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| Registros recuperados: 273 | |
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| De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
| Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
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| Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre. |
| Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée). |
| Tipo: Text |
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf |
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| Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre. |
| Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,... |
| Tipo: Text |
Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,... |
| Tipo: Text |
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf |
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| Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre. |
| Oysters, like many other marine bivalves, are characterized by high fertility and low survival rates during the precocious stages. Hatchery reproduction, realised in very different conditions than in their natural habitat (density, food, temperature, etc.), tends to optimize the production of larvae and juveniles. As a consequence, slow growing larvae are systematically culled. Previous results suggest the existence of a genetic variability linked to precocious development. Our approach consists in studying the evolutionary process (genetic drift and selection) linked with hatchery reproduction. Thanks to microsatellite markers enabling us to analyse the relatedness in "mixed" cultivated families, we studied the variability of breeding success in... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Huîtres; Marqueurs microsatellites; Reproduction; Sélection; Dérive génétique; Variabilité génétique; Génétique. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3481.pdf |
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| Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre. |
| Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène... |
| Tipo: Text |
Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf |
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| Boudry, Pierre; Leitao, Alexandra; Mccombie, Helen; Thiriot-quievreux, Catherine. |
| Cytogenetic abnormalities arising both in mitosis and meiosis are known to be common in bivalves. Here we review result obtained from the observations of somatic aneuploidy in different populations of the commercially important Pacific oyster, Crassostrea gigas. The oysters studied were either (1) produced in IFREMER hatcheries between 1986 and 1999, (some of these were part of the EU funded programme GENEPHYS); or (2) collected from wild populations along the French Atlantic coast. Chromosome numbers were scored from 30 mitotic metaphases in gill tissue per individual studied. Aneuploid cells (2n - X) were observed in all the 13 populations studied. A highly significant negative correlation was observed between the level of aneuploidy and growth, i.e.... |
| Tipo: Text |
Palavras-chave: Growth; Genetic; Aneuploidy; Crassostrea gigas; Pacific oysters. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3446.pdf |
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| Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine. |
| The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed. |
| Tipo: Text |
Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf |
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| Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre. |
| Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been... |
| Tipo: Text |
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf |
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| Normand, Julien; Ernande, Bruno; Boudry, Pierre. |
| Today, triploidy is the most common method used to genetically improve marine bivalve aquaculture production. In the Pacific oyster Crassostrea gigas, triploidy affects many traits. The most proeminent one is reproductive effort, which is greatly reduced compared to diploids. The combined effects of physiological trade-offs between survival, growth and reproduction together with the reduction of reproductive effort in triploids explains the global enhancement of yield observed. However, the sterility of triploid oysters is only partial and some triploid individuals can show normal gametogenesis (i.e. similar to diploids), especially under warm conditions. In diploid oysters, the heritability of reproductive effort has been shown to be relatively low, but a... |
| Tipo: Text |
Palavras-chave: Parentage analysis; Triploidy; Oyster; Quantitative genetics; Gametogenesis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4525.pdf |
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| Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie. |
| Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie. |
| Ano: 2005 |
URL: https://archimer.ifremer.fr/doc/2005/publication-654.pdf |
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| Degremont, Lionel; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Soletchnik, Patrick; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois. |
| In the scope of the Morest program, 3 series of bi-parental families of cupped oyster consisting of full and half-sib have been created in 2001, and then studied on a regular basis on several sites over the summer period. In October, major survival differences had been demonstrated between the 3 families. The average survival rate for the half-sib families monitored on the three sites varies from 17 to 97 %. Some of them showed high mortality for the three sites, indicating a strong genetic basis (h2 = 0.81 - 0.29) to the variations observed regarding survival. To a lesser extent, growth differences between the studied half-sib families indicate a weaker heritability for this character. .... |
| Tipo: Text |
Palavras-chave: Physiologie; Génétique; Naissain; Sélection; Croissance; Survie; Huîtres; Crassostrea gigas. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3490.pdf |
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| Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie. |
| P>Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf |
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| Corporeau, Charlotte; Fleury, Elodie; Moal, Jeanne; Boudry, Pierre; Favrel, Pascal; Huvet, Arnaud. |
| In summer, oysters suffer mortality that decimates this year about 60% of the population in France. We previously demonstrated a genetic basis for summer survival thatallowed us to produce resistant (R) and susceptible (S) oyster familiesby divergent selection (Samainet al., 2007). At the physiological level, R families can survive summer mortality because they are reproductively less active than S families (Fleury et al., 2008).In order to identify the molecular basis of the summer survival,a transcriptomicapproach on R and S families was performed using microarray analysis and 34 differentially-expressed genes were identified between R and S in the gonad (Fleury et al., in prep). Among these genes, we found an EST encoding a neuropeptide Y related... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00083/19424/17035.pdf |
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| Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean. |
| The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation... |
| Tipo: Text |
Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00311/42251/41692.pdf |
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| Borcier, Elodie; Morvezen, Romain; Boudry, Pierre; Miner, Philippe; Charrier, Gregory; Laroche, Jean; Hegaret, Helene. |
| Dinoflagellates of the genus Alexandrium are a major cause of harmful algal blooms (HABs) that have increasingly disrupted coastal ecosystems for the last several decades. Microalgae from the genus Alexandrium are known to produce paralytic shellfish toxins (PST) but also bioactive extracellular compounds (BEC) that can display cytotoxic, allelopathic, ichtyotoxic or haemolytic effects upon marine organisms. The objective of this experimental study was to assess the effects of PST and BEC produced by A. minutum upon juvenile great scallops Pecten maximus. Scallops were exposed for one week to two different strains of A. minutum, the first producing both PST and BEC and the second producing only BEC. Escape response to starfish, daily shell growth,... |
| Tipo: Text |
Palavras-chave: Harmful Algal Bloom (HAB); Alexandrium minutum; Pecten maximus; Paralytic Shellfish Toxins (PST); Extracellular compounds; Behaviour; Histology. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00368/47878/47893.pdf |
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| Goyard, Emmanuel; Arnaud, Sophie; Vonau, Vincent; Bishoff, Vincent; Mouchel, Olivier; Pham, Dominique; Wyban, Jim; Boudry, Pierre. |
| The Latin American shrimp Litopenaeus stylirostris was introduced in three different Pacific islands (Tahiti, New Caledonia via Tahiti, and Hawaii) and hatchery-propagated for 7-25 generations to develop shrimp farming based on these domesticated stocks. Three microsatellite markers have been used in an attempt to assess the genetic bases of the populations available to start a selective breeding program. The comparison of eight hatchery stocks (five New Caledonian, two Hawaiian and one Tahitian stocks) and one wild Ecuadorian population showed a much lower variability in the domesticated stocks than in the wild population, especially in New Caledonia and Tahiti (2-3.7 vs. 14-27 alleles per locus; 20-60% vs. 90% expected heterozygosity). The Tahitian and... |
| Tipo: Text |
Palavras-chave: Microsatellite marker; Genetic variability; Domestication; Litopenaeus stylirostris; Shrimp. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-596.pdf |
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| Lapegue, Sylvie; Heurtebise, Serge; Cornette, Florence; Brizard, Raphael; Sharbel, Tim; Boudry, Pierre. |
| L'ostréiculture française réalise une production très largement extensive, basée sur le recrutement de juvéniles dans le milieu naturel et la production sur des sites côtiers. Ainsi, l’huître est l’une des rares espèces cultivées à être encore considéré par le consommateur comme un produit alimentaire totalement « naturel ». L'utilisation de la polyploïdisation a permis d'ouvrir une nouvelle voie dans cette filière, en permettant d'obtenir des animaux à croissance plus rapide, plus homogènes et stériles. Cette dernière caractéristique est un gage de qualité gustative et permet aussi de positionner ce produit en priorité sur les mois en "r" traditionnellement bannis à cause de la présence de laitance. Le but principal de la triploïdisation a été atteint:... |
| Tipo: Text |
Palavras-chave: Génétique; Triploïdisation; Tétraploïde; Impact; Environnement; Bivalvia; Crassostrea gigas. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00043/15460/12835.pdf |
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| Registros recuperados: 273 | |
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