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| Registros recuperados: 992 | |
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| Moukette,Bruno Moukette; Pieme,Constant Anatole; Njimou,Jacques Romain; Biapa,Cabral Prosper Nya; Marco,Bravi; Ngogang,Jeanne Yonkeu. |
| BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose... |
| Tipo: Journal article |
Palavras-chave: Antioxidant; Radical scavenging; HPLC; Monodora myristica; Non-timber forest product. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100015 |
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| COURT,FELIPE A; ÁLVAREZ,JAIME. |
| In this essay, we show that several anatomical features of the axon, namely, microtubular content, caliber and extension of sprouts, correlate on a local basis with the particular condition of the glial cell, i.e., the anatomy of axons is dynamic, although it is seen usually in its `normal' state. The occurrence of ribosomes and messenger RNAs in the axon suggests that axoplasmic proteins are most likely synthesized locally, at variance with the accepted notion that they are supplied by the cell body. We propose that the supporting cell (oligodendrocyte or Schwann cell) regulates the axonal phenotype by fine-tuning the ongoing axonal protein synthesis. |
| Tipo: Journal article |
Palavras-chave: Axon; Protein synthesis; Nerve regeneration; Schwann cell; Wld s. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400009 |
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| CALÁBRIA,LUCIANA KAREN; TEIXEIRA,RENATA ROLAND; COELHO GONÇALVES,SYBELLI MAGDA; PASSOS LIMA,ANDREIA BARCELOS; SANTOS,ANA ALICE DINIZ DOS; MARTINS,ANTÔNIO ROBERTO; ESPINDOLA,FOUED SALMEN. |
| The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain. |
| Tipo: Journal article |
Palavras-chave: Antigen retrieval; Microwave oven; Steam cooker. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002 |
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| Chen,Haide; Li,Yang; Lin,Xijuan; Cui,Di; Cui,Chun; Li,Hui; Xiao,Lei. |
| BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA-/-hESCs did... |
| Tipo: Journal article |
Palavras-chave: HESCs; CIITA; TALENs; Immune rejection. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059 |
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| PALACIOS,ADRIÁN G.; BACIGALUPO,JUAN. |
| One of the most eminent neuroscientists recently passed away in Paris. Professor Francisco Varela was a scholar that approached science with a remarkably broad and integrative perspective, deeply contributing to a diversity of fields, from mathematics to epistemology, from immunology to neuroscience. He was strongly influenced by Buddhism and actively participated in unraveling the relationship between science and spirituality. This article introduces a special edition of Biological Research dedicated to the memory of this great man. It contains a collection of valuable contributions by various authors who collaborated with Varela at different moments of his outstanding scientific career. Their articles cover most of the fields in which he made... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000100002 |
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| Vigil,Pilar; Orellana,Renán F; Cortés,Manuel E. |
| Spermatozoon acrosome reaction is an exocytotic event of the utmost importance for the development of mammalian fertilisation. Current evidence shows that the triggering of the acrosome reaction (AR) could be regulated by the action of diverse compounds, namely, metabolites, neurotransmitters and hormones. The aim of the present review is to describe the modulating effects of several compounds that have been classified as inductors or inhibitors of acrosome reaction. Among AR inductors, it is necessary to mention progesterone, angiotensin II, atrial natriuretic peptide, cathecolamines, insulin, leptin, relaxin and other hormones. Regarding the inhibitors, oestradiol and epidermal growth factor are among the substances that retard AR. It is worth mentioning... |
| Tipo: Journal article |
Palavras-chave: Capacitation; Gamma-aminobutyric acid; Hormone modulation; Steroids; Spermatozoon acrosome reaction. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200007 |
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| ZÓCALO,YANINA; BIA,DANIEL; LLUBERAS,SEBASTIÁN; ARMENTANO,RICARDO L. |
| Background: The characterization of the dynamic process of veins walls is essential to understand venous functioning under normal and pathological conditions. However, little work has been done on dynamic venous properties. Aim: To characterize vein compliance (C), viscosity (η), peak-strain (W St) and dissipated (W D) energy, damping (ξ), and their regional differences in order to evalúate their role in venous functioning during volume-pressure overloads. Methods: In a mock circulation, pressure (P) and diameter (D) of different veins (anterior cava, jugular and femoral; from 7 sheep), were registered during cyclical volume-pressure pulses. From the P-D relationship, C, W St and ξ (at low and high P-D leveis), η and W D... |
| Tipo: Journal article |
Palavras-chave: Compliance; Damping; Energetic; Vascular physiology; Venous wall; Viscosity. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200012 |
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| CHARRIER,MAR YVONNE; FONTY,GERARD; GAILLARD-MARTINIE,BRIGITTE; AINOUCHE,KADER; ANDANT,GERARD. |
| The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared ≥ 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium,... |
| Tipo: Journal article |
Palavras-chave: Clostridium; Edible snails; Enterobacteriaceae; Enterococci; Gut microbiota; Lactococci. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010 |
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| Zhong,Gang; Xiong,Xingao. |
| BACKGROUND: The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. miR-205 was reported to be upregulated in laryngeal squamous cell carcinoma (LSCC) tissues, however, the mechanisms by which miR-205 functions as a regulator of LSCC are largely unknown RESULTS: In this study, Real-time qPCR and Western blot assay showed that expression of miR-205 was upregulated and expression of cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was downregulated in LSCC tissues. The expression levels of miR-205 were negatively related to those of CDK2AP1 in LSCC tissues and cell lines. Moreover, we found that miR-205 was the upstream regulator of CDK2AP1 and could suppress the CDK2AP1 expression in LSCC cells.... |
| Tipo: Journal article |
Palavras-chave: MiR-205; Laryngeal squamous cell carcinoma (LSCC); CDK2AP1; C-Myc; CyclinD1. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100060 |
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| VERA,JORGE; VALENZUELA,BEATRIZ; ROTH,MÓNICA J; LEÓN,ÓSCAR. |
| Processing of viral DNA by retroviral integrase leaves a dinucleotide single-strand overhang in the unprocessed strand. Previous studies have stressed the importance of the 5' single-stranded (ss) tail in the integration process. To characterize the ss-tail binding site on M-MuLV integrase, we carried out crosslinking studies utilizing a disintegration substrate that mimics the covalent intermediate formed during integration. This substrate carried reactive groups at the 5' ss tail. A bromoacetyl derivative with a side chain of 6 A was crosslinked to the mutant IN 106-404, which lacks the N-terminal domain, yielding a crosslinked complex of 50 kDa. Treatment of IN 106-404 with N-ethylmaleimide (NEM) prevented crosslinking, suggesting that Cys209 was... |
| Tipo: Journal article |
Palavras-chave: Integrase; Retrovirus; Crosslinking. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100009 |
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| Garrido,Osvaldo; Letelier,René; Rosas,Carlos; Fuenzalida,Marcela; Ferreira,Arturo; Lemus,David. |
| Tumor resistance to traditional cancer treatments poses an important challenge to modern science. Thus, angiogenesis inhibition is an important emerging cancer treatment. Many drugs are tested and corticosteroids have shown interesting results. Herein we investigate the effect on microvessel density, survival time and tumoral volume of mice with TA3-MTX-R tumors. Twenty six mice were inoculated with lxlO6 tumor cells, 4-5 days after injection, six mice were injected with PBS (group A) and twenty mice were treated with p-met (group B). All animals from Group A died on day 22. Group B was divided into Bl (treated discontinued) and B2 (treated daily) and observed until day 88. All mice were processed for histo-immunohistochemical analysis and the blood... |
| Tipo: Journal article |
Palavras-chave: Angiogenesis; Betamethasone treatment; Metastasis. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300008 |
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| Registros recuperados: 992 | |
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