|
|
|
| Registros recuperados: 57 | |
|
|
| Pellosi,Diogo S.; Batistela,Vagner R.; Souza,Vagner R. de; Scarminio,Ieda S.; Caetano,Wilker; Hioka,Noboru. |
| The development of drugs for photodynamic therapy (PDT) is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y) with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i) photosensitizers concentration, (ii) the LED irradiation time and (iii) the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemia salina; Factorial design; Photodynamic therapy; Bioassay; Xanthenes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401267 |
| |
|
|
| Bustos-Obregon,Eduardo; Vargas,Álvaro. |
| A chronic toxicity bioassay was conducted with the microcrustacean Artemia salina as the testing organism for the toxic organophosphate diazinon in order to determine if the species is an appropriate indicator of pollution in aquatic environments. Tests of animal exposure to different concentrations of the toxicant were performed for 24, 48 and 72 hours after larvae hatching. Registered mortality data was used to obtain the lethal dose 50 (LD50) of the organophosphate for each exposure time, considering the immobilization of A. salina larvae as the mortality parameter. The lethal concentration (LD50) in the same exposure times was calculated by evaluating morphological changes on the three initial stages of larval development. Both doses were determined by... |
| Tipo: Journal article |
Palavras-chave: Diazinon; Artemia salina; Ecotoxicology; Bioassay. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300013 |
| |
|
| |
|
|
| Ferreira,Maria Izildinha; Rodrigues,Geraldo Stachetti; Domingos,Marisa; Saldiva,Paulo Hilário do N.. |
| The Tradescantia stamen hair mutation assay (Trad-SHM) was employed for assessing the environmental mutagenesis in two areas of São Paulo City. The study sites were defined as follows: 1) Celso Garcia Avenue, an industrial area on the outskirts of the urban center (Belém neighborhood), with an average daily traffic volume of 8,750 vehicles; 2) Doutor Arnaldo Avenue, in downtown area (Pinheiros neighborhood), with a mostly residential and business offices occupation, with an average daily traffic volume of 46,125 vehicles. The negative control (background) site is located in the city of Jaguariúna, 138 km from São Paulo, in an area of low atmospheric pollution. The mutation frequency observed for Belém was similar to the background for clone 4430, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pollution; Mutagenesis; Bioassay; Tradescantia. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200017 |
| |
|
|
| Palacio,Soraya Moreno; Espinoza-Quiñones,Fernando Rodolfo; Galante,Raquel Manozzo; Zenatti,Dilcemara Cristina; Seolatto,Araceli Aparecida; Lorenz,Evandro Kleber; Zacarkim,Carlos Eduardo; Rossi,Nickeli; Rizzutto,Márcia de Almeida; Tabacniks,Manfredo Harri. |
| The present work was performed using the common onion (Allium cepa L.) as a bioindicator of toxicity of heavy metals in river water. The test waters were collected at two sampling sites: at the beginning and the end of the Toledo River. The bulbs of A. cepa L. were grown in test water with nine concentration levels of copper, zinc and lead from 0.1 to 50 ppm. In the laboratory, the influence of these test liquids on the root growth was examined during five days. For test liquids containing below 0.03-ppm dissolved Cu the root growth was reduced by 40% However, the same reduction occurred for 1-ppm dissolved Zn. For dissolved Pb, results reveal toxicity above 0.1 and 0.6 ppm at the beginning and the end of the Toledo river water, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute toxicity; Bioassay; Copper; Zinc; Lead; Allium cepa L. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400024 |
| |
|
| |
|
|
| Yai,Lucia Eiko Oishi; Vianna,Manoella Compostrini Barreto; Soares,Rodrigo Martins; Cortez,Adriana; Freire,Roberta Lemos; Richtznhain,Leonardo José; Gennari,Solange Maria. |
| The aim of the present experiment was to standardize a nested polymerase chain reaction (nPCR) for the detection of Toxoplasma gondii in tissues of experimentally infected pigs and to compare the performance of nPCR with the standard isolation technique, the bioassay in mice. Comparison between the two methods was done testing eight 4 month-old pigs orally inoculated with 5 x 10(4) oocysts of Toxoplasma gondii (AS-28 strain) and three non-infected pigs at the same age, kept as control. All animals were euthanatized 47 days after infection and samples of brain, heart, tongue and retina were collected from each animal for analysis by nPCR and bioassay in mice. By using the bioassay, Toxoplasma gondii was detected in 4 infected pigs, being two in the retina,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Pig; NestedPCR; Bioassay; Diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000300010 |
| |
|
| |
|
| |
|
|
| Destéfano,Ricardo Henry Rodrigues; Bechara,Ivanira José; Messias,Claudio Luiz; Piedrabuena,Aquiles Eugênico. |
| The study evaluated the effectiveness of Metarhizium anisopliae var. anisopliae (Hyphomycetes : Moniliales) strain E9, isolated from the pasture spittlebug Deois flavopicta (Hemiptera : Cercopidae), against larvae, prepupae and pupae stage and emergent adults of Anastrepha fraterculus, the South American fruitfly. The bioassay was carried out simulating field conditions, on autoclaved (AS) and non-autoclaved (NAS) soil from typical citrus orchards in the State of São Paulo, Southeastern region of Brazil. Various concentrations of conidia were incorporated into the soil the mortality, calculated based on the percentage of adult emergence, was 86% for the highest conidia concentrations: 2.52 x 10(10) for AS and 2.52 x 10(10) for NAS. The lethal concentration... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial control; Metarhizium anisopliae; Anastrepha fraterculus; Fruitfly; Bioassay. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100018 |
| |
|
|
| Petrolini,Fernanda Villas Boas; Lucarini,Rodrigo; Souza,Maria Gorete Mendes de; Pires,Regina Helena; Cunha,Wilson Roberto; Martins,Carlos Henrique Gomes. |
| In this study we evaluated the antibacterial activity of the crude hydroalcoholic extracts, fractions, and compounds of two plant species, namely Rosmarinus officinalis and Petroselinum crispum, against the bacteria that cause urinary tract infection. The microdilution method was used for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The crude hydroalcoholic extract of R. officinalis displayed in vitro activity against Gram-positive bacteria, with satisfactory MBC for the clinical isolate S. saprophyticus. The fractions and the pure compound rosmarinic acid did not furnish promising results for Gram-negative bacteria, whereas fractions 2, 3, and 4 gave encouraging results for Gram-positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibacterial activity; Bioassay; Rosmarinus officinalis; Petroselinum crispum; Urinary Tract Infections (UTI). |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300025 |
| |
|
| |
|
|
| Moreno,Andréia de Haro; Silva,Maria Fernanda Conz da; Salgado,Hérida Regina Nunes. |
| A stability study of azithromycin in ophthalmic preparations was developed by submission to different types of light, temperature and pH, using the biodiffusion assay (cylinder 3 x 3) for the quantifications. Bacillus subtilis, ATCC 9372, was used as test organism. The used concentration range was of 50 to 200 µg/mL. The study demonstrated that the drug suffered degradation when submitted to the ultraviolet light, germicide light, solar luminosity, acid solution, basic solution and hydrogen peroxide solution. The results were analyzed by the analysis of variance (ANOVA). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azithromycin/stability study; Bioassay; Photodegradation; Ophthalmic preparations. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200005 |
| |
|
|
| Silva,Francielle Santos da; Walter,Maurício Elesbão; Xavier,Bruna; Perobelli,Rafaela Ferreira; Calegari,Guilherme Zanini; Cardoso,Douglas Franco; Perlin,Valquiria Guedes; Dalmora,Sérgio Luiz. |
| Recombinant human interferon beta 1b (rhIFNβ-1b) is clinically used to treat multiple sclerosis. A reversed-phase liquid chromatography (RP-LC) method was carried out on a Jupiter C4 column (250 mm × 4.6 mm i.d.). The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water, and the mobile phase B was acetonitrile with 0.1% TFA run at a flow rate of 1.0 mL/min. A size exclusion liquid chromatography (SE-LC) method was carried out on a BioSep-SEC-S 2000 column (300 mm × 7.8 mm i.d.). The mobile phase consisted of 1 mM monobasic potassium phosphate, 8 mM sodium phosphate dibasic and 200 mM sodium chloride buffer pH 7.4, run isocratically at a flow rate of 0.8 mL/min. Retention times were 31.87 and 17.78 min, and calibration curves were linear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Recombinant human Interferon beta 1b; Biotechnology-derived medicine; Bioassay; Reversed-phase liquid chromatography; Size-exclusion liquid chromatography. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100571 |
| |
|
| |
|
| |
|
|
| Kreutz,Luiz Carlos; Barcellos,Leonardo José Gil; Silva,Tális Oliveira; Anziliero,Deniz; Martins,Daiana; Lorenson,Monique; Marteninghe,Ariane; Silva,Leonardo Bolognese da. |
| Toxicity risks of agricultural pesticides to fishes are pivotal. Currently, many questions remain unsolved regarding to the toxicity of commonly used pesticides to silver catfish (Rhamdia quelen), a South American catfish. The present studies have been designed to investigate the acute toxicity and the lethal concentration (LC50) of four herbicides, two fungicides and two insecticides to silver catfish fingerlings. All experiments were carried out in triplicates, in a static bioassay system, using commercially available pesticides. The data was analyzed through the Trimmed Spearman-Karber method available from the Environmental Protection Agency. The 96hLC50 and 95% lower and upper confidence limits, respectively, for the following pesticides were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioassay; Toxicity; Pesticides; LC50; Rhamdia quelen. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400022 |
| |
| Registros recuperados: 57 | |
|
|
|
