate the efficacy of natural synovial fluid as an alternative viscoelastic agent for preventing adhesion in the healing period compared to hyaluronic acid. Thirty adult New Zealand rabbits were used in the study to form three experimental models. In all groups, a rupture was induced in the Achilles tendons. Following tenorrhaphy, hyaluronic acid was applied in one group and synovial fluid in the other group for anti-adhesive purposes. In the control group, no viscoelastic application was made. Bandage was applied to the operated extremities for three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achilles tendon; Adhesion; Synovial fluid; Hyaluronic acid; Rabbit.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700601
Registros recuperados: 53
Primeira ... 123 ... Última
 

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