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	Provedor de dados BJM (15) BABT (5) PAB (4) Infoteca-e (3) Anais da ABC (AABC) (3) Genet. Mol. Biol. (3) R. Bras. Zootec. (3) Trop. Plant Pathol. (2) Arq. Bras. Med. Vet. Zootec. (2) ArchiMer (2) Electron. J. Biotechnol. (2) Agronomy (1) Rev. Protección Veg. (1) Acta Amazonica (1) BJID (1) BJMBR (1) Ciência Rural (1) J. Venom. Anim. Toxins incl. Trop. Dis. (1) Rev. Bras. Bot. (1) Rev. Bras. Ciênc. Avic. (1) Mais... Autor Martins,Meire Lelis Leal (3) Barbosa,Nei André Arruda (2) Brandelli,Adriano (2) Branquinha,Marta H. (2) Cabral,Hamilton (2) D'Avila-Levy,Claudia M. (2) Nascimento,Wellingta Cristina Almeida do (2) Porto,Ana Lúcia Figueiredo (2) Sakomura,Nilva Kazue (2) Santos,André L.S. (2) ANDRIOLI, A. (1) AVILA, V. S. de (1) Adnan,Ahmad (1) Ahmad,Waqar (1) Ahmed,M.Fazil (1) Alberto,Raiani Nascimento (1) Albino,Luiz Fernando Teixeira (1) Almeida,Tiago Tognolli de (1) Alves,Luana de Fátima (1) Alves,Maria Helena (1) Mais... Palavra-chave Protease (53) Lipase (7) Amilase (6) Amylase (3) Celulase (3) Fitase (3) Thermophilic bacterium (3) Bacillus sp. (2) Characterization (2) Chitinase (2) Enzyme (2) Frango de corte (2) Inhibitor (2) Purification (2) Rhizoctonia solani (2) Solid-state fermentation (2) Xilanase (2) 5.01.02.04-4 microbial enzyme (1) Actinobacillus actinomycetemcomitans (1) Activity and Stability (1) Mais... Tipo do documento Info:eu-repo/semantics/article (43) Journal article (3) Text (2) Artigo de divulgação na mídia (INFOTECA-E) (1) Comunicado Técnico (INFOTECA-E) (1) Documentos (INFOTECA-E) (1) Info:eu-repo/semantics/report (1) Pesquisa de campo; Laboratório (1) Mais... Ano 2020 (1) 2019 (2) 2017 (3) 2016 (2) 2015 (7) 2014 (1) 2013 (1) 2012 (2) 2011 (6) 2010 (2) 2009 (4) 2008 (2) 2007 (2) 2006 (5) 2005 (5) 2004 (3) 2003 (1) 2002 (2) 2001 (1) 1999 (1) Mais... País Brazil (48) Chile (2) France (2) Cuba (1) Idioma Inglês (36) Português (16) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 53 Primeira ... 1 2 3 ... Última Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization BJM Sangeetha,R.; Geetha,A.; Arulpandi,I.. This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus licheniformis; Protease; Lipase; Concomitant production; Purification. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026 Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease BJM Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo. Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen. have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase, polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%). Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009 Effect of the culture conditions on the production of an extracellular protease by thermophilic Bacillus sp and some properties of the enzymatic activity BJM Silva,Camila Rocha da; Delatorre,Andréia Boechat; Martins,Meire Lelis Leal. Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing 1% maltose as a carbon source and supplemented with whey protein (0.1%) and corn steep liquor (0.3%) reached a maximum at 14 h, with levels of 42 U/mg protein. The microorganism was capable of utilizing a wide range of carbon sources, but protease activity varied according the carbon source. Starch and maltose were the best carbon sources in the present study for protease secretion, while lactose and sucrose were less effective. Increasing maltose concentration in the medium until 1%, improved the growth of the organism and the enzyme activity. Regarding the amounts of corn steep liquor and whey protein in the medium, the concentrations of 0.2% and 0.1%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200012 Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN BJM Dhawan,Manish; Joshi,Neelam. Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Entomopathogenic fungi; Lipase; Mortality; Protease. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300522 Studies on the stability of protease from Bacillus sp. and its compatibility with commercial detergent BJM Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal. Enzymes, and particularly proteases, have become an important and indispensable part of industrial processes such as laundry detergents, pharmaceuticals and food products. Detergents such as Tide®, Ariel® and Biz® contain proteolytic enzymes, most of them produced by members of the genus Bacillus. This paper describes the compatibility of protease produced by the thermophilic Bacillus sp, with commercial laundry detergent. Stability studies indicated that this enzyme retained about 95% and 74% of its maximum activity after 1h at 60ºC in the presence of glycine in combination with MnSO4 and CaCl2, respectively. No inhibitory effect was observed at 1.0-5.0 mM of EDTA. Triton X-100 inhibited the enzyme in all the concentrations tested. The enzyme was unstable... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.; Detergents. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300020 Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts BJM Oliveira,Lilian C.G.; Ramos,Patricia Locosque; Marem,Alyne; Kondo,Marcia Y.; Rocha,Rafael C.S.; Bertolini,Thiago; Silveira,Marghuel A.V.; Cruz,João Batista da; Vasconcellos,Suzan Pantaroto de; Juliano,Luiz; Okamoto,Debora N.. Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Halophilic; Protease; Lipase; Amylase; Cellulase. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200347 Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk BJM Corrêa,Ana Paula F.; Daroit,Daniel J.; Velho,Renata V.; Brandelli,Adriano. The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High levels of α-glucosidase were produced in skim milk broth. Hydrolytic enzymes detected in skim milk broth are of particular concern, indicating that these enzymes could be produced by Pseudomonas sp. during the cold storage of raw milk, contributing to the spoilage problem in milk and dairy products. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas; Hydrolases; Protease; Lipase; Glycosidase. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400034 Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach BJM Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M.. Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. Tipo: Info:eu-repo/semantics/article Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design (CCD). Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures BJM Moreira,Fabiana Guillen; Reis,Simone dos; Costa,Maria Aparecida Ferreira; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina. The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC. Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrases; Hydrolytic enzymes; Myrothecium verrucaria; Protease. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100002 Production and properties of an extracellular protease from thermophilic Bacillus sp BJM Nascimento,Wellingta Cristina Almeida do; Martins,Meire Lelis Leal. Protease production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing trisodium citrate reached a maximum in 9h, with levels of 1.93U/mg protein. The microorganism utilized several carbon sources for the production of protease. Starch was the best substrate, followed by trisodium citrate, citric acid and sucrose. Among the various organic and inorganic nitrogen sources, ammonium nitrate was found to be the best. Studies on the protease characterization revealed that the optimum temperature of this enzyme was 60ºC. The enzyme was stable for 2h at 30ºC, while at 40ºC and 80ºC, 14% and 84% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be 8.0. After incubation of crude enzyme... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Thermophilic bacterium; Bacillus sp.. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100015 Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes BJM Gomaa,Eman Zakaria. The potentiality of 23 bacterial isolates to produce alkaline protease and carboxymethyl-cellulase (CMCase) on Ficus nitida wastes was investigated. Bacillus pumillus ATCC7061 was selected as the most potent bacterial strain for the production of both enzymes. It was found that the optimum production of protease and CMCase were recorded at 30 °C, 5% Ficus nitida leaves and incubation period of 72 h. The best nitrogen sources for protease and CMCase production were yeast extract and casein, respectively. Also maximum protease and CMCase production were reported at pH 9 and pH 10, respectively. The enzymes possessed a good stability over a pH range of 8-10, expressed their maximum activities at pH10 and temperature range of 30-50 °C, expressed their maximum... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaliphillic; Protease; Carboxymethylcellulase; Production; Characterization. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200033 Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans BJM Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa. Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008 Enzimas exógenas em dietas de frangos de corte: desempenho Ciência Rural Barbosa,Nei André Arruda; Sakomura,Nilva Kazue; Bonato,Melina Aparecida; Hauschild,Luciano; Oviedo-Rondon,Edgar. O experimento foi conduzido para avaliar a eficiência de enzimas exógenas em dietas sobre o desempenho de frangos de corte. Um total de 1440 pintos de corte machos (Cobb 500®) foram distribuídos em um delineamento inteiramente casualizado em esquema fatorial 2x2 (duas dietas com diferentes densidades nutricionais; com e sem a adição enzimática) com 8 repetições de 45 aves em cada unidade experimental. As dietas com diferentes densidades nutricionais foram: controle positivo (níveis normais) e um controle negativo (com redução de 4,3 e 4,5% de energia metabolizável; 16,7 e 17,7% de cálcio e 35 e 42,7% de fósforo, nas fases inicial e de crescimento, respectivamente). A suplementação enzimática consistiu da combinação das enzimas fitase (100g t-1) e amilase,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aves; Fitase; Amilase; Protease; Xilanase. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800027 Registros recuperados: 53 Primeira ... 1 2 3 ... Última

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