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Registros recuperados: 67 | |
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Zakhour, Maha; Maalouf, Haifa; Di Bartolo, Ilaria; Haugarreau, Larissa; Le Guyader, Francoise; Ruvoen-clouet, Nathalie; Le Saux, Jean-claude; Ruggeri, Franco Maria; Pommepuy, Monique; Le Pendu, Jacques. |
Noroviruses (NoV) are major agents of acute gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Previous studies showed that some human strains bind to oyster tissues through carbohydrate ligands that are similar to their human receptors. Thus, based on presentation of shared norovirus carbohydrate ligands, oysters could selectively concentrate animal strains with increased ability to overcome species barriers. In comparison with human GI and GII strains, bovine GIII NoV strains, although frequently detected in bovine feces and waters of two estuaries of Brittany, were seldom detected in oysters grown in these estuaries. Characterization of the carbohydrate ligand from a new GIII strain indicated recognition of the... |
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Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12534/9527.pdf |
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Le Saux, Jean-claude; Pommepuy, Monique; Caprais, Marie-paule; Le Mennec, Cecile; Parnaudeau, Sylvain; Le Guyader, Francoise. |
Un des objectifs de ce projet est d'assurer un développement économique durable à l'ostréiculture, basé sur des technologies propres à définir des produits adaptés à la sécurité alimentaire. La production conchylicole est importante sur le marché européen et représente plus d'un tiers du marché mondial, soit plus de 775 000 tonnes/an, dont plus de 140 000 tonnes d'huîtres produites par la France. En Europe, le marché des coquillages représente plus de 456 M d'euros/an produits par 8 500 entreprises employant environ 40 000 personnes. Les huîtres à elles seules, représentent plus de 140 Md' euros/an. Le développement durable de cette activité passe par la maîtrise de la qualité du produit et en particulier de sa qualité sanitaire. En fait, le libre échange... |
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Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00044/15545/12931.pdf |
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Dubois, E; Le Guyader, Soizick; Haugarreau, Larissa; Kopecka, H; Cormier, M; Pommepuy, Monique. |
Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples, Amplified viral sequences were analyzed bg restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples, The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were... |
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Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00336/44720/44851.pdf |
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Coutard, Francois; Lozach, Solen; Pommepuy, Monique; Hervio Heath, Dominique. |
A real-time reverse transcription-PCR method was developed to determine whether the recovery of culturability of viable but nonculturable (VBNC) Vibrio parahaemolyticus induced the expression of virulence genes coding for the thermostable direct hemolysin and for type III secretion system 2 (TTSS2). The culturability of clinical strain Vp5 of V. parahaemolyticus in artificial seawater at 4°C was monitored, and the VBNC state was obtained. One day after entry into the VBNC state, temperature upshifts to 20 and 37°C allowed the recovery of culturability. Standard curves for the relative quantification of expression of the housekeeping genes rpoS, pvsA, fur, and pvuA; the tdh2 gene; and the TTSS2 genes (VPA1354, VPA1346, and VPA1342) were established. The... |
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Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2787.pdf |
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Pommepuy, Monique. |
The study 'admission capacity of the marine environment" was conducted by IFREMER 1980 to 1983 in the Brest Roadstead at the request of the Ministry of Environment. This study was financed jointly by the Board of Health and IFREMER. The problem was the study of the fate of bacterial discharges in a sea subjected to tides. The selected approach consisted to simulate discharges from the principal pollution sources and to calculate the theoretical dilution of fecal bacteria in the Northern zone of the Roadstead. Spreading coefficients were determined by "on the site" measurements of parameters which may be considered as conservative in defined periods (e.g. ammonium in winter). Results show that in the conditions of the styay, the disappearance of fecal... |
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Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00054/16572/14067.pdf |
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Le Guyader, Soizick; Pommepuy, Monique; Atmar, Robert. |
Human and animal fecal wastes and urine contain a large number of different viruses that can enter the environment through the discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. Viruses are present in shellfish in very low numbers. Nevertheless, they are present in sufficient quantities to pose a health risk as presented. This low level of contamination has made it necessary to develop highly sensitive viral extraction methods to ensure virus recovery from shellfish tissues. The most common route for accidental contamination is after heavy... |
Tipo: Text |
Palavras-chave: Human enteric viruses; Shellfish; Sewage; Persistence; Flux. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00015/12606/15279.pdf |
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Registros recuperados: 67 | |
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