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| Registros recuperados: 59 | |
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| Motta,R.G.; Ribeiro,M.G.; Perrotti,I.B.M.; Motta,D.G.; Domingues,P.F.; Lucas,T.M.; Zamprogna,T.O.; Listoni,F.J.P.. |
| An uncommon outbreak of mastitis caused by Arcanobacterium pyogenes in 26 cows is reported. The epidemiological findings, clinical signs, microbiological exams, somatic cell count, in vitro susceptibility profile of strains, efficacy of intramammary treatment and control measures were discussed. Florfenicol (96.2%), cefoperazona (92.3%), cefaloxin (84.6%) and ceftiofur (84.6%) were the most effective antimicrobials, and neomicin (27.0%) and enrofloxacin (17.4%) the least effective antimicrobials. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cattle; Arcanobacterium pyogenes; Outbreak; Mastitis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300027 |
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| Santos,V.J.C.; Simplício,K.M.M.G.; Sanchez,D.C.C.; Almeida,V.T.; Teixeira,P.P.M.; Coutinho,L.N.; Rodrigues,L.F.S.; Oliveira,M.E.F.; Feliciano,M.A.R.; Vicente,W.R.R.. |
| Objetivou-se descrever os achados clínicos e ultrassonográficos de uma cabra leiteira apresentando mastite gangrenosa. Com a ultrassonografia convencional, observaram-se alterações consistentes com o avanço do quadro clínico, como alterações na ecogenicidade do leite e parênquima mamário. A ultrassonografia Doppler revelou aumento da velocidade de pico sistólico nos três momentos estudados, passando de 15 para 17 e 29cm/s. Houve redução, também, dos valores do índice de resistência, de 0,71 para 0,61 e 0,43. O índice de pulsatividade sofreu redução entre o segundo e o terceiro dia de observação, passando de 0,98 para 0,71. Foi possível estabelecer relação direta entre os achados ultrassonográficos e a gravidade da doença. A ultrassonografia convencional e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goats; Mastitis; Ultrasonography; B mode; Doppler. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601931 |
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| Belli,C.B.; Fonte,J.S.; Torres,L.N.; Gondin,M.R.; Maiorka,P.C.; Fernandes,C.B.. |
| ABSTRACT Mammary disorders in mares are rare and usually only one animal per paddock is affected. In this report, two mares with 7 and 9 years old, were concomitantly diagnosis of chronic pyogranulomatous mastitis, associated with the Splendore-Hoeppli reaction, indicative of botryomycosis a rare, chronic suppurative disease with microabscess formation, characterized by in vivo formation of eosinophilic materials around microorganisms or biologically inert material. Various bacteria can cause botryomycosis in horses, and the most frequently isolated one is Staphylococcus sp., particularly S. aureus. This report confirms the role of Staphylococcus sp.; however, specifically S. hyicus and S. cohnii that prior to the current report, had not been associated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine; Mastitis; Mammary gland; Botryomycosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200342 |
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| Blagitz,M.G.; Benites,N.R.; Melville,P.A.; Batista,C.F.; Betiol,P.S.; Azedo,M.R.; Gomes,V.; Souza,F.N.; Della Libera,A.M.M.P.. |
| Avaliou-se o risco de infecção em diferentes fases da lactação em 33 ovelhas da raça Santa Inês. Inicialmente a glândula foi submetida ao exame físico e à prova de fundo escuro. Posteriormente, amostras de leite foram coletadas assepticamente para a realização de exame bacteriológico - California Mastitis teste -, e contagens microscópica e automática de células somáticas. Nenhuma diferença foi observada entre as distintas fases de lactação. Observou-se alta persistência de infecções intramamárias, e tendência a maior contagem de células somáticas no último período de lactação, que pode ser oriunda da maior resistência a infecções neste período. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ewe; Somatic cell count; Lactation stage; Mastitis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200034 |
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| Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo. |
| A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009 |
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| Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap. |
| This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009 |
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| Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes. |
| The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018 |
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| Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da. |
| The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535 |
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| Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de. |
| Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118 |
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| Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto. |
| The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050 |
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| Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V.. |
| ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801 |
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| Brito,Marilene de Farias; Seppa,Gilberto Santos; Teixeira,Luisa Gouvêa; Rocha,Thais Gomes; França,Ticiana do Nascimento; Hess,Tanja Maria; Peixoto,Paulo Vargas. |
| A non-lactating 17-year-old grey barren Mangalarga Marchador mare was referred to the Large Animal Veterinary Hospital of the Federal Rural University of Rio de Janeiro (UFRRJ), presenting enlargement of the mammary gland. The owner reported that the animal had a lesion in the mammary gland for at least two years, diagnosed and treated as chronic mastitis. Initially only the right gland was involved, presenting ulceration and exudation on the cutaneous surface. After 20 months, the left gland also became affected. The diagnosis of tubulo-papillary adenocarcinoma was based on the typical microscopic lesions. This work shows the importance of the histopathological examination in the differential diagnosis between the neoplasms and the chronic inflammation in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine diseases; Mammary neoplasms; Mastitis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200045 |
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| Vaz,Adil Knackfuss; Jones,James Eirug Thomas. |
| SUMMARY Ovine mastitis causes varying economic fosses in all countries where sheep are kept. A survey of 3128 breeding ewes in 22 farms in two areas with different management practices in Southern Brazil showed that the average prevalence of subclinical and chronic mastitis was 14.1% of ewes with at least one affected gland. From those, 4.49% were also bacteriologically positive. The prevalence in individual farms varied from 0 to 37% of ewes with clinical abnormalities of the udder. The overall prevalence was greater in Area II, where meat breeds predominate and animals are housed at night. It is concluded that subclinical and chronic mastitis is uniikely to be an economic problem in Area I, where wool production is the objective of the sheep industry.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sheep; Ewes; Mastitis; Lambs; Brazil. |
| Ano: 1994 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000200022 |
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| Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia. |
| ABSTRACT: We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452 |
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| Registros recuperados: 59 | |
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