|
|
|
| Registros recuperados: 66 | |
|
| |
|
| |
|
| |
|
| |
|
|
| Manfredini,V.; Roehrs,R.; Peralba,M.C.R.; Henriques,J.A.P.; Saffi,J.; Ramos,A.L.L.P.; Benfato,M.S.. |
| Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2delta mutant, while the sod1deltasod2delta double mutant was not sensitive. Sod mutants had lower catalase activity (44%) than wild-type cells, independent of H2O2 stress. Untreated cells of sod1deltasod2delta double mutants showed increased glutathione peroxidase activity (126%), while sod1delta had lower activity (77%) than the wild type. Glutathione levels in sod1delta were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Hydrogen peroxide; Saccharomyces cerevisiae; Reactive oxygen species. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200001 |
| |
|
|
| Sai,Ma; Le-qun,Shan; Yu-hong,Xiao; Fang,Li; Li,Huang; Shen,Lijuan; Ji-hua,Chen. |
| Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quaternary ammonium compounds; Dental monomer; Cytotoxicity; Apoptosis; Reactive oxygen species; Methacryloxylethyl cetyl ammonium chloride. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100008 |
| |
|
|
| Leonelli,M.; Graciano,M.F.R.; Britto,L.R.G.. |
| The transient receptor potential channels family (TRP channels) is a relatively new group of cation channels that modulate a large range of physiological mechanisms. In the nervous system, the functions of TRP channels have been associated with thermosensation, pain transduction, neurotransmitter release, and redox signaling, among others. However, they have also been extensively correlated with the pathogenesis of several innate and acquired diseases. On the other hand, the omega-3 polyunsaturated fatty acids (n-3 fatty acids) have also been associated with several processes that seem to counterbalance or to contribute to the function of several TRPs. In this short review, we discuss some of the remarkable new findings in this field. We also review the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TRP channels; Calcium; Omega-3 fatty acids; Reactive oxygen species; G-protein coupled receptors; Neurodegeneration. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100003 |
| |
|
| |
|
| |
|
| |
|
|
| Paino,I.M.M.; Ximenes,V.F.; Fonseca,L.M. da; Kanegae,M.P.P.; Khalil,N.M.; Brunetti,I.L.. |
| The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-steroidal anti-inflammatory drugs; Neutrophils; Myeloperoxidase; Hypochlorous acid; Reactive oxygen species; Chemiluminescence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400007 |
| |
|
|
| Zhang,You En; Huang,Guang Qing; Wu,Bing; Lin,Xin Duo; Yang,Wen Zi; Ke,Zun Yu; Liu,Jie. |
| Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; ROS; Hydrogen sulfide; H2O2-induced apoptosis; Cardiomyocytes. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400608 |
| |
|
|
| Sunlight is part of our everyday life and most people accept it as beneficial to our health. With the advance of our knowledge in cutaneous photochemistry, photobiology and photomedicine over the past four decades, the terrestrial solar radiation has become a concern of dermatologists and is considered to be a major damaging environmental factor for our skin. Most photobiological effects (e.g., sunburn, suntanning, local and systemic immunosuppression, photoaging or dermatoheliosis, skin cancer and precancer, etc.) are attributed to ultraviolet radiation (UVR) and more particularly to UVB radiation (290-320 nm). UVA radiation (320-400 nm) also plays an important role in the induction of erythema by the photosensitized generation of reactive oxygen species... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sunburn; Antioxidant; DNA photodamage; Reactive oxygen species; UV radiation. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500002 |
| |
|
|
| Belizário,J.E.; Alves,J.; Occhiucci,J.M.; Garay-Malpartida,M.; Sesso,A.. |
| Mitochondria increase their outer and inner membrane permeability to solutes, protons and metabolites in response to a variety of extrinsic and intrinsic signaling events. The maintenance of cellular and intraorganelle ionic homeostasis, particularly for Ca2+, can determine cell survival or death. Mitochondrial death decision is centered on two processes: inner membrane permeabilization, such as that promoted by the mitochondrial permeability transition pore, formed across inner membranes when Ca2+ reaches a critical threshold, and mitochondrial outer membrane permeabilization, in which the pro-apoptotic proteins BID, BAX, and BAK play active roles. Membrane permeabilization leads to the release of apoptogenic proteins: cytochrome c, apoptosis-inducing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial outer membrane permeabilization; Permeability transition pore; Cytochrome c; Reactive oxygen species; Caspases; BCL-2. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800001 |
| |
|
|
| Touyz,R.M.; Berry,C.. |
| Angiotensin II (Ang II)* is a multifunctional hormone that influences the function of cardiovascular cells through a complex series of intracellular signaling events initiated by the interaction of Ang II with AT1 and AT2 receptors. AT1 receptor activation leads to cell growth, vascular contraction, inflammatory responses and salt and water retention, whereas AT2 receptors induce apoptosis, vasodilation and natriuresis. These effects are mediated via complex, interacting signaling pathways involving stimulation of PLC and Ca2+ mobilization; activation of PLD, PLA2, PKC, MAP kinases and NAD(P)H oxidase, and stimulation of gene transcription. In addition, Ang II activates many intracellular tyrosine kinases that play a role in growth signaling and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Angiotensin receptors; Second messengers; Phospholipase; Protein kinase; Reactive oxygen species. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900001 |
| |
|
|
| Grando,F.C.C.; Felício,C.A.; Twardowschy,A.; Paula,F.M.; Batista,V.G.; Fernandes,L.C.; Curi,R.; Nishiyama,A.. |
| To determine the effects of saturated and unsaturated fatty acids in phosphatidylcholine (PC) on macrophage activity, peritoneal lavage cells were cultured in the presence of phosphatidylcholine rich in saturated or unsaturated fatty acids (sat PC and unsat PC, respectively), both used at concentrations of 32 and 64 µM. The treatment of peritoneal macrophages with 64 µM unsat PC increased the production of hydrogen peroxide by 48.3% compared to control (148.3 ± 16.3 vs 100.0 ± 1.8%, N = 15), and both doses of unsat PC increased adhesion capacity by nearly 50%. Moreover, 64 µM unsat PC decreased neutral red uptake by lysosomes by 32.5% compared to the untreated group (67.5 ± 6.8 vs 100.0 ± 5.5%, N = 15), while both 32 and 64 µM unsat PC decreased the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phosphatidylcholine; Lecithin; Macrophages; Fatty acids; Reactive oxygen species; Phagocytosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700003 |
| |
|
|
| Touyz,R.M.. |
| Diseases such as hypertension, atherosclerosis, hyperlipidemia, and diabetes are associated with vascular functional and structural changes including endothelial dysfunction, altered contractility and vascular remodeling. Cellular events underlying these processes involve changes in vascular smooth muscle cell (VSMC) growth, apoptosis/anoikis, cell migration, inflammation, and fibrosis. Many factors influence cellular changes, of which angiotensin II (Ang II) appears to be amongst the most important. The physiological and pathophysiological actions of Ang II are mediated primarily via the Ang II type 1 receptor. Growing evidence indicates that Ang II induces its pleiotropic vascular effects through NADPH-driven generation of reactive oxygen species (ROS).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vascular smooth muscle cells; Remodeling; Inflammation; Signal transduction; Reactive oxygen species. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800018 |
| |
|
|
| Santos,Alexandra de Andrade; Silveira,Joaquim Albenísio Gomes da; Bonifacio,Aurenivia; Rodrigues,Artenisa Cerqueira; Figueiredo,Márcia do Vale Barreto. |
| Abstract Soil salinity is an important abiotic stress worldwide, and salt-induced oxidative stress can have detrimental effects on the biological nitrogen fixation. We hypothesized that co-inoculation of cowpea plants with Bradyrhizobium and plant growth-promoting bacteria would minimize the deleterious effects of salt stress via the induction of enzymatic and non-enzymatic antioxidative protection. To test our hypothesis, cowpea seeds were inoculated with Bradyrhizobium or co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and then submitted to salt stress. Afterward, the cowpea nodules were collected, and the levels of hydrogen peroxide; lipid peroxidation; total, reduced and oxidized forms of ascorbate and glutathione; and superoxide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant-bacteria interactions; Reactive oxygen species; Salinity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300513 |
| |
|
|
| Vellosa,José Carlos Rebuglio; Khalil,Najeh Maissar; Gutierres,Vânia Ortega; Santos,Vânia Aparecida de Freitas Formenton Macedo dos; Furlan,Maysa; Brunetti,Iguatemy Lourenço; Oliveira,Olga Maria Mascarenhas de Faria. |
| Reactive oxygen species (ROS) and free radical species have been implicated in initiating or accompanying many diseases in living organisms; there is thus, a continual need for antioxidants molecules to inactivate ROS/free radicals. Many studies of plants crude extracts have demonstrated free-radical scavenging and antioxidant action. Salacia species have long been used, in several countries, as traditional medicines against certain diseases and for their anti-inflammatory properties. In this study, Salacia campestris Walp (Hippocrateaceae) root bark ethanol extract (ScEtOH) was assessed for its ability to scavenge free radicals and reactive oxygen species; the results were expressed as percentage inhibition of the active species. ScEtOH was efficient... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salacia campestris Walp/antioxidant properties; Hippocrateaceae; Myeloperoxidase; Reactive oxygen species; Antioxidants; Free radicals. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100012 |
| |
|
| |
| Registros recuperados: 66 | |
|
|
|
