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Energy, water vapor and carbon fluxes in Andean agroecosystems: conceptualization and methodological standardization Acta Agron. (Palmira)
Castano-Marín,Angela María; Riaño-Herrera,Nestor Miguel; Peña-Quiñones,Andrés Javier; Ramirez-Builes,Víctor Hugo; Valencia-Salazar,Arley; Figueroa-Casas,Apolinar; Góez-Vinasco,Gerardo Antonio.
Abstract This paper presents the conceptualization, methodological adjustment and experimental application of the micrometeorological technique eddy covariance - EC, to measure energy, water vapor and CO2 fluxes in two coffee agroecosystems: the first under full sunlight, and the second under shade, both with equatorial Andean hillslope conditions. With a footprint and fetch calculation, the required distance from the edge of the field in the prevailing wind direction to the EC tower is three times higher under shade than full sun. The shaded agroecosystem reached maximum average carbon fixation rates of 21.26 ± 2.469 μmolCO2.m-2s-1 (α = 0.05) (61% higher than under 100% sunlight) which gives a high carbon sink capacity to the association of coffee plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecosystem coffee; Andean hillslope; Eddy covariance; Energy fluxes; Gas exchange.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000100027
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PHOTOSYNTHETIC RESPONSE TO LOW AND HIGH LIGHT OF CACAO GROWING WITHOUT SHADE IN AN AREA OF LOW EVAPORATIVE DEMAND Acta biol.Colomb.
JAIMEZ,Ramon Eduardo; AMORES PUYUTAXI,Freddy; VASCO,Alfonso; Gastón LOOR,Rey; TARQUI,Omar; QUIJANO,Grisnel; JIMENEZ,Juan Carlos; TEZARA,Wilmer.
ABSTRACT Cacao (Theobroma cacao L.) breeding programmes in Ecuador have focused on obtaining high-yield clones with improved disease resistance. Cacao clones should also have photosynthetic characteristics which support increased productivity. Regarding the weather conditions at the coast of Ecuador, where most of the year there are overcasts and low air evaporative demand, there is the possibility to grow cacao without overhead shade. This study focused on the photosynthetic response at two different photosynthetic photon flux densities (PPFD) of Ecuadorian cacao clones. Seven-year old cacao clones were evaluated: eight clones of Nacional type and two commercial clones (CCN 51 and EET 103), used as controls. All clones showed an increase of 35 % on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cacao yield; Chlorophyll fluorescence; CO2 assimilation rate; Gas exchange.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000100095
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Different physiological responses under drought stress result in different recovery abilities of two tropical woody evergreen species Acta Botanica
Frosi,Gabriella; Harand,Wolfgang; Oliveira,Marciel Teixeira de; Pereira,Silvia; Cabral,Simone Pereira; Montenegro,Abelardo Antônio de Assunção; Santos,Mauro Guida.
ABSTRACT The effects of water deficit on physiological and biochemical variables of young plants of two tropical woody species, Pachira aquatica and Sterculia foetida, and their recovery abilities were measured. Leaf water potential, gas exchange and selected carbon metabolism components were measured in a greenhouse experiment with control, moderate and severe water deficit treatments. Under severe drought stress, gas exchange and chlorophyll fluorescence decreased in both species compared to their respective controls. After rehydration, P. aquatica and S. foetida showed a partial and full recovery of the measured variables, respectively. In addition, a decrease and an increase in photosynthetic pigments were observed for P. aquatica and S. foetida,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyll fluorescence; Climate changes; Gas exchange; Semiarid; Water deficit.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000200153
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Structural and functional leaf traits of two Gochnatia species from distinct growth forms in a sclerophyll forest site in Southeastern Brazil Acta Botanica
Rossatto,Davi Rodrigo; Kolb,Rosana Marta.
Gochnatia is very common in different phytophysiognomies in the Cerrado of São Paulo State, occupying open and closed areas. In this study, we compared the leaf anatomy and some ecophysiological traits of two species of Gochnatia, one a shrub (Gochnatia barrosii Cabrera) and the other a tree (Gochnatia polymorpha (Less.) Cabrera), which both occur in an area of "cerradão" at the Estação Ecológica de Assis, SP. We found qualitative structural differences between the species, with G. barrosii presenting amphistomatic leaves with a uniseriate epidermis and G. polymorpha showing hypostomatic leaves and a multiple epidermis or hypodermis on the adaxial surface. Moreover, the G. barrosii leaves had lower values in tissue thickness (with the exception of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Gas exchange; Leaf anatomy; Stomata.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062012000400014
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Growth and photosynthetic responses during ex vitro acclimatization of Etlingera elatior (Jack) rm smith (torch ginger) Agronomy
Rodrigues, Marcelo; Paiva, Patrícia Duarte de Oliveira; Freitas, Rodrigo Therezan de; Mansur, Thaís de Oliveira Fontes; Paiva, Renato; Barbosa, João Paulo Rodrigues Alves Delfino.
Torch ginger (Etlingera elatior (Jack) RM Smith) can be propagated in vitro, but there is currently little information about the stages of acclimatization and adaptation for torch ginger in a greenhouse. The objective was to study the growth responses, survival and photosynthetic response during the acclimatization of plants maintained on three different substrates (Plantmax forestry®, sand and 1:1 Plantmax forestry® and sand) under three shading conditions (50% red and blue shading nets and control without shading nets). The highest leaf and shoot numbers per plant and survival rate were observed in treatments with the Plantmax forestry® type substrate in the absence of shading. In vitro culture plants behaved similarly or better than rhizome propagated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.00.00.00-4 torch ginger; Growth curve; Gas exchange; Photoautotrophy. 5.01.03.06-7.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19707
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Photosynthetic pigments and gas exchange in castor bean under conditions of above the optimal temperature and high CO2 Agronomy
Silva, Fabiola França; Mendes, Bruna Silva; Rocha, Maria Socorro; Brito Neto, José Félix; Beltrão, Napoleão Macêdo; Sofiatti, Valdinei.
The castor bean plant, a Euphorbiaceae oil seed C3-metabolism rustic and drought-resistant plant, is cultivated in a wide range of environments due to its good adaptive capacity. However, given the current environmental changes, many biochemical and physiological impacts may affect the productivity of important crops, such as castor bean. This work aimed to evaluate the impacts of the castor bean gas exchange in response to high temperature and increased CO2concentration.Our experiment was conducted in a phytotron located at Embrapa Algodão in 2010. We adopted a completely randomized design, with four treatments in a factorial combination of two temperatures (30/20 and 37/30°C) and two CO2 levels (400 and 800 mmol L-1); four replications were performed,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 castor bean; Climate change; Gas exchange; Photosynthesis; Chlorophyllssíntese clorofila 5.01.03.06-7.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19075
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Physiological and agronomical responses of Syrah grapevine under protected cultivation Bragantia
Souza,Claudia Rita de; Mota,Renata Vieira da; Dias,Frederico Alcântara Novelli; Melo,Evaldo Tadeu de; Pimentel,Rodrigo Meirelles de Azevedo; Souza,Laís Cristina de; Regina,Murillo de Albuquerque.
The performance of Syrah grapevine under protected cultivation with different plastic films was evaluated during 2012 and 2013 seasons in South of Minas Gerais State. Agronomical and physiological measurements were done on eight years old grapevines, grafted onto ‘1103 Paulsen’ rootstock cultivated under uncovered conditions, covered with transparent and with diffuse plastic films. Both plastic covers induced the highest shoot growth rate and specific leaf area. The diffuse plastic induced greater differences on leaf area, pruning weight and leaf chlorophyll content as compared to uncovered vines. Grapevines under diffuse plastic also had the lowest rates of photosynthesis, stomatal conductance and transpiration. Leaf starch, glucose and fructose contents...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis vinifera; Plastic cover; Vegetative vigor; Water relations; Gas exchange; Wine grape composition.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000300270
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Changes in phenotypic variability of two tropical woody species due to short and long-term exposure to different irradiances Bragantia
Pereira,Silvia; Frosi,Gabriella; Oliveira,Marciel Teixeira de; Lustosa,Bruno Melo; Arruda,Emília Pereira; Santos,Mauro Guida.
ABSTRACT Studies addressing the physiological and anatomical responses of plants under different light intensities normally are performed in short term. Thus, the present study includes a long term analysis. This study aims to verify whether the phenotypic variability off attributes in two woody tropical specieis, Paquira aquatica and Sterculia foetida, are compatible both under short term exposure to different light availability levels (full-light and half-light local irradiance) and long-term exposure in the same conditions. The study was developed in two phases: phase I (short term) and phase II (long term). The variables measured were referent to CO2 assimilation responses to light intensity, chlorophyll a fluorescence, chlorophyll content,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyll a fluorescence; Gas exchange; Plastic response; Light curve.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000300429
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Ecophysiological leaf traits of native and exotic palm tree species under semi-arid conditions Bragantia
Oliveira,Deborah; Medeiros,Maria; Pereira,Silvia; Oliveira,Marciel; Frosi,Gabriella; Arruda,Emília; Santos,Mauro.
ABSTRACT The main goal of this study was to evaluate the performance of two palm species under semi-arid conditions during the rainy and dry periods: the semi-arid native Syagrus coronata and a native to tropical America, Acrocomia aculeata. The leaf water potential, gas exchange, leaf soluble sugars, starch, free amino acids, total soluble protein content and morphological traits were measured. The highest leaf water potential and CO2 assimilation values in both species were achieved during the rainy period. In response to the low soil moisture content during the dry period, gas exchange decreased 72 and 92% in S. coronata and A. aculeata, respectively, when compared with values from rainy period. As evergreen plants, both species maintained intact leaf...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acrocomia aculeata; Evergreen species; Gas exchange; Syagrus coronata; Water deficit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000200128
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Potassium-modulated physiological performance of mango plants infected by Ceratocystis fimbriata Bragantia
Cacique,Isaias Severino; Bispo,Wilka Messner Silva; Araujo,Leonardo; Aucique-Pérez,Carlos Eduardo; Rios,Jonas Alberto; Silva,Leandro Castro; Rodrigues,Fabrício Ávila.
ABSTRACT Mango wilt, caused by the fungus Ceratocystis fimbriata, is an important disease affecting mango production. In view of the beneficial effects of potassium (K) in other profitable crops and the lack of information about the effect of macronutrients on mango wilt development, the present study aimed to evaluate how mango plants supplied with K respond physiologically when infected by C. fimbriata. Mango plants (» 3 years old) from cultivar Ubá were grown in plastic pots containing 58 mg of K·dm−3 (original K level based on the chemical analysis of the substrate) or in plastic pots with substrate amended with a solution of 0.5 M potassium chloride (KCl) to achieve the rate of 240 mg K·dm−3. Disease symptoms were more pronounced in inoculated plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Gas exchange; Host defense; Mango wilt; Plant nutrition; Vascular disease.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400521
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Photosynthetic response of soybean to twospotted spider mite (Acari: Tetranychydae) injury BABT
Bueno,Adeney de Freitas; Bueno,Regiane Cristina Oliveira de Freitas; Nabity,Paul David; Higley,Leon George; Fernandes,Odair Aparecido.
The twospotted spider mite Tetranychus urticae Koch is a common pest on soybean plants. To clarify plant-arthropod interaction on mite-soybean system, leaf fluorescence, photosynthetic responses to variable carbon dioxide levels, and chlorophyll content were evaluated. Significant photosynthetic rate reduction was observed due to stomatal limitation. Stomatal closure was the major plant physiological response. As a consequence, there was reduction in photosynthetic rates. Surprisingly, plants did not show chlorophyll content reduction associated with photosynthetic impairment. No differences in fluorescence data indicate that T. urticae injury did not impair the function of light harvesting and photoelectron transport. These results showed that T. urticae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tetranychus urticae; Insect-plant relationship; Fluorescence; Gas exchange; Photosynthesis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400005
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Anatomy of pneumatophore of Mauritia vinifera mart BABT
Pereira,Luiz Alfredo Rodrigues; Calbo,Maria Elisa Ribeiro; Ferreira,Claiton Juvenir.
Pneumatophores of Mauritia vinifera Mart. were collected from six month-old plants maintained submerged in fresh water to induce pneumatophore formation. Twenty day-old pneumatophores had a quite prominent root cap. The epidermis was composed of hexagonal cells, tangentially distributed along the cylindric surface of the organ. In transverse section these pneumatophores had a simple epidermis over several layers of sclerified parenchyma, which covered an aerenchyma with large intercellular spaces. The endodermal cells had Casparian strips. The vascular cylinder was polyarch, with a pith and surrounded by a unisseriate pericycle. Anatomically the 4 month-old pneumatophores were similar to the younger ones, except for the absence of the epidermis. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buriti; Mauritia vinifera; Pneumatophores; Gas exchange.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300013
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Intensity and physiological responses to the 6-minute walk test in middle-aged and older adults: a comparison with cardiopulmonary exercise testing BJMBR
Sperandio,E.F.; Arantes,R.L.; Matheus,A.C.; Silva,R.P.; Lauria,V.T.; Romiti,M.; Gagliardi,A.R.T.; Dourado,V.Z..
The 6-minute walk test (6MWT) is a simple field test that is widely used in clinical settings to assess functional exercise capacity. However, studies with healthy subjects are scarce. We hypothesized that the 6MWT might be useful to assess exercise capacity in healthy subjects. The purpose of this study was to evaluate 6MWT intensity in middle-aged and older adults, as well as to develop a simple equation to predict oxygen uptake ( V ˙ O 2) from the 6-min walk distance (6MWD). Eighty-six participants, 40 men and 46 women, 40-74 years of age and with a mean body mass index of 28±6 kg/m2, performed the 6MWT according to American Thoracic Society guidelines. Physiological responses were evaluated during the 6MWT using a K4b2 Cosmed telemetry gas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Exercise; Walking; Gas exchange.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400349
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Water deficit affects photosynthetic induction in Bauhinia forficata Link (Fabaceae) and Esenbeckia leiocarpa Engl. (Rutaceae) growing in understorey and gap conditions Braz. J. Plant Physiol.
Portes,Maria T.; Alves,Thiago H.; Souza,Gustavo M..
In tropical forests, light is considered the most limiting resource for plant growth and reproduction. Besides light, water deficit leads invariably to a decrease in photosynthesis. Thus, despite of the unquestionable role of light in CO2 assimilation (A), it is expected that water deficit affects and limits the light utilization by plants. In this study, we investigated how water deficit influenced the photosynthetic induction of the early successional tree Bauhinia forficata (Fabaceae) and the late successional Esenbeckia leiocarpa (Rutaceae) in the understorey and in the forest gap. Field measurements were carried out in June and August 2006 in plants of approximately two-years-old. In August, the plants were subjected to a period of 45 d without rain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drought; Ecophysiology; Gas exchange; Light utilization; Sunflecks; Tropical forest succession.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400007
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Influence of chromium in Laguncularia racemosa (L). Gaertn f. physiology Braz. J. Plant Physiol.
Rocha,Andréa C.; Canal,Emerson C.; Campostrini,Eliemar; Reis,Fabrício O.; Cuzzuol,Geraldo Rogério F..
Most studies of chromium have been realized in the sediments and in mangrove trees. However, little is known about its effect on growth and metabolism in this vegetation. L. racemosa plants were grown in solution with 20% of ionic strength of Hoagland and Arnon containing 100 mM NaCl and supplemented with 0.00, 0.05 and 0.50 mg L-1 of Cr+3. The growth, the pigments and gas exchange measurements showed no changes in response to doses of chromium. After 30 days the initiation of treatments, plants of L. racemosa had average 0.559 g of total dry weight, 1.34 mg L-1 of total chlorophyll and 7.9 µmol m-2 s-1 of A (CO2 photosynthetic assimilation). The photochemical potential efficiency was affected by the intermediate dose of 0.05 mg L-1 of Cr+3 (Fv/Fm=0.73)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Fluorescence; Gas exchange; Growth; Heavy metal; Mangrove.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000200001
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Limitations to photosynthesis at different temperatures in the leaves of Citrus limon Braz. J. Plant Physiol.
Pimentel,Carlos; Bernacchi,Carl; Long,Steve.
The response of CO2 assimilation rate (A) to the intercellular partial pressure of CO2 (Ci) was measured on intact lemon leaves over a range of temperatures (10 to 40ºC). The A/Ci response shows how change in the leaf temperature alters the activity of ribulose-1,5-bisphosphate (RuBP) carboxylase-oxygenase (Rubisco) and RuBP regeneration via electron transport. The rate of A reached a maximum of 7.9 to 8.9 µmol m-2 s-1 between 25 and 30ºC, while dark respiration (Rd) increased with temperature from 0.4 µmol m-2 s-1 at 10ºC to 1.4 µmol m-2 s-1 at 40ºC. The maximum rates of carboxylation (Vc,max) and the maximum rates of electron transport (Jmax) both increased over this temperature range from 7.5 to 142 µmol m-2 s-1 and from 23.5 to 152 µmol m-2 s-1,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: A/Ci curves; Gas exchange; Lemon.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000200006
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Photosynthetic activity in the rootstock of hybrid peach trees submitted to water restriction and flooding Braz. J. Plant Physiol.
Martinazzo,Emanuela G.; Perboni,Anelise T.; Farias,Márcio E.; Bianchi,Valmor J.; Bacarin,Marcos A..
This study aimed to evaluate the effect of flooding and water restriction (drought) on the photosynthetic activity in plants of the hybrid rootstock 'GxN-9' (Prunus dulcis Mill. × Prunus persica L. Bastsch), grown in a greenhouse. Chlorophyll fluorescence and gas exchange were analyzed in plants of approximately 12 months. The reduction of photosynthetic activity in both the stresses were associated with decreased carboxylation efficiency, but without affecting the internal concentration of carbon dioxide. The JIP-test parameters revealed changes in the energy flux processes demonstrating that in terms of flooding the rootstock GxN-9 the rate of photosynthetic performance significantly decreases, resulting in a considerable decrease in net assimilation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Chlorophyll a fluorescence; Gas exchange; JIP-test; Prunus dulcis Mill. × Prunus persica L. Bastsch.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000300007
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Leaf heliotropism in Styrax camporum Pohl from the Brazilian cerrado: distinct gas exchange and leaf structure, but similar leaf temperature and water relations Braz. J. Plant Physiol.
Habermann,Gustavo; Machado,Silvia R.; Guimarães,Vandeir F.; Rodrigues,João D..
Styrax camporum is a common shrub species from the cerrado regions of São Paulo State, Brazil. Although its leaves do not have a morphologically delimited pulvinus, the apical leaves are oriented parallel to the sun (paraheliotropic leaves), most notably around noon. Horizontal leaves at the base of shoots are oriented perpendicular to the sun's rays (diaheliotropic leaves). The ecophysiological significance of this is not fully understood. We investigated how paraheliotropism influences daily CO2 assimilation (A) and other gas exchange rates, water relations, leaf temperature (Tl), and how these relate to leaf structure as assessed by anatomical leaf descriptions. Paraheliotropic leaves had greater A and stomatal conductance (g s), which led to greater...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian savanna; Ecophysiology; Gas exchange; Leaf movements; Leaf temperature; Photosynthesis; Styracaceae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000100008
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Light as an indicator of ecological succession in brazilwood (Caesalpinia echinata Lam.) Braz. J. Plant Physiol.
Mengarda,Liana H. G.; Souza,Rogério L. F. de; Campostrini,Eliemar; Reis,Fabrício O.; Vendrame,Wagner A.; Cuzzuol,Geraldo R. F..
The ecophysiological behavior of brazilwood (Caesalpinia echinata Lam.) plants was evaluated as affected by light quantity and quality. Nine-month-old plants were cultivated under 0%, 50%, and 80% artificial shading, and natural shading imposed by a closed canopy for a period of 392 days. At the end of that period growth parameters were measured, including relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), specific leaf mass (SLM) and root:shoot ratio (R:S). Higher leaf area, dry mass and RGR were obtained under 50% artificial shade. Under full sunlight (0% shade), growth was satisfactory showing higher R:S and SLM. Growth was inhibited under 80% artificial and natural shading indicating that C. echinata is not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyll fluorescence; Gas exchange; Growth; Light stress; Shade; Tropical tree.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000100007
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Growth and photosynthetic responses of Picea asperata seedlings to enhanced ultraviolet-B and to nitrogen supply Braz. J. Plant Physiol.
Yao,Xiaoqin; Liu,Qing; Han,Chao.
Ultraviolet-B (UV-B) radiation and nitrogen are expected to increase simultaneously with future changes in global climate. In this study, growth and photosynthetic responses of Picea asperata seedlings to enhanced UV-B and to nitrogen supply were studied. The experimental design included two levels of UV-B treatments (ambient UV-B, 11.02 kJ m-2 d-1; enhanced UV-B, 14.33 kJ m-2 d-1) and two nitrogen levels (0; 20 g N m-2) to determine whether nitrogen can alleviate the negative impacts of enhanced UV-B on seedling growth and photosynthesis. Enhanced UV-B significantly inhibited plant growth and impaired net photosynthetic rate, stomatal conductance, transpiration rate, the light-saturated assimilation rate, assimilation capacity, light compensation point,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth; Gas exchange; Nitrogen; Picea asperata; Photosynthesis; UV-B.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000100002
Registros recuperados: 68
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