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Registros recuperados: 75
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Application of the taguchi’s loss function in the breeding of nile tilapia fed with different levels of alcohol yeast BABT
Medri,Vandir; Pereira,Geni Varéa; Leonhardt,Júlio Hermann.
Studies were conducted to evaluate the effect of 10%, 20% and 30% ration substitutions for distillery yeast on the function Taguchi’s loss using two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g placed in amianthus box.. The average results obtained for the limnological parameters of the water quality control through chemical analysis were considered normal. Taguchi’s losses for the total production, for the dead and discarded fishes did not show correlation for the different levels of alcohol yeast inclusion, showing that the choice of the yeast level in the ration for these fishes depended on its availability and occasional cost. It was observed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quality; Ration; Taguchi; Tilapia; Yeast.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100011
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Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation BABT
Araújo,Lúcia de Fátima; Medeiros,Ariosvaldo Nunes; Perazzo Neto,Américo; Oliveira,Líbia de Sousa Conrado; Silva,Flávio Luiz Honorato da.
The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein enrichment; Solid-state fermentation; Cactus pear; Yeast.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400020
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Growth of Nile tilapia Oreochromis niloticus fed with different levels of alcohol yeast BJB
MEDRI,V.; PEREIRA,G. V.; LEONHARDT,J. H..
Two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g, distributed in a totally casualized delineation, during 330 days. The effect of the substitution of 10%, 20% and 30% of the ration by distillery yeast (Saccharomiyces cerevisae) in balanced experimental rations on the development in the breeding of tilapias did not show a harmful effect up to the maximum tested level of 30%, showing that the choice of the yeast in the ration for these fishes depends on the availability and occasional cost. The yeast provides fish with good resistance to unfavorable environmental conditions, which could be verified since there were no statistical differences among the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Oreochromis niloticus; Ration; Tilapia.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000100014
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Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids BJMBR
Schmidt,M.; Cömer,A.; Grey,M.; Brendel,M..
Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO2 during fermentation, i.e., the selecting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Transformation; Hyperresistance to formaldehyde.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200004
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Shared control of maltose and trehalose utilization in Candida utilis BJMBR
Rolim,M.F.; de Araujo,P.S.; Panek,A.D.; Paschoalin,V.M.F.; Silva,J.T..
Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 µmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trehalose; Trehalase; Sugar transporter; Candida utilis; Yeast.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700002
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Yeast CUP1 protects HeLa cells against copper-induced stress BJMBR
Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C..
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616
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The biological effects of high-pressure gas on the yeast transcriptome BJMBR
Matsuoka,H.; Suzuki,Y.; Iwahashi,H.; Arao,T.; Suzuki,Y.; Tamura,K..
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA microarray; Yeast; Hydrostatic pressure; Gas pressure; Nitrogen gas.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800016
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Interdigital foot infections: Corynebacterium minutissimum and agents of superficial mycoses BJM
Sariguzel,Fatma Mutlu; Nedret Koc,A.; Yagmur,Gülhan; Berk,Elife.
Interdigital foot infections are mostly caused initially by dermatophytes, yeasts and less frequently by bacteria. Erythrasma caused by Corynebacterium minutissimum can be confused with superficial mycoses. The aim of the study was to determine the prevalence of the etiologic agents of superficial mycoses and the frequency of Corynebacterium minutissimum in interdigital foot infections. All the samples obtained from the 121 patients with interdigital foot infections were examined directly with the use of 20% potassium hydroxide mounts and Gram stain under the microscope and cultured on Sabouraud's dextrose agar plates. In identification of superficial mycoses, the rate was found to be 14% with the cultural method and 14% with direct microscopic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Interdigital foot infections; Superficial mycoses; Dermatophytosis; Yeast; Corynebacterium minutissimum.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300003
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Development and characterization of hybrids from native wine yeasts BJM
García,Verónica; Rivera,José; Contreras,Angela; Ganga,María Angélica; Martínez,Claudio.
For commercial purposes, the winemaking industry is constantly searching for new yeast strains. Historically, this has been achieved by collecting wild strains and selecting the best for industrial use through an enological evaluation. Furthermore, the increasing consumer demands have forced the industry to incorporate new strategies such as genetic engineering to obtain improved strains. In response to the lack of public acceptance of this methodology, alternative strategies based on breeding have gained acceptance in recent years. Through the use of conjugation of individual spores without the support of genetic engineering methods we generated intraspecific hybrids from wild strains with outstanding enological characteristics and interdelta...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Hybrids; Wine.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200008
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Analysis of the secondary compounds produced by Saccharomyces cerevisiae and wild yeast strains during the production of "cachaça" BJM
Dato,Maria Cecília Fachine; Pizauro Júnior,João Martins; Mutton,Márcia Justino Rossini.
The aim of this study is to compare the composition of "cachaças" produced in 10 fermentation cycles by Saccharomyces cerevisiae (Sc) and wild yeast strains [Pichia silvicola (Ps), Pichia anomala 1 (Pa1), Pichia anomala 2 (Pa2) and Dekkera bruxelensis (Db)], isolated from distilleries in Jaboticabal - SP, Brazil. The secondary components of the heart fraction were determined by gas chromatography. The levels of secondary components were influenced by the wine pH, which varied among yeast strains. S. cerevisiae showed slightly more secondary components, whereas wild strains produced more higher alcohols. Wild yeast strains were shown to be adequate for the production of a high quality "cachaça".
Tipo: Info:eu-repo/semantics/article Palavras-chave: Distillate composition; Aldehyde; Higher alcohols; Secondary components; Yeast.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100014
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Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate BJM
Accorsini,Fábio Raphael; Mutton,Márcia Justino Rossini; Lemos,Eliana Gertrudes Macedo; Benincasa,Maria.
Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Biosurfactants; Glycerol; Soybean oil.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100013
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Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris BJM
Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G..
ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid peroxidation; Pyruvate pathway; Yeast.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400856
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Antifungal activity of lectins against yeast of vaginal secretion BJM
Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
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Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa BJM
Maldonade,Iriani R.; Rodriguez-Amaya,Delia B.; Scamparini,Adilma R. P..
Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2(5-1) experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD) to optimise carotenoid production, which was adequately approximated with a full quadratic equation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Torulene; Yeast; Response surface design; Rhodotorula mucilaginosa.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100012
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Ethanol production in Brazil: a bridge between science and industry BJM
Lopes,Mario Lucio; Paulillo,Silene Cristina de Lima; Godoy,Alexandre; Cherubin,Rudimar Antonio; Lorenzi,Marcel Salmeron; Giometti,Fernando Henrique Carvalho; Bernardino,Claudemir Domingos; Amorim Neto,Henrique Berbert de; Amorim,Henrique Vianna de.
ABSTRACT In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Yeast; Fermentation; Distillery.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500064
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Use of Pseudomonas stutzeri and Candida utilis in the improvement of the conditions of Artemia culture and protection against pathogens BJM
Abdelkarim,Mahdhi; Kamel,Chaieb; Fathi,Kammoun; Amina,Bakhrouf.
To evaluate the effect of two bacterial strains isolated from Artemia cysts and yeast (Candida utilis) on the survival, growth and total biomass production of its larvae, challenge tests were performed with Candida utilis, Pseudomonas stutzeri and Pasteurella haemolityca. In addition, a pathogenic strain of Vibrio alginolyticus was tested for comparative purposes. Pseudomonas stutzeri and Candida utilis have no impact on survival, but enhance growth and total biomass production of the larvae. However, we noted that Pasteurella haemolityca affect negatively Artemia larvae. The adhesion and antagonism assay demonstrates that Candida utilis and Pseudomonas stutzeri are fairly adherent and play an important role in the enhancement of the protection of Artemia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Probiotics; Adhesion; Yeast; Bacteria.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100017
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Chlorine dioxide against bacteria and yeasts from the alcoholic fermentation BJM
Meneghin,Silvana Perissatto; Reis,Fabricia Cristina; Almeida,Paulo Garcia de; Ceccato-Antonini,Sandra Regina.
The ethanol production in Brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. Addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. This work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant bacteria (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides) from alcoholic fermentation, through the method of minimum inhibitory concentration (MIC), as well as its effect on the industrial yeast inoculum. Lower MIC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorine dioxide; Bacteria; Yeast; Antibacterial agent; Alcohol; Fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200026
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Mycological contamination in dental unit waterlines in Istanbul, Turkey BJM
Kadaifciler,Duygu Göksay; Ökten,Suzan; Sen,Burhan.
Studies on dental units (DUs) are conducted either for the prevention or the reduction of the density of bacterial contamination in dental unit waterlines (DUWLs). However, the existence of fungi in the these systems requires more attention. During dental treatment, direct contact with water contaminated with fungi such as Candida, Aspergillus, or inhalation of aerosols from high-speed drill may cause various respiratory infections, such as asthma, allergies, and wounds on mucose membranes, especially on immunocompromised patients and dentists. The aims of this study are to investigate the number and colonization of fungi in DUWLs in the city of Istanbul, Turkey. Water samples were collected from air-water syringes, high-speed drills, and inlet waters from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dental unit waterlines; Dental equipments; Filamentous fungi; Mycological contamination; Yeast.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300049
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Occurrence of killer Candida glabrata clinical isolates BJM
O,Arroyo-Helguera; Alejandro,De Las Penas; Irene,Castaño.
In this work we characterized the occurrence of killer activity in 64 Candida glabrata clinical isolates under different conditions. We found that only 6.25 % of the clinical isolates tested were positive for killer activity against a Saccharomyces cerevisiae W303 sensitive strain. Sensitivity of killer activity to different values of pH and temperatures was analyzed. We found that the killer activity presented by all isolates was resistant to every pH and temperature tested, although optimal activity was found at a range of pH values from 4 to 7 and at 37ºC. We did not observe extrachromosomal genetic elements associated with killer activity in any of the positive C. glabrata isolates. The killer effect was due to a decrease in viability and DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida glabrata; Killer activity; Yeast; Saccharomyces.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300005
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Hyphal-like extension and pseudohyphal formation in industrial strains of yeasts induced by isoamyl alcohol BJM
Ceccato-Antonini,Sandra Regina; Silva,Paula Cristina da.
Yeasts can produce pseudohyphae and hyphal-like extensions under certain growth conditions like isoamyl alcohol (IAA) induction, a chief constituent of fusel oil, which is a subproduct from the ethanolic fermentation. The morphology switch from yeast to a filamentous form can be troublesome to the process. In this work it was studied the influence of fusel alcohols, nitrogen sources (ammonium sulphate and leucine) and glifosate (a chemical maturator for sugar cane) added to a complex medium on some industrial strains of yeasts isolated from the fermentative process. Two industrial strains showed transition to hyphal-like extensions or pseudohyphae (clusters of cells) upon addition of IAA from 0.3 to 0.9% /v. The alterations were reversible when the yeasts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isoamyl alcohol; Ethanol; Yeast; Pseudohyphae; Hyphal-like extension.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300004
Registros recuperados: 75
Primeira ... 1234 ... Última
 

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