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| Registros recuperados: 89 | |
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| Escobar,A; Oliva,Yuleivys; Abeledo,María Antonia; Vega,E; Cedeño,V. |
| Se desarrolló un procedimiento por Cromatografía Líquida de Alta Resolución (HPLC) para determinar clortetraciclina en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con una solución de acetona ácida. El extracto se analizó por HPLC con una columna de fase reversa C18 y un detector UV (340 nm) con una mezcla de dimetilformamida al 27% como fase móvil. Los resultados de la validación mostraron una linealidad entre 50-1000 µg/mL que responde a una ecuación general de Y=0.23X-2.62 con un coeficiente de regresión de 0.996 (p<0.05) y una variación del intervalo de confianza de la pendiente entre 0.23-0.24. El límite de detección y el de cuantificación fue de 33µg/g y 50 µg/g... |
| Tipo: Journal article |
Palavras-chave: Clortetraciclina; HPLC; Pienso. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200009 |
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| Escobar,A; Abeledo,María Antonia; Vega,E; Cedeño,V. |
| Un procedimiento se desarrolló por cromatografía líquida de alta resolución (HPLC) para determinar Aivlosin en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con acetonitrilo y se concentró cinco veces cuando fue inferior a 20 ppm. El extracto se analizó por HPLC con una columna de fase reversa C18, un detector UV (280 nm) y una mezcla de acetonitrilo: acetato de amonio 0.15M: ácido acético en una proporción de 45:45:10 v/v/v, como fase móvil. Los resultados de la validación mostraron una linealidad entre 5-80 µg/ml, que responde a una ecuación general de Y=-2.60+1.72X con un coeficiente de regresión de 0.999(p<0.05) y una variación del intervalo de confianza de la pendiente entre... |
| Tipo: Journal article |
Palavras-chave: Aivlosin; HPLC; Piensos. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200008 |
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| Escobar,A; Faure,R; Sosa,Dayana; Betancourt,A; Hernández,Damaiky. |
| Se desarrolló una metodología para la determinación de oxitetraciclina en músculo de camarón por cromatografía líquida de alta resolución empleando columna de fase reversa y detección UV a 340 nm. La extracción se desarrolló con una solución Mcllvaine-EDTA y los extractos fueron purificados empleando columnas de extracción fase sólida (C18-500mg-6mL). El método presentó un recobrado entre 78.2±9.9 % con una precisión menor del 10% en las concentraciones de 200 y 400 ng/g, la linealidad mostró un coeficiente de correlación mayor de 0.99 y el límite de detección fue 105 ng/g, Se estableció el periodo de latencia de la oxitetraciclina en músculo de camarones (Litopenaeus vannamei) entre 14-20 días cuando se suministra el alimento medicamentado a una dosis de... |
| Tipo: Journal article |
Palavras-chave: Oxitetraciclina; HPLC; Camarón; Periodo de latencia. |
| Ano: 2010 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000200005 |
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| Pakinee Akkaravessapong; Suganya Wongpornchai; Sunanta Wongpiyachon; Kitsada Pitija; Angsutorn Wasusun; Panawan Suttiarporn. |
| Rice contains several kinds of bio-functional components. In this study, γ-aminobutyric acid (GABA) and Tocopherol, or Vitamin E were identified and their relationships to antioxidant activity using DPPH assay of both brown and pre germinated Thai rice varieties were studied. GABA are one of the two inhibitory neurotransmitters which make the neuron resistant to stimulation by excitatory neurotransmitters and inhibitor of some cancer cells activity whereas Tocopherol, or Vitamin E, which acts as antioxidant and improve blood cholesterol. Identification of GABA and Tocopherol in both brown and pre-germinated brown rice grown from different locations were conducted in eleven varieties, namely: Khao Dawk Mali 105, PTT1, CN1, RD31, Sang yod, RD6, Hom... |
| Tipo: PhysicalObject |
Palavras-chave: Antioxidant activity; Rice varieties; Tocopherol; DPPH assay; Brown rice; Pre-germinated brown rice; GABA; Gamma aminobutyric acid; Vitamin e; Nutritive value; Chemical composition; High performance liquid chromatography; HPLC; ฤทธิ์ต้านอนุมูลอิสระ; กาบา; โทโคฟีรอล; พันธุ์ข้าว; ข้าวกล้อง; ข้าวกล้องงอก; วิตามินอี; โครงสร้างทางเคมี; การวิเคราะห์องค์ประกอบทางเคมี; คุณค่าทางโภชนาการ. |
| Ano: 2013 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5713 |
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| OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda. |
| In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids. |
| Ano: 2015 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017 |
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| Guedes, Mayara Neves Santos; Pio, Rafael; Maro, Luana Aparecida Castilho; Lage, Fabíola Fonseca; Abreu, Celeste Maria Patto de; Saczk, Adelir Aparecida. |
| Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.03.00-8 total phenols; Free radicals; HPLC; Rubus spp. Fitotecnia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/28413 |
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| BUENO,RAFAEL D.; BORGES,LEANDRO L.; GOD,PEDRO I.V. GOOD; PIOVESAN,NEWTON D.; TEIXEIRA,ARLINDO I.; CRUZ,COSME DAMIÃO; BARROS,EVERALDO G. DE. |
| ABSTRACT Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soybean; Carbohydrates; Anti-nutritional factors; Quantitative analysis; HPLC; NIR spectroscopy. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100205 |
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| EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN. |
| Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621 |
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| RESENDE,FERNANDA B.S.; LIRA,LARISSA Q. DE; GRILO,EVELLYN C.; LIMA,MAYARA S.R.; DIMENSTEIN,ROBERTO. |
| The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colostrum; Gestational diabetes; HPLC; Hypovitaminosis A; Postpartum. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100463 |
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| Leite,R.M.H.; Leite,R.C.; Lima,J.A.; Fóscolo,C.B.; Mota,P.M.P.C.; Lobato,F.C.F.; Lage,A.P.. |
| The high pressure liquid chromatography (HPLC) was used for the identification of isoniazid (isonicotinic acid hydrazide) in the milk of cattle treated with a dose of 25 mg/kg/day in alternated days. The effect of milk pasteurization on the isoniazid residue concentration was also studied. The drug excretion presented a cyclic variation, with higher levels in the first day after administration (aa), a mean of 1104.48µg/l, and a decrease two days aa, with a mean of 104.12µg/l. Four days after the last administration of the drug it was not possible to identify residues of isoniazid in the milk of treated animals. Body weight and milk yield influenced the amount of the excreted drug, and pasteurization decreased (mean 47.07%) the concentration of isoniazid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Tuberculosis; Isoniazid; Milk; HPLC. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600018 |
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| Villanoeva,C.N.B.C.; Andrade,E.H.P.; Baffa Junior,J.C.; Souza,M.R.; Cerqueira,M.M.O.P.; Fonseca,L.M.; Penna,C.F.A.M.; Leite,M.O.. |
| Caseinomacropeptide (CMP) index is a method used to detect adulteration of milk by addition of cheese whey, since CMP is a glycopeptide characteristic produced during cheesemaking, and soluble in the whey phase. The objective of this work was to evaluate the caseinomacropeptide index of UHT milk stored under different temperatures. Six batches of recently processed UHT milk were collected and stored under three temperatures (21ºC, 6ºC, and -12ºC) and analyzed by HPLC in the day of the milk collection (day 0) and at 30, 60, 90, and 120 days of storage. The experiment was run as a randomized block design with a 3x5 factorial arrangement, and the Student-Newman-Keuls (SNK) method was used as the post-hoc test (p = 0.05). There was a progressive increase of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UHT whole milk; Caseinomacropeptide; HPLC. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100039 |
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| Andrade,E.H.P.; Souza,M.R.; Fonseca,L.M.; Penna,C.F.A.M.; Cerqueira,M.M.O.P.; Roza,T.; Seridan,B.; Resende,M.F.S.; Pinto,F.A.; Villanoeva,C.N.B.C.; Leite,M.O.. |
| Cheese whey level and caseinomacropeptide (CMP) index of fermented milk beverages added with four levels of cheese whey (0, 10, 20, and 40%) and stored at 8-10oC for 0, 7, 14 and 21 days were determined by high performance liquid chromatography-gel filtration (HPLC-GF). Additionally, the interference of the starter culture and the storage time on the detection of cheese whey and CMP were investigated. Refrigerated storage up to 21 days did not affect (P>0.05) cheese whey and CMP amounts in milk (0% of cheese whey) and in fermented milk beverages added with 10 and 20% of cheese whey (P>0.05). However, cheese whey and CMP amounts were higher than expected (P<0.05) in fermented milk beverages added with 40% of cheese whey and stored for 21 days. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fermented milk beverages; Cheese whey; CMP; HPLC. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300959 |
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| Cremonini,D.N.; Ranzani,J.J.T.; Cunha,M.L.R.S.; Tonin,F.G.; Brandão,C.V.S.; Messadeq,Y.. |
| Avaliou-se a concentração de ofloxacina liberada por uma lente de contato de membrana de celulose biossintética, para tratamento de ceratite bacteriana experimental em cães, pela inoculação de Staphylococcus aureus intraestromal. Comparou-se o tratamento com a lente de contato biossintética impregnada com ofloxacina à terapia tópica convencional. Realizou-se avaliação microbiológica e dosagem de ofloxacina no humor aquoso por meio do método de cromatografia líquida de alto rendimento (HPLC). Houve diferença estatística na contagem de colônias bacterianas entre os olhos com ceratite e os demais grupos, no primeiro dia de coleta. O biomaterial, impregnado com ofloxacina, promoveu liberação gradual durante o período de avaliação, aos três e sete dias; no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Ceratite bacteriana; Membrana biossintética; Ofloxacina; HPLC. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400004 |
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| Lasmar,M.M.; Leite,M.O.; Fonseca,L.M.; Souza,M.R.; Cerqueira,M.M.O.P.; Penna,C.F.A.M.; Couto,C.N.B.; Ferreira,J.M.. |
| High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Raw milk; Bronopol; Cheese whey detection; HPLC. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600037 |
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| Dantas,A.C.S.; Freire,D.P.; Souza,G.R.; Almeida,J.R.G.S.; Rolim,L.A.; Castro,R.N.; Horta,M.C.. |
| ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tick control; Acaricide; Plant extract; Mulberry; HPLC. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300523 |
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| Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan. |
| Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPLC; Salicylic acid; UV-B. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006 |
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| Registros recuperados: 89 | |
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