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Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14 BABT
Carvalho,André Lacerda Ulysses de; Oliveira,Fábio Henrique Portella Corrêa de; Mariano,Rosa de Lima Ramos; Gouveia,Ester Ribeiro; Souto-Maior,Ana Maria.
The physiology of B. subtilis R14 was investigated in minimal medium under excess-oxygen and oxygen-limited conditions. Growth and efficient sporulation could be achieved in excess-oxygen culture on medium with readily metabolizable carbon and nitrogen sources, which allowed high growth rate and high biomass yield. A short transition phase between the exponential growth and sporulation could be attained by formulating a medium with a well-balanced C/N ratio. Under oxygen-limitation, but in the presence of essential nutrients (i.e. excess-nutrient cultivation), B. subtilis R14 produced bioactive compounds, which showed activity against several phytopathogenic bacteria. Under anaerobic condition, the organism did not grow neither through fermentation nor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Batch cultivation; Oxygen availability; Antimicrobial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300020
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Control of papaya fruits anthracnose by essential oil of Ricinus communis BABT
Siqueira Júnior,César Luis; Freire,Maria das Graças Machado; Moreira,Antônio Sérgio Nascimento; Macedo,Maria Ligia Rodrigues.
The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v) were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v) concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v) castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Antimicrobial activity; Essential oil; Postharvest; Colletotrichum gloeosporioides; Papaya.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100009
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Antimicrobial activity of essential oils from Pimenta pseudocaryophyllus and Tynanthus micranthus BABT
Custódio,Dayana Lacerda; Burgo,Rafaela Pinheiro; Moriel,Bárbara; Barbosa,Aneli de Melo; Rezende,Maria Ines; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Bianchini,Edmilson; Faria,Terezinha de Jesus.
The present study describes the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from the leaves of Pimenta pseudocaryophyllus (1.0% w/w) and Tynanthus micranthus (1.1% w/w). GC and GC/MS analysis demonstrated that eugenol was the only component in the T. micranthus essential oil (99.9%) and the major component in the P. pseudocaryophyllus essential oil (92.59%), which also presented methyleugenol, terpinen-4-ol, o-cymene and (E)-caryophyllene, among others. Both the oils presented antimicrobial activity against bacteria, yeast and filamentous fungi tested.The Bioautography test revealed that eugenol was the bioactive component in both the oils against Cladosporium herbarum. This is the first report about the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pimenta pseudocaryophyllus; Tynanthus micranthus; Essential oil; Antimicrobial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600013
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GC-MS analysis of Nigella sativa seeds and antimicrobial activity of its volatile oil BABT
Gerige,Saptha Jyothi; Gerige,Mahesh Kumar Yadav; Rao,Muralidhara; Ramanjaneyulu,.
The present study dealt with the hydro distillation of Nigella sativa seeds and GC-MS analysis. The total composition of the oil was 86.7%. The seed volatile oil was tested against 19 microbes (Gram positive, Gram negative and fungi), which showed that Gram negative bacteria Haemophilus influenza, Klebsiella pnemoniae, and Proteus vulgaris were highly sensitive against the antimicrobial agent, whereas the fungi such as Trichoderma vibriae,. Pencillium rubrum; and Dermatophyte: Trichophyton mentagrophytes; had no response at the 20µl concentration.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nigella sativa; Volatile oil; Antimicrobial activity; Antifungal activity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500016
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Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces BABT
Ferreira,Alessandra Einsfeld; Canal,Natália; Morales,Daiana; Fuentefria,Daiane Bopp; Corção,Gertrudes.
The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus mundtii; Enterocins; Antimicrobial activity; Biopreservative.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200010
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Elicitation Enhanced the Production of Phenolic Compounds and Biological Activities in Hairy Root Cultures of Bitter melon ( Momordica charantia L.) BABT
Chung,Ill-Min; Thiruvengadam,Muthu; Rekha,Kaliyaperumal; Rajakumar,Govindasamy.
ABSTRACT Momordica charantia (Cucurbitaceae) is an important vegetable and also medicinal crop which produces the bioactive compounds for various biological activities with potential uses in human health. The present investigation relates to elicitors of jasmonic acid (JA) and salicylic acid (SA) to enhance biomass accumulation and phenolic compound production in hairy root cultures of M. charantia. Hairy root cultures were elicited with JA and SA at 0, 25, 50 and 100 μM concentrations respectively. The adding of elicitation to the hairy root cultures on the 15th day of culture and the roots were harvested on day 25. Cultures supplemented with 100 μM JA and SA enhanced the phenolic compounds significantly compared to that of non-elicited hairy root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticancer activity; Antioxidant activity; Antimicrobial activity; Elicitation; Hairy root cultures; Phenolic compounds.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100430
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Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract BABT
Naine,S. Jemimah; Devi,C. Subathra; Mohanasrinivasan,V.; B,Vaishnavi.
The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marine actinomycetes; Streptomyces parvulusVITJS11; Antimicrobial activity; Antioxidants; Cytotoxicity; Bioactive compounds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200198
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Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens BABT
Mittal,Jitendra; Singh,Abhijeet; Batra,Amla; Sharma,Madan Mohan.
The present study was conducted to explore the hidden potential of natural products synthesized in the medicinal plant Tinosporacordifolia. This plantis prioritized by National Medicinal Plant Board, New Delhi. Leaf and inter nodal segments were inoculated on MS Medium fortified with IBA (1.0 mg/L) produced callus after four weeks. The calli were brown due to phenolic substance secreted by the explant. This problem was overcome by using adjuvant PVP (0.1%). Further, secondary metabolites were isolated from callus and field leaf through soxhlet extractor and fractionated by using column chromatography. The antibacterial activity of these fractioned extracts from Tinosporacordifolia callus and leaf were seen against multi drug resistance bacteria viz.,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Callus; Natural products; PVP; Antimicrobial activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100406
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Endophytic fungi occurring in Ipomoea carnea tissues and their antimicrobial potentials BABT
Tayung,Kumanand; Sarkar,Meenakshi; Baruah,Paran.
The aim of this work was to study the endophytic fungi associated with the tissues of Ipomoea carnea, a common invasive plant of India. A total of 69 isolates belonging to ten taxa comprising 1.45% Zygomycetes, 10.14% Coelomycetes, 62.32% Hypomycetes, 18.84% sterile mycelia and 7.25% unidentified species were obtained. Species of Curvularia, Aspergillus, Fusarium, Colletotrichum and sterile fungus were isolated as dominant endophytes. Colonization frequency of Curvularia (7.25%) was highest which was isolated from all the tissues. The samples collected during the monsoon harbored more endophytes and showed higher species richness than the samples obtained in summer season. Of the total isolates, 15 isolates (21.74%) displayed antimicrobial activity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea carnea; Endophytic fungi; Curvularia; Antimicrobial activity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500003
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Antimicrobial activity of Syagrus coronata (Martius) Beccari BABT
Hughes,Alice Ferreira da Silva; Lima,Fabíola Guedes de; Lucchese,Angélica Maria; Góes Neto,Aristóteles; Uetanabaro,Ana Paula Trovatti.
This work aimed to evaluate the antimicrobial activity of aqueous and methanol extracts of leaves, inflorescences, nut-shell, liquid and solid endosperm nuts of Syagrus coronata against pathogenic bacteria and yeast. Screening was initially performed using the agar dilution method. The extracts regarded as bioactive underwent liquid-liquid partition for determination of their minimum inhibitory concentration and minimum bactericide concentration (MIC and MBC) and those of their respective fractions against the microorganisms inhibited in preliminary tests. Antimicrobial activity was observed only in inflorescences. The corresponding aqueous extract was effective against B. cereus and the three strains of S. aureus, and the corresponding MIC and MCB values...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Syagrus coronata; Arecaceae; Antimicrobial activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200012
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Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey BABT
Gomaa,Eman Zakaria.
The aim of the present study was to investigate the antimicrobial effect of the lipopeptide biosurfactants produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using the disc-diffusion method against different Gram-positive bacteria {B subtilis, B. thuringiensis (two strains), B. cereus, Staphylococcus aureus (two strains) and Listeria monocytogenes)}, Gram-negative bacteria {(Pseudomonas aeruginosa, Escherichia coli (two strains), Salmonella typhimurium, Proteous vulgaris and Klebsiella pneumoniae) and a yeast (Candida albicans)}. The biosurfactant showed profoundly distinct antibacterial activity toward tested bacteria and displayed an antifungal activity against the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosurfactant; Bacillus licheniformis; Cheese whey; Antimicrobial activity; Staphylococcus aureus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200011
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Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees BJMBR
Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S..
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617
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Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens BJMBR
Botelho,M.A.; Nogueira,N.A.P.; Bastos,G.M.; Fonseca,S.G.C.; Lemos,T.L.G.; Matos,F.J.A.; Montenegro,D.; Heukelbach,J.; Rao,V.S.; Brito,G.A.C..
Dental caries and periodontal disease are associated with oral pathogens. Several plant derivatives have been evaluated with respect to their antimicrobial effects against such pathogenic microorganisms. Lippia sidoides Cham (Verbenaceae), popularly known as "Alecrim-pimenta" is a typical shrub commonly found in the Northeast of Brazil. Many plant species belonging to the genus Lippia yield very fragrant essential oils of potential economic value which are used by the industry for the commercial production of perfumes, creams, lotions, and deodorants. Since the leaves of L. sidoides are also extensively used in popular medicine for the treatment of skin wounds and cuts, the objective of the present study was to evaluate the composition and antimicrobial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lippia sidoides; Essential oil; Antimicrobial activity; Oral pathogens.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300010
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Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark BJMBR
Kumar,R.S.; Sivakumar,T.; Sunderam,R.S.; Gupta,M.; Mazumdar,U.K.; Gomathi,P.; Rajeshwar,Y.; Saravanan,S.; Kumar,M.S.; Murugesh,K.; Kumar,K.A..
The present study was carried out to evaluate the antioxidant and antimicrobial activities of a methanol extract of Bauhinia racemosa (MEBR) (Caesalpiniaceae) stem bark in various systems. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the methanol extract increased in a concentration-dependent manner. About 50, 100, 250, and 500 µg MEBR inhibited the peroxidation of a linoleic acid emulsion by 62.43, 67.21, 71.04, and 76.83%, respectively. Similarly, the effect of MEBR on reducing power increased in a concentration-dependent manner. In DPPH radical scavenging assays the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bauhinia racemosa; Antioxidant activity; Lipid peroxidation; Scavenging superoxide; DPPH assay; Antimicrobial activity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700004
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Antioxidant and antimicrobial activities of bitter and sweet apricot (Prunus armeniaca L.) kernels BJMBR
Yiğit,D.; Yiğit,N.; Mavi,A..
Abstract <img border=0 id="_x0000_i1034" src="../img/back.gif"> The present study describes the in vitro antimicrobial and antioxidant activity of methanol and water extracts of sweet and bitter apricot (Prunus armeniaca L.) kernels. The antioxidant properties of apricot kernels were evaluated by determining radical scavenging power, lipid peroxidation inhibition activity and total phenol content measured with a DPPH test, the thiocyanate method and the Folin method, respectively. In contrast to extracts of the bitter kernels, both the water and methanol extracts of sweet kernels have antioxidant potential. The highest percent inhibition of lipid peroxidation (69%) and total phenolic content (7.9 ± 0.2 µg/mL) were detected in the methanol extract of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apricot (Prunus armeniaca L.) kernels; Antioxidant activity; Antimicrobial activity; Total phenolic compounds.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400006
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Screening for antimicrobial activity of natural products using a microplate photometer BJM
Devienne,Karina F.; Raddi,Maria Stella G..
The microdilution technique, using a microplate photometer, to determine the minimal inhibitory concentration (MIC) for a natural product was compared to the serial tube dilution method. The MIC obtained for Paepalantine against S. aureus was the same by the two methods, showing an antimicrobial effect similar to chloramphenicol.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Microdilution; Spectrophotometric method.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200014
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Citrininotoxinogenicity of Penicillium spp. isolated from decaying apples BJM
Pepeljnjak,Stjepan; Šegvic,Maja; Ozegovic,Ladislav.
A study on the occurrence of citrinin and citrinin production ability of Penicillium spp. isolated from decaying apples collected from households in Croatia was carried out. Among 100 samples of apples, 37 strains of Penicillium spp. were found, including P. expansum, P. roqueforti, P. implicatum and P. purpurogenum. Citrinin production in liquid yeast medium by 11 strains of P. expansum varied in a range of 0.07 to 9.00 mg.kg-1. Citrinin was isolated from 19% of apple samples in range of 0.05 to 0.24 mg.kg-1. Antimicrobial activity of isolated citrinin, evaluated through tests on Bacillus subtilis, presented inhibitory zones varying from 5 mm to 1 cm. Minimal inhibitory concentrations (MIC) were 0.0072 µg.mL-1 for bacteriostatic effect, and 0.0144 µg.mL-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrinin; Penicillium expansum; Decaying apples; Antimicrobial activity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200007
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Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil BJM
Sartoratto,Adilson; Machado,Ana Lúcia M.; Delarmelina,Camila; Figueira,Glyn Mara; Duarte,Marta Cristina T.; Rehder,Vera Lúcia G..
Essential oils from aerial parts of Mentha piperita, M. spicata, Thymus vulgaris, Origanum vulgare, O. applii, Aloysia triphylla, Ocimum gratissimum, O. basilicum were obtained by steam destillation using a Clevenger-type system. These oils were screened for antibacterial and anti-Candida albicans activity using bioautographic method. Subsequently, minimal inhibitory concentration from oils was determined by microdilution method. Most essential oil studied were effective against Enterococcus faecium and Salmonella cholerasuis. Aloysia triphylla and O. basilicum presented moderate inhibition against Staphylococcus aureus while only A. tryphila and M. piperita were able to control the yeast Candida albicans. The oils were analyzed by GC and GC-MS techniques...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Medicinal plants; Antimicrobial activity; Minimal inhibitory concentration.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300001
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Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria BJM
Nascimento,Gislene G. F.; Locatelli,Juliana; Freitas,Paulo C.; Silva,Giuliana L..
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm), Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant extracts activity; Medicinal plants; Antimicrobial activity.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400003
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Antiviral activity of the Lippia graveolens (Mexican oregano) essential oil and its main compound carvacrol against human and animal viruses BJM
Pilau,Marciele Ribas; Alves,Sydney Hartz; Weiblen,Rudi; Arenhart,Sandra; Cueto,Ana Paula; Lovato,Luciane Teresinha.
Mexican oregano (Lippia graveolens) is a plant found in Mexico and Central America that is traditionally used as a medicinal herb. In the present study, we investigated the antiviral activity of the essential oil of Mexican oregano and its major component, carvacrol, against different human and animal viruses. The MTT test (3-4,5-dimethythiazol-2yl)-2,5-diphenyl tetrazolium bromide) was conducted to determine the selectivity index (SI) of the essential oil, which was equal to 13.1, 7.4, 10.8, 9.7, and 7.2 for acyclovir-resistant herpes simplex virus type 1 (ACVR-HHV-1), acyclovir-sensitive HHV-1, human respiratory syncytial virus (HRSV), bovine herpesvirus type 2 (BoHV-2), and bovine viral diarrhoea virus (BVDV), respectively. The human rotavirus (RV) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Verbenaceae; Antimicrobial activity; RNA virus; DNA virus; Condimental herbs.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400049
Registros recuperados: 104
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