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Interactions entre traits de personnalité des téléostéens, environnement et polluants ArchiMer
Alfonso, Sebastien.
In the frame of this thesis, we first studied the establishment of personality in teleost, the consistency across context and different life stages as well as the associated physiological mechanisms. Personality appeared consistent across contexts over a short time period but it was no longer the case over a long period of time, especially between different life stages. Physiological mechanisms of stress regulation, as well as activity and neural plasticity, differed according to individual boldness. Then, we studied the physiological and behavioural responses of teleost fish chronically exposed to an environmentally relevant mixture of POP (PCBs and PBDEs), as well as those of their unexposed offspring. Different behavioural alterations in larval...
Tipo: Text Palavras-chave: Polluants; Comportement; Environnement; Stress; Polluants; Stress; Teleost fish; Environment.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00499/61019/64421.pdf
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Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage ArchiMer
Mugnier, Chantal; Soyez, Claude.
The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied....
Tipo: Text Palavras-chave: Temperature; Stress; Penaeid shrimp; Osmoregulation; Molt stage; Litopenaeus stylirostris; Hypoxia.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-372.pdf
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Spatial distribution and activity patterns as welfare indicators in response to water quality changes in European sea bass, Dicentrarchus labrax ArchiMer
Alfonso, Sebastien; Sadoul, Bastien; Cousin, Xavier; Bégout, Marie-laure.
In aquaculture, fish are exposed to unavoidable stressors that can be detrimental for their health and welfare. However, welfare in farmed fish can be difficult to assess, and, so far, no standardized test has been universally accepted as a welfare indicator. This work contributes to the establishment of behavioural welfare indicators in a marine teleost in response to different water quality acute stressors. Groups of ten fish were exposed to high Total Ammonia Nitrogen concentration (High TAN, 18 mg.L-1), Hyperoxia (200 % O2 saturation), Hypoxia (20 % O2 saturation), or control water quality (100% O2 saturation and TAN < 2.5 mg.L-1) over 1 hour. Fish were then transferred in a novel environment for a group behaviour test under the same water quality...
Tipo: Text Palavras-chave: Fish; Welfare; Water quality; Behaviour; Stress.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00612/72399/71344.pdf
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Effects of rearing density on sea bass (Dicentrarchus labrax) biological performance, blood parameters and disease resistance in a flow through system ArchiMer
Roque D'Orbcastel, Emmanuelle; Lemarie, Gilles; Breuil, Gilles; Petochi, Tommaso; Marino, Giovanna; Triplet, Sebastien; Dutto, Gilbert; Fivelstad, Sveinung; Coeurdacier, Jean-luc; Blancheton, Jean-paul.
During 84 days, the effects of density on juvenile sea bass (Dicentrarchus labrax) (76 +/- 16 g) were studied in an experimental tank-based flow through system. Performance, stress response and resistance to virus infection were analysed under five stabilized rearing densities: 10, 20, 40, 70 and 100 kg m(-3). Water quality parameters (CO2, total ammonia nitrogen and O-2) were measured and maintained close to the recommended values for farmed sea bass by adjusting water renewal exchange. No significant differences were observed between density treatments, neither on stress response (cortisol) nor susceptibility to nodavirus. With regards to biological performances, the daily feed intake and specific growth rate were significantly lower in fish reared at...
Tipo: Text Palavras-chave: Rearing density; Flow through system; Stress; Water quality; Sea bass.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11287/7824.pdf
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Measuring cortisol, the major stress hormone in fishes ArchiMer
Sadoul, Bastien; Geffroy, Benjamin.
Stress in teleosts is an increasingly studied topic because of its interaction with growth, reproduction, immune system and ultimately fitness of the animal. Whether it is for evaluating welfare in aquaculture, adaptive capacities in fish ecology, or to investigate effects of human‐induced rapid environmental change, new experimental methods to describe stress physiology in captive or wild fish have flourished. Cortisol has proven to be a reliable indicator of stress and is considered the major stress hormone. Initially principally measured in blood, cortisol measurement methods are now evolving towards lower invasiveness and to allow repeated measurements over time. We present an overview of recent achievements in the field of cortisol measurement in...
Tipo: Text Palavras-chave: Aquaculture; Cortisol; Glucocorticoids; Non-invasive; Stress; Welfare.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00478/59001/61616.pdf
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Mise au point d'une méthode standardisée de stress chez Crassostrea gigas ArchiMer
Glemarec, Yves-marie; Haure, Joel; Baud, Jean-pierre.
Cette étude a pour objectif de mettre au point un test standardisé de choc thermique applicable sur le naissain d'huître creuse Crassostrea gigas, facile à mettre en oeuvre, rapide et de faible coût. Ce test doit permettre d'envisager à terme un transfert de cette technique à la profession ostréicole. Dans un premier temps, un modèle a été validé afin de prendre en compte le poids moyen, vis à vis du temps d'application du choc thermique en eau douce à 45°C appliqué aux huîtres pour obtenir 50% de mortalité. Il permet de dresser une table de correspondance poids des juvéniles, temps d'application du stress pour une gamme de 10 à 500mg. La méthodologie retenue pour le stress standard peut être résumée ainsi : - Tamisage d'une population de jeunes huîtres en...
Tipo: Text Palavras-chave: Crassostrea gigas; Stress; Choc thermique; Mortalité.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00304/41543/40751.pdf
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Disturbance induced decoupling between host genetics and composition of the associated microbiome ArchiMer
Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in...
Tipo: Text Palavras-chave: Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28202/26430.pdf
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Energetic adjustments in freely breeding-fasting king penguins: does colony density matter? ArchiMer
Viblanc, Vincent A.; Saraux, Claire; Malosse, Nelly; Groscolas, Rene.
1. For seabirds that forage at sea but breed while fasting on land, successful reproduction depends on the effective management of energy stores. Additionally, breeding often means aggregating in dense colonies where social stress may affect energy budgets. 2. Male king penguins (Aptenodytes patagonicus) fast for remarkably long periods (up to 1 center dot 5months) while courting and incubating ashore. Although their fasting capacities have been well investigated in captivity, we still know very little about the energetics of freely breeding birds. 3. We monitored heart rate (HR, a proxy to energy expenditure), body temperature and physical activity of male king penguins during their courtship and first incubation shift in a colony of some 24000 freely...
Tipo: Text Palavras-chave: Social density; Body temperature; Seabird; Fasting; Stress; Physical activity; Heart rate; Energy expenditure.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00193/30447/29090.pdf
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GigaTON: an extensive publicly searchable database providing a new reference transcriptome in the pacific oyster Crassostrea gigas ArchiMer
Riviere, Guillaume; Klopp, Christophe; Ibouniyamine, Nabihoudine; Huvet, Arnaud; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly...
Tipo: Text Palavras-chave: Oyster; Transcriptome; Development; Stress; Tissues; NGS.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00302/41321/40520.pdf
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Summer mortality of the Pacific cupped oyster Crassostrea gigas in the Bay of Marennes-Oleron (France) ArchiMer
Goulletquer, Philippe; Soletchnik, Patrick; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Faury, Nicole.
A comprehensive research program has been developed since 1995 to focus on C. gigas abnormal mortality rates occurring regularly during the summer period in the Bay of Marennes Oleron (France). Several subprojects were carried out including in-vitro ecophysiological studies to asses l) the relationship between reproductive status, scope for growth and mortality, 2) effects of stress temperature on physiological functions. Meanwhile, field studies concerned monitoring surveys of calibrated oyster populations at various emersion time and geographic sites. A database was developed and incorporated into a geographic information system (GIS) to overlay several layers of information and establish relationship among environmental parameters and mortality rates....
Tipo: Text Palavras-chave: Marennes Oleron bay; In situ continuous monitoring; Ecophysiology; Stress; Environmental conditions; Summer mortality; Crassostrea gigas; Marennes Oléron; In situ en continu; Suivis environnementaux; Ecophysiologie; Stress; Conditions environnementales; Mortalité estivale; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-3093.pdf
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Combined effect of external ammonia and molt stage on the blue shrimp Litopenaeus stylirostris physiological response ArchiMer
Mugnier, Chantal; Justou, Carole.
The effect of ambient ammonia and molt stage on the physiological response of the blue shrimp Litopenaeus stylirostris was studied. Shrimps were submitted to 54.6 mg l(-1) ammonia-N (1.76 mg l(-1) NH3-N) for 24 h. Only shrimps in stages C, D-0, D-1 and D-2 were used for the analysis. Haemolymph was assayed for osmoregulatory capacity (OC), magnesium ion (Mg ion), total proteins, oxyhaemocyanin, lactate and glucose. Molt stage had an effect on OC, Mg ion and total proteins in control shrimps, and on OC, Mg ion and lactate in treated animals. Ammonia treatment decreased OC and lactate, and increase Mg ion concentration in haemolymph, for all molt stages. It decreased significantly total proteins and oxyhaemocyanin for stages D-1 and D-2, and increased...
Tipo: Text Palavras-chave: Stress; Peneid shrimp; Molt stage; Litopenaus stylirostris; Haemolymph constituents; Ammonia.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-371.pdf
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Le stress du passage en mer de post-larves de coquilles Saint-Jacques (Pecten maximus) issues d'écloserie ArchiMer
Philippe, Régis.
Le transfert en mer de post-larves de coquille Saint-Jacques depuis la nurserie, à terre, jusqu'à la station de prégrossissement, en mer, provoque de faibles survies : 20 à 50 % L'étude du passage en mer montre 2 stress élémentaires principaux : la dé-fixation des post-larves et leur émersion pendant le transport (l'effet des chocs thermiques n'a pas été étudié ici). Quelques comparaisons de lots de post-larves dé-fixées ou émergées, montrent que la cause principale de mortalité serait la dé-fixation. La dernière partie de cette étude aborde des essais de transfert sans dé-fixation, à l'aide de supports-collecteurs immergés pendant l'élevage post-larvaire. Les survies au passage en mer apparaissent beaucoup plus satisfaisantes (64 à 100 % dans la plupart...
Tipo: Text Palavras-chave: Coquille Saint-Jacques; Pecten maximus; Naissain; Passage en mer; Stress; Dé-fixation; Emersion; Collecteurs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00422/53377/54221.pdf
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Optimisation d'une méthode standardisée de choc thermique sur des juvéniles de Crassostrea gigas : application à des populations d'ecloseries ArchiMer
Catois, Stéphane; Haure, Joel; Martin, J-l.; Nourry, Max; Palvadeau, Hubert; Baud, Jean-pierre.
Mortality among juvenile Crassostrea gigas has been occuring during the summer months since 1995 and is now considered to be a real problem for French oyster culture. The aim of this study was to standardise the stress that could be applied to Crassostrea gigas spat. In initial experiments in 1998, the weight of individual spat and the duration of a heat shock at 50°C in freshwater, adapted for oysters of between 500 and 2000 mg, were built into a model to obtain 50% mortality. However, the precision was low (50%) and will need to be improved in the future. Following this, hatchery-produced juveniles weighing 10 to 500 mg were subjected to heat shock at 45°C in heshwater ; the duration of the shock was calculated based on a model built in 1997. The results...
Tipo: Text Palavras-chave: Crassostrea gigas; Stress; Choc thermique; Mortalité; Crassostrea gigas; Stress; Heat shock; Mortality.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00080/19095/16690.pdf
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Enhanced brain expression of genes related to cell proliferation and neural differentiation is associated with cortisol receptor expression in fishes ArchiMer
Sadoul, Bastien; Alfonso, Sebastien; Bessa, E.; Bouchareb, A.; Blondeau-bidet, E.; Clair, P.; Chatain, Beatrice; Begout, Marie-laure; Geffroy, Benjamin.
Stress enhances or inhibits neurogenesis in mammals and some fish species. The link between the two processes is still unclear. Most studies have been performed in very specific stressful or altered environments. Despite the known inter-individual divergence in coping abilities within populations, the relationship between the stress axis and neurogenesis has never been addressed in unstressed individuals. Here we correlate brain expression of the pcna (proliferating cell nuclear antigen) and neurod1 (neurogenic differentiation factor 1) genes, two markers of neurogenesis, with transcripts of cortisol receptors in three fish species living in very distinct environments. Within the three species, individuals with the highest expression of neurogenesis genes...
Tipo: Text Palavras-chave: Neurogenesis; Stress; Glucocorticoid receptor; Mineralocorticoid receptor; Hypothalamo-pituitary-interrenal axis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00444/55560/57164.pdf
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Spatial and temporal variation of three biomarkers in Mytilus edulis ArchiMer
Strubbia, Sofia; Lyons, B. P.; Lee, R. J..
Environmental conditions and xenobiotic exposure can be sources of stress to living organisms. Biological markers are measurable indicator of changes which may happen at any biological level and which can be considered an early warning signal of some biological or environmental state or condition. A structured field study was undertaken to investigate the relationship between three biomarker assays and the spatial and temporal variation of each biomarker in samples of Mytilus edulis. The three biomarkers were the neutral red retention assay, micronucleus assay and comet assay, which indicate damage at different cellular/molecular levels. Three sites in Poole Harbour, an area on the South coast of the UK were sampled on six separate occasions at least three...
Tipo: Text Palavras-chave: Mytilus edulis; Biomarkers; Stress; Neutral red retention; Comet assay; Micronucleus assay; Poole Harbour.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00481/59274/63447.pdf
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Bivalve genomics ArchiMer
Saavedra, Carlos; Bachere, Evelyne.
Interest in bivalve genomics has emerged during the last decade, owing to the importance of these organisms in aquaculture and fisheries and to their role in marine environmental science. Knowledge of bivalve genome structure, function and evolution resulting from 20th century "single gene" approaches is limited, but genomic technologies are called to dramatically increase it. Research based on linkage maps, transcriptomics and proteomics is being carried out to study the genetic and molecular bases of traits of interest in bivalve farming industry, mainly disease susceptibility, tolerance to environmental stress, and growth. The Pacific oyster (Crassostrea gigas) is now the focus of an international genome-sequencing consortium. The use of bivalves in...
Tipo: Text Palavras-chave: Shellfish toxins; Feeding; Stress; Proteomics; Genomics; Bivalves.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1709.pdf
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Mortalité estivale es huîtres (C. gigas) : questions souvent posées et éléments de réponse ArchiMer
Soletchnik, Patrick.
Comment peuvent se comprendre et s'expliquer les mortalité estivales de l'huître creuse en Charentes Maritimes? Sur la base des travaux effectués au cours du défi MOREST (2002-2005), ce poster présente un schéma conceptuel du processus de mortalités estivales, et illustre à partir de dessins humoristiques, les différents stress qui peuvent provoquer des mortalités sur des huîtres fragilisées par des conditions automnales et hivernales défavorables.
Tipo: Text Palavras-chave: Stress; Mortalités estivales; Crassostrea gigas; Huîtres.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4037.pdf
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Response of Listeria monocytogenes to liquid smoke ArchiMer
Guilbaud, Morgan; Chafsey, Ingrid; Pilet, Marie-france; Leroi, Francoise; Prevost, Herve; Hebraud, Michel; Dousset, Xavier.
Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes. Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly...
Tipo: Text Palavras-chave: Survival; Stress; Proteome analysis; Listeria monocytogenes; Liquid smoke; Haemolysis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4522.pdf
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Prenatal Water Deprivation Induces Apoptosis in Sexual Dimorphic Nucleus of the Brain of Male New Born Sprague-Dawley Rats International Journal of Morphology
Chehreie,Shima; Sadri,Soheil; Khazaei,Mozafar; Ghanbari,Ali; Ayubian,Mahmood; Amiri,Sara.
Considering the size of some nuclei and area, sex hormones control the sexual development of the brain. The sexual development of the brain can also be influenced by environmental stress. This study aimed to clear the effect of prenatal water deprivation on the development of sexual dimorphic nucleus (SDN) of the brain. In this research, pregnant rats were divided into two groups (control and treated). For the treated animals, water was removed from the ewes for 48 h at the end of third trimester of gestation (19-21 days). TUNEL staining was used for detection of apoptosis in paraffin embedded diencephalon selected sections. The ratio of apoptotic cells to non- apoptotic ones was calculated as apoptotic index. Differences of apoptotic index and serum...
Tipo: Journal article Palavras-chave: Apoptosis; Rat; SDN nucleus; Stress; TUNEL.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200032
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Association between handling stress in the corral and rabies antibody titers in selenium-supplemented cattle J. Venom. Anim. Toxins incl. Trop. Dis.
Reis,LSLS; Chiacchio,SB; Oba,E; Pardo,PE; Frazatti-Gallina,NM.
This study determined the correlation between serum cortisol levels and rabies antibody titers in cattle primo-vaccinated against rabies and supplemented with dietary selenium (Se). Sixty Nelore male calves (10 to 12 months old) received daily and individual dietary supplementation with 0, 3.6, 5.4 and 6.4 mg Se (groups Gc, G3.6, G5.4 and G6.4, respectively). The animals were vaccinated against rabies (day 0) and subjected to handling stress in the corral for 120 days. Blood sampling procedures were performed on days 0, 15, 30, 60, 90 and 120. Cortisol levels increased until day 90, but had dropped significantly by day 120 (P < 0.01). Rabies antibody titers on days 30 and 90 were similar among Se-supplemented groups; in the control group, rabies...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cattle; Cortisol; Stress; Rabies immune response; Selenium supplementation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400015
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