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Registros recuperados: 93
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The potential of near infrared spectroscopy (NIRS) to measure the chemical composition of aquaculture solid waste ArchiMer
Lopes Galasso, Helena; Callier, Myriam; Bastianelli, Denis; Blancheton, Jean-paul; Aliaume, Catherine.
In aquaculture, it is extremely important to determine the composition of fish farm waste to evaluate its potential impacts and to improve its reuse. Near-infra red spectroscopy (NIRS), an alternative to standard chemical analytical techniques, is a quick non-invasive method to assess physical and chemical composition, reducing the cost of routine analysis. We developed NIRS calibration models for organic matter (OM), total organic carbon (TOC), total organic nitrogen (TON), the carbon/nitrogen ratio (C/N), total phosphorus (TP) and the lipid content of marine fish particulate waste. To obtain a wide range of compositions of fish waste, decomposition time, feed loss, and inter-specific variations were taken into account. The NIRS calibration models were...
Tipo: Text Palavras-chave: Near infrared spectroscopy; Fish farm waste; Faeces; Dicentrarchus labrax; Chemical composition.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00382/49369/49771.pdf
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Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility ArchiMer
Abascal, F; Cosson, J; Fauvel, Christian.
Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating...
Tipo: Text Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf
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Rickettsia-like organism infecting juvenile sea-bass Dicentrarchus labrax ArchiMer
Comps, Michel; Raymond, J. C.; Plassiart, G. N..
High levels of mortalities (20%) associated with abnormal swimming have been observed in farmed juveniles of seabass, Dicentrarchus labrax, in Mediterranean coast, southern France. Histopathology was obvious in the brain with nervOUS tissue necrosis and the presence of ceUs containing basophilic granules corresponding to smaU coccoid organisms. Ultrastructural examination revealed these organisms were rickettsia-like organisms morphologicaUy close to Piscirickettsia salmonis
Tipo: Text Palavras-chave: Pathology; Dicentrarchus labrax; Sea Bass; Rickettsia like.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-6158.PDF
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Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax). ArchiMer
Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax...
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
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L'activite de deplacement et le reperage de la nourriture chez le bar juvenile captif ArchiMer
Anthouard, M; Paris, J.
Movement activity and localization of food were studied dynamically with the objective of establishing the capacities of behavioral adaptation of the young Dicentrarchus labrax faced with a novel situation. The role played by previous rearing conditions was demonstrated, the level of stimulation being a sensitive intervening factor (currents associated with the food). The data show the favourable effects of such treatment, and are in agreement with work carried out on higher vertebrates, which have brought to light the importance of early stimulation in behavioral ontogeny which respect to emotionality as well as capacities of adaptation in subjects reaching adulthood.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Juveniles; Stimuli; Locating; Locomotion; Fish culture; Nutrition; Ethology.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1253.pdf
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Nutritional programming by dietary carbohydrates in European sea bass larvae: not always what expected at juvenile stage ArchiMer
Zambonino-infante, Jose-luis; Panserat, S.; Servili, Arianna; Mouchel, Olivier; Madec, Lauriane; Mazurais, David.
Optimizing the metabolic use of these carbohydrate-rich plant products in fish feeds could be achieved through nutritional programming strategies of fish larvae. The present study aims at testing the long-term effect of an early-life high-carbohydrate nutritional (34% of starch in the feed) stimulus in European sea bass (Dicentrarchus labrax), by examining several biochemical and molecular parameters involved in carbohydrate metabolism in sea bass larvae and juveniles, compared to a control group fed standard larval regime (phase 1). As awaited, specific activities of digestive enzymes, from pancreatic and intestinal segments, involved in glucide digestion (amylase, sucrase) were enhanced together with an up-regulation of glucokinase gene and...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Glucose metabolism; High-carbohydrate regimes; Marine fish larvae; Nutritional programming.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00470/58129/60552.pdf
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Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla) ArchiMer
Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno.
The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant...
Tipo: Text Palavras-chave: Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-411.pdf
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Body shape variation in meiotic gynogenetic and triploid sea bass, Dicentrarchus labrax ArchiMer
Peruzzi, Stefano; Wiborg-dahle, Stine; Haffray, Pierrick; Chatain, Beatrice; Primicerio, Raul.
A method of geometric morphometrics was used to characterize body shape variation in meiotic gynogenetic and triploid sea bass in comparison with diploid controls. A total of 13 coordinate landmark data were recorded on the profile of 34-month-old control (n = 81), gynogenetic (n = 117) and triploid (n = 131) siblings generated from two females (A and B). Three main axes of morphometric variation were consistent, and these accounted for 60.8 and 57.6% of the total variability in females A and B, respectively. There were significant shape deviations of triploid and gynogenetic fish relative to the controls, and differences were particularly evident in the progeny of one female. Overall, triploids displayed a more slender shape than diploids, whereas...
Tipo: Text Palavras-chave: Body shape variability; Morphometry; Geometry of Procrustes distance; Triploidy; Gynogenesis; European sea bass; Dicentrarchus labrax.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00025/13651/10757.pdf
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Estimates of heritability and genotype-environment interactions for body weight in sea bass (Dicentrarchus labrax L.) raised under communal rearing conditions ArchiMer
Saillant, Eric; Dupont Nivet, Mathilde; Haffray, Pierrick; Chatain, Beatrice.
Genetic parameters for somatic growth rate in sea bass were estimated in two experiments where 27 (year-class 1997) to 30 (year-class 1998) families of sea bass (3 dams mated with 9-10 sires according to a full factorial design) were raised mixed in the same tanks starting shortly before hatching (48 h post fertilization). Trials were performed under two temperature (experiment 1, 1997: group high temperature (HT) and group low temperature (LT)) and two density regimes (experiment 2, 1998: group high density (HD) and group low density (LD)) with a subset of the mating design (18 families) being reproduced in the two experiments. All the fish were genotyped at 3-6 microsatellite loci which allowed them to be assigned to the individual breeders used in the...
Tipo: Text Palavras-chave: Genotype x environment interaction; Communal rearing; Microsatellites markers; Dicentrarchus labrax; Growth; Heritability.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1706.pdf
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Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions ArchiMer
Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied....
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf
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Conséquences à long terme d'une exposition précose à l'hypoxie sur la physiologie du bar européen (Dicentrarchus labrax) et de la sole commune (Solea solea) ArchiMer
Vanderplancke, Gwenaelle.
The dissolved oxygen in seawater has steadily declined in many coastal marine ecosystems in the last 50 years because of global warming and increasing coastal human activities. This decrease in oxygen in seawater causes episodes of hypoxia increasingly frequent and severe in coastal and estuarine areas. Some organisms may escape these environmental constraints by migration or dispersion. Others, with limited swimming capabilities must implement physiological regulation (phenotypic plasticity) to enable them to adapt and survive. This is particularly the case of marine fish larvae that are likely to be present in coastal nursery at the end of their development. It is now well established that the regulations implemented by organisms to cope with their...
Tipo: Text Palavras-chave: Hypoxie; Conditionnement; Programmation environnementale; Poissons marins; Changement climatique; Oxygène; Dicentrarchus labrax; Solea solea; Marine fish; Climate change; Oxygen; Hypoxia; Conditioning; Environmental programming.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00498/60938/64332.pdf
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New insights into behavioural ecology of European seabass off the West Coast of France: implications at local and population scales ArchiMer
De Pontual, Helene; Lalire, Maxime; Fablet, Ronan; Laspougeas, Claire; Garren, Francois; Martin, Stephane; Drogou, Mickael; Woillez, Mathieu.
From 2010 to 2012, 246 data storage tags were deployed on European seabass in the Iroise Natural Marine Park, a marine protected area (MPA) off west Brittany, France. A return rate of 14.6% associated with long time series of data provided new information on fish ecology (e.g. maximum experienced depth greater than 225 m, temperature range 6.80–21.87°C). Depth and temperature series were used to infer individual migration using an innovative hidden Markov model (HMM) especially developed for seabass geolocation. Reconstructed fish tracks revealed that seabass is a partially migratory species, as individuals exhibited either long-distance migrations towards the Bay of Biscay or the Celtic Sea, or residency behaviour in the Iroise Sea. Fidelity to summer...
Tipo: Text Palavras-chave: Cod; Connectivity; Data Storage Tag (DST); Depth; European sea bass; Dicentrarchus labrax; Geolocation; Partial migration; Spawning site fidelity; Temperature; Vertical movement.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00451/56230/57790.pdf
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Effect of dietary water content on European sea bass (Dicentrarchus labrax) growth and disease resistance ArchiMer
Przybyla, Cyrille; Fievet, Julie; Callier, Myriam; Blancheton, Jean-paul.
The effect of dietary water content on Dicentrarchus labrax growth parameters and resistance to Vibrio anguillarum infection was investigated using commercial pellets with identical energy contents and different moisture levels. The first experiment hypothesis was that moisturizing pelleted ration can have an impact on Dicentrarchus labrax growth performance by the osmoregulation energy cost reduction. In a second time, the experiment explores the effect of water addition in pellets on the fish resistance to a disease. A specific device was built to uniformly moisturize dry pellets to different moisture levels, i.e. 8%, 20%, 40% and 60%. After an acclimation period and a 54-day rearing period, the control fish had grown from 72.7 +/- 17.9 g to 133.3 +/-...
Tipo: Text Palavras-chave: Fish aquaculture; Dietary moisture content; Feed pellets; Fish growth; Immunity; Dicentrarchus labrax; Vibrio anguillarum.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35927/34446.pdf
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Approche du regime alimentaire des juveniles de bars et de limandes en baie de Somme. ArchiMer
Leaute, Jean-pierre.
Stomach contents of juveniles of sea bass (Dicentrarchus labrax ) and common dab (Limanda limanda ) collected during September 1982 from Bay of Somme and the neighbor coast to Penly, were examined. The most part of the diet of young sea bass are the crustaceans, Sedentary polychaetes appear to be the major food item for common dab. For these two young predator species, food preferences tend to change early: cumaceans, then common edible shrimp, for sea bass, Tube worm tentacles, then part of whole individuals are preferred by common dab.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Prey selection; Food preferences; Stomach content; Diets.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/publication-1789.pdf
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Etude cytologique ultrastructurale des anomalies hepatiques du loup, de la daurade, et de l'anguille, induites par une alimentation artificielle ArchiMer
Bac, N; Biagianti, S; Brusle, J.
In the three species Dicentrarchus labrax, Sparus auratus , and Anguilla anguilla an artificial feeding (dry pellets) disturbs liver cells as revealed at ultrastructural level. In hepatocytes, anomalies consist of lipids storage (liposomes) considered as deviation in fatty acids metabolism. Moreover are observed cellular alterations (mitochondrial vacuolization, nuclear pycnosis) related to degenerative process. It is of great interest to understand wether liver modifications are just a reaction to an unbalanced feeding or a true trophic pathology.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Natural populations; Fish culture; Lipids; Fatty acids; Cytology; Ultrastructure; Cells; Diseases; Pellet feeds; Metabolism; Liver; Artificial feeding; Nutrition disorders.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1250.pdf
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Origine, devenir et contrôle de la matière particulaire dans les élevages de poissons marins en système recyclé ArchiMer
Franco, Miguel.
The particulate matter plays an essential role in the functioning of recirculating aquaculture systems (RAS). Rich in organic substances, it provides ideal conditions for the bacterial development, which contribute to its mineralization. The understanding of interactions between the bio-geochemical cycles of carbon and nitrogen in these processes is necessary to define an effective and adapted management of aquaculture facilities. A methodology using natural stable isotopes (13C and 15N) and elemental carbon and nitrogen analysis on particulate matter, allows to identify its sources and to quantify its fluxes. Applying this methodology, we found an enrichment of 13C in dorsal muscle of reared fish and non differences of 13C in fish liver compared to the...
Tipo: Text Palavras-chave: Nitrogen; Carbon; Isotopes; Organic matter; Particulate matter; Dicentrarchus labrax; Seabass; Recirculating systems; Aquaculture; Azote; Carbone; Isotopes; Matière organique; Matière particulaire; Dicentrarchus labrax; Bar; Système de recirculation; Aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/these-322.pdf
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Metal subcellular partitioning determines excretion pathways and sensitivity to cadmium toxicity in two marine fish species. ArchiMer
Le Croizier, Gael; Lacroix, Camille; Artigaud, Sebastien; Le Floch, Stephane; Munaron, Jean-marie; Raffray, Jean; Penicaud, Virginie; Rouget, Marie-laure; Lae, Raymond; Tito De Morais, Luis.
Subcellular cadmium (Cd) partitioning was investigated in the liver of two marine fish species, the European sea bass Dicentrarchus labrax and the Senegalese sole Solea senegalensis, dietary exposed to an environmentally realistic Cd dose for two months followed by a two-month depuration. Cd exposure did not modify Cd cellular partitioning for either species, refuting the spillover hypothesis. Both species contained most of the Cd in the detoxifying fraction but displayed different handling strategies. Cd was largely bound to heat stable proteins (HSP) including metallothioneins (MT) in sea bass while Cd was more linked to metal rich granules (MRG) in sole. Whole liver concentrations and subcellular partitioning were also determined for essential elements....
Tipo: Text Palavras-chave: Sub-cellular fractionation; Dicentrarchus labrax; Solea senegalensis; Essential element distribution; Elimination; Depuration.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00464/57606/59792.pdf
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Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Sea bass, Dicentrarchus labrax ArchiMer
Bado-nilles, Anne; Quentin, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Auffret, Michel; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text Palavras-chave: PaHs; Fish; Contamination; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3427.pdf
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Ontogenèse des fonctions digestives et besoins nutritionnels chez les larves de poissons marins ArchiMer
Cahu, Chantal; Zambonino, Jose-luis.
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first...
Tipo: Text Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf
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Traits biologiques et exploitation du bar commun Dicentrarchus labrax (L.) dans les pêcheries françaises de la Manche et du golfe de Gascogne. ArchiMer
Fritsch, Manon.
Sea bass Dicentrarchus labrax (Linné, 1758) is a fish of major interest in France and in the British Isles. It is the main target species for some anglers and a growing number of professional fishermen. In spite of this, the reproduction of sea bass in natural environment, the identification of its populations, as well as the fleet participating to and the level of its exploitation are poorly known. Thus, in the perspective of a future management of the bass fisheries, this thesis collected required data for the analysis of the sea bass resource in the English Channel and the Bay of Biscay. A first chapter describes to the principal biological and ecological characteristics of Dicentrarchus labrax. The five following chapters deal successively with: (i)...
Tipo: Text Palavras-chave: Fisheries management; Fisheries exploitation; Angling; Tagging; Microsatellite loci; Reproduction; Dicentrarchus labrax; Gestion de stocks; Exploitation; Pêche récréative; Pêcheries; Marquage; Marqueurs microsatellites; Reproduction; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-1088.pdf
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