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Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples ArchiMer
Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf
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Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis ArchiMer
Martenot, Claire; Gervais, Ophelie; Chollet, Bruno; Houssin, Maryline; Renault, Tristan.
Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this way, an OsHV-1 challenge was performed and oysters were monitored at three times post injection to investigate viral infection and host response: 2h (early after viral injection in the adductor muscle), 24h (intermediate time), and 48h (just before first oyster mortality record). Virus infection, associated with high cumulative mortality rates (74% and 100%), was...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00386/49715/50244.pdf
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The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA ArchiMer
Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H..
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf
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Toward reference intervals for shellfish: An illustrative case of feeding and respiratory activities in the Pacific cupped oyster, Crassostrea gigas ArchiMer
François, Cyrille; Haure, Joel; Billy, Jean-christophe; Renault, Tristan.
Background The Quality Assurance and Laboratory Standards Committee of the American Society for Veterinary Clinical Pathology and the guidelines of the Clinical and Laboratory Standards Institute provide a framework for establishing reference intervals of physiological parameters in reputedly healthy individuals, humans, and terrestrial animals, respectively. This framework was applied for the first time to the Pacific cupped oyster, Crassostrea gigas. Reference intervals (RIs) would, first, be of interest for research purposes, including pathophysiology studies. RI determination is the first step before considering the use of RIs for field applications by farmers and marine shellfish health services. Objectives The purpose of this study was to propose...
Tipo: Text Palavras-chave: Bivalvia; Clearance rate; Health; Ostreidae; Oxygen consumption rate; Reference values.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00627/73913/73286.pdf
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New Insight for the Genetic Evaluation of Resistance to Ostreid Herpesvirus Infection, a Worldwide Disease, in Crassostrea gigas ArchiMer
Degremont, Lionel; Lamy, Jean-baptiste; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
The Pacific oyster, Crassostrea gigas, is the most important commercial oyster species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster, and numerous mortality outbreaks related to this pathogen are now reported worldwide. To assess the genetic basis of resistance to OsHV-1 infection in spat C. gigas and to facilitate breeding programs for such a trait, if any exist, we compared the mortality of half- and full-sib families using three field methods and a controlled challenge by OsHV-1 in the laboratory. In the field, three methods were tested: (A) one family per bag; (B) one family per small soft mesh bag and all families inside one bag; (C) same as the previous methods but...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/38066/36178.pdf
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Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf
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Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France ArchiMer
Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf
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New Trends in Important Diseases Affecting the Culture of Fish and Molluscs in the ICES Area 2002– 2015 ArchiMer
Alfjorden, A; Areskog, M; Bruno, D; Carnegie, R; Cheslett, D; Feist, S; Ford, S; Jones, S; Lillehaug, Atle; Madsen, L; Renault, Tristan; Ruane, N; Vennerstrom, P.
The ICES Working Group on Pathology and Diseases of Marine Organisms (WGPDMO) provides annual reviews of national reports on the disease status of wild and farmed fish and molluscs in the ICES area. In 2004, the group published a first report collating this information from 1998-2002. This second report aims to provide an update on the status of the major diseases described in the original report and also to provide an overview of new diseases which have emerged since the previous report was published.
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00585/69731/67620.pdf
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Recherches d'ADN d'hepès virus infectant les mollusques dans les populations d'huîtres en milieu naturel et dans leur environnement ArchiMer
Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan.
The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural...
Tipo: Text Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf
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Study of atrazine effects on Pacific oyster, Crassostrea gigas, haemocytes ArchiMer
Gagnaire, Beatrice; Renault, Tristan; Bouilly, Karine; Lapegue, Sylvie; Thomas-guyon, H.
Shellfish farming is an important economic activity around the world. This activity often takes place in areas subjected to various recurring pollutions. The recrudescent use of herbicides in agriculture including atrazine implies pollutant transfer towards aquatic environment in estuarine areas. Harmful effects of such substances on animals in marine environment, particularly on cultured bivalves, are poorly documented. Bivalve molluscs such as mussels and oysters have been postulated as ideal indicator organisms because of their way of life. They filter large volumes of seawater and may therefore accumulate and concentrate eontaJ.11inants within their tissues. Moreover, development of techniques allowing effect analysis of such compounds on bivalve...
Tipo: Text Palavras-chave: Crassostrea gigas; Haemocytes; Flow cytometry; Atrazine; Cellular activities; Toxicity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2827.pdf
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Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis ArchiMer
Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf
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Experimental infection of Pacific oyster Crassostrea gigas spat by ostreid herpesvirus 1: demonstration of oyster spat susceptibility ArchiMer
Schikorski, David; Renault, Tristan; Saulnier, Denis; Faury, Nicole; Moreau, Pierrick; Pepin, Jean-francois.
In 2008 and 2009, acute mortalities occurred in France among Pacific cupped oyster, Crassostrea gigas, spat. Different hypothesis including the implication of environmental factors, toxic algae and/or pathogens have been explored. Diagnostic tests indicated that OsHV-1 including a particular genotype, termed OsHV-1 mu Var, was detected in most of samples and especially in moribund oysters with the highlighting of virus particles looking like herpes viruses by TEM examination. In this study, an experimental protocol to reproduce OsHV-1 infection in laboratory conditions was developed. This protocol was based on the intramuscular injection of filtered (0.22 mu m) tissue homogenates prepared from naturally OsHV-1 infected spat collected on French coasts...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00037/14795/12104.pdf
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Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf
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Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf
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Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters ArchiMer
Diaz, Seila; Renault, Tristan; Villalba, Antonio; Carballal, María Jesús.
Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca2+...
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Ano: 2011 URL: https://archimer.ifremer.fr/doc/00043/15468/12846.pdf
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Facteurs de risque d'apparition et d'émergence des maladies infectieuses en aquaculture ArchiMer
Renault, Tristan; Guichard, B..
In the last few years, the global increase of aquatic animal production and farmed species has been associated with an increase in the number and spread of infectious diseases. In 2002-2003, 62 cases of emerging diseases were reported in aquatic animals, two thirds in aquacultured species, mainly in Europe and Northern America. A disease is emerging when its incidence significantly increases in a given time, place and population. To emerge, a disease has to be introduced in a free zone or a free population, and animals must be exposed to favouring transmission factors. The risks of introducing new diseases are mainly associated to movements of infected animals (farmed or feral, dead or alive), contaminated farming gear or transport media, evolution of...
Tipo: Text Palavras-chave: Facteurs d'exposition; Animaux aquatiques; Maladies émergentes.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2977.pdf
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Rapport d'activité 1994 du Laboratoire Génétique, Aquaculture et Pathologie de La Tremblade - Bouin ArchiMer
Gerard, Andre; Baud, Jean-pierre; Renault, Tristan; Goulletquer, Philippe.
Rapport d'activité 1994 du Laboratoire Génétique, Aquaculture et Pathologie de La Tremblade et Bouin.
Tipo: Text Palavras-chave: Génétique; Pathologie; Invertébrés marins; Rapport Activité.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00071/18252/15815.pdf
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Comparative Proteomics of Ostreid Herpesvirus 1 and Pacific Oyster Interactions With Two Families Exhibiting Contrasted Susceptibility to Viral Infection ArchiMer
Leprêtre, Maxime; Faury, Nicole; Segarra, Amelie; Claverol, Stéphane; Degremont, Lionel; Palos-ladeiro, Mélissa; Armengaud, Jean; Renault, Tristan; Morga, Benjamin.
Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and...
Tipo: Text Palavras-chave: Proteomics; Interactions; Antiviral response; OsHV-1; Pacific oysters.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00669/78075/80307.pdf
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Juvenile oyster disease (JOD) in American oysters, Crassostrea virginica, reared in France. Detection of abnormal calcium deposits in gill and mantle tissues ArchiMer
Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared during 1992 and 1993, to test their growth performances. These assays were performed to investigate the possible replacement of Crassostrea gigas with a non indigenous species, Crassostrea virginica. During the summer of 1993, sporadic mass mortalities (80-90%) occurred among young, Crassastrea virginica, oysters reared in the IFREMER nursery in La Tremblade (Charente Maritime).
Tipo: Text Palavras-chave: Histology; Crassostrea virginica; Mortality; Oysters.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/acte-2975.pdf
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Episodes de mortalité massive de moules bleues observés en 2014 dans les Pertuis charentais ArchiMer
Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan.
Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen
Tipo: Text Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf
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