|
|
|
| Registros recuperados: 65 | |
|
|
| Neumann,Giovano; Bernardes Júnior,Jurandir Joaquim; Sanches,Paulo Vanderlei; Piana,Pitágoras Augusto; Bombardelli,Robie Allan. |
| ABSTRACT By using informetric tools, we evaluated the contribution and impact of the open source computer-assisted sperm analysis (CASA) software on sperm studies, especially pertaining to fish. The article was cited 125 times between 2007 and 2014 in four publication types (articles, doctoral dissertations, master’s theses, and books). Among articles, the number of citations increased at rate of three citations per year and the calculated h-index was 17. Of the studies that cited Wilson-Leedy and Ingermann (2007), fish were the main group of organisms studied (61 articles); of these, 34 articles used the open source CASA, covering 23 marine or freshwater species. Studies conducted in 12 countries were published in 21 journals. Four ways of knowledge... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Computational analysis; Fish; Motility; Scientometry; Spermatozoa. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000700560 |
| |
|
|
| Barros,Maurício Hoshino da Costa; Shiomi,Hugo Hideki; Amorim,Lincoln da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Siqueira,Jeanne Broch; Guimarães,José Domingos. |
| The objective of this study was to verify the effect of three protocols of cryopreservation on the sperm viability post-thawing of swine from the Piau breed (Sus scrofa), by means of evaluation assays in vitro and in vivo. Twenty-two ejaculates of 5 adult males of the Piau breed trough the gloved-hand method. In order to verify the sperm viability of the raw semen and post-thawing, the following traits were evaluated: sperm motility and vigor, hypoosmotic test, supra-vital color test and percentage of normal acrosomes. To freezing, the ejaculates were fractioned and submitted in: method 1, method of freezing recommended by Fürst et al. (2005), modified according to media extender; method 2, recommended by Fürst et al. (2005), modified according to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Boars; Intra-uterine insemination; Spermatozoa. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000400012 |
| |
|
| |
|
| |
|
| |
|
|
| Eiras-Stofella,Daura Regina; Gremski,Waldemiro; Kuligowski,Sandra Maria. |
| The structure of the spermatozoon of Mugil curema Valenciennes, 1836 was studied using scanning and transmission electron microscopy. The spermatic head is rounded and formed by the nucleus containing granular chromatin, firmly packed resulting in a mass extremely electron dense. The acrossome is absent. The midpiece is characterized by the presence of two centrioles, a plasmatic canal, very few vesicles, and several mitochondria (9-10) with aproximately 0.50µm in diameter. The head and the midpiece are aproximately 1.56µm in diameter. The flagellum conforms to the 9 + 0 flagellar pattern near the transition region in its midpiece and is 9 + 2 from there on up to the distal region of the axoneme. The electron density in the A tubules 1, 2, 5 and 6 shows... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Teleostei; Mugilidae; Mullet; Mugil curema; Ultrastructure; Spermatozoa. |
| Ano: 1993 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751993000400007 |
| |
|
| |
|
| |
|
| |
|
|
| Magnotti, Caio Cesar França; Castro, Jorgélia de Jesus Pinto; Pedrotti, Fabiola Santiago; Sterzelecki, Fábio Carneiro; Sanches, Eduardo Gomes; Cerqueira, Vinicius Ronzani. |
| The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.06.03.04-3 gamete conservation; Spermatozoa; Aquaculture; Marine fish farming; Near threatened fish.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563 |
| |
|
|
| Silva, Ricardo Tomaz da; Faculdade de Medicina Veterinária, Universidade Professor Antônio Carlos, Campus Uberlândia; Mendes Júnior, José Olavo Borges; Instituto de Estudos Avançados José Caetano Borges, Universidade de Uberaba, Uberaba; Beletti, Marcelo Emílio; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia. |
| O objetivo deste trabalho foi avaliar a relação entre a morfometria da cabeça do espermatozoide e a compactação da cromatina com as taxas de clivagem e formação de blastocisto em programas de produção de embriões in vitro (PIV). Neste estudo foram utilizados dados de 38 rotinas de PIVs com sêmen de touros zebuínos com espermograma de rotina normal, realizadas em dois diferentes laboratórios de Uberaba, Minas Gerais, Brasil. Para avaliar a morfometria e compactação da cromatina, foram usados esfregaços de sêmen corados com azul de toluidina e avaliados por análise de imagem computacional. A cromatina foi avaliada quanto à intensidade de compactação e homogeneidade. Os resultados permitem concluir que pequenas variações na morfometria da cabeça e na... |
| Tipo: Análise laboratorial |
Palavras-chave: 5.05.04.00-2 Reprodução Animal azul de toluidina; Bovinos; Cromatina; Espermatozóides; Fertilização in vitro. Reprodução Animal toluidine blue; Bovines; Chromatin; Spermatozoa; In vitro fertilization.. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/1002 |
| |
|
|
| Tenório, Bruno Mendes; Universidade Federal Rural de Pernambuco; Jimenez, George Chaves; UFRPE; Guerra, Maria Madalena Pessoa; UFRPE; Nogueira, Romildo de Albuquerque; UFRPE; Silva Junior, Valdemiro Amaro da; UFRPE. |
| A exposição da sociedade a Campos Eletromagnéticos (CEM) vem aumentando vertiginosamente em virtude da ampla expansão tecnológica observada nos últimos anos. Tanto a geração, como a distribuição e a utilização de energia elétrica podem gerar Campos Eletromagnéticos de baixa freqüência (50 e 60 Hz). Pesquisas vêm demonstrando que a exposição a estes CEM podem proporcionar alterações fisiológicas significativas, apesar disto, ainda não estão totalmente esclarecidos a extensão destes efeitos, nem os mecanismos de ação que envolve a interação dos CEM com os organismos biológicos. O presente trabalho teve como principal objetivo verificar os efeitos dos CEM (60 Hz e 1 mT) sobre a integridade de DNA e morfologia espermática de ratos sexualmente maduros, que... |
| Tipo: Estudos Morfológicos; Itegridade de DNA |
Palavras-chave: 2.06.00.00-3; 2.07.00.00-8 espermatozoide; Lesões de DNA; Patologias espermáticas Morfologia; Fisiologia electromagnetic wave; Non-ionizing radiation; Spermatozoa; DNA damage; Sperm pathologies. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5417 |
| |
|
|
| Gheller,S.M.M.; Corcini,C.D.; Santos,F.C.C.; Tavares,G.C.; Costa,V.G.G.; Curcio,B.R.; Nogueira,C.E.W.; Varela Junior,A.S.. |
| ABSTRACT This study was designed to evaluate the possible benefits of adding xanthan gum to a standard extender for equine through in vitro analyzes of sperm quality. Semen was collected four times from five different stallions (n= 20 samples) and subjected to cooled storage under different conditions: control (only standard extender) and three different concentrations of xanthan gum (0.01%, 0.12%, and 0.25%) supplemented to the extenders. Sperm parameters, such as motility, mitochondrial functionality, and membrane, acrosome, and DNA integrity were measured after 0h, 24h, 48h, and 72h of sperm storage at 5ºC. Our observations indicated that sperm motility declined with longer cooling period with the 0.25% xanthan gum supplementation group compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cooled; Stallion; Semen; Spermatozoa; Storage. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100028 |
| |
|
|
| Paschoal,A.F.L.; Santos,J.T.; Mellagi,A.P.G.; Bernardi,M.L.; Wentz,I.; Bortolozzo,F.P.. |
| ABSTRACT This study aimed to evaluate the use of some testicular traits to identify boars with low sperm morphological quality. The consistency (scores from 1 to 5), tone with tonometry (mm), parenchyma echogenicity and heterogeneity with ultrasound (pixel) were assessed in 402 mature boars (18.5 months on average). Sperm abnormality thresholds (≤ 25% of total sperm abnormalities, ≤ 5% of heads, acrosome, neck or midpiece defects, ≤ 10% tail defects, and 15% cytoplasmic droplets) were used to classify boars as approved or disapproved. Three classes of testicular traits were formed (extremely low and high values, approximately 15% each, were kept in separated classes). When the traits were individually evaluated, fewer boars were approved if the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Andrology; Breeding soundness; Consistency; Echotexture; Morphology; Spermatozoa. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401277 |
| |
|
| |
|
|
| Pinto,S.C.C.; Almeida,D.S.; Alves,M.B.R.; Florez-Rodriguez,S.A.; Abreu Júnior,G.S.; Alves,N. Britto e; Celeghini,E.C.C.; Laskoki,L.M.; Souza,F.A.. |
| ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; Sperm membranes; Spermatozoa. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100009 |
| |
|
|
| Aguiar,G.V.; van Tilburg,M.F.; Catunda,A.G.V.; Celes,C.K.S.; Lima,I.C.S.; Campos,A.C.N.; Moura,A.A.A.; Araújo,A.A.. |
| The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity) were analyzed. It was observed a reduction (P<0.05) in normal sperm morphology, fructose, citric acid, P, Mg and total protein concentration during the dry season, which did not affect the motility, vigor, volume and sperm concentration. Phospholipase A2 activity was increased during the dry season (P<0.05). The analysis of the semen cooled at 4ºC during 48 hours showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen; Spermatozoa; Season; Cooling; Caprine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100002 |
| |
|
|
| Orlandi Introíni,Gisele; Martins Maester,Fernanda; Pedini Pereira Leit,Fosca; Recco-Pimentel,Shirlei Maria. |
| Ultrastructural analyses of bivalve spermatozoa are relevant in studies that aim to identify taxonomic traits for the purposes of discriminating species and conducting phylogenetic studies. In the present work, spermatozoa of mussel specimens of the genus Mytella, collected from two populations living in distinct habitats, were examined by electron microscopy. The objective was to identify sperm ultrastructural taxonomic traits that could be used to differentiate Mytella species. The specimens were from populations that live in intertidal zones on the southeast coast of Brazil, either buried in muddy-sand sediment or anchored to rocky substrates. The acrosomal vesicle was conical and long, the axial rod extended from the nucleus to the acrosome, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mytilidae; Spermatozoa; Electron microscopy; Mussel. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300002 |
| |
|
|
| Soon-Jeong,Jeong; Joo-Cheol,Park; Heung-Joong,Kim; Chun,Sik Bae; Myung-Hee,Yoon; Do-Seon,Lim; Moon-Jin,Jeong. |
| This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew ( Crocidura suaveolens ), the Japanese white-toothed shrew ( C. dsinezumi ) and the big white-toothed shrew ( C. lasiura ) belonging to the subfamily Crocidurinae living in Korea . In the spermatozoa of C. suaveolens , the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura , the head has a large acrosome, a serrated inner acrosomal membrane and a common apical body.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crocidurinae; Shrew; Spermatozoa; Phylogeny; Fine structure. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200003 |
| |
|
| |
| Registros recuperados: 65 | |
|
|
|
