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| Registros recuperados: 101 | |
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| Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia. |
| Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other... |
| Tipo: Text |
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf |
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| Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.. |
| Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337 |
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| Rosa,Thuanny Lins Monteiro; Araujo,Caroline Palacio de; Alexandre,Rodrigo Sobreira; Schmildt,Edilson Romais; Lopes,José Carlos. |
| Abstract: The objective of this work was to evaluate the biometry of fruit and seed, and the diversity of paradise nut (Lecythis pisonis) genotypes. Fruit of 12 paradise nut genotypes were collected from plants in the northern region of the state of Espírito Santo and in the eastern region of the state of Minas Gerais, Brazil. Data were subjected to the analysis of variance, and means of the different genotypes were compared by Scott-Knott’s mean clustering test. The phenotypic diversity was analyzed using Mahalanobis’ generalized distance, and clustering, by Tocher’s optimization method and the graphical dispersion of canonical variables. The relative contribution of the characters to diversity was assessed by Singh’s method. The paradise nut genotypes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lecythis pisonis; Characterization; Divergence; Genotypes; Sapucaia nut. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102502 |
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| BELLÉ,C.; KASPARY,T.E.; BALARDIN,R.R.; RAMOS,R.F.; ANTONIOLLI,Z.I.. |
| ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Root-knot nematode; Weed plants; Isozyme esterase; Characterization; Hostability. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100292 |
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| Correa, Cynthia Candida; Alves, Alexandre Florindo. |
| O uso dos produtos à base de plantas medicinais surge como uma forte tendência mundial. Esse é considerado um mercado promissor e apresenta números expressivos, tornando-se um ambiente de negócio atrativo. Entretanto, apesar de toda importância social e econômica, o Brasil e o Paraná não tem investido adequadamente na produção, industrialização e distribuição dessas plantas. Nesse contexto, buscou-se, através desse trabalho, caracterizar e analisar a produção de plantas medicinais no estado do Paraná. Para alcance desse objetivo foram utilizados dados secundários, encontrados em livros, artigos científicos, revistas e, principalmente, em sites governamentais. Pode-se concluir, a partir da pesquisa, que existe um aumento da oferta e demanda de plantas... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Plantas medicinais; Sistema produtivo; Caracterização; Paraná; Medicinal plants; Productive system; Characterization; Paraná state - Brazil; Health Economics and Policy. |
| Ano: 2008 |
URL: http://purl.umn.edu/108079 |
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| Carvalho, Daniela Moreira de; Souza, Jalmir Pinheiro De. |
| A caprino-ovinocultura na região Nordeste tem grande importância tanto econômica quanto social, devido ao fato dos animais terem uma boa adaptação ao clima e a alimentação, muitas vezes escassa, disponível na região. A compreensão das cadeias produtivas locais permite a proposição de melhorias no setor a fim de torná-lo mais eficiente. Na caprino-ovinocultura a eficiência ocorre através da oferta aos consumidores de carnes de melhor qualidade, animais jovens, constância de oferta, segurança alimentar e preços competitivos. O resultado da eficiência do sistema produtivo seria de uma maior rentabilidade, uma inserção em novos mercados, ou seja, numa maior competitividade de todo setor. Nesse sentido objetivou-se realizar uma análise descritiva da cadeia... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Caprino-ovinocultura; Cadeia produtiva; Coordenação; Caracterização; Garanhuns; Sheep & goat farming; Productive chain; Coordination; Characterization; Garanhuns; Livestock Production/Industries. |
| Ano: 2008 |
URL: http://purl.umn.edu/109705 |
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| Maia,Clara Yohana; Soares,Nazaré Suziane; Castro,Ana Cecília Ribeiro; Queirós,João Ravelly Alves de; Aragão,Fernando Antonio Souza de; Bordallo,Patricia do Nascimento. |
| ABSTRACT Anthurium affine is an ornamental Brazilian native plant very appreciated due to its long-lasting durability. To explore the genetic diversity available, 21 A. affine half-sibling progeny were evaluated using morphoagronomic and molecular descriptors. The morphoagronomic descriptors used were: plant height, plant diameter, growth habit, leaf number, leaf blade length, leaf blade width, petiole length, petiole diameter, leaf margin undulation, general appearance; soil cover rate and Random Amplified Polymorphic DNA (RAPD) markers the molecular one. All the descriptors within the progeny showed variability and general appearance, leaf margin undulation and soil cover rate are the ones that most contribute to the plants divergence. From 45 RAPD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clustering; Characterization; Genetic variation; Ornamental; RAPD. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400410 |
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| Otero,Laura; Ducasse,Daniel; Miller,Robert N. G.. |
| Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Olea europaea; Molecular markers; Characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015 |
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| FIGUEIRA,Luanda de Paula; SOARES,Fabiane Veloso; JÚNIOR,Roberto Daibes NAIFF; VINHOTE-SILVA,Alana Cristina; SILVA,Susi Simas da; ESPIR,Thaís Tibery; NAIFF,Maricleide de Farias; GOMES,Luis Henrique Monteiro; MOREIRA,Flávia Regina Almeida Campos Naief; FRANCO,Antonia Maria Ramos. |
| ABSTRACT Few cases of human cutaneous leishmaniasis (CL) caused by Leishmania naiffi were described in the medical literature. The aim of this study was to report and analyze new cases of L. naiffi in the period between the years 1992 to 2011. The strains were characterized by isoenzyme analysis. All patients assisted had small lesions; ranging from 1.0x1.0 mm and 13.5x11.5 mm. The lesions observed were widely distributed: 55.5% on the lower limb, 5.5% in the abdominal area, 16.6% on upper limb and 22.2% in upper limb and back. Seventy-two percent of patients had ulcerated lesions. Clinical course of the disease varied from 1 to 10 months. According to gender, most infected individuals were men (83.3%). The patients came from Amazonas (10), Pará (01) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: American Tegumentary Leishmaniasis; Leishmania naiffi; Characterization; Experimental stations; Lutzomyia. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100047 |
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| Andrade, Renata Aparecida de; UNESP - Jaboticabal/SP; Lemos, Eliana Gertrudes de Macedo; UNESP - Jaboticabal/SP; Martins, Antonio Baldo Geraldo; UNESP - Jaboticabal/SP; Paula, Rinaldo César de; UNESP - Jaboticabal/SP. |
| Em certas espécies vegetais, a distinção entre variedades pode ser realizada com base em aspectos morfológicos das plantas, o que permite a identificação mesmo quando não apresentam flores e/ou frutos. Diante disso, realizou-se o presente trabalho cujo objetivo foi a caracterização morfológica e o agrupamento de plantas de rambutan. Foram avaliados: altura da planta (cm), diâmetro e geometria da copa (entre linhas e entre plantas, em cm), diâmetro do tronco (cm) e disposição dos ramos no tronco, tamanho de folhas e folíolos (comprimento e largura, em cm), número de folíolos, comprimento da ráquis (cm), comprimento do pecíolo (cm), área foliar (cm2), vigor das plantas, sensibilidade ao frio, formato dos folíolos, pilosidade, coloração dos folíolos (faces... |
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Palavras-chave: 5.01.03.04-0 Produção de Mudas Nephelium lappaceum; Morfologia; Caracterização fitotecnia Nephelium lappaceum; Morphology; Characterization. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/890 |
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| Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG. |
| Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit... |
| Tipo: Pesquisa laboratorial |
Palavras-chave: 5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276 |
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| Miranda, Ordilena Ferreira de; de Souza, Saulo Eduardo Xavier Franco; Milan, Rodrigo José; Bueno, Aline Borges; Almeida, Marcilio de. |
| Psychotria viridis Ruiz & Pav. (Rubiaceae) occurs naturally throughout the Amazon and it is traditionally used by indigenous communities, being incorporated into religious use in urban contexts over the last few decades. It is known and cultivated in many regions of South America for possessing valuable bioactive alkaloids. In this paper, we described P. viridis leaf morphology, anatomy and histochemistry from three populations cultivated in the southeastern Brazil, in order to identify possible adaptations to local environment and management. All plants presented terminal stipules and basic morpho-anatomical patterns of leaves, consistent with most species of the genus, as heterogeneous dorsiventral mesophyll, uniseriate epidermis, presents large... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Characterization; Alkaloid; Trichomes; Glandular duct; Domatia. characterization; Alkaloid; Trichomes; Secretory duct; Domatia.. |
| Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/50369 |
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| Peixoto, Ferdyanne Beatriz Sabóia; Peixoto, Jean Charles da Cunha; Assunção, Enedina Nogueira de; Peixoto, Elise Miranda; Pereira, José Odair; Astolfi-Filho, Spartaco. |
| Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbiologia 2.12.00.00-9 biodegradation; Characterization; DCPIP; Antimicrobial resistance. Microbiologia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223 |
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| I., Manta H.; D., James D.; I., Awu J.. |
| Green house effect and ozone layer depletion due continuous environmental pollution from fossil fuel has speed up the quest for search of alternative energy fuel. In recent year, biofouls has attracted much attention as a reliable substitute to fossil fuel. The objective of this study was to evaluate and characterize biofuel produced from Egusi vegetable oil by applying standard method of transesterification. This study was carried out at Processing and Storage Engineering Departmental (PSED) laboratory of National Centre for Agricultural Mechanization (NCAM), Ilorin Kwara State, Nigeria. The results of the proximate characterization of the produced Egusi vegetable oil shows crude protein, crude fibre, ether extract, moisture content, ash content and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egusi Vegetable Oil; Characterization; Transesterification; Methyl Esters; Biofuel. |
| Ano: 2021 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6205 |
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| Registros recuperados: 101 | |
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