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Registros recuperados: 59
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Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia Rev. Bras. Zool.
Aguirre-Obando,Oscar A.; Bona,Ana C. Dalla; Duque L.,Jonny E.; Navarro-Silva,Mário A..
Mosquito control prevails as the most efficient method to protect humans from the dengue virus, despite recent efforts to find a vaccine for this disease. We evaluated insecticide resistance and genetic variability in natural populations of Aedes aegypti (Linnaeus, 1762) from Colombia. This is the first Colombian study examining kdr mutations and population structure. Bioassays with larvae of three mosquito populations (Armenia, Calarcá and Montenegro) were performed according to the World Health Organization (WHO) guidelines, using Temephos. For the analysis of the Val1016Ile mutation and genetic diversity, we sampled recently-emerged adults from four mosquito populations (Armenia, Calarcá, Montenegro and Barcelona). Following the WHO protocol, bioassays...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioassays; Gene flow; Knockdown resistance; Mitochondrial DNA; ND4 gene.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000100014
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ความหลากหลายทางพันธุกรรมและโครงสร้างประชากรของข้าววัชพืชในประเทศไทย Thai Agricultural
Anupong Wongtamee; Chanya Maneechote; Benjavan Rerkkasem; Sansanee Jamjod.
Weedy rice (Oryza sativa f. spontanea) is one of most serious weeds in direct-seeded rice production. High genetic diversity and adaptability of weedy rice made it very difficult to control in the rice field. This study aim to assess genetic diversity and population structure of weedy rice populations from Lower North, Northeast and Central of Thailand in wet of 2009 using twelve microsatellite markers. Genetic diversity and population structure of weedy rice were determined and compared with seven common wild rice populations and seven check varieties commonly grown in these regions. Weedy rice populations in this present study were hybrid between common wild rice and crop rice. The population structure of them was structured into 2 clusters according to...
Tipo: Collection Palavras-chave: Weedy rice; Genetic diversity; Population structure; Gene flow; ข้าววัชพืช; ความหลากหลายทางพันธุกรรม; โครงสร้างประชากร; การแลกเปลี่ยนยีน; ประเทศไทย.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5333
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Geographic pattern of genetic diversity in natural populations of Rosewood (Aniba rosaeodora), in the Central Amazonia Acta Amazonica
Santos,Ronaldo Pereira; Ângelo,Paula Cristina da Silva; Sampaio,Paulo de Tarso Barbosa; Quisen,Regina Caetano; Leite,Ângela Maria Conte; Oliveira,Cristiane Lopes de.
Rosewood (Aniba rosaeodora Ducke, Lauraceae) is an Amazonian evergreen tree and a source of the purest linalool, the main component of its essential oil, which is very valuable in the international perfumery market. After decades of over-exploitation it is currently considered as threatened. We evaluated the genetic diversity and its distribution in four populations in Central Amazonia. Thirty-five reliable RAPD markers were generated, of which 32 were polymorphic (91.4%). Variation was higher within the populations (76.5%; p < 0.0001) and geographic distribution contributed to population differentiation (23.4%; p < 0.0001). The Amazon River had a small influence on gene flow (3.3%; p < 0.0001), but we identified evidence of gene flow across the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Linalool; Gene flow; Amazon Rainforest.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000300010
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Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers Acta Amazonica
GUIZADO,Stalin Juan Vasquez; NADEEM,Muhammad Azhar; ALI,Fawad; BARUT,Muzaffer; HABYARIMANA,Ephrem; GÓMEZ,Tedi Pacheco; SANTILLAN,Jhon Antoni Vargas; CANALES,Esperanza Torres; GÓMEZ,Juan Carlos Castro; CHUNG,Gyuhwa; BALOCH,Faheem Shehzad.
ABSTRACT Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aniba rosaeodora; Endangered species; Gene flow; Germplasm; Molecular characterization.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300204
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Genetic diversity in rosewood saplings (Aniba rosaeodora ducke, Lauraceae): an ecological approach Acta Amazonica
Santos,Ronaldo Pereira; Spironello,Wilson Roberto; Sampaio,Paulo de Tarso Barbosa.
This article takes an ecological approach to the genetic diversity of Rosewood (Aniba rosaeodora Ducke) in a central Amazonian terra firme forest north of Manaus. Planted Rosewood setting, under partial shaded canopy, were assessed in terms of fruiting production, frugivory, and seed dispersal. Using RAPD molecular analysis procedures, the influence of the spatial distribution of adult trees on the genetic diversity (polymorphism) of saplings was assessed with genetic samples from 34 reproductive trees and 60 saplings. The density and distribution patterns the reproductive trees did not modify the sapling"s diversity (1.86%, AMOVA). Two types of adult tree dispersion were identified; i) clumped and ii) more widely dispersed. Polymorphism (77.5%) and gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian tree; RAPD; Seed dispersal; Gene flow; Genetic diversity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400014
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Pollen and seed dispersal of Brazil nut trees in the southwestern Brazilian Amazon Acta Amazonica
MARTINS,Karina; SANTOS,Raifanny da Silva Oliveira dos; CAMPOS,Tatiana de; WADT,Lúcia Helena de Oliveira.
ABSTRACT Pollen and seed dispersal patterns greatly influence the spatial distribution of plant genetic diversity. Microsatellite-based parentage analysis provides accurate estimates of contemporary gene dispersal. Although most tropical trees have been shown to exhibit widespread pollen dispersal, few studies have estimated contemporary gene dispersal after seedling establishment. Bertholletia excelsa (Lecythidaceae) is pollinated by large-bodied bees, while previous seed-tracking experiments suggest their seeds are mainly dispersed across very short distances by scatter-hoarding rodents, who primarily act as seed predators. Here we used parentage analysis to provide contemporary estimates of pollen and seed dispersal in B. excelsa recruits. We examined...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bertholletia excelsa; Gene flow; Genetic diversity; Pollen flow; Seed flow.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000300217
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SYSTEMATIC CONSIDERATIONS ON TWO SPECIES OF NECTARIVOROUS BATS (Anoura caudifer AND A. geoffroyi) BASED ON BARCODING SEQUENCES Acta biol.Colomb.
VARGAS-ARBOLEDA,Andrés; CUADRADO-RÍOS,Sebastian; MANTILLA-MELUK,Hugo.
ABSTRACT As a product of recent revisions of the nectivore bat genus Anoura (Glossophaginae: Anourina), new species have been described, and several taxa have been elevated to the specific level in the so-called Anoura caudifer and A. geoffroyi species complexes in the last four decades. The genus has a wide distribution across regions of high ecological complexity, including geographic features, such as the Andes and the Isthmus of Panamá, previously identified as natural barriers that restrict gene flow in other phyllostomid bat genera. Up to date, no analyses have been conducted to investigate the contribution of biogeographic factors on the genetic divergence of the genus. In this work, we analyze barcoding DNA sequences (cytochrome oxidase subunit I,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecuadorian pacific; Gene flow; Genetic diversity; Tailless bat.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200194
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Genetic diversity in Calibrachoa pygmaea (Solanaceae): A hawkmoth-pollinated nightshade from the Pampas Acta Botanica
Mäder,Geraldo; Backes,Alice; Reck-Kortmann,Maikel; Freitas,Loreta B..
ABSTRACT Calibrachoa pygmaea is a unique species of Calibrachoa, especially concerning its flower morphology and the environment where it occurs. The species is self-incompatible and is narrowly distributed in wet and flooded fields of the Pampas region. We characterize the genetic diversity of the species based on traditional plastid markers and newly developed nuclear microsatellites to identify drivers that guide its evolution. Our results identified markers that are informative and useful for studying the population structure of C. pygmaea, as well as that of other species of Calibrachoa. Both marker sets were congruent in developing conclusions regarding the evolutionary scenario of C. pygmaea, and revealed that the genetic variability and population...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cross-amplification; Gene flow; Genetic diversity; Plastid haplotypes; Population genetics; SSR markers; Wild petunias.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400664
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Genetic structure of Dicksonia sellowiana Hook. (Dicksoniaceae) reveals clinal distribution along the latitudinal gradient of the Atlantic Forest Acta Botanica
Fagundes,Bruna Saviatto; Poersch,Maria Augusta; Santos,Jaqueline dos; Gaglioti,André Luiz; Labiak,Paulo Henrique; Muschner,Valeria Cunha.
ABSTRACT Dicksonia sellowiana is the only species of the genus occurring in Brazil. Its distribution is restricted to humid areas of the Atlantic Forest biome. The distribution pattern of biodiversity in this biome is known to have been influenced by historical and environmental factors, although the pattern for ferns remains unknown. This study is first to describe the genetic structure of D. sellowiana along the latitudinal gradient of the Atlantic Forest biome. We use microsatellite markers to estimate genetic diversity and structure for 267 individuals representing 14 populations of D. sellowiana from the Atlantic Forest. The results (Ho, He, Fst, Fis, distance genetic) support the hypothesis of a pattern of biodiversity discontinuity. We found greater...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Neotropics; Gene flow; Genetic diversity; Microsatellites; Tree fern.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400712
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Allelic diversity in populations of Solanum lycocarpum A. St.-Hil (Solanaceae) in a protected area and a disturbed environment Acta Botanica
Moura,Tânia Maria de; Sebbenn,Alexandre Magno; Martins,Karina; Moreno,Maria Andreia; Oliveira,Giancarlo Conde Xavier; Chaves,Lázaro José; Kageyama,Paulo Yoshio.
This study aimed to compare the genetic diversity of populations of Solanum lycocarpum A.St.-Hil between natural and human disturbed environments, with the assumption that protected areas have greater genetic diversity than disturbed areas. For this study, two populations were sampled in Goiás State, Brazil. One was located in a conservation unit, Serra de Caldas Novas State Park, in the Caldas Novas municipality. The other was located in a pasture area in the municipality of Morrinhos. The two populations are 41 km apart. We sampled 60 individuals from each population, which were genotyped with five microsatellite loci (SSR). The highest number of alleles was recorded in the population of the conservation unit, where we found 11 exclusive and five rare...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Gene flow; Conservation genetics; Genetic diversity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000400023
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Evidence of genetic differentiation and karyotype evolution of the sedges Cyperus ligularis L. and C. odoratus L. (Cyperaceae) Acta Botanica
Cruz,Geyner Alves dos Santos; Vasconcelos,Santelmo; Pinangé,Diego Sotero de Barros; Gonçalves-Oliveira,Rodrigo Cesar; Alves,Marccus Vinícius; Torres,Rodrigo Augusto; Benko-Iseppon,Ana Maria.
ABSTRACT The taxonomy of Cyperaceae is complex, with genera like Cyperus harboring species complexes. We analyzed the genetic similarity between Cyperus ligularis L. and C. odoratus L. based on DNA fingerprinting and cytogenetics. Significative genetic differentiation (G ST = 0.363) and low gene flow (N m = 0.877) indicated a clear genetic distinction between the two species. Moreover, the clustering analysis showed two distinct genetic groups, suggesting a lack of evidence for hybridization. The phenogram revealed two different lineages, and although all individuals of C. odoratus were collected from plots close to each other, they possessed greater genetic diversity than that observed among individuals of C. ligularis, which were sampled over a wider...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agmatoploidy; DNA amplification fingerprinting; Gene flow; Genetic differentiation; Hybridization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200264
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Population genetic structure of the sheath blight pathogen Rhizoctonia solani AG-1 IA from rice fields in China, Japan and the Philippines Agronomy
Cumagun, Christian Joseph Rili; McDonald, Bruce Alan; Arakawa, Masao; Castroagudín, Vanina Lilian; Sebbenn, Alexandre Magno; Ceresini, Paulo Cezar.
Sheath blight, caused by the fungal pathogen Rhizoctonia solani AG-1 IA is one of the most important rice diseases worldwide. The objetives of this study was to determine the predominant reproductive system and the genetic structure of 18 rice-infecting populations of R. solani sampled from China, Japan and the Philippines, the most important rice production countries in Asia. Knowledge about the population genetic structure of the pathogen in Asia is useful in identifying sources of infection and formulating sustainable management strategies for rice sheath blight. From a total of 717 isolates, 423 unique multilocus genotypes were detected based on nine microsatellite loci. The three country populations of R. solani AG-1 IA exhibited a mixed reproductive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thanatephorus cucumeris; Microsatellite genotyping; Reproductive system; Gene flow; Migration..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42457
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Genetic diversity of the sunflower caterpillar (Chlosyne lacinia saundersii Doubleday and Hewitson) (Lepidoptera: Nymphalidae) populations determined by molecular RAPD markers Anais da ABC (AABC)
Cunha,Fabiane; Gómez,Daniel R. Sosa; Silva,Jose J. da; Alexandre,Talita M.; Moscardi,Flávio.
Chlosyne lacinia saundersii is one of the most important pests of sunflower and it is the main target of insecticides applications. Larvae were collected in Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) and Vilhena (RO). Genomic DNA was extracted and amplified with ten-mer primers, which produced 101 loci. The size of the RAPD amplicons ranged from 180 to 2564 bp. Polymorphism among populations ranged from 31% to 67%, with the highest polymorphisms of 57% and 67% being detected in Uberaba and Vilhena populations, respectively. Populations with the highest similarity determined with Dice coefficient were from Ribeirão Preto and Barreiras, while insects from Londrina showed the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amova; Gene flow; Molecular marker; Sunflower pest.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400029
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Honey Bees of Santa Catarina, Brazil, have only African mitochondrial DNA Arq. Bras. Med. Vet. Zootec.
Lopes-da-Silva,M.; Dalbó,M.A.; Schfaschek,T.P.; Arioli,C.J.; Stefaniak,L.C..
As diferenças entre a subspécie de abelha africana (Apis mellifera scutellata) e as subspécies europeias (Apis mellifera mellifera e Apis mellifera ligustica) nos quesitos comportamento higiênico e agressividade são marcantes. Diferenças acentuadas no comportamento higiênico entre colônias de abelhas foram relatadas em Santa Catarina, Brasil. Suspeitou-se que essas diferenças fossem devidas a um possível fluxo gênico entre as abelhas africanizadas brasileiras e as abelhas europeias da Argentina. Amostras de abelhas de 30 localidades de Santa Catarina foram analisadas por meio do uso de um marcador PCR-RFLP do DNA mitocondrial específico para identificação da origem da linhagem maternal. Os resultados indicaram ausência de linhagem materna de origem...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hymenoptera; Gene flow; Maternal lineage.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200039
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The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae) Biol. Res.
Moresco,Rafaela M; Maniglia,Thiago C; De Oliveira,Classius; Margarido,Vladimir P.
The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the...
Tipo: Journal article Palavras-chave: ESU; Gene flow; Genetic structure; Molecular marker.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100008
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Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences Biota Neotropica
Borba,Rafael Splendore de; Silva,Edson Lourenço da; Ponzetto,Josi Margarete; Pozzobon,Allan Pierre Bonetti; Centofante,Liano; Alves,Anderson Luis; Parise-Maltempi,Patrícia Pasquali.
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flood pulse; Genetic diversity; Gene flow; Haplotypes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000100004
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Frequency of CCR5delta32 in Brazilian populations BJMBR
Vargas,A.E.; Marrero,A.R.; Salzano,F.M.; Bortolini,M.C.; Chies,J.A.B..
A sample of 103 randomly chosen healthy individuals from Alegrete, RS, Brazil, was tested for the CCR5delta32 allele, which is known to influence susceptibility to HIV-1 infection. The CCR5delta32 allele was identified by PCR amplification using specific primers flanking the region of deletion, followed by electrophoresis on a 3% agarose gel. The data obtained were compared to those reported for other populations and interpreted in terms of Brazilian history. The individuals studied came from a highly admixed population. Most of them were identified as white (N = 59), while blacks and browns (mulattoes) were N = 13 and N = 31, respectively. The observed frequencies, considering the white, black and brown samples (6.8, 3.8, and 6.4%, respectively), suggest...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CCR5; Chemokine receptors; Brazilian population; Gene flow.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300002
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Genetic Variability of the Tomato Leaf Miner (Tuta absoluta Meirick; Lepidoptera: Gelechiidae), in Tunisia, Inferred from RAPD-PCR Chilean J. Agric. Res.
Bettaibi,Asma; Mezghani-Khemakhem,Maha; Bouktila,Dhia; Makni,Hanem; Makni,Mohamed.
The tomato leaf miner Tuta absoluta Meyrick has invaded tomato (Solanum lycopersicum L.) crop in Tunisia since 2008 and is representing today a major threat to the production of this crop. In this study, we used the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology to assess the genetic variability within and among seven populations of T. absoluta, collected on tomato from different regions in Tunisia. Using five RAPD-PCR primers and 108 individuals, 140 polymorphic fragments were recorded. From 335 different RAPD phenotypes generated, 71 were redundant and 264 unique to a specific population. The genetic structure of T. absoluta was investigated using analysis of molecular variance (AMOVA), genetic distances (Fst) and...
Tipo: Journal article Palavras-chave: Invasive species; Molecular markers; Insect populations; Gene flow.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000200008
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An update on genetically modified crops Ciencia e Investigación Agraria
Schwember,Andrés R.
A. R. Schwember. 2008. An update on genetically modified crops. Cien. Inv. Agr. 35(3):231-250. Genetically modified (GM) crops were introduced in the mid 1990s and two principal transgenic technologies currently domínate the market, herbicide-tolerant (HT) crops and insect-resistant crops (Bacillus thuringiensis (Bt) crops). HT crops have simplified weed management practices, reduced crop production costs, and have had positive effects on the environment. However, there are concerns about the potential development of weeds resistant to glyphosate, the main herbicide employed with HT crops. A second major worry associated with the use of HT crops is the potential introgression of genes from GM crops into wild relatives (i.e. gene flow) and its potential...
Tipo: Journal article Palavras-chave: Bt crops; Gene flow; Genetically modified crops; Glyphosate; Golden Rice; Herbicide-tolerant crops; Transgenic crops.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000300001
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