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Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea ArchiMer
Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf
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Genetical improvement of marine fish and shellfish : a french perspective ArchiMer
Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre.
In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed: •The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the...
Tipo: Text Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf
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Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas? ArchiMer
Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C..
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf
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Early life behavioural differences in wild caught and domesticated sea bass (Dicentrarchus labrax) ArchiMer
Benhaim, David; Pean, Samuel; Lucas, Gael; Blanc, Nancy; Chatain, Beatrice; Begout, Marie-laure.
Behavioural studies comparing hatchery and wild-caught fish are useful to improve selection for aquaculture and restocking programmes. We examined swimming behaviour characteristics in wild captured and domesticated sea bass juveniles before and after eliciting a startle response at 8 different ages and always on naive individuals. We specifically investigated whether domestication impacts juvenile sea bass behaviour and whether the first months of captivity induce behavioural modifications in wild juveniles. An apparatus was designed to mimic a predator attack by presenting a sudden visual and mechanical stimuli simultaneously in 8 arenas where single individuals were placed and video recorded. The reactivity response was evaluated and different swimming...
Tipo: Text Palavras-chave: Domestication; Swimming activity; Restocking; Selection; Coping styles.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00102/21363/19403.pdf
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Is avoiding full sib matings really worthwhile in a breeding program? ArchiMer
Dupont-nivet, Mathilde; Vandeputte, Marc.
Stochastic simulations were used to compare genetic progress and inbreeding depending on whether full sib matings were allowed or not when applying individual selection. Fifteen selection cycles were considered, using a single pair mating design. Two levels of heritability (h(2)) were studied (0.1; 0.5) as well as four different proportions of selected animals (2, 5, 10 and 20%), four population sizes (1000, 2,000, 4,000 and 10,000 candidates) and two levels of inbreeding depression (0 and 5% decrease of phenotype for 10% increase in inbreeding). Fifty replicates were generated for each set of parameters. The simulations revealed that avoiding full sib matings became less and less relevant as population size and proportion of selected animals increased and...
Tipo: Text Palavras-chave: Selection; Sib mating; Inbreeding; Genetic variability; Stochastic simulations; Fish.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00035/14587/11952.pdf
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Summer mortality in juvenile cupped oysters : response to selection in the field and in laboratory ArchiMer
Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Samain, Jean-francois; Boudry, Pierre.
The French project "Morest", coordinated by IFREMER, aims to a better understanding of the causes of summer mortalities in juvenile oysters by a multidisciplinary approach. Within this program, a first generation (G1) was bred in 2001 to assess to what extent genetic variability exists for this trait in French populations. 17 half-sib families (HSF) including 44 full-sib families (FSF) were obtained in the G1, and each FSF was reared in three sites along the French coast during the summer period. Significant differences in survival were observed among HSF, clearly indicating a genetic basis for survival. Estimation of heritability for survival was very high: 0.81  0.29. In 2002, 3 "good" HSF and 3 "bad" HSF were selected on their survival...
Tipo: Text Palavras-chave: Selection; Genetic; Mortalities; Crassostrea gigas; Oyster.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3439.pdf
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How to genetically increase fillet yield in fish: New insights from simulations based on field data ArchiMer
Fraslin, Clemence; Dupont-nivet, Mathilde; Haffray, Pierrick; Bestin, Anastasia; Vandeputte, Marc.
Fillet yield (i.e. the proportion of edible flesh from a given amount of fish) is a trait of high economic interest for species sold as fillets. Improving it by selective breeding is not an easy task, as it is a ratio trait, which causes mathematical difficulties. It is moreover a specific ratio where numerator (fillet weight) and denominator (body weight) are very strongly correlated (genetic and phenotypic correlations in the range 0.89–0.99). This led some authors to conclude that they have the same genetic and phenotypic basis, precluding selection for improved fillet yield. In this study, we propose to study fillet yield as a component of two traits, fillet weight and waste weight (waste weight = body weight-fillet weight, so the sum of head, bones,...
Tipo: Text Palavras-chave: Aquaculture; Genetics; Heritability; Selection; Processing yields.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00415/52599/53430.pdf
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Genetic variability in residual feed intake in rainbow trout clones and testing of indirect selection criteria ArchiMer
Grima, Laure; Quillet, E; Boujard, Thierry; Robert Granie, C; Chatain, Beatrice; Mambrini, M.
Little is known about the genetic basis of residual feed intake (RFI) variation in fish, since this trait is highly sensitive to environmental influences, and feed intake of individuals is difficult to measure accurately. The purpose of this work was (i) to assess the genetic variability of RFI estimated by an X-ray technique and (ii) to develop predictive criteria for RFI. Two predictive criteria were tested: loss of body weight during feed deprivation and compensatory growth during re-feeding. Ten heterozygous rainbow trout clones were used. Individual intake and body weight were measured three times at three-week intervals. Then, individual body weight was recorded after two cycles of a three-week feed deprivation followed by a three-week re-feeding....
Tipo: Text Palavras-chave: Selection; Indirect criteria; Residual feed intake; Clone; Rainbow trout.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4777.pdf
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Déclin de la taille-aux-âges des sardines (Sardina pilchardus) : diminution de la croissance et/ou disparition sélective des individus à croissance rapide ArchiMer
Gentil, Claire.
In many exploited species, we see a decrease of size-at-ages. Two main hypotheses have been proposed to explain this decline: a decline in growth and the selective mortality of the individuals with fast growth both being due to environmental changes or/and to fishing. The sardine Sardina pilchardus of the Bay of Biscay is a good model for the study of this phenomenon because of its high sensitivity to the environmental variations and it sustains two large French and Spanish fisheries. The aim of this study is to measure the changes in the growth of sardines, by the reading of otoliths, and to understand mechanisms involved in this process. The main stages of this study are described in this report: extraction of otoliths during PELGAS, otoliths...
Tipo: Text Palavras-chave: Sardina pilchardus; Golfe de Gascogne; Otolithes; Croissance; Mortalité; Sélection; Sardina pilchardus; Bay of Biscay; Otoliths; Growth; Mortality; Selection.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00459/57038/58942.pdf
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Temperature effect on survival, growth, and triacylglycerol content during the early ontogeny of Mytilus edulis and M. trossulus ArchiMer
Rayssac, Nathalie; Pernet, Fabrice; Lacasse, Olivia; Tremblay, Rejean.
Temperature is a major factor contributing to the biogeography of intertidal poikilotherms. The population dynamics of intertidal invertebrates like mussels are strongly dependent on larval supply and larval mortality. Unlike adults, which are resistant to fluctuating environmental conditions, larvae are highly sensitive to the physicochemical conditions of the pelagic zone. The effects of rearing temperature on larval and post-larval performance of 2 coexisting mussels species, Mytilus edulis and M. trossulus, were examined in this study. In the first experiment, larvae of both species were reared separately at constant temperatures of 10, 17, and 24 degrees C from D-larvae to the dissoconch stage. In the second experiment, pediveligers were reared under...
Tipo: Text Palavras-chave: Thermal adaptation; Bivalve mollusk; Selection; Biogeography; Lipid; Larval ecology.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13028/10159.pdf
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Anti-predator response of Haliotis tuberculata is modified after only one generation of domestication ArchiMer
Roussel, Sabine; Bisch, T; Lachambre, S; Boudry, Pierre; Gervois, Jl; Lambert, Christophe; Huchette, S; Day, R.
Domestication of Haliotis tuberculata has only recently begun. During the process, we expect that behavioural and physiological traits may evolve to become more adapted to their captive environment. These modifications may result from intentional selection of production traits or unconscious and unintentional selection due to conditions experienced in the farm environment. To study this process at the earliest stage, the progeny of 3 different broodstocks obtained from wild parents, selected farmed abalone and randomly sampled farmed abalone, were studied. After rearing for 16 mo in separate tanks, offspring from the 3 progenies were placed together in sea cages at the same density. After 3 yr, behavioural traits were studied, and the immune status after a...
Tipo: Text Palavras-chave: Predation; Hiding behaviour; Haliotis tuberculata; Domestication; Selection; Captive; Abalone.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59841/62989.pdf
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Evidence of response to unintentional selection for faster development and inbreeding depression in Crassostrea gigas larvae ArchiMer
Taris, Nicolas; Batista, F; Boudry, Pierre.
Underlying consequences of domestication and artificial selection still remain largely unexplored in most aquacultured species. For species with a two phase life cycle, including the Pacific oyster Crassostrea gigas, most genetic studies have focused on the post-metamorphosis juvenile and adult stages, but relatively few considered the larval stage. To assess the consequence of hatchery practices on larval characters, especially growth, we performed a phenotypic study on larval progenies derived from crosses between Pacific oysters from natural beds and farmed Pacific oysters selected for desirable production traits such as rapid growth, for over seven generations. A set of three microsatellite loci was used to compare the genetic variability between the...
Tipo: Text Palavras-chave: Crassostrea gigas; Inbreeding depression; Selection; Domestication; Larval stage.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3848.pdf
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Validation in commercial conditions of the response to selection of the European flat oyster Ostrea edulis for resistance to Bonamia ostreae ArchiMer
Bedier, Edouard; Langlade, Aime; Angeri, Stéphane; Brizard, Raphael; Nerlovic, V.; Glize, Philippe; Haffray, Philippe.
Since the outbreaks of the parasitosis with Marteilia refringens and Bonamia ostreae in the last 60's, the French production of the European flat oyster dropped from 20000 mT to less than 1500 mT currently, leaving the French shellfish farming in situation of virtually monoculture of the Pacific cupped oyster Crassostrea gigas. To sustain this traditional culture, Ifremer has developed, at the experimental level, a genetic selection for a better tolerance to Bonamia ostreae which led to an improvement of survivals. The transfer of these results to the business scale requires the control of the reproduction in hatchery and the adaptation of the cycle of production to the constraints of seedlings in deep water as practised in Brittany (France). That's why a...
Tipo: Text Palavras-chave: Selection; Genetic; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3313.pdf
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Response to selection for increasing resistance to the spring mortality outbreaks in Mytilus edulis occurring in France since 2014 ArchiMer
Degremont, Lionel; Maurouard, Elise; Rabiller, Manuella; Glize, Philippe.
Massive spring mortality outbreaks have been reported in Mytilus spp. in France since 2014. The main areas affected are the Pertuis Charentais Sounds and the coast of the Pays de la Loire, which cultivate mainly M. edulis, and the putative causal agents remain unknown. We report the results of the first generation of mass selection focused on survival and resistance to the spring mortality in M. edulis. Two stocks of mussels were sampled in the Pertuis Charentais Sounds in 2014; one of these went through a spring mortality outbreak and the survivors were used to produce the selected stock, while the second stock was M. edulis considered to be naïve against the putative causal agents of the mortality outbreak and was used to produce the control stock. A...
Tipo: Text Palavras-chave: Spring mortality; Survival; Mytilus edulis; Heritability; Selection.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61619/65530.pdf
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Exploration behaviour and flight response toward a stimulus in three sea bass strains (Dicentrarchus labrax L.) ArchiMer
Millot, Sandie; Begout, Marie-laure; Chatain, Beatrice.
Domestication and selection may affect fish behaviour, sometime as soon as at the first generation of domestication. However, knowledge about how both processes impact on fish spatial exploration and swimming activity still is to be improved. The objective of this experiment was (i) to evaluate spatial exploration behaviour and swimming activities of three sea bass strains having different domestication and selection levels and (ii) to analyse their responses to an acute stress. Sea bass exploration and swimming activities were studied before, during and 40 min after a stimulation (standardized fall of an object). The experimental tank was divided in to four zones, and the time spent, the distance travelled in each zone and the swimming complexity were...
Tipo: Text Palavras-chave: Thigmotaxis; Risk assessment; Danger avoidance; Swimming activity; Personality; Selection; Domestication.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6458.pdf
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Improving feed efficiency in fish using selective breeding: a review ArchiMer
De Verdal, Hugues; Komen, Hans; Quillet, Edwige; Chatain, Beatrice; Allal, Francois; Benzie, John A. H.; Vandeputte, Marc.
Improving feed efficiency (FE) is key to reducing production costs in aquaculture and to achieving sustainability for the aquaculture industry. Feed costs account for 30–70% of total production costs in aquaculture; much work has been done on nutritional and husbandry approaches to improve FE but only a limited amount of research has been devoted to using genetics, despite its potential. This paper reviews past work to improve FE in fish using selective breeding and assess future directions. Direct selection on FE traits requires methods to measure individual feed consumption and estimate FE efficiently and accurately. This is particularly difficult to do in fish because of the environment in which they live. Many of the published studies on FE were found...
Tipo: Text Palavras-chave: Feed conversion ratio; Feed efficiency; Feed intake; Fish; Genetics; Selection.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00415/52675/53771.pdf
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Apport d'un programme de génétique à une filière de production aquacole : l'exemple de l'ostréiculture ArchiMer
Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre.
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between...
Tipo: Text Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf
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Fecundity, growth rate and survivorship at the interface between two contiguous genetically distinct groups of Semibalanus balanoides ArchiMer
Brind'Amour, Anik; Bourget, Edwin; Tremblay, Rejean.
On the western coast of the Atlantic, according to the literature, 2 distinct groups of Semibalanus balanoides occur with a distinct interface near the Miramichi Estuary, New Brunswick, in the Gulf of St. Lawrence. On each side of this interface, the groups are characterized by clinal variations for MPI (mannose-6-phosphate isomerase) and GPI (glucose-6-phosphate isomerase). The present study was carried out to determine whether selection occurs at this interface, to establish how early in the sessile life period it occurs and to examine the selecting forces involved. Reciprocal transplant experiments of newly settled individuals to both sides of the interface were carried out. No significant differences specifically linked to source or destination were...
Tipo: Text Palavras-chave: Semibalanus; Genetics; Selection; Fecundity; Growth rate; Survival.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00048/15970/13410.pdf
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Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (Poster) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Early-life history of oysters, like in most marine bivalves, is characterized by high fecundity and low survival rate. In hatcheries, which are ecoming more and more important for the production of juveniles for aquaculture production, rearing conditions are optimised (density, feeding, temperature...), and contrast with those observed in the wild. Additionally, slow growing larvae are culled by sieving.
Tipo: Text Palavras-chave: Crassostrea gigas; Juvenile; Genetic variability; Selection; Genetic; Hatcheries; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3444.pdf
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Genetic improvement of fish: Achievements and challenges for tomorrow ArchiMer
Vandeputte, Marc; Baroiller, Jean-francois; Haffray, Pierrick; Quillet, Edwige.
Genetic improvement is a major contributing factor in developing efficient aquaculture, as it is in other forms of animal production. Moreover, as most fish species are only slightly domesticated, the potential gains are very large. Many of the current methods, developed during the XXth century, are classically used in livestock rearing (choice of breeds, crossbreeding, selective breeding). Others are much more specific to aquatic organisms (genetic control of sex, sterilization through triploidization). The spread of selected lines in aquaculture has been effective only since the 1990s, with lines selected essentially on growth and producing some impressive results. Transgenesis, although technically feasible on growth rate, is not used in practice and...
Tipo: Text Palavras-chave: Selection; Genomics; Fishes; Breeding; Aquaculture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6564.pdf
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