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| Registros recuperados: 159 | |
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| TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions... |
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Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838 |
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| TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽. |
| Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers. |
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Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868 |
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| 松本, 高太郎; 村上, 智亮; 菅生, 樹春; 山田, 一孝; 古林, 与志安; 松井, 高峯; 猪熊, 壽; MATSUMOTO, Kotaro; MURAKAMI, Tomotaka; SUGO, Kiharu; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; MATSUI, Takane; INOKUMA, Hisashi. |
| 17日齢のホルスタイン種雌子牛が，神経症状を示した. 病畜は頭部を左後方に折り曲げて伏臥姿勢を示し，時折自力で起立したがすぐに倒れ歩行不能であった. 神経学的検査では右の対光反射，威嚇瞬き反射，眼瞼反射， および音響耳介反射が消失していた. ビタミンB1，およびデキサメサゾンの投与により第7病日に歩行可能となったものの左への斜頸・旋回が認められ，神経学的検査所見に改善は認められなかった. 第7病日に病理解剖を行い，肉眼的には橋の断面右側に直径1cm大の膿瘍を認め，嫌気培養にてFusobacterium necrophorumが検出された. 左側への斜頸，聴覚の消失等の脳神経疾状は，橋の右側の膿瘍により内耳神経等の脳神経核が圧迫された結果出現した聴覚障害，平衡障害のためと考えられた. A 17-day-0ld female Holstein calf exhibited the following neurological symptoms: the calf bent its neck to the left，turning its face backward and could stand alone，but would fall within a short time. Neurological examination further revealed losses of pupillary light reflex， menace response，palpebral reflex，and hearing on the right side. After administration of dexamethasone and vitamin B1，the calf was able to walk... |
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Palavras-chave: 脳幹部膿瘍; 牛; Fusobacterium necrophorum; Brain stem abscess; Cattle. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2945 |
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| TAWFEEQ, Mohammad Monir; TAGAWA, Michihito; ITOH, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽. |
| A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression... |
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Palavras-chave: Biomarker; Bovine leukosis; Gene expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4096 |
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| 三浦, 沙織; 千葉, 史織; 高橋, 裕之; 福田, 茂夫; 藤井, 貴志; 新井, 鐘蔵; 古林, 与志安; 猪熊, 壽; MIURA, Saori; CHIBA, Shiori; TAKAHASHI, Hiroyuki; FUKUDA, Shigeo; FUJII, Takashi; ARAI, Shozo; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi. |
| 38カ月齢のホルスタイン種乳牛において，体全体が左側に傾くという神経症状が進行性に認められた．経過，左側対光反射と左側音響耳介反応の減弱，及び聴性脳幹誘発電位測定検査における左刺激時Ⅲ波-Ⅴ波の遅延所見より，延髄から橋における片側性圧迫性病変を疑った．病理解剖の結果，橋左側に帯黄色脆弱化病巣を認め，病理組織学的検索により，病変は腫瘍であり，迷入組織に由来する可能性があるものの，原発組織は確定できなかった． |
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Palavras-chave: 聴性脳幹誘発電位; 脳幹; 腫瘍; ホルスタイン. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4010 |
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| 猪熊, 壽; 横山, 直明; INOKUMA, Hisashi; YOKOYAMA, Naoaki. |
| 北海道東部(道東)の放牧地における牛の小型ピロプラズマ感染りスク低減要因について調査した。道東ではシュルツェマダニ，ヤマトマダニ，ダグラスチマダニが採取され， PCRにより小型ピロプラズマ遺伝子断片が検出され，これらのマダニがベクターとなりうる可能性が考えられた。エゾシカからは多数のマダニが回収され，小型ピロプラズマのベクターを牛の放牧地内へ持ち込むという意味で小型ピロプラズマ感染リスクに関わる動物と考えられた。また，放牧管理体制との関連では，野生動物侵入防止柵の設置，あるいは牧柵周辺の下草刈りが小型ピロプラズマ感染リスク低減に有効である可能性が考えられた。 Factors to decrease risk for Theileria orientalis infection of cattle in pastureland in Eastern Hokkaido were investigated. Ixodes persulcatus，Ixodes ovatus and Haemaphysalis douglusii were collected from the vegetation of the pastureland，and DNA fragments of T. orientalis were detected from these ticks by using PCR. The results suggested that these tick species may be vectors for T. orientalis in this area. Many ticks have been recovered from wild sika deer，and it was thought... |
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Palavras-chave: 道東; リスク低減要因; Theileria orientalis; Eastern Hokkaido; Factors to decrease risk. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2790 |
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| TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽. |
| The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower... |
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Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870 |
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| KOYAMA, Kenji; FUJITA, Riku; MAEZAWA, Masaki; FUKUMOTO, Natsuko; HORIUCHI, Noriyuki; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 猪熊, 壽; 古林, 与志安. |
| A 10-day-old, Japanese Black, female calf had shown astasia since just after birth. Focal symmetrical periventricular malacic lesions of the cerebrum and suppurative arthritis of the left hip joint were observed in macroscopic examination. Histologically, the cerebral lesions were confirmed as periventricular leukomalacia (PVL). The location and histological features of the lesions were similar to PVL in humans, caused by neonatal ischemia/hypovolemia. This is the first report of PVL in a neonatal calf. |
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Palavras-chave: Ischemia; Neonatal calf; Periventricular leukomalacia. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4324 |
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| Registros recuperados: 159 | |
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