|
|
|
| Registros recuperados: 74 | |
|
|
| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
| |
|
| |
|
|
| Grizel, Henri. |
| The spread of major diseases of molluscs around the world and the increase of transfers need the development of adapted prophylactic strategies and the increase of research in epidemiology, immunology and genetics. The prophylactic strategies must be based on common laws with the establishment of a list of declarable pathogens, with basic sampling rules (sample size, frequency, definition of geographical areas) and with reglementation of the internal transfers. Moreover, specific adapted diagnosis should be developed to increase the sanitary control performance (e.g. ELISA test, DNA probe, cell culture). In the case of an infectious disease the epidemiology studies should be concerned, the relationship between the culture techniques and the disease, the... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Mollusc culture; Aquaculture techniques; Pathology; Immunology; Genetics; Epidemiology; Fish diseases; Disease control. |
| Ano: 1989 |
URL: http://archimer.ifremer.fr/doc/1989/acte-1483.pdf |
| |
|
|
| Gervais, Ophelie. |
| The flat oyster, Ostrea edulis, is the European endemic oyster species. Its production has been reduced because of overfishing and diseases including bonamiosis. Massive mortalities observed on the Pacific cupped oyster these last years explained the wish of shellfish farmers to diversify their production and their revival of interest for this patrimonial oyster species. However, bonamiosis due to the protozoan, Bonamia ostreae, is still a major problem for the production of this species. Measures to control the disease are limited and require a better knowledge of the interactions between the flat oyster and the parasite. Previous studies have suggested the involvement of apoptosis in flat oyster defense mechanisms against B. ostreae. This mechanism is... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Apoptose; Flat oyster; Ostrea edulis; Bonamia ostreae; Apoptosis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00370/48142/48243.pdf |
| |
|
| |
|
|
| Bedier, Edouard; Langlade, Aime; Angeri, Stéphane; Brizard, Raphael; Nerlovic, V.; Glize, Philippe; Haffray, Philippe. |
| Since the outbreaks of the parasitosis with Marteilia refringens and Bonamia ostreae in the last 60's, the French production of the European flat oyster dropped from 20000 mT to less than 1500 mT currently, leaving the French shellfish farming in situation of virtually monoculture of the Pacific cupped oyster Crassostrea gigas. To sustain this traditional culture, Ifremer has developed, at the experimental level, a genetic selection for a better tolerance to Bonamia ostreae which led to an improvement of survivals. The transfer of these results to the business scale requires the control of the reproduction in hatchery and the adaptation of the cycle of production to the constraints of seedlings in deep water as practised in Brittany (France). That's why a... |
| Tipo: Text |
Palavras-chave: Selection; Genetic; Bonamia ostreae; Parasite; Ostrea edulis; Oyster. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3313.pdf |
| |
|
|
| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
| |
|
| |
|
|
| Mourton, Chantal. |
| Oyster culture is, at a world scale, hit by variious epizooties, which are maintly caused by protozoans of the Phylum Ascetospora. Among them, Bonamia ostreae consitutes an appreciated model in order to study, at the cell scale, host-parasite relationships, as well as the immune role of hemocytes. Indeed, this parasite, which reproduces in hemocytes of Ostrea edulis, but cannot develop in Crassostea gigas. This development of an in vitro model for hemocyte-Bonamia relationships is presented. This includes the development and implementation of various techniques : making primocultures, protocol for infection with purified parasites, histological and immunoenzymatic analyses of infection. Such model allowed to study the interaction between host and... |
| Tipo: Text |
Palavras-chave: Modèle; Histologie; Méthodologie; Primoculture; Ostrea edulis; Parasite; Hémocytes; Bonamia ostreae; Ascetospora; Protozoaire; Epizootie. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/rapport-3234.pdf |
| |
|
| |
|
|
| Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle. |
| Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf |
| |
|
|
| Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie. |
| A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2... |
| Tipo: Text |
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf |
| |
|
| |
|
| |
|
|
| Goulletquer, Philippe. |
| Species invasion is considered as a main factor impacting the marine biodiversity (CBD, 2008). A comprehensive review of species invasion along the French Atlantic coastline is carried out to assess the present status and side effects resulting from those invasions. Vectors of introduction concerning voluntarily and involuntarily, legal & illegal case studies are reviewed to provide baseline information relating invasion success and potential control. Historical case studies regarding shellfish aquaculture-fisheries development and impacts of exotic diseases (e.g., Bonamia ostreae) as well as hitchhikers (e.g., Rapana venosa) are discussed. Among at least 150 reported exotic species, around 10 species have highly significant side-effects on the... |
| Tipo: Text |
Palavras-chave: Rapana venosa; Bonamia ostreae; Ruditapes philippinarum; Crepidula fornicata; Crasssostrea gigas; Species invasion; Marine biodiversity. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/acte-6828.pdf |
| |
|
|
| Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed. |
| Tipo: Text |
Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf |
| |
|
|
| Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan. |
| Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in... |
| Tipo: Text |
Palavras-chave: Intéraction; Hémocytes; Ostrea edulis; Bonamia ostreae; Parasitologie. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3366.pdf |
| |
|
|
| Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely... |
| Tipo: Text |
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf |
| |
|
|
| Bodoy, Alain; Bougrier, Serge; Geairon, Philippe; Garnier, Jacqueline; Boulo, Viviane; Heurtebise, Serge. |
| The flat oyster Ostrea edulis was originally reared in several regions of the European waters. The tidal saline ponds on the Atlantic coasts of France were highly productive before being hit by two consecutive epizooties. Parasitic diseases were described on this species, both of them leading 10 economic losses, as heavy mortalities have usually been recorded before the oysters reach the commercial size. Repeated experiments showed that the two diseases, Marteilia refringens and Bonamia ostreae are still active in this environment. Recently, some evidences were given that haemocytoblasts of Crassostrea gigas had the ability to lyze the Bonamia cells. Therefore, in an experiment of contiguous culture of the two species of oysters, the growth, mortality and... |
| Tipo: Text |
Palavras-chave: Molluscs; Mortalities; Growth; Pathology; Crassostra gigas; Ostrea edulis; Marteilia refringens; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-3049.pdf |
| |
|
| |
| Registros recuperados: 74 | |
|
|
|
