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Registros recuperados: 69 | |
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Jean, Claire; Bourjea, Jerome; Jouen, Emmanuel; Taquet, Marc. |
Stock structure of swordfish (Xiphias gladius Linnaeus, 1758) from four areas of the South West Indian Ocean was conducted using Restriction Fragment Length Polymorphism (RFLP) on mtDNA control region and six amplified microsatellite loci. No significant differences were found among the four locations using either molecular technique. Most variability was found within populations, with only a small proportion of variation among samples, suggesting a unique stock of swordfish in the South West Indian Ocean. The sample size of this study is too small to reveal significant structure among the sampling locations. These results are baseline for a future larger scale project in the whole Indian Ocean. |
Tipo: Text |
Palavras-chave: Indian Ocean; Microsatellite loci; MtDNA; Xiphias gladius; Swordfish. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2313.pdf |
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Muths, Delphine; Tessier, Emmanuel; Gouws, G.; Craig, M.; Mwale, M.; Mwaluma, J.; Mwandya, A.; Bourjea, Jerome. |
The reef fish Myripristis berndti (Jordan & Everman 1903) is a pantropical species. A genetic analysis was conducted on 353 individuals from 10 localities distributed across the SW Indian Ocean (SWIO) in order to determine patterns of connectivity in the SWIO. Both the mtDNA sequences (711-bp cytochrome b sequences) and the microsatellites (8 newly developed loci) reveal spatial patterns of differentiation within the SWIO. There is, however, a discrepancy between the structure observed with each kind of marker. MtDNA revealed that 3 peripheral populations (NW Kenya, SE Reunion, and SW Europa) were isolated from the 7 more central populations, which form a more densely connected population network, while microsatellite data indicated a more restricted... |
Tipo: Text |
Palavras-chave: MtDNA; Microsatellite; Marine fish; SW Indian Ocean; Connectivity. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00073/18444/15974.pdf |
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Klossa-Kilia, E.; Kilias, G.; Sfenthourakis, S.. |
We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the... |
Tipo: Article / Letter to the editor |
Palavras-chave: Oniscidea; Greece; MtDNA; RLFPs; Genetic isolation; Ligidium; Isopods; 16S rDNA; 12S rDNA; 42.74. |
Ano: 2005 |
URL: http://www.repository.naturalis.nl/record/209650 |
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Kundrata, R.; Bocakova, M.; Bocak, L.. |
The Artematopodidae is a species-poor beetle family with contentious relationships to byrrhoid and elateroid families. Recent molecular phylogenetic analyses brought ambiguous results based on a single sequenced species. We investigated the taxonomic placement of Artematopodidae within Elateriformia using ribosomal (18S, 28S) and mitochondrial (rrnL, cox1) molecular markers and three artematopodid species. Our analyses placed Artematopodidae close to Omethidae+Telegeusidae in a basal position of broadly defined Elateroidea. Additionally, we described the first artematopodid species from China – Eurypogon jaechi sp. nov. and E. heishuiensis sp. nov. These species are reported from mountains of Yunnan and can be easily distinguished from their Palaearctic... |
Tipo: Article / Letter to the editor |
Palavras-chave: MtDNA; RDNA; Diversity; Elateriformia; New species; Palaearctic Region; Phylogeny; Taxonomy; 42.75; 42.64. |
Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/462591 |
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Arntzen, J.W.; Jehle, R.; Bardakci, F.; Burke, T.; Wallis, G.P.. |
Hybridization between divergent lineages often results in reduced hybrid viability. Here we report findings from a series of independent molecular analyses over several seasons on four life stages of F1 hybrids between the newts Triturus cristatus and T. marmoratus. These two species form a bimodal hybrid zone of broad overlap in France, with F1 hybrids making up about 4% of the adult population. We demonstrate strong asymmetry in the direction of the cross, with one class (cristatus-mothered) making up about 90% of F1 hybrids. By analyzing embryos and hatchlings, we show that this asymmetry is not due to prezygotic effects, as both classes of hybrid embryos are present at similar frequencies, implicating differential selection on the two hybrid classes... |
Tipo: Article / Letter to the editor |
Palavras-chave: Allozyme; Bateson–Dobzhansky–Muller incompatibility; Cytonuclear incompatibility; Hybridization; Microsatellite; MtDNA; Postzygotic isolation asymmetry; Triturus; 42.82. |
Ano: 2008 |
URL: http://www.repository.naturalis.nl/record/289893 |
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Vargas-Ramírez, M.; Chiari, Y.; Castaño-Mora, O.V.; Menken, S.B.J.. |
The Magdalena River Turtle (Podocnemis lewyana) is a Colombian endemic species, endangered due to human exploitation and habitat destruction. To date, this species is poorly known ecologically and data on its genetic diversity are lacking. Here we report on the first genetic survey of the species across its distribution range. We obtained mitochondrial DNA sequences (488 bp) of the cytochrome b gene from 109 individuals. Samples belong to populations located at several different localities, grouped in five regions, along the four main river basins: Magdalena, Cauca, San Jorge, and Sinú drainages. We found two haplotypes, which differ in only one nucleotide substitution and which are represented with different frequencies in the five geographic regions.... |
Tipo: Article / Letter to the editor |
Palavras-chave: Reptilia; Genetic population structure; Cytochrome b; MtDNA; Podocnemis; 42.82. |
Ano: 2007 |
URL: http://www.repository.naturalis.nl/record/217421 |
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Arntzen, J.W.; Wallis, Graham P.. |
Within the newt genus Triturus, the large-bodied species in the T. cristatus (crested newt) superspecies show an unusual degree of variation in relative trunk length as a result of among-taxon variation in interlimb vertebral count. Here we examine the systematic value of this feature as assessed by both exterior measurement (Wolterstorff Index) and direct radiographic count of rib-bearing vertebrae, with particular reference to a number of confounding factors (sex differences, hybridisation, geographic variation, allometry, preservation effects). Using our mtDNA haplotype data, which are largely concordant with geographic distribution of species, we find that direct count of the rib-bearing vertebrae performs more reliably (14% misclassification) than... |
Tipo: Article / Letter to the editor |
Palavras-chave: Asymmetric hybridisation; Biogeography; Crested newt; Geographic variation; Morphology; MtDNA; Taxonomy; Triturus cristatus. |
Ano: 1999 |
URL: http://www.repository.naturalis.nl/record/534387 |
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Moretto,Geraldo; Arias,Maria C.. |
The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP).... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004 |
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García-Machado, E.; Chevalier, P.; Solignac, M.. |
The hamlets are a group of vividly colored fish species of the Serranidae family differentiated only by the color pattern of the body. Although there are divergent views about hamlet taxonomy, experimental and field observations have shown a strong assortative mating, justifying a species status for the different color morphs. Here we analyze the level of evolutionary divergence among six species in respect of mitochondrial DNA, with a view to contrasting the pattern observed with color partitions and previous results obtained with isozymes. The estimated molecular distance among species was low and of the same magnitude as nucleotide diversity within species. Consequently, the net distance and hence the time of divergence between taxa was virtually zero... |
Tipo: Journal Contribution |
Palavras-chave: MtDNA; Marine fish; Genetic diversity. |
Ano: 2003 |
URL: http://hdl.handle.net/1834/4569 |
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Hernández Martínez, D.; Álvarez-Alemán, A.; Bonde, R. K.; Powell, J.A.; García-Machado, E.. |
El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13 individuos, 12 de Cuba y uno procedente de la Florida, EU. Las secuencias de un fragmento de 410 pb de la región de control del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como endémico de Belice, se encontró en un individuo... |
Tipo: Journal Contribution |
Palavras-chave: Genetic diversity; MtDNA. |
Ano: 2013 |
URL: http://hdl.handle.net/1834/5252 |
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MÁRQUEZ-ACERO,ANGELA SABRINA; VIDAL-GARCIA,JUAN MANUEL; GALINDO,LUIS FRANCISCO BECERRA; GARCÍA,ALEXANDER GARCÍA. |
ABSTRACT Taxonomic identification of the species involved in the processes of cadaveric decomposition is a fundamental procedure in forensic entomological analysis. Among the species involved in the processes of decay, those of the Calliphoridae family are particularly important because they come to the body in the early stages of decomposition. The aim of this research is to identify six species of Calliphoridae (Calliphora nigribasis, Calliphora vicina, Compsomyopsis verena, Sarconesiopsis magellanica, Chrysomia albiceps and Roraimomusca roraima) with forensic interest found in Bogotá. For that, sequences of 599 bp from mitochondrial gene COII wereas obtained. The identification was made by analysis of genetic distances under Jukes-Cantor model. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Forensic entomology; Barcode; MtDNA; COII. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492017001200141 |
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Specht,Alexandre; Sosa-Gómez,Daniel Ricardo; Paula-Moraes,Silvana Vieira de; Yano,Silvia Akimi Cavaguchi. |
O objetivo deste trabalho foi descrever métodos para a caracterização morfológica e molecular de Helicoverpa armigera e ampliar o registro de ocorrência da praga no Brasil. As mariposas foram obtidas de lagartas coletadas nas culturas de algodão, milho e soja, com uso de armadilhas luminosas. As coletas foram realizadas na Bahia, no Distrito Federal, no Mato Grosso e no Paraná. A identificação foi baseada na genitália masculina e nas análises das sequências dos genes mitocondriais do citocromo B e da região cox1-tRNALeu-cox2. A genitália masculina foi comparada com as descrições morfológicas na literatura, e as sequências de genes, com as depositadas no GenBank. Ambas as análises confirmaram a presença de H. armigera nos locais de coleta. Ampliou-se o... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Código de barras de DNA; Lagarta polífaga; MtDNA; Praga quarentenária. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000600015 |
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Zhang,T; Du,W; Lu,H; Wang,L. |
ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chinese Black-bone Chicken; MtDNA; D-loop region; Haplotype; Maternal origin. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300565 |
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VÉLIZ,DAVID; CATALÁN,LAURA; PARDO,RODRIGO; ACUÑA,PATRICIO; DÍAZ,ANGIE; POULIN,ELIE; VILA,IRMA. |
There is still doubt as to the number of species of the freshwater Chilean ichthyofauna, 64 % of which have conservation problems. One of the groups is that of the silversides of the genus Basilichthys. Three morphological species of this genus have been described in Chile with disjoint distributions: Basilichthys semotilus, B. microlepidotus and B. australis; the latter two overlap in distribution only in the Aconcagua River and are not easily distinguishable by morphological and meristic characters. In order to evaluate the efficacy of identification of these species by molecular techniques, we analyzed the sequence of 9 % of the mitochondrial DNA (Control Region and COI) of individuals from the Loa River (21°41' S) to the Valdivia River (39°50' S),... |
Tipo: Journal article |
Palavras-chave: COI; Control Region; Drainages; MtDNA; Silverside fish. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100004 |
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Registros recuperados: 69 | |
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