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Zearalenone adsorption capacity of lactic acid bacteria isolated from pigs BJM
Vega,María F.; Dieguez,Susana N.; Riccio,Belén; Aranguren,Sandra; Giordano,Antonio; Denzoin,Laura; Soraci,Alejandro L.; Tapia,María O.; Ross,Romina; Apás,Ana; González,Silvia N..
ABSTRACT The ability to adsorb zearalenone by five strain of lactic acid bacteria was evaluated: four strains of Lactobacillus spp. isolated from pig rectal swabs and one commercial strain (Lactobacillus rhamnosus). Several factors affecting the adsorption capacity were evaluated in order to improve the adsorption of the mycotoxin by bacteria. The stability of the zearalenone-bacteria complex was analyzed. In every case, bacterial adsorption capacity was higher than 40.0%. The strain showing the highest adsorption (68.2%) was selected for the following steps of this research. The adsorption percentages obtained after processing 6.5 and 7.5 mL MRS broth were 57.40% + 3.53 and 64.46% + 0.76, respectively. The stability of zearalenone-bacteria complex was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zearalenone; Adsorption; Lactic acid bacteria; Lactobacillus; Pigs.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400715
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Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry BJM
Rocha,Ticiana Silva; Baptista,Ana Angelita Sampaio; Donato,Tais Cremasco; Milbradt,Elisane Lenita; Okamoto,Adriano Sakai; Andreatti Filho,Raphael Lucio.
In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Identification; Intestinal adhesion.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040
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Fermentation process for production of apple-based kefir vinegar: microbiological, chemical and sensory analysis BJM
Viana,Roberta Oliveira; Magalhães-Guedes,Karina Teixeira; Braga Jr.,Roberto Alves; Dias,Disney Ribeiro; Schwan,Rosane Freitas.
Abstract The aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with Biospeckle Laser. Brazilian kefir grains were inoculated in apple must for vinegar production. In this study, the microbial community present in kefir, and correspondent vinegar, was investigated using Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. Saccharomyces cerevisiae, Lactobacillus paracasei, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii were the microbial species identified. S. cerevisiae, L. plantarum, A. pasteurianus and A. syzygii were found in smaller quantities at the beginning of the alcoholic fermentation, but were found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Saccharomyces; Lactobacillus; Apple; MALDI-TOF; Biospeckle Laser; Vinegar.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300592
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Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee BJM
Tajabadi,Naser; Mardan,Makhdzir; Saari,Nazamid; Mustafa,Shuhaimi; Bahreini,Rasoul; Manap,Mohd Yazid Abdul.
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Honey bee; Honey stomach; Lactobacillus; Probiotic; 16S rDNA gene sequences.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300008
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Protection by Lactobacillus acidophilus UFV-H2B20 against experimental oral infection with Salmonella enterica subsp. enterica Ser. Typhimurium in gnotobiotic and conventional mice BJM
Moura,Lilian Nobre; Neumann,Elizabeth; Vieira,Leda Quercia; Nicoli,Jacques R..
The ability of Lactobacillus acidophilus UFV-H2B20 to antagonize Salmonella enterica subsp. enterica ser. Typhimurium and to reduce the pathological consequences for the host was determining using conventional and gnotobiotic animals. Conventional NIH mice received daily by gavage a 0.1 ml suspension containing about 10(8) cfu L. acidophilus UFV-H2B20 and germfree animals received a single 0.1 ml dose. The gnotobiotic and conventional groups were infected orally with 10² and 10(5) cfu of S. Typhimurium, respectively, 7 days after the beginning of treatment. Control groups were treated with sterile saline instead of Lactobacillus. Survival data showed a protective effect against the pathogenic bacteria in both conventional and gnotobiotic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Salmonella; Probiotic; Gnotobiotic mice.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100015
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Partial characterization of ribosomal operons of Lactobacillus delbrueckii UFV H2b20 BJM
Magalhães,Juliana Teixeira de; Floresta,Fernanda; Moraes,Célia Alencar de.
Ribosomal operons are great tools for microbe community characterization and for microorganisms relationship study, particularly in the case of the acid lactic bacteria. The ribosomal operon of the probiotic strain Lactobacillus delbrueckii UFV H2b20 was partially characterized. A genomic library of this strain was constructed and the clones with partial ribosomal operon were sub-cloned using the shot-gun method for subsequent sequencing with the forward primer. The sequence analysis revealed that the 3' end of the rDNA 16S was following by the short spacer region 1 (16S-23S) and that the 3' end of the rDNA 23S was following by the short spacer region 2 (23S-5S), which preceded the rDNA 5S. In the flanking region of the rDNA 5S gene of this operon rrn, a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribosomal operons; Lactobacillus; TRNA; Acid lactic bacteria.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200014
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Brazilian kefir: structure, microbial communities and chemical composition BJM
Magalhães,Karina Teixeira; Pereira,Gilberto Vinícius de Melo; Campos,Cássia Roberta; Dragone,Giuliano; Schwan,Rosane Freitas.
Microbial ecology and chemical composition of Brazilian kefir beverage was performed. The microorganisms associated with Brazilian kefir were investigated using a combination of phenotypic and genotypic methods. A total of 359 microbial isolates were identified. Lactic acid bacteria (60.5%) were the major isolated group identified, followed by yeasts (30.6%) and acetic acid bacteria (8.9%). Lactobacillus paracasei (89 isolates), Lactobacillus parabuchneri (41 isolates), Lactobacillus casei (32 isolates), Lactobacillus kefiri (31 isolates), Lactococcus lactis (24 isolates), Acetobacter lovaniensis (32 isolates), Kluyveromyces lactis (31 isolates), Kazachstania aerobia (23 isolates), Saccharomyces cerevisiae (41 isolates) and Lachancea meyersii (15 isolates)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Saccharomyces; Fermented beverage; Brazilian kefir grains.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200034
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Probiotic features of two oral Lactobacillus isolates BJM
Zavisic,Gordana; Petricevic,Sasa; Radulovic,Zeljka; Begovic,Jelena; Golic,Natasa; Topisirovic,Ljubisa; Strahinic,Ivana.
In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Antimicrobial activity; Hipolypemic effect.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100050
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Characterization of Lactobacillus from Algerian goat's milk based on phenotypic, 16S rDNA sequencing and their technological properties BJM
Marroki,Ahmed; Zúñiga,Manuel; Kihal,Mabrouk; Pérez-Martínez,Gaspar.
Nineteen strains of Lactobacillus isolated from goat's milk from farms in north-west of Algeria were characterized. Isolates were identified by phenotypic, physiological and genotypic methods and some of their important technological properties were studied. Phenotypic characterization was carried out by studying physiological, morphological characteristics and carbohydrate fermentation patterns using API 50 CHL system. Isolates were also characterized by partial 16S rDNA sequencing. Results obtained with phenotypic methods were correlated with the genotypic characterization and 13 isolates were identified as L. plantarum, two isolates as L. rhamnosus and one isolate as L. fermentum. Three isolates identified as L. plantarum by phenotypic characterization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactic acid bacteria; Lactobacillus; Identification; Goat's milk; Technological properties; Algeria.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100020
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Influence of fructooligosaccharides on the fermentation profile and viable counts in a symbiotic low fat milk BJM
Oliveira,Ricardo P.S.; Casazza,Alessandro A.; Aliakbarian,Bahar; Perego,Patrizia; Converti,Attilio; Oliveira,Maricê N..
This study evaluated the effects of prebiotics on fermentation profile and growth of Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, and Bifidobacterium lactis in co-cultures with Streptococcus thermophilus. Acidification rate and viability were positively influenced by the co-culture with B. lactis and by both inulin or oligofructose in low fat milk.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Lactobacillus; Bifidobacterium; Fructooligosaccharide; Fermented milk.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200014
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Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice BJM
Vasconcelos,Ana Luiza Sampaio; Nicoli,Jacques Robert; Nardi,Regina Maria Drummond.
In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Salmonella; Lactobacillus; Gnotobiotic mice.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
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Produções de ácido acético, etanol e dos isômeros óticos do ácido lático por linhagens de Lactobacillus isoladas de fermentações alcoólicas industriais Ciência e Agrotecnologia
Costa,Vanessa Moreira; Basso,Thiago Olitta; Angeloni,Luis Henrique Poleto; Oetterer,Marilia; Basso,Luiz Carlos.
Avaliaram-se no presente trabalho, as produções de etanol e dos ácidos acético e lático, bem como das proporções dos isômeros óticos D(-) e L(+) desse último, por 17 linhagens de Lactobacillus isoladas de fermentações industriais de produção de etanol. As linhagens foram crescidas a 32ºC por 24 horas, em meio contendo 1% de glucose, 1% de frutose, 1% de extrato de levedura, sais nutrientes (K, Mg e Mn) e tampão fosfato. Foram estimados os teores de ácido lático, ácido acético e etanol mediante cromatografia líquida de alta eficiência, assim como dos isômeros óticos D(-) e L(+) do ácido lático mediante espectrofotometria ao ultra-violeta, empregando desidrogenases láticas estereoespecíficas. O crescimento bacteriano foi inferido pela absorvância a 600 nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Fermentações Homo- e Heterolática; Ácido Acético; Ácidos D(-) e L(+) Lático; Etanol.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000200025
Registros recuperados: 52
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