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Exigência líquida de zinco, cobre e ferro para cordeiros em pastejo no semiárido - doi: 10.4025/actascianimsci.v32i3.5883 Animal Sciences
Mendes, Rodrigo de Souza; UFCG; Silva, Aderbal Marcos de Azevêdo; UFCG; Silva, Guilherme de Lira Sobral; UFCG; Nóbrega, Giovanna Henriques da; UFCG; Lôbo, Katiuscia Menezes; UFCG; Pereira Filho, José Morais; UFCG.
Este trabalho teve por objetivo determinar as exigências líquidas de zinco (Zn), cobre (Cu) e ferro (Fe) de cordeiros Santa Inês em pastejo na região semiárida. Os animais com peso vivo entre 20 e 30 kg apresentaram conteúdo corporal no peso de corpo vazio (PCV) para esses minerais de 60,26 a 76,37 mg kg-1, 10,78 a 16,72 mg kg-1 e 133,44 a 126,98 mg kg-1, respectivamente. Observou-se que com o aumento do PCV, as concentrações de Cu e Zn também aumentavam, enquanto a de Fe diminuía. As exigências líquidas para cordeiros em regime de pastejo variaram de 1,69 a 7,86 mg dia-1 para Cu e 7,20 a 27,4 mg dia-1 para Zn. Os resultados obtidos no presente trabalho, para exigências desses minerais, foram maiores do que as exigências dietéticas recomendadas pelo do NRC...
Tipo: Pesquisa de campo Palavras-chave: Zootecnia composição corporal; Exigências nutricionais; Ferro; Cobre; Zinco; Exigências dietéticas Zootecnia body composition; Nutritional requirements; Iron; Copper; Zinc; Dietary requirements.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/5883
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Evaluation of the potential of Pontederia parviflora Alexander in the absorption of copper (Cu) and its effects on tissues - doi: 10.4025/actascibiolsci.v32i3.3940 Biological Sciences
Balassa, Graziela Custodio; Universidade Tecnológica Federal do Paraná; Souza, Débora Cristina de; Universidade Tecnológica Federal do Paraná - Campus Campo Mourão; Lima, Sônia Barbosa de; Universidade Tecnológica Federal do Paraná - Campus Campo Mourão.
The study sought to evaluate the potential of the aquatic macrophyte Pontederia parviflora Alexander in the absorption of copper (Cu) and possible variations in its tissues after 21 days of exposure to this metal. The concentration of Cu was analyzed in the solution and in the vegetal tissues (stem, root and leaves). The experiment was set up in triplicates with weekly measurements of pH, temperature and DO. The results indicated that P. parviflora reduced 96% of Cu in the solution and the root was the vegetal tissue, which accumulated more Cu. Values of pH, DO and temperature were in accordance with the metabolic activities of the plants. In the anatomic analyses, dark spots were identified in the vascular bundles and in the epidermis of the aerial parts,...
Palavras-chave: 2.05.03.00-8 aquatic macrophyte; Phytoremediation; Copper Ecologia aplicada aquatic macrophyte; Phytoremediation; Copper.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3940
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Copper Stress on Photosynthesis of Black Mangle (Avicennia germinans) Anais da ABC (AABC)
GONZALEZ-MENDOZA,DANIEL; GIL,FRANCISCO ESPADAS y; ESCOBOZA-GARCIA,FERNANDO; SANTAMARIA,JORGE M.; ZAPATA-PEREZ,OMAR.
The effects of copper toxicity on the photosynthetic activities of Avicennia germinans was investigated using two CuSO4 concentrations (0.062 and 0.33 M) added in Hoagland's solution in an aerated hydroponic system. Photosynthesis and chlorophyll fluorescence were measured after 30 h of copper stress. Results obtained in this study show that increasing levels of Cu+2 of 0.062 and 0.33 M Cu+2 resulted in a general reduction of the stomatal conductance (28 and 18%, respectively) and 100% of inhibition of net photosynthesis. Additionally, at these concentrations of Cu+2, reductions of chlorophyll fluorescence parameters were also observed. These changes suggested that the photosynthetic apparatus of Avicennia germinans was the primary target of the Cu+2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicennia germinans; Chlorophyll fluorescence; Copper; Photosystem II; Photosynthesis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200665
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The functional links between prion protein and copper Biol. Res.
VARELA-NALLAR,LORENA; TOLEDO,ENRIQUE M; CHACÓN,MARCELO A; INESTROSA,NIBALDO C.
Prion diseases are fatal neurodegenerative disorders associated with the conversion of the cellular prion protein (PrPC) into a pathologic isoform. Although the physiological function of PrPC remains unknown, evidence relates PrPC to copper metabolism and oxidative stress as suggested by its copper-binding properties in the N-terminal octapeptide repeat region. This region also reduces copper ions in vitro, and this reduction ability is associated with the neuroprotection exerted by the octarepeat region against copper in vivo. In addition, the promoter region of the PrPC gene contains putative metal response elements suggesting it may be regulated by heavy metals. Here we address some of the evidence that support a physiological link between PrPC and...
Tipo: Journal article Palavras-chave: Prion protein; Copper; Heparin; HSPG; Prnp; MRE.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100005
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Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium Biol. Res.
POLANCO,RUBÉN; CANESSA,PAULO; RIVAS,ALEXIS; LARRONDO,LUIS F; LOBOS,SERGIO; VICUÑA,RAFAEL.
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1...
Tipo: Journal article Palavras-chave: Acel; Basidiomycete; Copper; Phanerochaete chrysosporium.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007
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Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers Biol. Res.
LINDER,MARIA C; MORIYA,MIZUE; WHON,ANNE; KASSA,AZEB; GILLEY,CYNTHIA.
Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed...
Tipo: Journal article Palavras-chave: Intestinal iron absorption; Caco2 cells; Apotransferrin; Vesicular transport; Copper; Manganese.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016
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Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae Biol. Res.
Hernandez,Pedro P; Undurraga,Cristian; Gallardo,Viviana E; Mackenzie,Natalia; Allende,Miguel L; Reyes,Ariel E.
Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae...
Tipo: Journal article Palavras-chave: Acute exposure; Apoptosis; Copper; Early life stage; Heat shock; Protein-70; Zebrafish.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100002
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Divalent cations as modulators of neuronal excitability: Emphasis on copper and zinc Biol. Res.
DELGADO,RICARDO; VERGARA,CECILIA; WOLFF,DANIEL.
Based on indirect evidence, a role for synaptically released copper and zinc as modulators of neuronal activity has been proposed. To test this proposal directly, we studied the effect of copper, zinc, and other divalent cations on voltage-dependent currents in dissociated toad olfactory neurons and on their firing rate induced by small depolarizing currents. Divalent cations in the nanomolar range sped up the activation kinetics and increased the amplitude of the inward sodium current. In the micromolar range, they caused a dose dependent inhibition of the inward Na+ and Ca2+ currents (I Na and I Ca) and reduced de amplitude of the Ca2+-dependent K+ outward current (I Ca-K). On the other hand, the firing rate of olfactory neurons increased when exposed to...
Tipo: Journal article Palavras-chave: Copper; Zinc; Modulators; Channels; Neuronal excitability.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100019
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Optimization of biomass production with copper bioaccumulation by yeasts in submerged fermentation BABT
Arakaki,Andréa Haruko; Souza Vandenberghe,Luciana Porto de; Soccol,Vanete Thomaz; Masaki,Ryu; Rosa Filho,Ernani Francisco da; Gregório,Alexsandro; Soccol,Carlos Ricardo.
The objective of this work was to study the production of biomass with copper bioaccumulation in submerged fermentation using sugarcane molasses. Candida pelliculosa BARU 05 isolated from Baru (Dipteryx alata) was selected for its good capacity to accumulate the copper. Fermentation was carried out using the medium composed by sugarcane molasses at 5 °Brix enriched with (g/L) CuSO4.5H2O 0.1; yeast extract, 10.0; (NH4)2SO4, 5.0 ; KH2PO4, 5.0 MgSO4, 0.5, inoculum 10 % of total volume (100 ml), pH 6.0, and incubation at 30 °C, 120 rpm for 120 h. After three steps of optimization an uptake of 95.04% and 13.397 g/L biomass were obtained. The kinetics of copper bioaccumulation and biomass production was followed in a 10- liter bioreactor in a batch and fed-batch...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioaccumulation; Baru; Yeast; Copper; Sugarcane molasses.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500021
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Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation BABT
Tychanowicz,Giovana Kirst; Souza,Daniela F. de; Souza,Cristina G. M.; Kadowaki,Marina Kimiko; Peralta,Rosane Marina.
Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Laccase; Pleurotus pulmonarius; Phenoloxidase; Solid state fermentation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600002
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Sobre a Dosagem Fotométrica do Cobre em Águas BABT
Spitzner,Reinaldo.
The author presents an analytic process for copper quantitative determination in mineral and potable waters, based in the reaction of the cation copper with sodium diethyl-dithio carbamate. Due to the presence of certain elements common in water, such as iron, calcium, magnesium, etc., the main principles of the reactions behavior were studied. In the same manner the reaction sensibility was verified, as well as its precision, by the addition of known quantities of copper. About the end of the work presented, there are shown some copper determinations made through the mentioned process with mineral and potable waters of Paraná.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Photometry; Mineral waters.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000500004
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Correlation between heavy metal ions (copper, zinc, lead) concentrations and root length of Allium cepa L. in polluted river water BABT
Palacio,Soraya Moreno; Espinoza-Quiñones,Fernando Rodolfo; Galante,Raquel Manozzo; Zenatti,Dilcemara Cristina; Seolatto,Araceli Aparecida; Lorenz,Evandro Kleber; Zacarkim,Carlos Eduardo; Rossi,Nickeli; Rizzutto,Márcia de Almeida; Tabacniks,Manfredo Harri.
The present work was performed using the common onion (Allium cepa L.) as a bioindicator of toxicity of heavy metals in river water. The test waters were collected at two sampling sites: at the beginning and the end of the Toledo River. The bulbs of A. cepa L. were grown in test water with nine concentration levels of copper, zinc and lead from 0.1 to 50 ppm. In the laboratory, the influence of these test liquids on the root growth was examined during five days. For test liquids containing below 0.03-ppm dissolved Cu the root growth was reduced by 40% However, the same reduction occurred for 1-ppm dissolved Zn. For dissolved Pb, results reveal toxicity above 0.1 and 0.6 ppm at the beginning and the end of the Toledo river water, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute toxicity; Bioassay; Copper; Zinc; Lead; Allium cepa L.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400024
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Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania) BABT
Aliko,Valbona; Hajdaraj,Gerta; Caci,Atenisa; Faggio,Caterina.
ABSTRACTDestabilisation of blood cell lysosomes in Mediterranean green crabCarcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 2014 were exposed in the laboratory to sub-lethal, environmentally realistic concentrations of copper. Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p<0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p<0.05) than in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcinus aestuarii; Neutral red retention assay; Lysosomal membrane destabilization; Bioindicator; Copper.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500750
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Distribution of copper in soil and rice system of Hainan Island, China BABT
Dengfeng,WANG; Haijie,HUANG; Huande,FENG; Hua,WANG; Miaojie,HAN; Zhiping,QI.
ABSTRACT This research aimed to discover the distribution and the primary influence factors of Cu in the soil-rice systems of tropical farmland. Soil samples of farmland and rice plant (stalk and polished rice) from the western region of Hainan Island were collected and studied. The results showed that the average Cu content in the topsoil of the study area was 15.75 mg kg-1; the highest Cu content (45.92 mg kg-1) was found in the rice fields of the northern area, where pyroclastic parent material is distributed. Thus, there is a potential for Cu contamination of the rice grown in this region. The average contents of Cu in the rice stalks and polished rice were 16.9 and 5.68 mg kg-1, respectively, indicating that the stalks had a larger capacity for Cu...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rice; Copper; Arable land; Hainan.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200703
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Insights into the physiological function of cellular prion protein BJMBR
Martins,V.R.; Mercadante,A.F.; Cabral,A.L.B.; Freitas,A.R.O.; Castro,R.M.R.P.S..
Prions have been extensively studied since they represent a new class of infectious agents in which a protein, PrPsc (prion scrapie), appears to be the sole component of the infectious particle. They are responsible for transmissible spongiform encephalopathies, which affect both humans and animals. The mechanism of disease propagation is well understood and involves the interaction of PrPsc with its cellular isoform (PrPc) and subsequently abnormal structural conversion of the latter. PrPc is a glycoprotein anchored on the cell surface by a glycosylphosphatidylinositol moiety and expressed in most cell types but mainly in neurons. Prion diseases have been associated with the accumulation of the abnormally folded protein and its neurotoxic effects;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PrPc; Cellular function; Transmissible spongiform encephalopathies; Laminin; Signal transduction; Copper.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500005
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Copper influence on polyphosphate metabolism of Cunninghamella elegans BJM
Souza,Patrícia Mendes de; Marinho,Petrusk Homero; Lima,Marcos Antônio Barbosa de; Nascimento,Aline Elesbão do; Takaki,Galba Maria de Campos.
The aim of this work was to evaluate the physiological aspects of polyphosphate metabolism of Cunninghamella elegans grown in presence of copper. The growth profile was obtained by means of biomass yields, orthophosphate consumption, polyphosphate accumulation and phosphatases activities. The results revealed the influence of copper on the growth, observed by biomass yields. Orthophosphate consumption was faster in cells grown in the presence of copper. The presence of copper in the culture medium induced polyphosphate accumulation. The polyphosphate level was almost constant in the beginning of control culture growth, and could be related to the exponential growth phase. On the other hand, the copper treated cultures exhibited a significant reduction in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cunninghamella elegans; Copper; Polyphosphate; Phosphatases.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400002
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Relationship between Paratuberculosis and the microelements Copper, Zinc, Iron, Selenium and Molybdenum in Beef Cattle BJM
Paolicchi,F.; Perea,J.; Cseh,S.; Morsella,C..
To study the deficiency of minerals and its relationship with Paratuberculosis, blood, serum, and fecal samples were obtained from 75 adult bovines without clinical symptoms of the disease and from two bovines with clinical symptoms of the disease, from two beef herds with a previous history of Paratuberculosis in the Province of Buenos Aires, Argentina. Serum samples were processed by ELISA and feces were cultured in Herrolds medium. Copper, zinc and iron in serum were quantified by spectrophotometry and selenium was measured by the activity of glutathione peroxidase. We also determined copper, zinc, iron and molybdenum concentrations in pastures and the concentration of sulfate in water. Mycobacterium avium subsp paratuberculosis (Map) was isolated from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paratuberculosis; Micronutrients; Selenium; Molybdenum; Copper.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100022
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Changes in nitrate reductase activity and oxidative stress response in the moss Polytrichum commune subjected to chromium, copper and zinc phytotoxicity Braz. J. Plant Physiol.
Panda,Sanjib Kumar; Choudhury,Shuvasish.
The main aim of this paper was to investigate the effect of chromium (Cr), copper (Cu) and zinc (Zn) on nitrate reductase (NR) activity and oxidative stress responses in the moss Polytrichum commune. Cr, Cu and Zn resulted in the inhibition of NR activity. A decline in total chlorophyll content was observed after 24 and 48 h of metal treatment. Accumulation of the metals showed a dose and time dependent increase. High accumulation of Cu, Cr and Zn were seen in moss shoots after 24 and 48 h of treatment. Treatment of Cr, Cu and Zn for 24 or 48 h resulted in the increase of malondialdehyde (MDA) content in moss shoots. The highest increase was observed in shoots under Cu treatment followed by Cr and Zn. The MDA content was significantly higher after 48h....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Chromium; Copper; Malondialdehyde; Nitrate reductase; Oxidative stress; Polytrichum commune; Zinc.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200001
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Changes induced by Cu2+ and Cr6+ metal stress in polyamines, auxins, abscisic acid titers and antioxidative enzymes activities of radish seedlings Braz. J. Plant Physiol.
Choudhary,Sikander P.; Bhardwaj,Renu; Gupta,Bishan D.; Dutt,Prabhu; Gupta,Rajinder K.; Kanwar,Mukesh; Dutt,Pitamber.
The present study determined the effects of copper and chromium metals on the endogenous titers of polyamines, auxins, abscisic acid and antioxidative enzyme activities in Raphanus sativus L. cv. Pusa chetki seedlings. Among polyamines, putrescine and spermidine contents were enhanced by Cu2+ metal to 62.44 and 402.8 µg g-1 f.w. respectively over control. Spermine which was not observed in control was recorded in highest concentration (1287.9 µg g-1 f.w.) in Cu2+ metal stressed seedlings. On the other hand Cr6+ metal treated seedlings showed reduced contents of putrescine (1.43 µg g-1 f.w.), cadaverine (0.09 µg g-1 f.w.), spermidine (277.99 µg g-1 f.w.) and spermine (2.29 µg g-1 f.w.) when compared to control and Cu2+ metal treated seedlings. Significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Chromium; Copper; Guaiacol peroxidase; Polyamines (PAs); Superoxide dismutase.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400006
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Transport and detoxification of manganese and copper in plants Braz. J. Plant Physiol.
Ducic,Tanja; Polle,Andrea.
Heavy metals like Mn and Cu, though essential for normal plant growth and development, can be toxic when present in excess in the environment. For normal plant growth maintenance of metal homeostasis is important. Excess uptake of redox active elements causes oxidative destruction. Thus, uptake, transport and distribution within the plant must be strongly controlled. Regulation includes precisely targeted transport from the macro-level of the tissue to the micro-level of the cell and organelles. Membrane transport systems play very important roles in metal trafficking. This review provides a broad overview of the long distance and cellular transport as well as detoxification and homeostasis mechanisms of Mn and Cu, which are essential micronutrients but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Heavy metals; Manganese; Phytoremediation; Transport.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100009
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