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An optically coupled power stimulus isolation unit with high voltage and fast rise time output BJMBR
Brasil,R.O.; Leal-Cardoso,J.H..
Recent technological developments have created new devices that could improve and simplify the construction of stimulus isolators. HEXFET transistors can switch large currents and hundreds of volts in nanoseconds. The newer opto-isolators can give a pulse rise time of a few nanoseconds, with output compatible with MOSFET devices, in which delays are reduced to nanoseconds. Integrated DC/DC converters are now available. Using these new resources we developed a new electrical stimulus isolator circuit with selectable constant-current and constant-voltage modes, which are precise and easy to construct. The circuit works like a regulated power supply in both modes with output switched to zero or to free mode through an opto-isolator device. The isolator...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Electrical isolation; Electrical stimulation; Pulse stimulation; Stimulus isolator.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600013
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Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats BJMBR
Schröder,N.; de-Paris,F.; Roesler,R.; Medina,J.H.; Souza,D.O.; Izquierdo,I..
Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P<0.01), but not at 0, 15, or 60 min after training, and did not affect...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Glutamate receptor hippocampus parietal cortex long-term potentiation emotional learning; Memory.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200011
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Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats BJMBR
Veras,A.S.C.; Baptista,D.B.; dos Santos,N.J.; Thorpe,H.H.A.; Seraphim,P.M.; Florido Neto,A.R.; Teixeira,G.R..
Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). For eight weeks, animals were repeatedly exposed to cigarette smoke for 30 min or performed aerobic physical training either with or without the cigarette smoke inhalation protocol. Following these experiments, we analyzed prostate epithelial morphology and prostatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inflammation; Androgen receptor; Growth factors; Endurance exercise; BAX; BCL-2.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500601
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Low frequency of p53 mutations in cervical carcinomas among Brazilian women BJMBR
Pinheiro,N.A.; Villa,L.L..
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005
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MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL BJMBR
Gao,Fei; Tan,Yuan; Luo,Hong.
Systemic lupus erythematosus (SLE) is an autoimmune disease associated with an aberrant activation of immune cells partly due to the dysfunction of cytokines such as type I interferons (IFNs). Long non-coding RNA MALAT1 has been found to play a pathogenic role in SLE; however, the underlying mechanisms are still poorly understood. Bioinformatics analysis showed the up-regulation of type I IFN downstream effectors OAS2, OAS3, and OASL (OAS-like) in CD4+ T cells, CD19+ B cells, and CD33+ myeloid cells in patients with active SLE compared to healthy participants. In this study, peripheral blood mononuclear cells (PBMCs), CD19+ B, and CD4+ T cells were isolated from active SLE patients and healthy participants. PCR was performed to quantify MALAT1, OAS2, OAS3,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MALAT1; OAS2; OAS3; OASL; Systemic lupus erythematosus; Type I IFNs.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500603
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Distribution of biglycan and decorin in rat dental tissue BJMBR
Tenório,D.M.H.; Santos,M.F.; Zorn,T.M.T..
Biglycan and decorin are small leucine-rich proteoglycans that play several biological and structural roles in different tissues and organs. Several reports have indicated that biglycan participates in odontoblast and ameloblast differentiation and in the calcification process. In the present study we show that the expression of biglycan changes from within the ameloblasts and odontoblasts to the extracellular space according to the stage of animal development. In predentin and in the pulp space, however, biglycan was continually expressed throughout the period of investigation. In contrast, decorin was absent in odontoblasts and in ameloblasts and was exclusively expressed in predentin throughout the period of observation. In young rats, however, decorin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decorin; Biglycan; Odontoblasts; Predentin; Immunohistochemistry.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800012
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Comparison of the neurobiological effects of attribution retraining group therapy with those of selective serotonin reuptake inhibitors BJMBR
Wang,C.; Zhang,N.; Zhang,Y.L.; Zhang,J.; Yang,H.; Timothy,T.C..
The aim of this study was to compare the effectiveness of attribution retraining group therapy (ARGT) with selective serotonin reuptake inhibitors (SSRIs) in the treatment of major depressive disorder (MDD), generalized anxiety disorder (GAD), and obsessive-compulsive disorder (OCD). Subjects were sequentially recruited and randomized into two groups, one receiving ARGT (n = 63) and the other SSRIs (n = 66) for 8 weeks. Fifty-four ARGT outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 16) and 55 SSRI outpatients with MDD (n = 19), GAD (n = 19), and OCD (n = 17) completed the study. All subjects were assessed using the Hamilton Depression Scale and Hamilton Anxiety Scale before and after treatment. The 10-item Yale-Brown Obsessive Compulsive Scale...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Attribution retraining group therapy; Selective serotonin reuptake inhibitors; Neurobiological effects; Serotonin; Cortisol.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300318
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Respiratory panic disorder subtype and sensitivity to the carbon dioxide challenge test BJMBR
Valença,A.M.; Nardi,A.E.; Nascimento,I.; Zin,W.A.; Versiani,M..
The aim of the present study was to verify the sensitivity to the carbon dioxide (CO2) challenge test of panic disorder (PD) patients with respiratory and nonrespiratory subtypes of the disorder. Our hypothesis is that the respiratory subtype is more sensitive to 35% CO2. Twenty-seven PD subjects with or without agoraphobia were classified into respiratory and nonrespiratory subtypes on the basis of the presence of respiratory symptoms during their panic attacks. The tests were carried out in a double-blind manner using two mixtures: 1) 35% CO2 and 65% O2, and 2) 100% atmospheric compressed air, 20 min apart. The tests were repeated after 2 weeks during which the participants in the study did not receive any psychotropic drugs. At least 15 of 16 (93.7%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CO2; Anxiety; Breathing; Respiration.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700004
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Paraquat poisoning: an experimental model of dose-dependent acute lung injury due to surfactant dysfunction BJMBR
Silva,M.F.R.; Saldiva,P.H.N..
Since the most characteristic feature of paraquat poisoning is lung damage, a prospective controlled study was performed on excised rat lungs in order to estimate the intensity of lesion after different doses. Twenty-five male, 2-3-month-old non-SPF Wistar rats, divided into 5 groups, received paraquat dichloride in a single intraperitoneal injection (0, 1, 5, 25, or 50 mg/kg body weight) 24 h before the experiment. Static pressure-volume (PV) curves were performed in air- and saline-filled lungs; an estimator of surface tension and tissue works was computed by integrating the area of both curves and reported as work/ml of volume displacement. Paraquat induced a dose-dependent increase of inspiratory surface tension work that reached a significant two-fold...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paraquat; Experimental model; Acute lung injury; Pressure-volume curve; Respiratory work.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300018
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Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells BJMBR
Wang,W.; Zhu,H.; Zhang,H.; Zhang,L.; Ding,Q.; Jiang,H..
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role ofPPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were used to explore the effects ofPPM1D knockdown on BC cell proliferation and tumorigenesis. shRNA-mediated knockdown of PPM1D significantly inhibited cell growth and colony forming ability in the BC cell lines 5637 and T24. Flow cytometric analysis showed that PPM1D silencing increased the proportion of cells in the G0/G1 phase. Downregulation of PPM1Dalso inhibited 5637 cell tumorigenicity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein phosphatase magnesium/manganese-dependent 1D; Bladder cancer; Gene silencing; RNA interference; Proliferation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201044
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Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats BJMBR
Tavares,J.G.P.; Vasques,E.R.; Arida,R.M.; Cavalheiro,E.A.; Cabral,F.R.; Torres,L.B.; Menezes-Rodrigues,F.S.; Jurkiewicz,A.; Caricati-Neto,A.; Godoy,C.M.G.; Gomes da Silva,S..
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epilepsy; Heart; Acute myocardial infarction; Ischemia; Reperfusion.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200140
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Gross proteinuria is a strong risk predictor for cardiovascular mortality in Brazilian type 2 diabetic patients BJMBR
Cardoso,C.R.L.; Salles,G.F..
Increased proteinuria is recognized as a risk predictor for all-cause and cardiovascular mortality in diabetic patients; however, no study has evaluated these relationships in Brazilian patients. The aim of this study was to investigate the prognostic value of gross proteinuria for all-cause and cardiovascular mortalities and for cardiovascular morbidity in a cohort study of 471 type 2 diabetic individuals followed for up to 7 years. Several clinical, laboratory and electrocardiographic variables were obtained at baseline. The relative risks for all-cause, cardiovascular and cardiac mortalities and for cardiovascular and cardiac events associated with the presence of overt proteinuria (>0.5 g/24 h) were assessed by Kaplan-Meier survival curves and by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: All-cause mortality; Cardiovascular mortality; Cardiovascular morbidity; Proteinuria; Type 2 diabetes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800006
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The novel p.E89K mutation in the SRY gene inhibits DNA binding and causes the 46,XY disorder of sex development BJMBR
Cunha,J.L.; Soardi,F.C.; Bernardi,R.D.; Oliveira,L.E.C.; Benedetti,C.E.; Guerra-Junior,G.; Maciel-Guerra,A.T.; de Mello,M.P..
Male sex determination in humans is controlled by the SRY gene, which encodes a transcriptional regulator containing a conserved high mobility group box domain (HMG-box) required for DNA binding. Mutations in the SRY HMG-box affect protein function, causing sex reversal phenotypes. In the present study, we describe a 19-year-old female presenting 46,XY karyotype with hypogonadism and primary amenorrhea that led to the diagnosis of 46,XY complete gonadal dysgenesis. The novel p.E89K missense mutation in the SRY HMG-box was identified as a de novo mutation. Electrophoretic mobility shift assays showed that p.E89K almost completely abolished SRY DNA-binding activity, suggesting that it is the cause of SRY function impairment. In addition, we report the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gonadal dysgenesis; HMG-box; Missense mutation; Sex reversal SRY; Streak gonads; Testis determination.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400014
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Cytoadhesion of Plasmodium falciparum-infected erythrocytes and the infected placenta: a two-way pathway BJMBR
Costa,F.T.M.; Avril,M.; Nogueira,P.A.; Gysin,J..
Malaria is undoubtedly the world's most devastating parasitic disease, affecting 300 to 500 million people every year. Some cases of Plasmodium falciparum infection progress to the deadly forms of the disease responsible for 1 to 3 million deaths annually. P. falciparum-infected erythrocytes adhere to host receptors in the deep microvasculature of several organs. The cytoadhesion of infected erythrocytes to placental syncytiotrophoblast receptors leads to pregnancy-associated malaria (PAM). This specific maternal-fetal syndrome causes maternal anemia, low birth weight and the death of 62,000 to 363,000 infants per year in sub-Saharan Africa, and thus has a poor outcome for both mother and fetus. However, PAM and non-PAM parasites have been shown to differ...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmodium falciparum; Cytoadhesion; Pregnancy-associated malaria; Var2CSA gene.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200003
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Acute but not chronic metabolic acidosis potentiates the acetylcholine-induced reduction in blood pressure: an endothelium-dependent effect BJMBR
Celotto,A.C.; Ferreira,L.G.; Capellini,V.K.; Albuquerque,A.A.S.; Rodrigues,A.J.; Evora,P.R.B..
Metabolic acidosis has profound effects on vascular tone. This study investigated the in vivo effects of acute metabolic acidosis (AMA) and chronic metabolic acidosis (CMA) on hemodynamic parameters and endothelial function. CMA was induced by ad libitum intake of 1% NH4Cl for 7 days, and AMA was induced by a 3-h infusion of 6 M NH4Cl (1 mL/kg, diluted 1:10). Phenylephrine (Phe) and acetylcholine (Ach) dose-response curves were performed by venous infusion with simultaneous venous and arterial blood pressure monitoring. Plasma nitrite/nitrate (NOx) was measured by chemiluminescence. The CMA group had a blood pH of 7.15±0.03, which was associated with reduced bicarbonate (13.8±0.98 mmol/L) and no change in the partial pressure of arterial carbon dioxide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acid-base balance; Metabolic acidosis; Hemodynamic failure; Endothelium; Nitric oxide.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200604
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Identification of a novel UMOD mutation (c.163G>A) in a Brazilian family with autosomal dominant tubulointerstitial kidney disease BJMBR
Lopes,L.B.; Abreu,C.C.; Souza,C.F.; Guimaraes,L.E.R.; Silva,A.A.; Aguiar-Alves,F.; Kidd,K.O.; Kmoch,S.; Bleyer,A.J.; Almeida,J.R..
Autosomal dominant tubulointerstitial kidney disease (ADTKD) is characterized by autosomal dominant inheritance, progressive chronic kidney disease, and a bland urinary sediment. ADTKD is most commonly caused by mutations in the UMOD gene encoding uromodulin (ADTKD-UMOD). We herein report the first confirmed case of a multi-generational Brazilian family with ADTKD-UMOD, caused by a novel heterozygous mutation (c.163G>A, GGC→AGC, p.Gly55Ser) in the UMOD gene. Of 41 family members, 22 underwent genetic analysis, with 11 individuals found to have this mutation. Three affected individuals underwent hemodialysis, one peritoneal dialysis, and one patient received a kidney transplant from a family member later found to be genetically affected. Several younger...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronic kidney disease; Uromodulin kidney disease; Genetic mutation; Uromodulin; UMOD; Genetic kidney diseases.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300616
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Lymphocyte subpopulations during cytomegalovirus disease in renal transplant recipients BJMBR
Castro,S.M.; Sporleder,H.; Schröeder,R.; Santos,A.; Garcia,V.; Neumann,J.; Costa,S.C.B..
We have determined the number of circulating T, B and natural killer cells in renal transplant recipients in order to detect changes during cytomegalovirus (CMV) infections. Serial blood samples were taken from 61 patients on standard triple immunosuppression therapy (cyclosporin A, azathioprine and prednisone). Using two-color flow cytometry analysis, the absolute number of CD3+, CD4+, CD8+, CD19+, CD3+HLA-DR+ and CD16+56+ cells was determined. Forty-eight patients (78.7%) developed active CMV infection, and all of them subsequently recovered. Twenty of the infected patients (32.8%) presented symptoms compatible with CMV disease during the infectious process. The number of lymphocytes and their main subpopulations were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transplantation; Cytomegalovirus; Antigenemia; T lymphocytes.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600016
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Rimonabant improves metabolic parameters partially attributed to restoration of high voltage-activated Ca2+ channels in skeletal muscle in HFD-fed mice BJMBR
Chen,B.; Hu,N..
Cannabinoid type 1 receptor (CB1R) inhibition tends to be one of the promising strategies for the treatment of obesity and other related metabolic disorders. Although CB1R inhibition may cause adverse psychiatric effects including depression and anxiety, the investigation of the role of peripheral CB1R on weight loss and related metabolic parameters are urgently needed. We first explored the effect of rimonabant, a selective CB1R antagonist/inverse agonist, on some metabolic parameters in high fat-diet (HFD)-induced obesity in mice. Then, real-time PCR and electrophysiology were used to explore the contribution of high voltage-activated Ca2+ channels (HVACCs), especially Cav1.1, on rimonabant's effect in skeletal muscle (SM) in HFD-induced obesity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cannabinoid receptor type 1; Obesity; Rimonabant; Skeletal Muscle; HVA calcium channel.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600602
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Mitochondria, calcium and pro-apoptotic proteins as mediators in cell death signaling BJMBR
Smaili,S.S.; Hsu,Y.-T.; Carvalho,A.C.P.; Rosenstock,T.R.; Sharpe,J.C.; Youle,R.J..
Cellular Ca2+ signals are crucial in the control of most physiological processes, cell injury and programmed cell death through the regulation of a number of Ca2+-dependent enzymes such as phospholipases, proteases, and nucleases. Mitochondria along with the endoplasmic reticulum play pivotal roles in regulating intracellular Ca2+ content. Mitochondria are endowed with multiple Ca2+ transport mechanisms by which they take up and release Ca2+ across their inner membrane. During cellular Ca2+ overload, mitochondria take up cytosolic Ca2+, which in turn induces opening of permeability transition pores and disrupts the mitochondrial membrane potential (<FONT FACE=Symbol>Dy</FONT>m). The collapse of <FONT FACE=Symbol>Dy</FONT>m along...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ca2+; Mitochondrial Ca2+ uptake; Mitochondrial Ca2+ efflux; Permeability transition; Apoptosis; Bcl-2 family; Bax and apoptosis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200004
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Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis BJMBR
Batcioglu,K.; Gul,M.; Uyumlu,A.B.; Esrefoglu,M..
The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 µg/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 ± 2.29, 20.89 ± 10.13, 11.52 ± 4.60, 18.69 ± 8.56, and 8.58 ± 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 ± 0.83, 1.09 ± 0.35, 2.05 ± 0.91, 1.70 ± 0.60,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute pancreatitis; Liver injury; Oxidative damage; Pentoxifylline; L-NAME; L-arginine.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900001
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